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Q96RP7

- G3ST4_HUMAN

UniProt

Q96RP7 - G3ST4_HUMAN

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Protein

Galactose-3-O-sulfotransferase 4

Gene

GAL3ST4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc.1 Publication

Cofactori

pH dependencei

Optimum pH is 6-7.

Pathwayi

GO - Molecular functioni

  1. 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
  2. galactose 3-O-sulfotransferase activity Source: UniProtKB
  3. galactosylceramide sulfotransferase activity Source: InterPro
  4. proteoglycan sulfotransferase activity Source: UniProtKB

GO - Biological processi

  1. cell-cell signaling Source: UniProtKB
  2. glycoprotein metabolic process Source: UniProtKB
  3. oligosaccharide metabolic process Source: UniProtKB
  4. proteoglycan biosynthetic process Source: UniProtKB
  5. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

UniPathwayiUPA00353.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-3-O-sulfotransferase 4 (EC:2.8.2.-)
Short name:
Gal3ST-4
Alternative name(s):
Beta-galactose-3-O-sulfotransferase 4
Gal-beta-1,3-GalNAc 3'-sulfotransferase
Gene namesi
Name:GAL3ST4
ORF Names:PP6968
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:24145. GAL3ST4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini40 – 486447LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB
  4. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134921067.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Galactose-3-O-sulfotransferase 4PRO_0000085209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ96RP7.
PRIDEiQ96RP7.

PTM databases

PhosphoSiteiQ96RP7.

Expressioni

Tissue specificityi

Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node.1 Publication

Gene expression databases

BgeeiQ96RP7.
CleanExiHS_GAL3ST4.
ExpressionAtlasiQ96RP7. baseline and differential.
GenevestigatoriQ96RP7.

Organism-specific databases

HPAiHPA038137.

Interactioni

Protein-protein interaction databases

BioGridi122811. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ96RP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26344.
GeneTreeiENSGT00390000013505.
HOVERGENiHBG051681.
InParanoidiQ96RP7.
KOiK09677.
OMAiDHYARNL.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ96RP7.
TreeFamiTF314802.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96RP7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ
60 70 80 90 100
PSAPSLRPAL PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP
110 120 130 140 150
ARYQFGYPKL FQASRVKGYR PQGGGTQLPF HILCHHMRFN LKEVLQVMPS
160 170 180 190 200
DSFFFSIVRD PAALARSAFS YYKSTSSAFR KSPSLAAFLA NPRGFYRPGA
210 220 230 240 250
RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL QVLPSGAGPR
260 270 280 290 300
AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL
310 320 330 340 350
VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA
360 370 380 390 400
EDRQLTARAR AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR
410 420 430 440 450
EALAKHCLVG GEASDPKYIT DRRFRPFQFG SAKVLGYILR SGLSPQDQEE
460 470 480
CERLATPELQ YKDKLDAKQF PPTVSLPLKT SRPLSP
Length:486
Mass (Da):54,166
Last modified:December 1, 2001 - v1
Checksum:i2D731D16B191CC3C
GO
Isoform 2 (identifier: Q96RP7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MGPLSPARTL...FLKTHKSGSS → MTPRSPTSAF...TPALGRALPE
     81-142: Missing.

Note: No experimental confirmation available

Show »
Length:424
Mass (Da):46,588
Checksum:i43AFFE8F564E79A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371M → L in BAB13977. (PubMed:14702039)Curated
Sequence conflicti201 – 2044RGDH → VGTT in AAL55759. (PubMed:15498874)Curated
Sequence conflicti441 – 4411S → R in BAB13977. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti353 – 3531R → Q.
Corresponds to variant rs3800952 [ dbSNP | Ensembl ].
VAR_021989
Natural varianti467 – 4671A → V.2 Publications
Corresponds to variant rs3823646 [ dbSNP | Ensembl ].
VAR_033736

