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Q96RF0 (SNX18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorting nexin-18
Alternative name(s):
SH3 and PX domain-containing protein 3B
Sorting nexin-associated Golgi protein 1
Gene names
Name:SNX18
Synonyms:SH3PXD3B, SNAG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length628 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane. Ref.6 Ref.8 Ref.9 Ref.10

Subunit structure

Heterodimer with SNX9. Interacts with ITCH. Interacts with dynamin, SYNJ1 and WASL. Interacts with the AP-1 complex. Ref.6 Ref.7 Ref.8

Subcellular location

Endomembrane system; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Partially colocalized with clathrin and dynamin at the cell membrane. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Ref.6 Ref.8 Ref.9 Ref.10

Domain

The PX domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate.

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 BAR domain.

Contains 1 PX (phox homology) domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Endocytosis
Mitosis
Protein transport
Transport
   Cellular componentCell membrane
Cytoplasmic vesicle
Endosome
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSH3 domain
   LigandLipid-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcleavage furrow formation

Inferred from mutant phenotype Ref.10. Source: UniProtKB

endocytosis

Inferred from direct assay Ref.8. Source: UniProtKB

endosomal transport

Inferred from mutant phenotype Ref.10. Source: UniProtKB

intracellular protein transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

mitotic cytokinesis

Inferred from mutant phenotype Ref.10. Source: UniProtKB

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of GTPase activity

Inferred from direct assay Ref.8. Source: UniProtKB

   Cellular_componentcytoplasmic membrane-bounded vesicle

Inferred from direct assay Ref.10. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endosome

Inferred from Biological aspect of Ancestor. Source: RefGenome

endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular vesicular exosome

Inferred from direct assay PubMed 15326289PubMed 19056867PubMed 23376485. Source: UniProt

extrinsic component of cytoplasmic side of plasma membrane

Inferred from direct assay Ref.8. Source: UniProtKB

growth cone

Inferred from electronic annotation. Source: Ensembl

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionphosphatidylinositol-4,5-bisphosphate binding

Inferred from direct assay PubMed 23878278. Source: FlyBase

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96RF0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96RF0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     542-628: KAWPLEQVIW...RVPLMTVLSF → ALTKVKESRR...EEALHKYDSV
Isoform 3 (identifier: Q96RF0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     592-628: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 628628Sorting nexin-18
PRO_0000213866

Regions

Domain1 – 6161SH3
Domain276 – 386111PX
Domain421 – 628208BAR

Sites

Binding site3121Phosphatidylinositol 4,5-bisphosphate By similarity
Binding site3141Phosphatidylinositol 4,5-bisphosphate By similarity
Binding site3381Phosphatidylinositol 4,5-bisphosphate By similarity
Binding site3521Phosphatidylinositol 4,5-bisphosphate By similarity

Natural variations

Alternative sequence542 – 62887KAWPL…TVLSF → ALTKVKESRRHVEEGKMEVQ KADGIQDRCNTISFATLAEI HHFHQIRVRDFKSQMQHFLQ QQIIFFQKVTQKLEEALHKY DSV in isoform 2.
VSP_035839
Alternative sequence592 – 62837Missing in isoform 3.
VSP_045476
Natural variant5711E → D. Ref.1 Ref.2
Corresponds to variant rs2548612 [ dbSNP | Ensembl ].
VAR_052480
Natural variant5931K → T. Ref.5
Corresponds to variant rs13162502 [ dbSNP | Ensembl ].
VAR_052481

Experimental info

Sequence conflict321E → G in BAG65075. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: BE7B16835BA80F9C

FASTA62868,894
        10         20         30         40         50         60 
MALRARALYD FRSENPGEIS LREHEVLSLC SEQDIEGWLE GVNSRGDRGL FPASYVQVIR 

        70         80         90        100        110        120 
APEPGPAGDG GPGAPARYAN VPPGGFEPLP VAPPASFKPP PDAFQALLQP QQAPPPSTFQ 

       130        140        150        160        170        180 
PPGAGFPYGG GALQPSPQQL YGGYQASQGS DDDWDDEWDD SSTVADEPGA LGSGAYPDLD 

       190        200        210        220        230        240 
GSSSAGVGAA GRYRLSTRSD LSLGSRGGSV PPQHHPSGPK SSATVSRNLN RFSTFVKSGG 

       250        260        270        280        290        300 
EAFVLGEASG FVKDGDKLCV VLGPYGPEWQ ENPYPFQCTI DDPTKQTKFK GMKSYISYKL 

       310        320        330        340        350        360 
VPTHTQVPVH RRYKHFDWLY ARLAEKFPVI SVPHLPEKQA TGRFEEDFIS KRRKGLIWWM 

       370        380        390        400        410        420 
NHMASHPVLA QCDVFQHFLT CPSSTDEKAW KQGKRKAEKD EMVGANFFLT LSTPPAAALD 

       430        440        450        460        470        480 
LQEVESKIDG FKCFTKKMDD SALQLNHTAN EFARKQVTGF KKEYQKVGQS FRGLSQAFEL 

       490        500        510        520        530        540 
DQQAFSVGLN QAIAFTGDAY DAIGELFAEQ PRQDLDPVMD LLALYQGHLA NFPDIIHVQK 

       550        560        570        580        590        600 
GKAWPLEQVI WSVLCRLKGA TLTAVPLWVS ESYSTGEEAS RDVDAWVFSL ECKLDCSTGS 

       610        620 
FLLEYLALGN EYSFSKVQRV PLMTVLSF 

« Hide

Isoform 2 [UniParc].

