Q96RP7 (G3ST4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactose-3-O-sulfotransferase 4
Short name=Gal3ST-4
EC=2.8.2.-

Alternative name(s):
Beta-galactose-3-O-sulfotransferase 4
Gal-beta-1,3-GalNAc 3'-sulfotransferase

Gene names

Name:	GAL3ST4
ORF Names:	PP6968

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc. Ref.1
Cofactor	Manganese.
Pathway	Protein modification; carbohydrate sulfation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node. Ref.1
Sequence similarities	Belongs to the galactose-3-O-sulfotransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6-7.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Non-traceable author statement Ref.1. Source: UniProtKB oligosaccharide metabolic process Non-traceable author statement Ref.1. Source: UniProtKB proteoglycan biosynthetic process Non-traceable author statement Ref.1. Source: UniProtKB sulfur compound metabolic process Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	3'-phosphoadenosine 5'-phosphosulfate binding Non-traceable author statement Ref.1. Source: UniProtKB galactose 3-O-sulfotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: InterPro proteoglycan sulfotransferase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Galactose-3-O-sulfotransferase 4		PRO_0000085209
Regions
	Topological domain	1 – 18	18	Cytoplasmic Potential
	Transmembrane	19 – 39	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	40 – 486	447	Lumenal Potential
Amino acid modifications
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	353	1	R → Q. Corresponds to variant rs3800952 [ dbSNP | Ensembl ].		VAR_021989
	Natural variant	467	1	A → V. Ref.2 Ref.7 Corresponds to variant rs3823646 [ dbSNP | Ensembl ].		VAR_033736
Experimental info
	Sequence conflict	137	1	M → L in BAB13977. Ref.3
	Sequence conflict	201 – 204	4	RGDH → VGTT in AAL55759. Ref.2
	Sequence conflict	441	1	S → R in BAB13977. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q96RP7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2D731D16B191CC3C	FASTA	486	54,166
	10 20 30 40 50 60 MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ PSAPSLRPAL 70 80 90 100 110 120 PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP ARYQFGYPKL FQASRVKGYR 130 140 150 160 170 180 PQGGGTQLPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR 190 200 210 220 230 240 KSPSLAAFLA NPRGFYRPGA RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL 250 260 270 280 290 300 QVLPSGAGPR AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL 310 320 330 340 350 360 VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA EDRQLTARAR 370 380 390 400 410 420 AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR EALAKHCLVG GEASDPKYIT 430 440 450 460 470 480 DRRFRPFQFG SAKVLGYILR SGLSPQDQEE CERLATPELQ YKDKLDAKQF PPTVSLPLKT SRPLSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-glycans." Seko A., Hara-Kuge S., Yamashita K. J. Biol. Chem. 276:25697-25704(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-467.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[5]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-467. Tissue: Placenta.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF316113 mRNA. Translation: AAK73365.1. AF289575 mRNA. Translation: AAL55759.1. AK022178 mRNA. Translation: BAB13977.1. AL833824 mRNA. Translation: CAD38686.2. CH236956 Genomic DNA. Translation: EAL23847.1. CH471091 Genomic DNA. Translation: EAW76575.1. CH471091 Genomic DNA. Translation: EAW76577.1. CH471091 Genomic DNA. Translation: EAW76579.1. BC012976 mRNA. Translation: AAH12976.1.
IPI	IPI00411637.
RefSeq	NP_078913.3. NM_024637.4.
UniGene	Hs.44856.
3D structure databases
ProteinModelPortal	Q96RP7.
ModBase	Search...
PTM databases
PhosphoSite	Q96RP7.
Polymorphism databases
DMDM	47116570.
Proteomic databases
PaxDb	Q96RP7.
PRIDE	Q96RP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360039; ENSP00000353142; ENSG00000197093. ENST00000413800; ENSP00000400451; ENSG00000197093.
GeneID	79690.
KEGG	hsa:79690.
UCSC	uc003utt.3. human.
Organism-specific databases
CTD	79690.
GeneCards	GC07M099758.
HGNC	HGNC:24145. GAL3ST4.
HPA	HPA038137.
MIM	608235. gene.
neXtProt	NX_Q96RP7.
PharmGKB	PA134921067.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG26344.
HOVERGEN	HBG051681.
InParanoid	Q96RP7.
KO	K09677.
OMA	FFSIVRD.
OrthoDB	EOG4BK546.
PhylomeDB	Q96RP7.
Enzyme and pathway databases
UniPathway	UPA00353.
Gene expression databases
ArrayExpress	Q96RP7.
Bgee	Q96RP7.
CleanEx	HS_GAL3ST4.
Genevestigator	Q96RP7.
GermOnline	ENSG00000197093. Homo sapiens.
Family and domain databases
InterPro	IPR009729. Gal-3-0_sulfotransfrase. [Graphical view]
PANTHER	PTHR14647. PTHR14647. 1 hit.
Pfam	PF06990. Gal-3-0_sulfotr. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	79690.
NextBio	68960.
SOURCE	Search...

Entry information

Entry name

G3ST4_HUMAN

Accession

Primary (citable) accession number: Q96RP7
Secondary accession number(s): A4D2A8 , D6W5U5, Q8N3P7, Q8WZ17, Q96E33, Q9HA78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.