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8080MGPLS…KSGSS → MTPRSPTSAFRSCTAVSSCL CPSSWPWLHLSVTSPFPVPH GPSLSCQDAAALGTSEPGGG SGSLHDHWLCTPALGRALPE in isoform 2. 1 PublicationVSP_057006Add
BLAST
Alternative sequencei81 – 14262Missing in isoform 2. 1 PublicationVSP_057007Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316113 mRNA. Translation: AAK73365.1.
AF289575 mRNA. Translation: AAL55759.1.
AK022178 mRNA. Translation: BAB13977.1.
AK301591 mRNA. Translation: BAG63080.1.
AL833824 mRNA. Translation: CAD38686.2.
AC073842 Genomic DNA. No translation available.
CH236956 Genomic DNA. Translation: EAL23847.1.
CH471091 Genomic DNA. Translation: EAW76575.1.
CH471091 Genomic DNA. Translation: EAW76577.1.
CH471091 Genomic DNA. Translation: EAW76579.1.
BC012976 mRNA. Translation: AAH12976.1.
CCDSiCCDS5688.1.
RefSeqiNP_078913.3. NM_024637.4.
UniGeneiHs.44856.

Genome annotation databases

EnsembliENST00000360039; ENSP00000353142; ENSG00000197093. [Q96RP7-1]
ENST00000413800; ENSP00000400451; ENSG00000197093. [Q96RP7-1]
GeneIDi79690.
KEGGihsa:79690.
UCSCiuc003utt.3. human. [Q96RP7-1]

Polymorphism databases

DMDMi47116570.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316113 mRNA. Translation: AAK73365.1 .
AF289575 mRNA. Translation: AAL55759.1 .
AK022178 mRNA. Translation: BAB13977.1 .
AK301591 mRNA. Translation: BAG63080.1 .
AL833824 mRNA. Translation: CAD38686.2 .
AC073842 Genomic DNA. No translation available.
CH236956 Genomic DNA. Translation: EAL23847.1 .
CH471091 Genomic DNA. Translation: EAW76575.1 .
CH471091 Genomic DNA. Translation: EAW76577.1 .
CH471091 Genomic DNA. Translation: EAW76579.1 .
BC012976 mRNA. Translation: AAH12976.1 .
CCDSi CCDS5688.1.
RefSeqi NP_078913.3. NM_024637.4.
UniGenei Hs.44856.

3D structure databases

ProteinModelPortali Q96RP7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122811. 1 interaction.

PTM databases

PhosphoSitei Q96RP7.

Polymorphism databases

DMDMi 47116570.

Proteomic databases

PaxDbi Q96RP7.
PRIDEi Q96RP7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000360039 ; ENSP00000353142 ; ENSG00000197093 . [Q96RP7-1 ]
ENST00000413800 ; ENSP00000400451 ; ENSG00000197093 . [Q96RP7-1 ]
GeneIDi 79690.
KEGGi hsa:79690.
UCSCi uc003utt.3. human. [Q96RP7-1 ]

Organism-specific databases

CTDi 79690.
GeneCardsi GC07M099790.
HGNCi HGNC:24145. GAL3ST4.
HPAi HPA038137.
MIMi 608235. gene.
neXtProti NX_Q96RP7.
PharmGKBi PA134921067.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG26344.
GeneTreei ENSGT00390000013505.
HOVERGENi HBG051681.
InParanoidi Q96RP7.
KOi K09677.
OMAi DHYARNL.
OrthoDBi EOG7K0ZF1.
PhylomeDBi Q96RP7.
TreeFami TF314802.

Enzyme and pathway databases

UniPathwayi UPA00353 .

Miscellaneous databases

GeneWikii GAL3ST4.
GenomeRNAii 79690.
NextBioi 68960.
PROi Q96RP7.
SOURCEi Search...

Gene expression databases

Bgeei Q96RP7.
CleanExi HS_GAL3ST4.
ExpressionAtlasi Q96RP7. baseline and differential.
Genevestigatori Q96RP7.

Family and domain databases

InterProi IPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR14647. PTHR14647. 1 hit.
Pfami PF06990. Gal-3-0_sulfotr. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-glycans."
    Seko A., Hara-Kuge S., Yamashita K.
    J. Biol. Chem. 276:25697-25704(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-467.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary gland.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Amygdala.
  5. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-467.
    Tissue: Placenta.

Entry informationi

Entry nameiG3ST4_HUMAN
AccessioniPrimary (citable) accession number: Q96RP7
Secondary accession number(s): A4D2A8
, B4DWL8, D6W5U5, Q8N3P7, Q8WZ17, Q96E33, Q9HA78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3