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Reviewed, UniProtKB/Swiss-Prot Q96QZ7 (MAGI1_HUMAN)

Last modified July 22, 2008. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Alternative name(s):
    BAI1-associated protein 1
      Short name(s)=BAP-1
    Membrane-associated guanylate kinase inverted 1
      Short name(s)=MAGI-1
    Atrophin-1-interacting protein 3
      Short name(s)=AIP3
    WW domain-containing protein 3
      Short name(s)=WWP3
    Trinucleotide repeat-containing gene 19 protein
Gene names
Name: MAGI1
Synonyms: BAIAP1, BAP1, TNRC19
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1491 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ACCN3 currents by modulating its expression at the cell surface By similarity.

Subunit structure

Interacts through its WW 2 domain with SYNPO and through its PDZ 5 domain with ACTN4. Interacts with cytoplasmic domain of BAI1. Interacts via its WW domains with DRPLA. Interacts with ESAM, LRP2 and CXADR. May interact with CTNNB1. Interacts through its PDZ 1 domain with NET1 By similarity. Interacts with ACCN3 and AMOT. Interacts with FCHSD2. Interacts with IGSF5/JAM4 and through its PDZ 2 and 3 domains with NPHS1 forming a tripartite complex By similarity.

Subcellular location

Cell junctiontight junction. Cell membrane; Peripheral membrane protein. Note= Localizes to epithelial cells tight junctions.

Tissue specificity

Widely expressed with the exception of skeletal muscle. Isoform 1, isoform 2 and isoform 6 are highly expressed in colon, kidney, lung, liver, and pancreas. Isoform 5 is predominantly expressed in brain and heart. Isoform 3 and isoform 4 are highly expressed in pancreas and brain.

Post-translational modification

Phosphorylated.

Sequence similarities

Contains 1 guanylate kinase-like domain.

Contains 6 PDZ (DHR) domains.

Contains 2 WW domains.

Ontologies

Binary interactions

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Notes: Additional isoforms seem to exist.
Isoform 1 (identifier: Q96QZ7-1)

Also known as: MAGI-1C-alpha-beta1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Notes: No experimental confirmation available.
Isoform 2 (identifier: Q96QZ7-2)

Also known as: MAGI-1C-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     806-834: PMSPSPASGLSKGEREREINSTNFGECPI → L
     1027-1027: Missing.
Isoform 3 (identifier: Q96QZ7-3)

Also known as: MAGI-1A-alpha-beta1;

The sequence of this isoform differs from the canonical sequence as follows:
     1241-1256: DPSSDRHGPATGPQGV → GGSNYENIPSFPGMTP
     1257-1288: Missing.
     1289-1491: Missing.
Isoform 4 (identifier: Q96QZ7-4)

Also known as: MAGI-1A-alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     806-834: PMSPSPASGLSKGEREREINSTNFGECPI → L
     1027-1027: Missing.
     1028-1038: Missing.
     1039-1094: Missing.
     1241-1256: DPSSDRHGPATGPQGV → GGSNYENIPSFPGMTP
     1257-1288: Missing.
     1289-1491: Missing.
Isoform 5 (identifier: Q96QZ7-5)

Also known as: MAGI-1B-alpha-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1027-1027: Missing.
     1241-1288: DPSSDRHGPA...LHKSSPHGEK → AMIPPNIAAC...PPPVHKVFRK
     1289-1491: Missing.
Isoform 6 (identifier: Q96QZ7-6)

Also known as: MAGI-1C-beta2;

The sequence of this isoform differs from the canonical sequence as follows:
     806-834: PMSPSPASGLSKGEREREINSTNFGECPI → L
     1027-1027: Missing.
     1028-1038: Missing.
Notes: No experimental confirmation available.
Isoform 7 (identifier: Q96QZ7-7)

Also known as: MAGI-1C-beta3;

The sequence of this isoform differs from the canonical sequence as follows:
     806-834: PMSPSPASGLSKGEREREINSTNFGECPI → L
     1023-1099: GTTFAGNACV...IIPGDESSNA → VMQCQPPSWC...EVQIYSCNNP
Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 14911491Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1

Regions

Domain17 – 10589PDZ 1
Domain96 – 287192Guanylate kinase-like
Domain300 – 33334WW 1
Domain359 – 39234WW 2
Domain472 – 55483PDZ 2
Domain643 – 72179PDZ 3
Domain813 – 89583PDZ 4
Domain970 – 106697PDZ 5
Domain1124 – 120683PDZ 6
Nucleotide binding103 – 1108ATP By similarity
Region970 – 106697Interaction with FCHSD2
Compositional bias402 – 42120Poly-Gln
Compositional bias662 – 6654Poly-Gly
Compositional bias978 – 98811Poly-Gly
Compositional bias1341 – 13444Poly-Ala
Compositional bias1398 – 14014Poly-Arg

Amino acid modifications

Modified residue8661Phosphotyrosine By similarity

Natural variations

Alternative sequence806 – 83429PMSPS…GECPI → L in isoform 2, isoform 4, isoform 6 and isoform 7.
Alternative sequence1023 – 109977GTTFA…ESSNA → VMQCQPPSWCHSALGGSKHC NSVMGAASLEVQIYSCNNP in isoform 7.
Alternative sequence10271Missing in isoform 2, isoform 4, isoform 5 and isoform 6.
Alternative sequence1028 – 103811Missing in isoform 4 and isoform 6.
Alternative sequence1039 – 109456Missing in isoform 4.
Alternative sequence1241 – 128848DPSSD…PHGEK → AMIPPNIAACMRNEKLGEAC FYLMGHNQTTTPAATATAPP PVHKVFRK in isoform 5.
Alternative sequence1241 – 125616DPSSD…GPQGV → GGSNYENIPSFPGMTP in isoform 3 and isoform 4.
Alternative sequence1257 – 128832Missing in isoform 3 and isoform 4.
Alternative sequence1289 – 1491203Missing in isoform 3, isoform 4 and isoform 5.

