Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein 300

Gene

ZNF300

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a transcriptional repressor activity.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 291C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri297 – 319C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri325 – 347C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri353 – 375C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri381 – 403C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri409 – 431C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri437 – 459C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri465 – 487C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri493 – 515C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri521 – 543C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri549 – 571C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri577 – 599C2H2-type 12PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000145908-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 300
Gene namesi
Name:ZNF300
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:13091. ZNF300.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi91975.
OpenTargetsiENSG00000145908.
PharmGKBiPA37666.

Polymorphism and mutation databases

BioMutaiZNF300.
DMDMi20141015.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475211 – 604Zinc finger protein 300Add BLAST604

Proteomic databases

MaxQBiQ96RE9.
PaxDbiQ96RE9.
PeptideAtlasiQ96RE9.
PRIDEiQ96RE9.

PTM databases

iPTMnetiQ96RE9.
PhosphoSitePlusiQ96RE9.

Expressioni

Tissue specificityi

Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis.2 Publications

Gene expression databases

BgeeiENSG00000145908.
CleanExiHS_ZNF300.
ExpressionAtlasiQ96RE9. baseline and differential.
GenevisibleiQ96RE9. HS.

Organism-specific databases

HPAiHPA051777.

Interactioni

Subunit structurei

Interacts (via the KRAB domain) with TRIM28 (via the RBCC domain).1 Publication

Protein-protein interaction databases

BioGridi124898. 1 interactor.
IntActiQ96RE9. 2 interactors.
MINTiMINT-8376034.
STRINGi9606.ENSP00000397178.

Structurei

3D structure databases

ProteinModelPortaliQ96RE9.
SMRiQ96RE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 79KRABPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Contains 12 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 291C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri297 – 319C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri325 – 347C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri353 – 375C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri381 – 403C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri409 – 431C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri437 – 459C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri465 – 487C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri493 – 515C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri521 – 543C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri549 – 571C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri577 – 599C2H2-type 12PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00860000133681.
HOVERGENiHBG018163.
InParanoidiQ96RE9.
KOiK09228.
OMAiCAQCEEA.
OrthoDBiEOG091G02KC.
PhylomeDBiQ96RE9.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 12 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 12 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96RE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMKSQGLVSF KDVAVDFTQE EWQQLDPSQR TLYRDVMLEN YSHLVSMGYP
60 70 80 90 100
VSKPDVISKL EQGEEPWIIK GDISNWIYPD EYQADGRQDR KSNLHNSQSC
110 120 130 140 150
ILGTVSFHHK ILKGVTRDGS LCSILKVCQG DGQLQRFLEN QDKLFRQVTF
160 170 180 190 200
VNSKTVTEAS GHKYNPLGKI FQECIETDIS IQRFHKYDAF KKNLKPNIDL
210 220 230 240 250
PSCYKSNSRK KPDQSFGGGK SSSQSEPNSN LEKIHNGVIP FDDNQCGNVF
260 270 280 290 300
RNTQSLIQYQ NVETKEKSCV CVTCGKAFAK KSQLIVHQRI HTGKKPYDCG
310 320 330 340 350
ACGKAFSEKF HLVVHQRTHT GEKPYDCSEC GKAFSQKSSL IIHQRVHTGE
360 370 380 390 400
KPYECSECGK AFSQKSPLII HQRIHTGEKP YECRECGKAF SQKSQLIIHH
410 420 430 440 450
RAHTGEKPYE CTECGKAFCE KSHLIIHKRI HTGEKPYKCA QCEEAFSRKT
460 470 480 490 500
ELITHQLVHT GEKPYECTEC GKTFSRKSQL IIHQRTHTGE KPYKCSECGK
510 520 530 540 550
AFCQKSHLIG HQRIHTGEKP YICTECGKAF SQKSHLPGHQ RIHTGEKPYI
560 570 580 590 600
CAECGKAFSQ KSDLVLHQRI HTGERPYQCA ICGKAFIQKS QLTVHQRIHT

VVKS
Length:604
Mass (Da):68,743
Last modified:December 1, 2001 - v1
Checksum:iEABCF821E9FAC441
GO
Isoform 2 (identifier: Q96RE9-2) [UniParc]FASTAAdd to basket
Also known as: Zinc finger protein 300-B, ZNF300B

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQK

Show »
Length:619
Mass (Da):70,300
Checksum:iB21C77AF8C8BFBB3
GO
Isoform 3 (identifier: Q96RE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQKM

Note: No experimental confirmation available.
Show »
Length:620
Mass (Da):70,431
Checksum:i45E71F07512FCA5E
GO
Isoform 4 (identifier: Q96RE9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Note: No experimental confirmation available.
Show »
Length:568
Mass (Da):64,484
Checksum:i093ED8AC23D7E6D4
GO

Sequence cautioni

The sequence EAW61702 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253T → I in CAI46270 (PubMed:17974005).Curated1
Sequence conflicti535H → Q in CAI46270 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_012355336Q → H.Corresponds to variant rs1988688dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0475061 – 36Missing in isoform 4. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_0368301M → MVPAETSSSGLLEEQK in isoform 2. 1 Publication1
Alternative sequenceiVSP_0368311M → MVPAETSSSGLLEEQKM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF395541 mRNA. Translation: AAK83888.1.
DQ908918 mRNA. Translation: ABI81769.1.
AK096465 mRNA. Translation: BAG53297.1.
AK300528 mRNA. Translation: BAG62240.1.
DB094933 mRNA. No translation available.
AC010441 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61702.1. Sequence problems.
BC117248 mRNA. Translation: AAI17249.1.
CR933727 mRNA. Translation: CAI46270.1.
CCDSiCCDS4311.2. [Q96RE9-1]
CCDS54939.1. [Q96RE9-4]
CCDS54940.1. [Q96RE9-3]
RefSeqiNP_001166302.1. NM_001172831.1. [Q96RE9-3]
NP_001166303.1. NM_001172832.1. [Q96RE9-4]
NP_443092.1. NM_052860.2. [Q96RE9-1]
UniGeneiHs.134885.

Genome annotation databases

EnsembliENST00000274599; ENSP00000274599; ENSG00000145908. [Q96RE9-1]
ENST00000418587; ENSP00000392593; ENSG00000145908. [Q96RE9-4]
ENST00000446148; ENSP00000397178; ENSG00000145908. [Q96RE9-3]
GeneIDi91975.
KEGGihsa:91975.
UCSCiuc021yfx.1. human. [Q96RE9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF395541 mRNA. Translation: AAK83888.1.
DQ908918 mRNA. Translation: ABI81769.1.
AK096465 mRNA. Translation: BAG53297.1.
AK300528 mRNA. Translation: BAG62240.1.
DB094933 mRNA. No translation available.
AC010441 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61702.1. Sequence problems.
BC117248 mRNA. Translation: AAI17249.1.
CR933727 mRNA. Translation: CAI46270.1.
CCDSiCCDS4311.2. [Q96RE9-1]
CCDS54939.1. [Q96RE9-4]
CCDS54940.1. [Q96RE9-3]
RefSeqiNP_001166302.1. NM_001172831.1. [Q96RE9-3]
NP_001166303.1. NM_001172832.1. [Q96RE9-4]
NP_443092.1. NM_052860.2. [Q96RE9-1]
UniGeneiHs.134885.

3D structure databases

ProteinModelPortaliQ96RE9.
SMRiQ96RE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124898. 1 interactor.
IntActiQ96RE9. 2 interactors.
MINTiMINT-8376034.
STRINGi9606.ENSP00000397178.

PTM databases

iPTMnetiQ96RE9.
PhosphoSitePlusiQ96RE9.

Polymorphism and mutation databases

BioMutaiZNF300.
DMDMi20141015.

Proteomic databases

MaxQBiQ96RE9.
PaxDbiQ96RE9.
PeptideAtlasiQ96RE9.
PRIDEiQ96RE9.

Protocols and materials databases

DNASUi91975.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274599; ENSP00000274599; ENSG00000145908. [Q96RE9-1]
ENST00000418587; ENSP00000392593; ENSG00000145908. [Q96RE9-4]
ENST00000446148; ENSP00000397178; ENSG00000145908. [Q96RE9-3]
GeneIDi91975.
KEGGihsa:91975.
UCSCiuc021yfx.1. human. [Q96RE9-1]

Organism-specific databases

CTDi91975.
DisGeNETi91975.
GeneCardsiZNF300.
HGNCiHGNC:13091. ZNF300.
HPAiHPA051777.
MIMi612429. gene.
neXtProtiNX_Q96RE9.
OpenTargetsiENSG00000145908.
PharmGKBiPA37666.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00860000133681.
HOVERGENiHBG018163.
InParanoidiQ96RE9.
KOiK09228.
OMAiCAQCEEA.
OrthoDBiEOG091G02KC.
PhylomeDBiQ96RE9.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000145908-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.

Miscellaneous databases

GeneWikiiZNF300.
GenomeRNAii91975.
PROiQ96RE9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145908.
CleanExiHS_ZNF300.
ExpressionAtlasiQ96RE9. baseline and differential.
GenevisibleiQ96RE9. HS.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 12 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 12 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN300_HUMAN
AccessioniPrimary (citable) accession number: Q96RE9
Secondary accession number(s): A8MY91
, B3KU35, B4DU78, F5GWS1, Q06DQ3, Q17RP3, Q5H9N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.