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Q96RI0

- PAR4_HUMAN

UniProt

Q96RI0 - PAR4_HUMAN

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Protein

Proteinase-activated receptor 4

Gene

F2RL3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei47 – 482Cleavage; by thrombin or trypsinBy similarity

GO - Molecular functioni

  1. thrombin receptor activity Source: ProtInc

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. phospholipase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
  3. platelet activation Source: Reactome
  4. platelet dense granule organization Source: BHF-UCL
  5. positive regulation of release of sequestered calcium ion into cytosol Source: BHF-UCL
  6. response to wounding Source: ProtInc
  7. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.
REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 4
Short name:
PAR-4
Alternative name(s):
Coagulation factor II receptor-like 3
Thrombin receptor-like 3
Gene namesi
Name:F2RL3
Synonyms:PAR4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:3540. F2RL3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini48 – 8235ExtracellularSequence AnalysisAdd
BLAST
Transmembranei83 – 10321Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini104 – 1085CytoplasmicSequence Analysis
Transmembranei109 – 12921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini130 – 15122ExtracellularSequence AnalysisAdd
BLAST
Transmembranei152 – 17221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini173 – 19220CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei193 – 21321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini214 – 24734ExtracellularSequence AnalysisAdd
BLAST
Transmembranei248 – 26821Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini269 – 28315CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei284 – 30421Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini305 – 31915ExtracellularSequence AnalysisAdd
BLAST
Transmembranei320 – 34324Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini344 – 38542CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. integral component of plasma membrane Source: ProtInc
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471R → A: No proteolytic cleavage (by thrombin or trypsin). 1 Publication
Mutagenesisi68 – 681R → A: No effect on receptor activation. 1 Publication

Organism-specific databases

PharmGKBiPA27949.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Propeptidei18 – 4730Removed for receptor activationBy similarityPRO_0000012762Add
BLAST
Chaini48 – 385338Proteinase-activated receptor 4PRO_0000012763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi149 ↔ 228PROSITE-ProRule annotation

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96RI0.
PRIDEiQ96RI0.

PTM databases

PhosphoSiteiQ96RI0.

Miscellaneous databases

PMAP-CutDBQ96RI0.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.

Gene expression databases

BgeeiQ96RI0.
CleanExiHS_F2RL3.
ExpressionAtlasiQ96RI0. baseline.
GenevestigatoriQ96RI0.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000248076.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi44 – 463Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZPKX-ray1.80P/Q46-53[»]
3QDZX-ray2.80E/F39-47[»]
ProteinModelPortaliQ96RI0.
SMRiQ96RI0. Positions 51-348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96RI0.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi301 – 3055Poly-Leu

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148814.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000116291.
HOVERGENiHBG096365.
InParanoidiQ96RI0.
KOiK04236.
OMAiYYVSAEF.
OrthoDBiEOG79PJPM.
PhylomeDBiQ96RI0.
TreeFamiTF350010.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003944. Prot_act_rcpt_4.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01430. PROTEASEAR4.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96RI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWGRLLLWPL VLGFSLSGGT QTPSVYDESG STGGGDDSTP SILPAPRGYP
60 70 80 90 100
GQVCANDSDT LELPDSSRAL LLGWVPTRLV PALYGLVLVV GLPANGLALW
110 120 130 140 150
VLATQAPRLP STMLLMNLAA ADLLLALALP PRIAYHLRGQ RWPFGEAACR
160 170 180 190 200
LATAALYGHM YGSVLLLAAV SLDRYLALVH PLRARALRGR RLALGLCMAA
210 220 230 240 250
WLMAAALALP LTLQRQTFRL ARSDRVLCHD ALPLDAQASH WQPAFTCLAL
260 270 280 290 300
LGCFLPLLAM LLCYGATLHT LAASGRRYGH ALRLTAVVLA SAVAFFVPSN
310 320 330 340 350
LLLLLHYSDP SPSAWGNLYG AYVPSLALST LNSCVDPFIY YYVSAEFRDK
360 370 380
VRAGLFQRSP GDTVASKASA EGGSRGMGTH SSLLQ
Length:385
Mass (Da):41,133
Last modified:October 17, 2006 - v3
Checksum:iFACE32F10D5C561E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti120 – 1201A → T.3 Publications
Corresponds to variant rs773902 [ dbSNP | Ensembl ].
VAR_028300
Natural varianti215 – 2151R → Q.
Corresponds to variant rs2230799 [ dbSNP | Ensembl ].
VAR_028301
Natural varianti296 – 2961F → V.1 Publication
Corresponds to variant rs2227346 [ dbSNP | Ensembl ].
VAR_012852
Natural varianti310 – 3101P → L.1 Publication
Corresponds to variant rs2227376 [ dbSNP | Ensembl ].
VAR_012853

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080214 mRNA. Translation: AAC28860.1.
AF055917 mRNA. Translation: AAC25699.1.
AY431102 mRNA. Translation: AAR08487.1.
AF384819 Genomic DNA. Translation: AAK61908.1.
BC074782 mRNA. Translation: AAH74782.2.
CCDSiCCDS12350.1.
RefSeqiNP_003941.2. NM_003950.2.
UniGeneiHs.137574.

Genome annotation databases

EnsembliENST00000248076; ENSP00000248076; ENSG00000127533.
GeneIDi9002.
KEGGihsa:9002.
UCSCiuc002nfa.3. human.

Polymorphism databases

DMDMi116242700.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080214 mRNA. Translation: AAC28860.1 .
AF055917 mRNA. Translation: AAC25699.1 .
AY431102 mRNA. Translation: AAR08487.1 .
AF384819 Genomic DNA. Translation: AAK61908.1 .
BC074782 mRNA. Translation: AAH74782.2 .
CCDSi CCDS12350.1.
RefSeqi NP_003941.2. NM_003950.2.
UniGenei Hs.137574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZPK X-ray 1.80 P/Q 46-53 [» ]
3QDZ X-ray 2.80 E/F 39-47 [» ]
ProteinModelPortali Q96RI0.
SMRi Q96RI0. Positions 51-348.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000248076.

Chemistry

ChEMBLi CHEMBL4691.
GuidetoPHARMACOLOGYi 350.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q96RI0.

Polymorphism databases

DMDMi 116242700.

Proteomic databases

PaxDbi Q96RI0.
PRIDEi Q96RI0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000248076 ; ENSP00000248076 ; ENSG00000127533 .
GeneIDi 9002.
KEGGi hsa:9002.
UCSCi uc002nfa.3. human.

Organism-specific databases

CTDi 9002.
GeneCardsi GC19P017898.
HGNCi HGNC:3540. F2RL3.
MIMi 602779. gene.
neXtProti NX_Q96RI0.
PharmGKBi PA27949.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG148814.
GeneTreei ENSGT00760000119001.
HOGENOMi HOG000116291.
HOVERGENi HBG096365.
InParanoidi Q96RI0.
KOi K04236.
OMAi YYVSAEF.
OrthoDBi EOG79PJPM.
PhylomeDBi Q96RI0.
TreeFami TF350010.

Enzyme and pathway databases

Reactomei REACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.
REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

Miscellaneous databases

EvolutionaryTracei Q96RI0.
GeneWikii F2RL3.
GenomeRNAii 9002.
NextBioi 33761.
PMAP-CutDB Q96RI0.
PROi Q96RI0.
SOURCEi Search...

Gene expression databases

Bgeei Q96RI0.
CleanExi HS_F2RL3.
ExpressionAtlasi Q96RI0. baseline.
Genevestigatori Q96RI0.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003944. Prot_act_rcpt_4.
IPR003912. Protea_act_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01430. PROTEASEAR4.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene and locus structure and chromosomal localization of the protease-activated receptor gene family."
    Kahn M.L., Hammes S.R., Botka C., Coughlin S.R.
    J. Biol. Chem. 273:23290-23296(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-47 AND ARG-68, VARIANT THR-120.
    Tissue: Lymphoma.
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    King M.M., Aronstam R.S., Sharma S.V.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. SeattleSNPs variation discovery resource
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-296 AND LEU-310.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin."
    Kahn M.L., Nakanishi-Matsui M., Shapiro M.J., Ishihara H., Coughlin S.R.
    J. Clin. Invest. 103:879-887(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPAR4_HUMAN
AccessioniPrimary (citable) accession number: Q96RI0
Secondary accession number(s): O76067, Q6DK42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 17, 2006
Last modified: November 26, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3