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Q96RE9 (ZN300_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 300
Gene names
Name:ZNF300
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a transcriptional repressor activity. Ref.1

Subunit structure

Interacts (via the KRAB domain) with TRIM28 (via the RBCC domain). Ref.8

Subcellular location

Nucleus Ref.1 Ref.8.

Tissue specificity

Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis. Ref.1 Ref.2

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 12 C2H2-type zinc fingers.

Contains 1 KRAB domain.

Sequence caution

The sequence EAW61702.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96RE9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96RE9-2)

Also known as: Zinc finger protein 300-B; ZNF300B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQK
Isoform 3 (identifier: Q96RE9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVPAETSSSGLLEEQKM
Note: No experimental confirmation available.
Isoform 4 (identifier: Q96RE9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Zinc finger protein 300
PRO_0000047521

Regions

Domain8 – 7972KRAB
Zinc finger269 – 29123C2H2-type 1
Zinc finger297 – 31923C2H2-type 2
Zinc finger325 – 34723C2H2-type 3
Zinc finger353 – 37523C2H2-type 4
Zinc finger381 – 40323C2H2-type 5
Zinc finger409 – 43123C2H2-type 6
Zinc finger437 – 45923C2H2-type 7
Zinc finger465 – 48723C2H2-type 8
Zinc finger493 – 51523C2H2-type 9
Zinc finger521 – 54323C2H2-type 10
Zinc finger549 – 57123C2H2-type 11
Zinc finger577 – 59923C2H2-type 12

Natural variations

Alternative sequence1 – 3636Missing in isoform 4.
VSP_047506
Alternative sequence11M → MVPAETSSSGLLEEQK in isoform 2.
VSP_036830
Alternative sequence11M → MVPAETSSSGLLEEQKM in isoform 3.
VSP_036831
Natural variant3361Q → H.
Corresponds to variant rs1988688 [ dbSNP | Ensembl ].
VAR_012355

Experimental info

Sequence conflict2531T → I in CAI46270. Ref.7
Sequence conflict5351H → Q in CAI46270. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: EABCF821E9FAC441

FASTA60468,743
        10         20         30         40         50         60 
MMKSQGLVSF KDVAVDFTQE EWQQLDPSQR TLYRDVMLEN YSHLVSMGYP VSKPDVISKL 

        70         80         90        100        110        120 
EQGEEPWIIK GDISNWIYPD EYQADGRQDR KSNLHNSQSC ILGTVSFHHK ILKGVTRDGS 

       130        140        150        160        170        180 
LCSILKVCQG DGQLQRFLEN QDKLFRQVTF VNSKTVTEAS GHKYNPLGKI FQECIETDIS 

       190        200        210        220        230        240 
IQRFHKYDAF KKNLKPNIDL PSCYKSNSRK KPDQSFGGGK SSSQSEPNSN LEKIHNGVIP 

       250        260        270        280        290        300 
FDDNQCGNVF RNTQSLIQYQ NVETKEKSCV CVTCGKAFAK KSQLIVHQRI HTGKKPYDCG 

       310        320        330        340        350        360 
ACGKAFSEKF HLVVHQRTHT GEKPYDCSEC GKAFSQKSSL IIHQRVHTGE KPYECSECGK 

       370        380        390        400        410        420 
AFSQKSPLII HQRIHTGEKP YECRECGKAF SQKSQLIIHH RAHTGEKPYE CTECGKAFCE 

       430        440        450        460        470        480 
KSHLIIHKRI HTGEKPYKCA QCEEAFSRKT ELITHQLVHT GEKPYECTEC GKTFSRKSQL 

       490        500        510        520        530        540 
IIHQRTHTGE KPYKCSECGK AFCQKSHLIG HQRIHTGEKP YICTECGKAF SQKSHLPGHQ 

       550        560        570        580        590        600 
RIHTGEKPYI CAECGKAFSQ KSDLVLHQRI HTGERPYQCA ICGKAFIQKS QLTVHQRIHT 


VVKS 

« Hide

Isoform 2 (Zinc finger protein 300-B) (ZNF300B) [UniParc].

Checksum: B21C77AF8C8BFBB3
Show »

FASTA61970,300
Isoform 3 [UniParc].

