Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q96RI0

- PAR4_HUMAN

UniProt

Q96RI0 - PAR4_HUMAN

Protein

Proteinase-activated receptor 4

Gene

F2RL3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (17 Oct 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei47 – 482Cleavage; by thrombin or trypsinBy similarity

    GO - Molecular functioni

    1. thrombin receptor activity Source: ProtInc

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. phospholipase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
    3. platelet activation Source: Reactome
    4. platelet dense granule organization Source: BHF-UCL
    5. positive regulation of release of sequestered calcium ion into cytosol Source: BHF-UCL
    6. response to wounding Source: ProtInc
    7. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteinase-activated receptor 4
    Short name:
    PAR-4
    Alternative name(s):
    Coagulation factor II receptor-like 3
    Thrombin receptor-like 3
    Gene namesi
    Name:F2RL3
    Synonyms:PAR4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:3540. F2RL3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi47 – 471R → A: No proteolytic cleavage (by thrombin or trypsin). 1 Publication
    Mutagenesisi68 – 681R → A: No effect on receptor activation. 1 Publication

    Organism-specific databases

    PharmGKBiPA27949.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 4730Removed for receptor activationBy similarityPRO_0000012762Add
    BLAST
    Chaini48 – 385338Proteinase-activated receptor 4PRO_0000012763Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi149 ↔ 228PROSITE-ProRule annotation

    Post-translational modificationi

    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ96RI0.
    PRIDEiQ96RI0.

    PTM databases

    PhosphoSiteiQ96RI0.

    Miscellaneous databases

    PMAP-CutDBQ96RI0.

    Expressioni

    Tissue specificityi

    Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.

    Gene expression databases

    BgeeiQ96RI0.
    CleanExiHS_F2RL3.
    GenevestigatoriQ96RI0.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000248076.

    Structurei

    Secondary structure

    1
    385
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi44 – 463

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZPKX-ray1.80P/Q46-53[»]
    3QDZX-ray2.80E/F39-47[»]
    ProteinModelPortaliQ96RI0.
    SMRiQ96RI0. Positions 51-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96RI0.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini48 – 8235ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini104 – 1085CytoplasmicSequence Analysis
    Topological domaini130 – 15122ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini173 – 19220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini214 – 24734ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini269 – 28315CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini305 – 31915ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini344 – 38542CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei83 – 10321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei152 – 17221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei193 – 21321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei248 – 26821Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei284 – 30421Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei320 – 34324Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi301 – 3055Poly-Leu

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148814.
    HOGENOMiHOG000116291.
    HOVERGENiHBG096365.
    InParanoidiQ96RI0.
    KOiK04236.
    OMAiYYVSAEF.
    OrthoDBiEOG79PJPM.
    PhylomeDBiQ96RI0.
    TreeFamiTF350010.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003944. Prot_act_rcpt_4.
    IPR003912. Protea_act_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR01428. PROTEASEAR.
    PR01430. PROTEASEAR4.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96RI0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWGRLLLWPL VLGFSLSGGT QTPSVYDESG STGGGDDSTP SILPAPRGYP    50
    GQVCANDSDT LELPDSSRAL LLGWVPTRLV PALYGLVLVV GLPANGLALW 100
    VLATQAPRLP STMLLMNLAA ADLLLALALP PRIAYHLRGQ RWPFGEAACR 150
    LATAALYGHM YGSVLLLAAV SLDRYLALVH PLRARALRGR RLALGLCMAA 200
    WLMAAALALP LTLQRQTFRL ARSDRVLCHD ALPLDAQASH WQPAFTCLAL 250
    LGCFLPLLAM LLCYGATLHT LAASGRRYGH ALRLTAVVLA SAVAFFVPSN 300
    LLLLLHYSDP SPSAWGNLYG AYVPSLALST LNSCVDPFIY YYVSAEFRDK 350
    VRAGLFQRSP GDTVASKASA EGGSRGMGTH SSLLQ 385
    Length:385
    Mass (Da):41,133
    Last modified:October 17, 2006 - v3
    Checksum:iFACE32F10D5C561E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201A → T.3 Publications
    Corresponds to variant rs773902 [ dbSNP | Ensembl ].
    VAR_028300
    Natural varianti215 – 2151R → Q.
    Corresponds to variant rs2230799 [ dbSNP | Ensembl ].
    VAR_028301
    Natural varianti296 – 2961F → V.1 Publication
    Corresponds to variant rs2227346 [ dbSNP | Ensembl ].
    VAR_012852
    Natural varianti310 – 3101P → L.1 Publication
    Corresponds to variant rs2227376 [ dbSNP | Ensembl ].
    VAR_012853

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF080214 mRNA. Translation: AAC28860.1.
    AF055917 mRNA. Translation: AAC25699.1.
    AY431102 mRNA. Translation: AAR08487.1.
    AF384819 Genomic DNA. Translation: AAK61908.1.
    BC074782 mRNA. Translation: AAH74782.2.
    CCDSiCCDS12350.1.
    RefSeqiNP_003941.2. NM_003950.2.
    UniGeneiHs.137574.

    Genome annotation databases

    EnsembliENST00000248076; ENSP00000248076; ENSG00000127533.
    GeneIDi9002.
    KEGGihsa:9002.
    UCSCiuc002nfa.3. human.

    Polymorphism databases

    DMDMi116242700.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF080214 mRNA. Translation: AAC28860.1 .
    AF055917 mRNA. Translation: AAC25699.1 .
    AY431102 mRNA. Translation: AAR08487.1 .
    AF384819 Genomic DNA. Translation: AAK61908.1 .
    BC074782 mRNA. Translation: AAH74782.2 .
    CCDSi CCDS12350.1.
    RefSeqi NP_003941.2. NM_003950.2.
    UniGenei Hs.137574.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZPK X-ray 1.80 P/Q 46-53 [» ]
    3QDZ X-ray 2.80 E/F 39-47 [» ]
    ProteinModelPortali Q96RI0.
    SMRi Q96RI0. Positions 51-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000248076.

    Chemistry

    BindingDBi Q96RI0.
    ChEMBLi CHEMBL4691.
    GuidetoPHARMACOLOGYi 350.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q96RI0.

    Polymorphism databases

    DMDMi 116242700.

    Proteomic databases

    PaxDbi Q96RI0.
    PRIDEi Q96RI0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000248076 ; ENSP00000248076 ; ENSG00000127533 .
    GeneIDi 9002.
    KEGGi hsa:9002.
    UCSCi uc002nfa.3. human.

    Organism-specific databases

    CTDi 9002.
    GeneCardsi GC19P017365.
    HGNCi HGNC:3540. F2RL3.
    MIMi 602779. gene.
    neXtProti NX_Q96RI0.
    PharmGKBi PA27949.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG148814.
    HOGENOMi HOG000116291.
    HOVERGENi HBG096365.
    InParanoidi Q96RI0.
    KOi K04236.
    OMAi YYVSAEF.
    OrthoDBi EOG79PJPM.
    PhylomeDBi Q96RI0.
    TreeFami TF350010.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

    Miscellaneous databases

    EvolutionaryTracei Q96RI0.
    GeneWikii F2RL3.
    GenomeRNAii 9002.
    NextBioi 33761.
    PMAP-CutDB Q96RI0.
    PROi Q96RI0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q96RI0.
    CleanExi HS_F2RL3.
    Genevestigatori Q96RI0.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003944. Prot_act_rcpt_4.
    IPR003912. Protea_act_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR01428. PROTEASEAR.
    PR01430. PROTEASEAR4.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene and locus structure and chromosomal localization of the protease-activated receptor gene family."
      Kahn M.L., Hammes S.R., Botka C., Coughlin S.R.
      J. Biol. Chem. 273:23290-23296(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-47 AND ARG-68, VARIANT THR-120.
      Tissue: Lymphoma.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      King M.M., Aronstam R.S., Sharma S.V.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    5. SeattleSNPs variation discovery resource
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-296 AND LEU-310.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin."
      Kahn M.L., Nakanishi-Matsui M., Shapiro M.J., Ishihara H., Coughlin S.R.
      J. Clin. Invest. 103:879-887(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPAR4_HUMAN
    AccessioniPrimary (citable) accession number: Q96RI0
    Secondary accession number(s): O76067, Q6DK42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3