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Q96RP7 (G3ST4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactose-3-O-sulfotransferase 4

Short name=Gal3ST-4
EC=2.8.2.-
Alternative name(s):
Beta-galactose-3-O-sulfotransferase 4
Gal-beta-1,3-GalNAc 3'-sulfotransferase
Gene names
Name:GAL3ST4
ORF Names:PP6968
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc. Ref.1

Cofactor

Manganese.

Pathway

Protein modification; carbohydrate sulfation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein Probable.

Tissue specificity

Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node. Ref.1

Sequence similarities

Belongs to the galactose-3-O-sulfotransferase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 6-7.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell-cell signaling

Non-traceable author statement Ref.1. Source: UniProtKB

glycoprotein metabolic process

Non-traceable author statement Ref.1. Source: UniProtKB

oligosaccharide metabolic process

Non-traceable author statement Ref.1. Source: UniProtKB

proteoglycan biosynthetic process

Non-traceable author statement Ref.1. Source: UniProtKB

sulfur compound metabolic process

Non-traceable author statement Ref.1. Source: UniProtKB

   Cellular_componentGolgi cisterna membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular vesicular exosome

Inferred from direct assay PubMed 19199708. Source: UniProt

integral component of membrane

Non-traceable author statement Ref.1. Source: UniProtKB

membrane

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_function3'-phosphoadenosine 5'-phosphosulfate binding

Non-traceable author statement Ref.1. Source: UniProtKB

galactose 3-O-sulfotransferase activity

Inferred from direct assay Ref.1. Source: UniProtKB

galactosylceramide sulfotransferase activity

Inferred from electronic annotation. Source: InterPro

proteoglycan sulfotransferase activity

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Galactose-3-O-sulfotransferase 4
PRO_0000085209

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3921Helical; Signal-anchor for type II membrane protein; Potential
Topological domain40 – 486447Lumenal Potential

Amino acid modifications

Glycosylation3741N-linked (GlcNAc...) Potential

Natural variations

Natural variant3531R → Q.
Corresponds to variant rs3800952 [ dbSNP | Ensembl ].
VAR_021989
Natural variant4671A → V. Ref.2 Ref.7
Corresponds to variant rs3823646 [ dbSNP | Ensembl ].
VAR_033736

Experimental info

Sequence conflict1371M → L in BAB13977. Ref.3
Sequence conflict201 – 2044RGDH → VGTT in AAL55759. Ref.2
Sequence conflict4411S → R in BAB13977. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q96RP7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 2D731D16B191CC3C

FASTA48654,166
        10         20         30         40         50         60 
MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ PSAPSLRPAL 

        70         80         90        100        110        120 
PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP ARYQFGYPKL FQASRVKGYR 

       130        140        150        160        170        180 
PQGGGTQLPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR 

       190        200        210        220        230        240 
KSPSLAAFLA NPRGFYRPGA RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL 

       250        260        270        280        290        300 
QVLPSGAGPR AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL 

       310        320        330        340        350        360 
VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA EDRQLTARAR 

       370        380        390        400        410        420 
AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR EALAKHCLVG GEASDPKYIT 

       430        440        450        460        470        480 
DRRFRPFQFG SAKVLGYILR SGLSPQDQEE CERLATPELQ YKDKLDAKQF PPTVSLPLKT 


SRPLSP 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-glycans."
Seko A., Hara-Kuge S., Yamashita K.
J. Biol. Chem. 276:25697-25704(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-467.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[5]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-467.
Tissue: Placenta.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF316113 mRNA. Translation: AAK73365.1.
AF289575 mRNA. Translation: AAL55759.1.
AK022178 mRNA. Translation: BAB13977.1.
AL833824 mRNA. Translation: CAD38686.2.
CH236956 Genomic DNA. Translation: EAL23847.1.
CH471091 Genomic DNA. Translation: EAW76575.1.
CH471091 Genomic DNA. Translation: EAW76577.1.
CH471091 Genomic DNA. Translation: EAW76579.1.
BC012976 mRNA. Translation: AAH12976.1.
CCDSCCDS5688.1.
RefSeqNP_078913.3. NM_024637.4.
UniGeneHs.44856.

3D structure databases

ProteinModelPortalQ96RP7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122811. 1 interaction.

PTM databases

PhosphoSiteQ96RP7.

Polymorphism databases

DMDM47116570.

Proteomic databases

PaxDbQ96RP7.
PRIDEQ96RP7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360039; ENSP00000353142; ENSG00000197093.
ENST00000413800; ENSP00000400451; ENSG00000197093.
GeneID79690.
KEGGhsa:79690.
UCSCuc003utt.3. human.

Organism-specific databases

CTD79690.
GeneCardsGC07M099770.
HGNCHGNC:24145. GAL3ST4.
HPAHPA038137.
MIM608235. gene.
neXtProtNX_Q96RP7.
PharmGKBPA134921067.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG26344.
HOVERGENHBG051681.
InParanoidQ96RP7.
KOK09677.
OMADHYARNL.
OrthoDBEOG7K0ZF1.
PhylomeDBQ96RP7.
TreeFamTF314802.

Enzyme and pathway databases

UniPathwayUPA00353.

Gene expression databases

ArrayExpressQ96RP7.
BgeeQ96RP7.
CleanExHS_GAL3ST4.
GenevestigatorQ96RP7.

Family and domain databases

InterProIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR14647. PTHR14647. 1 hit.
PfamPF06990. Gal-3-0_sulfotr. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

GeneWikiGAL3ST4.
GenomeRNAi79690.
NextBio68960.
PROQ96RP7.
SOURCESearch...

Entry information

Entry nameG3ST4_HUMAN
AccessionPrimary (citable) accession number: Q96RP7
Secondary accession number(s): A4D2A8 expand/collapse secondary AC list , D6W5U5, Q8N3P7, Q8WZ17, Q96E33, Q9HA78
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM