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Protein

Galactose-3-O-sulfotransferase 4

Gene

GAL3ST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc.1 Publication

Cofactori

pH dependencei

Optimum pH is 6-7.

Pathwayi: carbohydrate sulfation

This protein is involved in the pathway carbohydrate sulfation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway carbohydrate sulfation and in Protein modification.

GO - Molecular functioni

  • 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
  • galactose 3-O-sulfotransferase activity Source: UniProtKB
  • galactosylceramide sulfotransferase activity Source: InterPro
  • proteoglycan sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • cell-cell signaling Source: UniProtKB
  • glycolipid biosynthetic process Source: InterPro
  • glycoprotein metabolic process Source: UniProtKB
  • oligosaccharide metabolic process Source: UniProtKB
  • proteoglycan biosynthetic process Source: UniProtKB
  • sulfur compound metabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

UniPathwayiUPA00353

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-3-O-sulfotransferase 4 (EC:2.8.2.-)
Short name:
Gal3ST-4
Alternative name(s):
Beta-galactose-3-O-sulfotransferase 4
Gal-beta-1,3-GalNAc 3'-sulfotransferase
Gene namesi
Name:GAL3ST4
ORF Names:PP6968
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000197093.10
HGNCiHGNC:24145 GAL3ST4
MIMi608235 gene
neXtProtiNX_Q96RP7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini40 – 486LumenalSequence analysisAdd BLAST447

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi79690
OpenTargetsiENSG00000197093
PharmGKBiPA134921067

Polymorphism and mutation databases

BioMutaiGAL3ST4
DMDMi47116570

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852091 – 486Galactose-3-O-sulfotransferase 4Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ96RP7
PeptideAtlasiQ96RP7
PRIDEiQ96RP7
ProteomicsDBi77998

PTM databases

iPTMnetiQ96RP7
PhosphoSitePlusiQ96RP7

Expressioni

Tissue specificityi

Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node.1 Publication

Gene expression databases

BgeeiENSG00000197093
CleanExiHS_GAL3ST4
ExpressionAtlasiQ96RP7 baseline and differential
GenevisibleiQ96RP7 HS

Organism-specific databases

HPAiHPA038137
HPA038138

Interactioni

Protein-protein interaction databases

BioGridi122811, 1 interactor
IntActiQ96RP7, 1 interactor
STRINGi9606.ENSP00000353142

Structurei

3D structure databases

ProteinModelPortaliQ96RP7
SMRiQ96RP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG5S Eukaryota
ENOG4110RXY LUCA
GeneTreeiENSGT00390000013505
HOGENOMiHOG000232148
HOVERGENiHBG051681
InParanoidiQ96RP7
KOiK09677
OMAiDSFFFSI
OrthoDBiEOG091G0N28
PhylomeDBiQ96RP7
TreeFamiTF314802

Family and domain databases

InterProiView protein in InterPro
IPR009729 Gal-3-0_sulfotransfrase
IPR027417 P-loop_NTPase
PANTHERiPTHR14647 PTHR14647, 1 hit
PfamiView protein in Pfam
PF06990 Gal-3-0_sulfotr, 2 hits
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96RP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ
60 70 80 90 100
PSAPSLRPAL PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP
110 120 130 140 150
ARYQFGYPKL FQASRVKGYR PQGGGTQLPF HILCHHMRFN LKEVLQVMPS
160 170 180 190 200
DSFFFSIVRD PAALARSAFS YYKSTSSAFR KSPSLAAFLA NPRGFYRPGA
210 220 230 240 250
RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL QVLPSGAGPR
260 270 280 290 300
AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL
310 320 330 340 350
VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA
360 370 380 390 400
EDRQLTARAR AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR
410 420 430 440 450
EALAKHCLVG GEASDPKYIT DRRFRPFQFG SAKVLGYILR SGLSPQDQEE
460 470 480
CERLATPELQ YKDKLDAKQF PPTVSLPLKT SRPLSP
Length:486
Mass (Da):54,166
Last modified:December 1, 2001 - v1
Checksum:i2D731D16B191CC3C
GO
Isoform 2 (identifier: Q96RP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MGPLSPARTL...FLKTHKSGSS → MTPRSPTSAF...TPALGRALPE
     81-142: Missing.

Note: No experimental confirmation available.
Show »
Length:424
Mass (Da):46,588
Checksum:i43AFFE8F564E79A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137M → L in BAB13977 (PubMed:14702039).Curated1
Sequence conflicti201 – 204RGDH → VGTT in AAL55759 (PubMed:15498874).Curated4
Sequence conflicti441S → R in BAB13977 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021989353R → Q. Corresponds to variant dbSNP:rs3800952Ensembl.1
Natural variantiVAR_033736467A → V2 PublicationsCorresponds to variant dbSNP:rs3823646Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0570061 – 80MGPLS…KSGSS → MTPRSPTSAFRSCTAVSSCL CPSSWPWLHLSVTSPFPVPH GPSLSCQDAAALGTSEPGGG SGSLHDHWLCTPALGRALPE in isoform 2. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_05700781 – 142Missing in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316113 mRNA Translation: AAK73365.1
AF289575 mRNA Translation: AAL55759.1
AK022178 mRNA Translation: BAB13977.1
AK301591 mRNA Translation: BAG63080.1
AL833824 mRNA Translation: CAD38686.2
AC073842 Genomic DNA No translation available.
CH236956 Genomic DNA Translation: EAL23847.1
CH471091 Genomic DNA Translation: EAW76575.1
CH471091 Genomic DNA Translation: EAW76577.1
CH471091 Genomic DNA Translation: EAW76579.1
BC012976 mRNA Translation: AAH12976.1
CCDSiCCDS5688.1 [Q96RP7-1]
RefSeqiNP_078913.3, NM_024637.4 [Q96RP7-1]
UniGeneiHs.44856

Genome annotation databases

EnsembliENST00000360039; ENSP00000353142; ENSG00000197093 [Q96RP7-1]
ENST00000413800; ENSP00000400451; ENSG00000197093 [Q96RP7-1]
GeneIDi79690
KEGGihsa:79690
UCSCiuc003utt.4 human [Q96RP7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiG3ST4_HUMAN
AccessioniPrimary (citable) accession number: Q96RP7
Secondary accession number(s): A4D2A8
, B4DWL8, D6W5U5, Q8N3P7, Q8WZ17, Q96E33, Q9HA78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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