Q96RL6 (SIG11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialic acid-binding Ig-like lectin 11
Short name=Sialic acid-binding lectin 11
Short name=Siglec-11

Gene names

Name:	SIGLEC11
ORF Names:	UNQ9222/PRO28718

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
Subunit structure	Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Ref.1
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed by macrophages in various tissues including Kupffer cells. Also found in brain microglia.
Domain	Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Post-translational modification	Phosphorylated on tyrosine residues.
Sequence similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Caution	It is uncertain whether Met-1 or Met-13 is the initiator.
Sequence caution	The sequence AAK72907.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAQ88502.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96RL6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96RL6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     455-551: YPPQLLGPSC...SGSVFQLLPG → W

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 698	671	Sialic acid-binding Ig-like lectin 11		PRO_0000014951
Regions
	Topological domain	28 – 561	534	Extracellular Potential
	Transmembrane	562 – 584	23	Helical; Potential
	Topological domain	585 – 698	114	Cytoplasmic Potential
	Domain	31 – 134	104	Ig-like V-type
	Domain	159 – 244	86	Ig-like C2-type 1
	Domain	251 – 350	100	Ig-like C2-type 2
	Domain	355 – 452	98	Ig-like C2-type 3
	Motif	642 – 647	6	ITIM motif
Sites
	Binding site	132	1	Sialic acid By similarity
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Glycosylation	515	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 186		By similarity
	Disulfide bond	54 ↔ 114		By similarity
	Disulfide bond	177 ↔ 228		By similarity
	Disulfide bond	287 ↔ 334		By similarity
	Disulfide bond	391 ↔ 436		By similarity
Natural variations
	Alternative sequence	455 – 551	97	YPPQL…QLLPG → W in isoform 2.		VSP_008764
Experimental info
	Sequence conflict	96	1	E → A in AAQ88502. Ref.3
	Sequence conflict	365	1	A → G in AAQ88502. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 61CF0F530DCDD62F	FASTA	698	75,795
	10 20 30 40 50 60 MVPGQAQPQS PEMLLLPLLL PVLGAGSLNK DPSYSLQVQR QVPVPEGLCV IVSCNLSYPR 70 80 90 100 110 120 DGWDESTAAY GYWFKGRTSP KTGAPVATNN QSREVEMSTR DRFQLTGDPG KGSCSLVIRD 130 140 150 160 170 180 AQREDEAWYF FRVERGSRVR HSFLSNAFFL KVTALTKKPD VYIPETLEPG QPVTVICVFN 190 200 210 220 230 240 WAFKKCPAPS FSWTGAALSP RRTRPSTSHF SVLSFTPSPQ DHDTDLTCHV DFSRKGVSAQ 250 260 270 280 290 300 RTVRLRVAYA PKDLIISISH DNTSALELQG NVIYLEVQKG QFLRLLCAAD SQPPATLSWV 310 320 330 340 350 360 LQDRVLSSSH PWGPRTLGLE LRGVRAGDSG RYTCRAENRL GSQQQALDLS VQYPPENLRV 370 380 390 400 410 420 MVSQANRTVL ENLGNGTSLP VLEGQSLRLV CVTHSSPPAR LSWTRWGQTV GPSQPSDPGV 430 440 450 460 470 480 LELPPIQMEH EGEFTCHAQH PLGSQHVSLS LSVHYPPQLL GPSCSWEAEG LHCSCSSQAS 490 500 510 520 530 540 PAPSLRWWLG EELLEGNSSQ GSFEVTPSSA GPWANSSLSL HGGLSSGLRL RCKAWNVHGA 550 560 570 580 590 600 QSGSVFQLLP GKLEHGGGLG LGAALGAGVA ALLAFCSCLV VFRVKICRKE ARKRAAAEQD 610 620 630 640 650 660 VPSTLGPISQ GHQHECSAGS SQDHPPPGAA TYTPGKGEEQ ELHYASLSFQ GLRLWEPADQ 670 680 690 EAPSTTEYSE IKIHTGQPLR GPGFGLQLER EMSGMVPK
	Isoform 2 [UniParc]. Checksum: 648623DDB469EA95	FASTA	602	65,818
	10 20 30 40 50 60 MVPGQAQPQS PEMLLLPLLL PVLGAGSLNK DPSYSLQVQR QVPVPEGLCV IVSCNLSYPR 70 80 90 100 110 120 DGWDESTAAY GYWFKGRTSP KTGAPVATNN QSREVEMSTR DRFQLTGDPG KGSCSLVIRD 130 140 150 160 170 180 AQREDEAWYF FRVERGSRVR HSFLSNAFFL KVTALTKKPD VYIPETLEPG QPVTVICVFN 190 200 210 220 230 240 WAFKKCPAPS FSWTGAALSP RRTRPSTSHF SVLSFTPSPQ DHDTDLTCHV DFSRKGVSAQ 250 260 270 280 290 300 RTVRLRVAYA PKDLIISISH DNTSALELQG NVIYLEVQKG QFLRLLCAAD SQPPATLSWV 310 320 330 340 350 360 LQDRVLSSSH PWGPRTLGLE LRGVRAGDSG RYTCRAENRL GSQQQALDLS VQYPPENLRV 370 380 390 400 410 420 MVSQANRTVL ENLGNGTSLP VLEGQSLRLV CVTHSSPPAR LSWTRWGQTV GPSQPSDPGV 430 440 450 460 470 480 LELPPIQMEH EGEFTCHAQH PLGSQHVSLS LSVHWKLEHG GGLGLGAALG AGVAALLAFC 490 500 510 520 530 540 SCLVVFRVKI CRKEARKRAA AEQDVPSTLG PISQGHQHEC SAGSSQDHPP PGAATYTPGK 550 560 570 580 590 600 GEEQELHYAS LSFQGLRLWE PADQEAPSTT EYSEIKIHTG QPLRGPGFGL QLEREMSGMV PK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of human Siglec-11. A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia." Angata T., Kerr S.C., Greaves D.R., Varki N.M., Crocker P.R., Varki A. J. Biol. Chem. 277:24466-24474(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH PTPN6 AND PTPN11.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF337818 mRNA. Translation: AAK72907.1. Different initiation. AC011452 Genomic DNA. No translation available. AY358135 mRNA. Translation: AAQ88502.1. Different initiation.
IPI	IPI00385641. IPI00744876.
RefSeq	NP_001128635.1. NM_001135163.1. NP_443116.2. NM_052884.2.
UniGene	Hs.661852.
3D structure databases
ProteinModelPortal	Q96RL6.
SMR	Q96RL6. Positions 34-546.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96RL6. 5 interactions.
STRING	9606.ENSP00000412361.
Protein family/group databases
MEROPS	I43.001.
PTM databases
PhosphoSite	Q96RL6.
Polymorphism databases
DMDM	294862467.
Proteomic databases
PaxDb	Q96RL6.
PRIDE	Q96RL6.
Protocols and materials databases
DNASU	114132.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000426971; ENSP00000398891; ENSG00000161640. ENST00000447370; ENSP00000412361; ENSG00000161640.
GeneID	114132.
KEGG	hsa:114132.
UCSC	uc010ybh.2. human. uc010ybi.2. human.
Organism-specific databases
CTD	114132.
GeneCards	GC19M050412.
HGNC	HGNC:15622. SIGLEC11.
MIM	607157. gene.
neXtProt	NX_Q96RL6.
PharmGKB	PA38005.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG280582.
HOGENOM	HOG000236324.
HOVERGEN	HBG036161.
InParanoid	Q96RL6.
KO	K06750.
OMA	LGPISQG.
OrthoDB	EOG43BMP7.
Gene expression databases
ArrayExpress	Q96RL6.
Bgee	Q96RL6.
CleanEx	HS_SIGLEC11.
Genevestigator	Q96RL6.
GermOnline	ENSG00000161640. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 4 hits.
InterPro	IPR013162. CD80_C2-set. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. IPR013151. Immunoglobulin. [Graphical view]
Pfam	PF08205. C2-set_2. 1 hit. PF07679. I-set. 1 hit. PF00047. ig. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	114132.
NextBio	79001.
SOURCE	Search...

Entry information

Entry name

SIG11_HUMAN

Accession

Primary (citable) accession number: Q96RL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.