Experimental info

Sequence conflict7751A → G in AAC04844. Ref.3

Secondary structure

............................................................... 1491
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (MAGI-1C-alpha-beta1) [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: 1898962F733CEAC1

FASTA1,491164,641
        10         20         30         40         50         60 
MSKVIQKKNH WTSRVHECTV KRGPQGELGV TVLGGAEHGE FPYVGAVAAV EAAGLPGGGE 

        70         80         90        100        110        120 
GPRLGEGELL LEVQGVRVSG LPRYDVLGVI DSCKEAVTFK AVRQGGRLNK DLRHFLNQRF 

       130        140        150        160        170        180 
QKGFPDHELQ QTIRDNLYRH AVPCTTRSPR EGEVPGVDYN FLTVKEFLDL EQSGTLLEVG 

       190        200        210        220        230        240 
TYEGNYYGTP KPPSQPVSGK VITTDALHSL QSGSKQSTPK RTKSYNDMQN AGIVHAENEE 

       250        260        270        280        290        300 
EDDVPEMNSS FTADSGEQEE HTLQETALPP VNSSIIAAPI TDPSQKFPQY LPLSAEDNLG 

       310        320        330        340        350        360 
PLPENWEMAY TENGEVYFID HNTKTTSWLD PRCLNKQQKP LEECEDDEGV HTEELDSELE 

       370        380        390        400        410        420 
LPAGWEKIED PVYGIYYVDH INRKTQYENP VLEAKRKKQL EQQQQQQQQQ QQQQQQQQQQ 

       430        440        450        460        470        480 
QTEEWTEDHS ALVPPVIPNH PPSNPEPARE VPLQGKPFFT RNPSELKGKF IHTKLRKSSR 

       490        500        510        520        530        540 
GFGFTVVGGD EPDEFLQIKS LVLDGPAALD GKMETGDVIV SVNDTCVLGH THAQVVKIFQ 

       550        560        570        580        590        600 
SIPIGASVDL ELCRGYPLPF DPDDPNTSLV TSVAILDKEP IIVNGQETYD SPASHSSKTG 

       610        620        630        640        650        660 
KVNGMKDARP SSPADVASNS SHGYPNDTVS LASSIATQPE LITVHIVKGP MGFGFTIADS 

       670        680        690        700        710        720 
PGGGGQRVKQ IVDSPRCRGL KEGDLIVEVN KKNVQALTHN QVVDMLVECP KGSEVTLLVQ 

       730        740        750        760        770        780 
RGGLPVPKKS PKSQPLERKD SQNSSQHSVS SHRSLHTASP SHSTQVLPEF PPAEAQAPDQ 

       790        800        810        820        830        840 
TDSSGQKKPD PFKIWAQSRS MYENRPMSPS PASGLSKGER EREINSTNFG ECPIPDYQEQ 

       850        860        870        880        890        900 
DIFLWRKETG FGFRILGGNE PGEPIYIGHI VPLGAADTDG RLRSGDELIC VDGTPVIGKS 

       910        920        930        940        950        960 
HQLVVQLMQQ AAKQGHVNLT VRRKVVFAVP KTENEVPSPA SSHHSSNQPA SLTEEKRTPQ 

       970        980        990       1000       1010       1020 
GSQNSLNTVS SGSGSTSGIG SGGGGGSGVV STVVQPYDVE IRRGENEGFG FVIVSSVSRP 

      1030       1040       1050       1060       1070       1080 
EAGTTFAGNA CVAMPHKIGR IIEGSPADRC GKLKVGDRIL AVNGCSITNK SHSDIVNLIK 

      1090       1100       1110       1120       1130       1140 
EAGNTVTLRI IPGDESSNAT LLTNAEKIAT ITTTHTPSQQ GTQETRNTTK PKQESQFEFK 

      1150       1160       1170       1180       1190       1200 
APQATQEQDF YTVELERGAK GFGFSLRGGR EYNMDLYVLR LAEDGPAERC GKMRIGDEIL 

      1210       1220       1230       1240       1250       1260 
EINGETTKNM KHSRAIELIK NGGRRVRLFL KRGDGSVPEY DPSSDRHGPA TGPQGVPEVR 

      1270       1280       1290       1300       1310       1320 
AGPDRRQHPS LESSYPPDLH KSSPHGEKRA HARDPKGSRE YSRQPNEHHT WNGTSRKPDS 

      1330       1340       1350       1360       1370       1380 
GACRPKDRAP EGRRDAQAER AAAANGPKRR SPEKRREGTR SADNTLERRE KHEKRRDVSP 

      1390       1400       1410       1420       1430       1440 
ERRRERSPTR RRDGSPSRRR RSLERLLEQR RSPERRRGGS PERRAKSTDR RRARSPERRR 

      1450       1460       1470       1480       1490 
ERSLDKRNRE DRASHREREE ANLKQDAGRS SRHPPEQRRR PYKECSTDLS I 

« Hide

Isoform 2 (MAGI-1C-beta) [UniParc].

Checksum: 2E98A448A9E7DF89
Show »

1,462161,610
Isoform 3 (MAGI-1A-alpha-beta1) [UniParc].

Checksum: 83FADE2091A4C8E4
Show »

1,256136,981