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Protein

Galactose-3-O-sulfotransferase 4

Gene

GAL3ST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc.1 Publication

Cofactori

pH dependencei

Optimum pH is 6-7.

Pathway: carbohydrate sulfation

This protein is involved in the pathway carbohydrate sulfation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway carbohydrate sulfation and in Protein modification.

GO - Molecular functioni

  • 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
  • galactose 3-O-sulfotransferase activity Source: UniProtKB
  • galactosylceramide sulfotransferase activity Source: InterPro
  • proteoglycan sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • cell-cell signaling Source: UniProtKB
  • glycoprotein metabolic process Source: UniProtKB
  • oligosaccharide metabolic process Source: UniProtKB
  • proteoglycan biosynthetic process Source: UniProtKB
  • sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

UniPathwayiUPA00353.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-3-O-sulfotransferase 4 (EC:2.8.2.-)
Short name:
Gal3ST-4
Alternative name(s):
Beta-galactose-3-O-sulfotransferase 4
Gal-beta-1,3-GalNAc 3'-sulfotransferase
Gene namesi
Name:GAL3ST4
ORF Names:PP6968
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:24145. GAL3ST4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini40 – 486447LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134921067.

Polymorphism and mutation databases

BioMutaiGAL3ST4.
DMDMi47116570.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Galactose-3-O-sulfotransferase 4PRO_0000085209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ96RP7.
PRIDEiQ96RP7.

PTM databases

PhosphoSiteiQ96RP7.

Expressioni

Tissue specificityi

Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node.1 Publication

Gene expression databases

BgeeiQ96RP7.
CleanExiHS_GAL3ST4.
ExpressionAtlasiQ96RP7. baseline and differential.
GenevisibleiQ96RP7. HS.

Organism-specific databases

HPAiHPA038137.

Interactioni

Protein-protein interaction databases

BioGridi122811. 1 interaction.
IntActiQ96RP7. 1 interaction.
STRINGi9606.ENSP00000353142.

Structurei

3D structure databases

ProteinModelPortaliQ96RP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26344.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ96RP7.
KOiK09677.
OMAiRGDHYAR.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ96RP7.
TreeFamiTF314802.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96RP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ
60 70 80 90 100
PSAPSLRPAL PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP
110 120 130 140 150
ARYQFGYPKL FQASRVKGYR PQGGGTQLPF HILCHHMRFN LKEVLQVMPS
160 170 180 190 200
DSFFFSIVRD PAALARSAFS YYKSTSSAFR KSPSLAAFLA NPRGFYRPGA
210 220 230 240 250
RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL QVLPSGAGPR
260 270 280 290 300
AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL
310 320 330 340 350
VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA
360 370 380 390 400
EDRQLTARAR AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR
410 420 430 440 450
EALAKHCLVG GEASDPKYIT DRRFRPFQFG SAKVLGYILR SGLSPQDQEE
460 470 480
CERLATPELQ YKDKLDAKQF PPTVSLPLKT SRPLSP
Length:486
Mass (Da):54,166
Last modified:December 1, 2001 - v1
Checksum:i2D731D16B191CC3C
GO
Isoform 2 (identifier: Q96RP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MGPLSPARTL...FLKTHKSGSS → MTPRSPTSAF...TPALGRALPE
     81-142: Missing.

Note: No experimental confirmation available.
Show »
Length:424
Mass (Da):46,588
Checksum:i43AFFE8F564E79A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371M → L in BAB13977 (PubMed:14702039).Curated
Sequence conflicti201 – 2044RGDH → VGTT in AAL55759 (PubMed:15498874).Curated
Sequence conflicti441 – 4411S → R in BAB13977 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti353 – 3531R → Q.
Corresponds to variant rs3800952 [ dbSNP | Ensembl ].
VAR_021989
Natural varianti467 – 4671A → V.2 Publications
Corresponds to variant rs3823646 [ dbSNP | Ensembl ].
VAR_033736

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8080MGPLS…KSGSS → MTPRSPTSAFRSCTAVSSCL CPSSWPWLHLSVTSPFPVPH GPSLSCQDAAALGTSEPGGG SGSLHDHWLCTPALGRALPE in isoform 2. 1 PublicationVSP_057006Add
BLAST
Alternative sequencei81 – 14262Missing in isoform 2. 1 PublicationVSP_057007Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316113 mRNA. Translation: AAK73365.1.
AF289575 mRNA. Translation: AAL55759.1.
AK022178 mRNA. Translation: BAB13977.1.
AK301591 mRNA. Translation: BAG63080.1.
AL833824 mRNA. Translation: CAD38686.2.
AC073842 Genomic DNA. No translation available.
CH236956 Genomic DNA. Translation: EAL23847.1.
CH471091 Genomic DNA. Translation: EAW76575.1.
CH471091 Genomic DNA. Translation: EAW76577.1.
CH471091 Genomic DNA. Translation: EAW76579.1.
BC012976 mRNA. Translation: AAH12976.1.
CCDSiCCDS5688.1. [Q96RP7-1]
RefSeqiNP_078913.3. NM_024637.4. [Q96RP7-1]
UniGeneiHs.44856.

Genome annotation databases

EnsembliENST00000360039; ENSP00000353142; ENSG00000197093. [Q96RP7-1]
ENST00000413800; ENSP00000400451; ENSG00000197093. [Q96RP7-1]
GeneIDi79690.
KEGGihsa:79690.
UCSCiuc003utt.3. human. [Q96RP7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316113 mRNA. Translation: AAK73365.1.
AF289575 mRNA. Translation: AAL55759.1.
AK022178 mRNA. Translation: BAB13977.1.
AK301591 mRNA. Translation: BAG63080.1.
AL833824 mRNA. Translation: CAD38686.2.
AC073842 Genomic DNA. No translation available.
CH236956 Genomic DNA. Translation: EAL23847.1.
CH471091 Genomic DNA. Translation: EAW76575.1.
CH471091 Genomic DNA. Translation: EAW76577.1.
CH471091 Genomic DNA. Translation: EAW76579.1.
BC012976 mRNA. Translation: AAH12976.1.
CCDSiCCDS5688.1. [Q96RP7-1]
RefSeqiNP_078913.3. NM_024637.4. [Q96RP7-1]
UniGeneiHs.44856.

3D structure databases

ProteinModelPortaliQ96RP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122811. 1 interaction.
IntActiQ96RP7. 1 interaction.
STRINGi9606.ENSP00000353142.

PTM databases

PhosphoSiteiQ96RP7.

Polymorphism and mutation databases

BioMutaiGAL3ST4.
DMDMi47116570.

Proteomic databases

PaxDbiQ96RP7.
PRIDEiQ96RP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360039; ENSP00000353142; ENSG00000197093. [Q96RP7-1]
ENST00000413800; ENSP00000400451; ENSG00000197093. [Q96RP7-1]
GeneIDi79690.
KEGGihsa:79690.
UCSCiuc003utt.3. human. [Q96RP7-1]

Organism-specific databases

CTDi79690.
GeneCardsiGC07M099790.
HGNCiHGNC:24145. GAL3ST4.
HPAiHPA038137.
MIMi608235. gene.
neXtProtiNX_Q96RP7.
PharmGKBiPA134921067.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG26344.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ96RP7.
KOiK09677.
OMAiRGDHYAR.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ96RP7.
TreeFamiTF314802.

Enzyme and pathway databases

UniPathwayiUPA00353.

Miscellaneous databases

GeneWikiiGAL3ST4.
GenomeRNAii79690.
NextBioi35475754.
PROiQ96RP7.
SOURCEiSearch...

Gene expression databases

BgeeiQ96RP7.
CleanExiHS_GAL3ST4.
ExpressionAtlasiQ96RP7. baseline and differential.
GenevisibleiQ96RP7. HS.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-glycans."
    Seko A., Hara-Kuge S., Yamashita K.
    J. Biol. Chem. 276:25697-25704(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-467.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary gland.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Amygdala.
  5. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-467.
    Tissue: Placenta.

Entry informationi

Entry nameiG3ST4_HUMAN
AccessioniPrimary (citable) accession number: Q96RP7
Secondary accession number(s): A4D2A8
, B4DWL8, D6W5U5, Q8N3P7, Q8WZ17, Q96E33, Q9HA78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.