Checksum: A348EFB754F465E9
Show »

FASTA62469,006
Isoform 3 [UniParc].

Checksum: 43574BCAEBF15EDD
Show »

FASTA59164,752

References

« Hide 'large scale' references
[1]"A novel SH3-, Pro-rich-, and PX-domain protein of the sorting nexin family."
Hong W.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ASP-571.
Tissue: Cerebellum.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT ASP-571.
Tissue: Trachea.
[3]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-593.
Tissue: Placenta.
[6]"SNX18 is an SNX9 paralog that acts as a membrane tubulator in AP-1-positive endosomal trafficking."
Haberg K., Lundmark R., Carlsson S.R.
J. Cell Sci. 121:1495-1505(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE AP-1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"The E3 ubiquitin ligase Itch regulates sorting nexin 9 through an unconventional substrate recognition domain."
Baumann C., Lindholm C.K., Rimoldi D., Levy F.
FEBS J. 277:2803-2814(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ITCH.
[8]"SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane."
Park J., Kim Y., Lee S., Park J.J., Park Z.Y., Sun W., Kim H., Chang S.
J. Cell Sci. 123:1742-1750(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DYNAMIN; WASL AND SYNJ1, SUBUNIT.
[9]"The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins."
Wang J.T., Kerr M.C., Karunaratne S., Jeanes A., Yap A.S., Teasdale R.D.
PLoS ONE 5:E13763-E13763(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"SNX9, SNX18 and SNX33 are required for progression through and completion of mitosis."
Ma M.P., Chircop M.
J. Cell Sci. 125:4372-4382(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF395536 mRNA. Translation: AAK82415.1.
AK304195 mRNA. Translation: BAG65075.1.
AC091888 Genomic DNA. No translation available.
CH471123 Genomic DNA. Translation: EAW54894.1.
BC060791 mRNA. Translation: AAH60791.1.
BC067860 mRNA. Translation: AAH67860.1.
BC117218 mRNA. Translation: AAI17219.1.
BC117220 mRNA. Translation: AAI17221.1.
CCDSCCDS3962.1. [Q96RF0-1]
CCDS43317.1. [Q96RF0-2]
CCDS54851.1. [Q96RF0-3]
RefSeqNP_001096045.1. NM_001102575.1. [Q96RF0-2]
NP_001138899.1. NM_001145427.1. [Q96RF0-3]
NP_443102.2. NM_052870.2. [Q96RF0-1]
UniGeneHs.432755.
Hs.606166.

3D structure databases

ProteinModelPortalQ96RF0.
SMRQ96RF0. Positions 6-59, 260-541.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125193. 7 interactions.
IntActQ96RF0. 8 interactions.
STRING9606.ENSP00000317332.

PTM databases

PhosphoSiteQ96RF0.

Polymorphism databases

DMDM215273942.

Proteomic databases

MaxQBQ96RF0.
PaxDbQ96RF0.
PRIDEQ96RF0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000326277; ENSP00000317332; ENSG00000178996. [Q96RF0-1]
ENST00000343017; ENSP00000342276; ENSG00000178996. [Q96RF0-3]
ENST00000381410; ENSP00000370817; ENSG00000178996. [Q96RF0-2]
GeneID112574.
KEGGhsa:112574.
UCSCuc003jpi.4. human. [Q96RF0-2]
uc003jpj.4. human. [Q96RF0-1]

Organism-specific databases

CTD112574.
GeneCardsGC05P053850.
H-InvDBHIX0174367.
HGNCHGNC:19245. SNX18.
HPAHPA037800.
neXtProtNX_Q96RF0.
PharmGKBPA162404304.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5391.
HOGENOMHOG000261633.
HOVERGENHBG009996.
InParanoidQ96RF0.
KOK17923.
OMADPIMDLL.
OrthoDBEOG7ZKS9P.
PhylomeDBQ96RF0.
TreeFamTF314082.

Gene expression databases

BgeeQ96RF0.
CleanExHS_SNX18.
GenevestigatorQ96RF0.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
IPR001452. SH3_domain.
IPR028646. SNX18.
IPR014536. Snx9_subfam.
IPR019497. Sorting_nexin_WASP-bd-dom.
[Graphical view]
PANTHERPTHR10555:SF13. PTHR10555:SF13. 1 hit.
PfamPF10456. BAR_3_WASP_bdg. 1 hit.
PF00787. PX. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFPIRSF027744. Snx9. 1 hit.
SMARTSM00312. PX. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSNAG1.
GenomeRNAi112574.
NextBio78612.
PROQ96RF0.

Entry information

Entry nameSNX18_HUMAN
AccessionPrimary (citable) accession number: Q96RF0
Secondary accession number(s): B4E2B3 expand/collapse secondary AC list , H7BXX3, Q05BB3, Q0VG02
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM