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Protein

Pannexin-1

Gene

PANX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Structural component of the gap junctions and the hemichannels. May play a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis.2 Publications

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB
  • gap junction hemi-channel activity Source: UniProtKB
  • leak channel activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • calcium ion transport Source: UniProtKB
  • cation transport Source: BHF-UCL
  • cell-cell signaling Source: GO_Central
  • positive regulation of interleukin-1 alpha secretion Source: Ensembl
  • positive regulation of interleukin-1 beta secretion Source: BHF-UCL
  • protein hexamerization Source: UniProtKB
  • response to ATP Source: Ensembl
  • response to ischemia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-HSA-112303. Electric Transmission Across Gap Junctions.
R-HSA-844456. The NLRP3 inflammasome.

Protein family/group databases

TCDBi1.A.25.2.1. the gap junction-forming innexin (innexin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pannexin-1
Gene namesi
Name:PANX1
Synonyms:MRS1
ORF Names:UNQ2529/PRO6028
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:8599. PANX1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040CytoplasmicSequence analysisAdd
BLAST
Transmembranei41 – 6121HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini62 – 10645ExtracellularSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini128 – 21790CytoplasmicSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini239 – 26628ExtracellularSequence analysisAdd
BLAST
Transmembranei267 – 28721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini288 – 426139CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • bleb Source: BHF-UCL
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • gap junction Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: BHF-UCL
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Gap junction, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi255 – 2551N → Q: Impairs glycosylation. 1 Publication
Mutagenesisi347 – 3471C → S: Leaky hemichannel conductive at resting potential. 1 Publication

Organism-specific databases

PharmGKBiPA32929.

Chemistry

DrugBankiDB01032. Probenecid.

Polymorphism and mutation databases

BioMutaiPANX1.
DMDMi317373551.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Pannexin-1PRO_0000208484Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401S-nitrosocysteineBy similarity
Glycosylationi255 – 2551N-linked (GlcNAc...)1 Publication
Modified residuei347 – 3471S-nitrosocysteineBy similarity

Post-translational modificationi

S-nitrosylation inhibits channel currents and ATP release.By similarity

Keywords - PTMi

Glycoprotein, S-nitrosylation

Proteomic databases

EPDiQ96RD7.
MaxQBiQ96RD7.
PaxDbiQ96RD7.
PRIDEiQ96RD7.

PTM databases

iPTMnetiQ96RD7.
PhosphoSiteiQ96RD7.

Expressioni

Gene expression databases

BgeeiQ96RD7.
CleanExiHS_PANX1.
ExpressionAtlasiQ96RD7. baseline and differential.
GenevisibleiQ96RD7. HS.

Organism-specific databases

HPAiHPA016930.

Interactioni

Subunit structurei

Homohexamer. Forms homomeric or PANX1/PANX2-heteromeric intercellular channels on coexpression in paired Xenopus oocytes (By similarity).By similarity

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117295. 11 interactions.
DIPiDIP-43936N.
IntActiQ96RD7. 7 interactions.
MINTiMINT-3379479.
STRINGi9606.ENSP00000227638.

Structurei

3D structure databases

ProteinModelPortaliQ96RD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pannexin family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II5X. Eukaryota.
ENOG410YA5H. LUCA.
GeneTreeiENSGT00390000009703.
HOVERGENiHBG053497.
InParanoidiQ96RD7.
KOiK03443.
OMAiVPFRQKT.
OrthoDBiEOG74N5G9.
PhylomeDBiQ96RD7.
TreeFamiTF333142.

Family and domain databases

InterProiIPR000990. Innexin.
[Graphical view]
PfamiPF00876. Innexin. 1 hit.
[Graphical view]
PROSITEiPS51013. PANNEXIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96RD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIAQLATEY VFSDFLLKEP TEPKFKGLRL ELAVDKMVTC IAVGLPLLLI
60 70 80 90 100
SLAFAQEISI GTQISCFSPS SFSWRQAAFV DSYCWAAVQQ KNSLQSESGN
110 120 130 140 150
LPLWLHKFFP YILLLFAILL YLPPLFWRFA AAPHICSDLK FIMEELDKVY
160 170 180 190 200
NRAIKAAKSA RDLDMRDGAC SVPGVTENLG QSLWEVSESH FKYPIVEQYL
210 220 230 240 250
KTKKNSNNLI IKYISCRLLT LIIILLACIY LGYYFSLSSL SDEFVCSIKS
260 270 280 290 300
GILRNDSTVP DQFQCKLIAV GIFQLLSVIN LVVYVLLAPV VVYTLFVPFR
310 320 330 340 350
QKTDVLKVYE ILPTFDVLHF KSEGYNDLSL YNLFLEENIS EVKSYKCLKV
360 370 380 390 400
LENIKSSGQG IDPMLLLTNL GMIKMDVVDG KTPMSAEMRE EQGNQTAELQ
410 420
GMNIDSETKA NNGEKNARQR LLDSSC
Length:426
Mass (Da):48,050
Last modified:January 11, 2011 - v4
Checksum:i368B48E0709424FB
GO
Isoform 2 (identifier: Q96RD7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     401-404: Missing.

Show »
Length:422
Mass (Da):47,635
Checksum:i91800555C528DED0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1854Missing in AAK73361 (Ref. 7) Curated
Sequence conflicti316 – 3161D → V in AAK73361 (Ref. 7) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51Q → H.4 Publications
Corresponds to variant rs1138800 [ dbSNP | Ensembl ].
VAR_016098
Natural varianti272 – 2721I → V.
Corresponds to variant rs12793348 [ dbSNP | Ensembl ].
VAR_031225

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei401 – 4044Missing in isoform 2. 2 PublicationsVSP_011476

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093239 mRNA. Translation: AAC61779.1.
AF398509 mRNA. Translation: AAK91714.1.
AF398508, AF398506, AF398507 Genomic DNA. Translation: AAK91713.1.
AY048509 Genomic DNA. Translation: AAL06604.1.
AY359023 mRNA. Translation: AAQ89382.1.
AK074897 mRNA. Translation: BAC11276.1.
AP003966 Genomic DNA. No translation available.
BC016931 mRNA. Translation: AAH16931.1.
AH010945 Genomic DNA. Translation: AAK73361.1.
CCDSiCCDS8296.1. [Q96RD7-1]
RefSeqiNP_056183.2. NM_015368.3. [Q96RD7-1]
UniGeneiHs.591976.

Genome annotation databases

EnsembliENST00000227638; ENSP00000227638; ENSG00000110218. [Q96RD7-1]
ENST00000436171; ENSP00000411461; ENSG00000110218. [Q96RD7-2]
GeneIDi24145.
KEGGihsa:24145.
UCSCiuc001peq.4. human. [Q96RD7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Pannexin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093239 mRNA. Translation: AAC61779.1.
AF398509 mRNA. Translation: AAK91714.1.
AF398508, AF398506, AF398507 Genomic DNA. Translation: AAK91713.1.
AY048509 Genomic DNA. Translation: AAL06604.1.
AY359023 mRNA. Translation: AAQ89382.1.
AK074897 mRNA. Translation: BAC11276.1.
AP003966 Genomic DNA. No translation available.
BC016931 mRNA. Translation: AAH16931.1.
AH010945 Genomic DNA. Translation: AAK73361.1.
CCDSiCCDS8296.1. [Q96RD7-1]
RefSeqiNP_056183.2. NM_015368.3. [Q96RD7-1]
UniGeneiHs.591976.

3D structure databases

ProteinModelPortaliQ96RD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117295. 11 interactions.
DIPiDIP-43936N.
IntActiQ96RD7. 7 interactions.
MINTiMINT-3379479.
STRINGi9606.ENSP00000227638.

Chemistry

DrugBankiDB01032. Probenecid.

Protein family/group databases

TCDBi1.A.25.2.1. the gap junction-forming innexin (innexin) family.

PTM databases

iPTMnetiQ96RD7.
PhosphoSiteiQ96RD7.

Polymorphism and mutation databases

BioMutaiPANX1.
DMDMi317373551.

Proteomic databases

EPDiQ96RD7.
MaxQBiQ96RD7.
PaxDbiQ96RD7.
PRIDEiQ96RD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000227638; ENSP00000227638; ENSG00000110218. [Q96RD7-1]
ENST00000436171; ENSP00000411461; ENSG00000110218. [Q96RD7-2]
GeneIDi24145.
KEGGihsa:24145.
UCSCiuc001peq.4. human. [Q96RD7-1]

Organism-specific databases

CTDi24145.
GeneCardsiPANX1.
H-InvDBHIX0010028.
HGNCiHGNC:8599. PANX1.
HPAiHPA016930.
MIMi608420. gene.
neXtProtiNX_Q96RD7.
PharmGKBiPA32929.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410II5X. Eukaryota.
ENOG410YA5H. LUCA.
GeneTreeiENSGT00390000009703.
HOVERGENiHBG053497.
InParanoidiQ96RD7.
KOiK03443.
OMAiVPFRQKT.
OrthoDBiEOG74N5G9.
PhylomeDBiQ96RD7.
TreeFamiTF333142.

Enzyme and pathway databases

ReactomeiR-HSA-112303. Electric Transmission Across Gap Junctions.
R-HSA-844456. The NLRP3 inflammasome.

Miscellaneous databases

ChiTaRSiPANX1. human.
GeneWikiiPANX1.
GenomeRNAii24145.
PROiQ96RD7.
SOURCEiSearch...

Gene expression databases

BgeeiQ96RD7.
CleanExiHS_PANX1.
ExpressionAtlasiQ96RD7. baseline and differential.
GenevisibleiQ96RD7. HS.

Family and domain databases

InterProiIPR000990. Innexin.
[Graphical view]
PfamiPF00876. Innexin. 1 hit.
[Graphical view]
PROSITEiPS51013. PANNEXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel cDNA of unknown function."
    Bolger G.B., Steele M.R.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT HIS-5.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), VARIANT HIS-5.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT HIS-5.
  4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT HIS-5.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Bone.
  7. "Genomic organization of putative human gap junction proteins PANX1 and PANX2."
    Baranova A.V., Ivanov D.V., Borodina T.A., Panchin Y.V., Shagin D.A., Lukyanov S.A.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-426.
  8. "Functional implications of calcium permeability of the channel formed by pannexin 1."
    Vanden Abeele F., Bidaux G., Gordienko D., Beck B., Panchin Y.V., Baranova A.V., Ivanov D.V., Skryma R., Prevarskaya N.
    J. Cell Biol. 174:535-546(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Pannexin1 channels contain a glycosylation site that targets the hexamer to the plasma membrane."
    Boassa D., Ambrosi C., Qiu F., Dahl G., Gaietta G., Sosinsky G.
    J. Biol. Chem. 282:31733-31743(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-255, HOMOHEXAMERIZATION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-255.
  10. "Intracellular cysteine 346 is essentially involved in regulating Panx1 channel activity."
    Bunse S., Schmidt M., Prochnow N., Zoidl G., Dermietzel R.
    J. Biol. Chem. 285:38444-38452(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-347.

Entry informationi

Entry nameiPANX1_HUMAN
AccessioniPrimary (citable) accession number: Q96RD7
Secondary accession number(s): O75968
, Q543A0, Q6UW26, Q96AM9, Q96L77, Q96RS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 11, 2011
Last modified: June 8, 2016
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.