Checksum: 45E71F07512FCA5E
Show »

FASTA62070,431
Isoform 4 [UniParc].

Checksum: 093ED8AC23D7E6D4
Show »

FASTA56864,484

References

« Hide 'large scale' references
[1]"Identification and functional analysis of a novel human KRAB/C2H2 zinc finger gene ZNF300."
Gou D.-M., Wang J., Gao L., Sun Y., Peng X., Huang J., Li W.-X.
Biochim. Biophys. Acta 1676:203-209(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Molecular cloning and characterization of a novel splice variant of human ZNF300 gene, which expressed highly in testis."
Cao Y., Li J.-X., Ji C.-N., Xu X.-W., Wu M.
DNA Seq. 18:312-315(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Fetal brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-38 (ISOFORM 4).
Tissue: Brain, Prostate and Testis.
[4]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 89-604 (ISOFORMS 1/2/3/4).
Tissue: Fetal brain.
[8]"Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1."
Wang W., Cai J., Wu Y., Hu L., Chen Z., Hu J., Chen Z., Li W., Guo M., Huang Z.
Cell. Mol. Life Sci. 70:3947-3958(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZNF268, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF395541 mRNA. Translation: AAK83888.1.
DQ908918 mRNA. Translation: ABI81769.1.
AK096465 mRNA. Translation: BAG53297.1.
AK300528 mRNA. Translation: BAG62240.1.
DB094933 mRNA. No translation available.
AC010441 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61702.1. Sequence problems.
BC117248 mRNA. Translation: AAI17249.1.
CR933727 mRNA. Translation: CAI46270.1.
CCDSCCDS4311.2. [Q96RE9-1]
CCDS54939.1. [Q96RE9-4]
RefSeqNP_001166302.1. NM_001172831.1. [Q96RE9-3]
NP_001166303.1. NM_001172832.1. [Q96RE9-4]
NP_443092.1. NM_052860.2. [Q96RE9-1]
XP_006714869.1. XM_006714806.1. [Q96RE9-1]
UniGeneHs.134885.

3D structure databases

ProteinModelPortalQ96RE9.
SMRQ96RE9. Positions 8-49, 185-600.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124898. 1 interaction.
IntActQ96RE9. 2 interactions.
MINTMINT-8376034.
STRING9606.ENSP00000397178.

PTM databases

PhosphoSiteQ96RE9.

Polymorphism databases

DMDM20141015.

Proteomic databases

MaxQBQ96RE9.
PaxDbQ96RE9.
PRIDEQ96RE9.

Protocols and materials databases

DNASU91975.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274599; ENSP00000274599; ENSG00000145908. [Q96RE9-1]
ENST00000418587; ENSP00000392593; ENSG00000145908. [Q96RE9-4]
ENST00000446148; ENSP00000397178; ENSG00000145908.
GeneID91975.
KEGGhsa:91975.
UCSCuc021yfy.1. human. [Q96RE9-1]

Organism-specific databases

CTD91975.
GeneCardsGC05M150254.
HGNCHGNC:13091. ZNF300.
HPAHPA051777.
MIM612429. gene.
neXtProtNX_Q96RE9.
PharmGKBPA37666.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOVERGENHBG018163.
InParanoidQ96RE9.
KOK09228.
OrthoDBEOG7KSX7Q.
PhylomeDBQ96RE9.

Enzyme and pathway databases

ReactomeREACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ96RE9.
BgeeQ96RE9.
CleanExHS_ZNF300.
GenevestigatorQ96RE9.

Family and domain databases

Gene3D3.30.160.60. 12 hits.
InterProIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF01352. KRAB. 1 hit.
[Graphical view]
SMARTSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 12 hits.
[Graphical view]
SUPFAMSSF109640. SSF109640. 1 hit.
PROSITEPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiZNF300.
GenomeRNAi91975.
NextBio77542.
PROQ96RE9.
SOURCESearch...

Entry information

Entry nameZN300_HUMAN
AccessionPrimary (citable) accession number: Q96RE9
Secondary accession number(s): A8MY91 expand/collapse secondary AC list , B3KU35, B4DU78, F5GWS1, Q06DQ3, Q17RP3, Q5H9N5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM