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Q96RI0 (PAR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteinase-activated receptor 4

Short name=PAR-4
Alternative name(s):
Coagulation factor II receptor-like 3
Thrombin receptor-like 3
Gene names
Name:F2RL3
Synonyms:PAR4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.

Post-translational modification

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 4730Removed for receptor activation By similarity
PRO_0000012762
Chain48 – 385338Proteinase-activated receptor 4
PRO_0000012763

Regions

Topological domain48 – 8235Extracellular Potential
Transmembrane83 – 10321Helical; Name=1; Potential
Topological domain104 – 1085Cytoplasmic Potential
Transmembrane109 – 12921Helical; Name=2; Potential
Topological domain130 – 15122Extracellular Potential
Transmembrane152 – 17221Helical; Name=3; Potential
Topological domain173 – 19220Cytoplasmic Potential
Transmembrane193 – 21321Helical; Name=4; Potential
Topological domain214 – 24734Extracellular Potential
Transmembrane248 – 26821Helical; Name=5; Potential
Topological domain269 – 28315Cytoplasmic Potential
Transmembrane284 – 30421Helical; Name=6; Potential
Topological domain305 – 31915Extracellular Potential
Transmembrane320 – 34324Helical; Name=7; Potential
Topological domain344 – 38542Cytoplasmic Potential
Compositional bias301 – 3055Poly-Leu

Sites

Site47 – 482Cleavage; by thrombin or trypsin By similarity

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential
Disulfide bond149 ↔ 228 By similarity

Natural variations

Natural variant1201A → T. Ref.1 Ref.2 Ref.3
Corresponds to variant rs773902 [ dbSNP | Ensembl ].
VAR_028300
Natural variant2151R → Q.
Corresponds to variant rs2230799 [ dbSNP | Ensembl ].
VAR_028301
Natural variant2961F → V. Ref.5
Corresponds to variant rs2227346 [ dbSNP | Ensembl ].
VAR_012852
Natural variant3101P → L. Ref.5
Corresponds to variant rs2227376 [ dbSNP | Ensembl ].
VAR_012853

Experimental info

Mutagenesis471R → A: No proteolytic cleavage (by thrombin or trypsin). Ref.3
Mutagenesis681R → A: No effect on receptor activation. Ref.3

Secondary structure

... 385
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q96RI0 [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: FACE32F10D5C561E

FASTA38541,133
        10         20         30         40         50         60 
MWGRLLLWPL VLGFSLSGGT QTPSVYDESG STGGGDDSTP SILPAPRGYP GQVCANDSDT 

        70         80         90        100        110        120 
LELPDSSRAL LLGWVPTRLV PALYGLVLVV GLPANGLALW VLATQAPRLP STMLLMNLAA 

       130        140        150        160        170        180 
ADLLLALALP PRIAYHLRGQ RWPFGEAACR LATAALYGHM YGSVLLLAAV SLDRYLALVH 

       190        200        210        220        230        240 
PLRARALRGR RLALGLCMAA WLMAAALALP LTLQRQTFRL ARSDRVLCHD ALPLDAQASH 

       250        260        270        280        290        300 
WQPAFTCLAL LGCFLPLLAM LLCYGATLHT LAASGRRYGH ALRLTAVVLA SAVAFFVPSN 

       310        320        330        340        350        360 
LLLLLHYSDP SPSAWGNLYG AYVPSLALST LNSCVDPFIY YYVSAEFRDK VRAGLFQRSP 

       370        380 
GDTVASKASA EGGSRGMGTH SSLLQ 

« Hide

References

« Hide 'large scale' references
[1]"Gene and locus structure and chromosomal localization of the protease-activated receptor gene family."
Kahn M.L., Hammes S.R., Botka C., Coughlin S.R.
J. Biol. Chem. 273:23290-23296(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
[2]"A dual thrombin receptor system for platelet activation."
Kahn M.L., Zheng Y.-W., Huang W., Bigornia V., Zeng D., Moff S., Farese R.V. Jr., Tam C., Coughlin S.R.
Nature 394:690-694(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
[3]"Cloning and characterization of human protease-activated receptor 4."
Xu W.-F., Andersen H., Whitmore T.E., Presnell S.R., Yee D.P., Ching A., Gilbert T., Davie E.W., Foster D.C.
Proc. Natl. Acad. Sci. U.S.A. 95:6642-6646(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-47 AND ARG-68, VARIANT THR-120.
Tissue: Lymphoma.
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
King M.M., Aronstam R.S., Sharma S.V.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]SeattleSNPs variation discovery resource
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-296 AND LEU-310.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin."
Kahn M.L., Nakanishi-Matsui M., Shapiro M.J., Ishihara H., Coughlin S.R.
J. Clin. Invest. 103:879-887(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF080214 mRNA. Translation: AAC28860.1.
AF055917 mRNA. Translation: AAC25699.1.
AY431102 mRNA. Translation: AAR08487.1.
AF384819 Genomic DNA. Translation: AAK61908.1.
BC074782 mRNA. Translation: AAH74782.2.
RefSeqNP_003941.2. NM_003950.2.
UniGeneHs.137574.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZPKX-ray1.80P/Q46-53[»]
3QDZX-ray2.80E/F39-47[»]
ProteinModelPortalQ96RI0.
SMRQ96RI0. Positions 78-358.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000248076.

Chemistry

BindingDBQ96RI0.
ChEMBLCHEMBL4691.
GuidetoPHARMACOLOGY350.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ96RI0.

Polymorphism databases

DMDM116242700.

Proteomic databases

PaxDbQ96RI0.
PRIDEQ96RI0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000248076; ENSP00000248076; ENSG00000127533.
GeneID9002.
KEGGhsa:9002.
UCSCuc002nfa.3. human.

Organism-specific databases

CTD9002.
GeneCardsGC19P017365.
HGNCHGNC:3540. F2RL3.
MIM602779. gene.
neXtProtNX_Q96RI0.
PharmGKBPA27949.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148814.
HOGENOMHOG000116291.
HOVERGENHBG096365.
InParanoidQ96RI0.
KOK04236.
OMAYYVSAEF.
OrthoDBEOG79PJPM.
PhylomeDBQ96RI0.
TreeFamTF350010.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

BgeeQ96RI0.
CleanExHS_F2RL3.
GenevestigatorQ96RI0.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003944. Prot_act_rcpt_4.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01430. PROTEASEAR4.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ96RI0.
GeneWikiF2RL3.
GenomeRNAi9002.
NextBio33761.
PMAP-CutDBQ96RI0.
PROQ96RI0.
SOURCESearch...

Entry information

Entry namePAR4_HUMAN
AccessionPrimary (citable) accession number: Q96RI0
Secondary accession number(s): O76067, Q6DK42
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries