SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q96RE9

- ZN300_HUMAN

UniProt

Q96RE9 - ZN300_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zinc finger protein 300

Gene
ZNF300
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a transcriptional repressor activity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri269 – 29123C2H2-type 1Add
BLAST
Zinc fingeri297 – 31923C2H2-type 2Add
BLAST
Zinc fingeri325 – 34723C2H2-type 3Add
BLAST
Zinc fingeri353 – 37523C2H2-type 4Add
BLAST
Zinc fingeri381 – 40323C2H2-type 5Add
BLAST
Zinc fingeri409 – 43123C2H2-type 6Add
BLAST
Zinc fingeri437 – 45923C2H2-type 7Add
BLAST
Zinc fingeri465 – 48723C2H2-type 8Add
BLAST
Zinc fingeri493 – 51523C2H2-type 9Add
BLAST
Zinc fingeri521 – 54323C2H2-type 10Add
BLAST
Zinc fingeri549 – 57123C2H2-type 11Add
BLAST
Zinc fingeri577 – 59923C2H2-type 12Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB
  4. sequence-specific DNA binding transcription factor activity Source: RefGenome

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: RefGenome
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 300
Gene namesi
Name:ZNF300
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:13091. ZNF300.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37666.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Zinc finger protein 300PRO_0000047521Add
BLAST

Proteomic databases

MaxQBiQ96RE9.
PaxDbiQ96RE9.
PRIDEiQ96RE9.

PTM databases

PhosphoSiteiQ96RE9.

Expressioni

Tissue specificityi

Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis.2 Publications

Gene expression databases

ArrayExpressiQ96RE9.
BgeeiQ96RE9.
CleanExiHS_ZNF300.
GenevestigatoriQ96RE9.

Organism-specific databases

HPAiHPA051777.

Interactioni

Subunit structurei

Interacts (via the KRAB domain) with TRIM28 (via the RBCC domain).1 Publication

Protein-protein interaction databases

BioGridi124898. 1 interaction.
IntActiQ96RE9. 2 interactions.
MINTiMINT-8376034.
STRINGi9606.ENSP00000397178.

Structurei

3D structure databases

ProteinModelPortaliQ96RE9.
SMRiQ96RE9. Positions 8-49, 185-600.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 7972KRABAdd
BLAST

Sequence similaritiesi

Contains 12 C2H2-type zinc fingers.
Contains 1 KRAB domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri269 – 29123C2H2-type 1Add
BLAST
Zinc fingeri297 – 31923C2H2-type 2Add
BLAST
Zinc fingeri325 – 34723C2H2-type 3Add
BLAST
Zinc fingeri353 – 37523C2H2-type 4Add
BLAST
Zinc fingeri381 – 40323C2H2-type 5Add
BLAST
Zinc fingeri409 – 43123C2H2-type 6Add
BLAST
Zinc fingeri437 – 45923C2H2-type 7Add
BLAST
Zinc fingeri465 – 48723C2H2-type 8Add
BLAST
Zinc fingeri493 – 51523C2H2-type 9Add
BLAST
Zinc fingeri521 – 54323C2H2-type 10Add
BLAST
Zinc fingeri549 – 57123C2H2-type 11Add
BLAST
Zinc fingeri577 – 59923C2H2-type 12Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOVERGENiHBG018163.
InParanoidiQ96RE9.
KOiK09228.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ96RE9.

Family and domain databases

Gene3Di3.30.160.60. 12 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 12 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96RE9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMKSQGLVSF KDVAVDFTQE EWQQLDPSQR TLYRDVMLEN YSHLVSMGYP    50
VSKPDVISKL EQGEEPWIIK GDISNWIYPD EYQADGRQDR KSNLHNSQSC 100
ILGTVSFHHK ILKGVTRDGS LCSILKVCQG DGQLQRFLEN QDKLFRQVTF 150
VNSKTVTEAS GHKYNPLGKI FQECIETDIS IQRFHKYDAF KKNLKPNIDL 200
PSCYKSNSRK KPDQSFGGGK SSSQSEPNSN LEKIHNGVIP FDDNQCGNVF 250
RNTQSLIQYQ NVETKEKSCV CVTCGKAFAK KSQLIVHQRI HTGKKPYDCG 300
ACGKAFSEKF HLVVHQRTHT GEKPYDCSEC GKAFSQKSSL IIHQRVHTGE 350
KPYECSECGK AFSQKSPLII HQRIHTGEKP YECRECGKAF SQKSQLIIHH 400
RAHTGEKPYE CTECGKAFCE KSHLIIHKRI HTGEKPYKCA QCEEAFSRKT 450
ELITHQLVHT GEKPYECTEC GKTFSRKSQL IIHQRTHTGE KPYKCSECGK 500
AFCQKSHLIG HQRIHTGEKP YICTECGKAF SQKSHLPGHQ RIHTGEKPYI 550
CAECGKAFSQ KSDLVLHQRI HTGERPYQCA ICGKAFIQKS QLTVHQRIHT 600
VVKS 604
Length:604
Mass (Da):68,743
Last modified:December 1, 2001 - v1
Checksum:iEABCF821E9FAC441
GO
Isoform 2 (identifier: Q96RE9-2) [UniParc]FASTAAdd to Basket

Also known as: Zinc finger protein 300-B, ZNF300B

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQK

Show »
Length:619
Mass (Da):70,300
Checksum:iB21C77AF8C8BFBB3
GO
Isoform 3 (identifier: Q96RE9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQKM

Note: No experimental confirmation available.

Show »
Length:620
Mass (Da):70,431
Checksum:i45E71F07512FCA5E
GO
Isoform 4 (identifier: Q96RE9-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Note: No experimental confirmation available.

Show »
Length:568
Mass (Da):64,484
Checksum:i093ED8AC23D7E6D4
GO

Sequence cautioni

The sequence EAW61702.1 differs from that shown. Reason: Erroneous gene model prediction.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti336 – 3361Q → H.
Corresponds to variant rs1988688 [ dbSNP | Ensembl ].
VAR_012355

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 4. VSP_047506Add
BLAST
Alternative sequencei1 – 11M → MVPAETSSSGLLEEQK in isoform 2. VSP_036830
Alternative sequencei1 – 11M → MVPAETSSSGLLEEQKM in isoform 3. VSP_036831

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531T → I in CAI46270. 1 Publication
Sequence conflicti535 – 5351H → Q in CAI46270. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF395541 mRNA. Translation: AAK83888.1.
DQ908918 mRNA. Translation: ABI81769.1.
AK096465 mRNA. Translation: BAG53297.1.
AK300528 mRNA. Translation: BAG62240.1.
DB094933 mRNA. No translation available.
AC010441 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61702.1. Sequence problems.
BC117248 mRNA. Translation: AAI17249.1.
CR933727 mRNA. Translation: CAI46270.1.
CCDSiCCDS4311.2. [Q96RE9-1]
CCDS54939.1. [Q96RE9-4]
CCDS54940.1. [Q96RE9-3]
RefSeqiNP_001166302.1. NM_001172831.1. [Q96RE9-3]
NP_001166303.1. NM_001172832.1. [Q96RE9-4]
NP_443092.1. NM_052860.2. [Q96RE9-1]
XP_006714869.1. XM_006714806.1. [Q96RE9-1]
UniGeneiHs.134885.

Genome annotation databases

EnsembliENST00000274599; ENSP00000274599; ENSG00000145908. [Q96RE9-1]
ENST00000418587; ENSP00000392593; ENSG00000145908. [Q96RE9-4]
ENST00000446148; ENSP00000397178; ENSG00000145908.
GeneIDi91975.
KEGGihsa:91975.
UCSCiuc021yfy.1. human. [Q96RE9-1]

Polymorphism databases

DMDMi20141015.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF395541 mRNA. Translation: AAK83888.1 .
DQ908918 mRNA. Translation: ABI81769.1 .
AK096465 mRNA. Translation: BAG53297.1 .
AK300528 mRNA. Translation: BAG62240.1 .
DB094933 mRNA. No translation available.
AC010441 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61702.1 . Sequence problems.
BC117248 mRNA. Translation: AAI17249.1 .
CR933727 mRNA. Translation: CAI46270.1 .
CCDSi CCDS4311.2. [Q96RE9-1 ]
CCDS54939.1. [Q96RE9-4 ]
CCDS54940.1. [Q96RE9-3 ]
RefSeqi NP_001166302.1. NM_001172831.1. [Q96RE9-3 ]
NP_001166303.1. NM_001172832.1. [Q96RE9-4 ]
NP_443092.1. NM_052860.2. [Q96RE9-1 ]
XP_006714869.1. XM_006714806.1. [Q96RE9-1 ]
UniGenei Hs.134885.

3D structure databases

ProteinModelPortali Q96RE9.
SMRi Q96RE9. Positions 8-49, 185-600.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124898. 1 interaction.
IntActi Q96RE9. 2 interactions.
MINTi MINT-8376034.
STRINGi 9606.ENSP00000397178.

PTM databases

PhosphoSitei Q96RE9.

Polymorphism databases

DMDMi 20141015.

Proteomic databases

MaxQBi Q96RE9.
PaxDbi Q96RE9.
PRIDEi Q96RE9.

Protocols and materials databases

DNASUi 91975.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000274599 ; ENSP00000274599 ; ENSG00000145908 . [Q96RE9-1 ]
ENST00000418587 ; ENSP00000392593 ; ENSG00000145908 . [Q96RE9-4 ]
ENST00000446148 ; ENSP00000397178 ; ENSG00000145908 .
GeneIDi 91975.
KEGGi hsa:91975.
UCSCi uc021yfy.1. human. [Q96RE9-1 ]

Organism-specific databases

CTDi 91975.
GeneCardsi GC05M150254.
HGNCi HGNC:13091. ZNF300.
HPAi HPA051777.
MIMi 612429. gene.
neXtProti NX_Q96RE9.
PharmGKBi PA37666.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5048.
HOVERGENi HBG018163.
InParanoidi Q96RE9.
KOi K09228.
OrthoDBi EOG7KSX7Q.
PhylomeDBi Q96RE9.

Enzyme and pathway databases

Reactomei REACT_12627. Generic Transcription Pathway.

Miscellaneous databases

GeneWikii ZNF300.
GenomeRNAii 91975.
NextBioi 77542.
PROi Q96RE9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q96RE9.
Bgeei Q96RE9.
CleanExi HS_ZNF300.
Genevestigatori Q96RE9.

Family and domain databases

Gene3Di 3.30.160.60. 12 hits.
InterProi IPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF01352. KRAB. 1 hit.
[Graphical view ]
SMARTi SM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 12 hits.
[Graphical view ]
SUPFAMi SSF109640. SSF109640. 1 hit.
PROSITEi PS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and functional analysis of a novel human KRAB/C2H2 zinc finger gene ZNF300."
    Gou D.-M., Wang J., Gao L., Sun Y., Peng X., Huang J., Li W.-X.
    Biochim. Biophys. Acta 1676:203-209(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Molecular cloning and characterization of a novel splice variant of human ZNF300 gene, which expressed highly in testis."
    Cao Y., Li J.-X., Ji C.-N., Xu X.-W., Wu M.
    DNA Seq. 18:312-315(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-38 (ISOFORM 4).
    Tissue: Brain, Prostate and Testis.
  4. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 89-604 (ISOFORMS 1/2/3/4).
    Tissue: Fetal brain.
  8. "Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1."
    Wang W., Cai J., Wu Y., Hu L., Chen Z., Hu J., Chen Z., Li W., Guo M., Huang Z.
    Cell. Mol. Life Sci. 70:3947-3958(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF268, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiZN300_HUMAN
AccessioniPrimary (citable) accession number: Q96RE9
Secondary accession number(s): A8MY91
, B3KU35, B4DU78, F5GWS1, Q06DQ3, Q17RP3, Q5H9N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi