ID   TSN32_HUMAN             Reviewed;         320 AA.
AC   Q96QS1; Q96KX4; Q9HC50; Q9HC51; Q9Y5U1;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   24-JAN-2024, entry version 153.
DE   RecName: Full=Tetraspanin-32;
DE            Short=Tspan-32;
DE   AltName: Full=Protein Phemx;
GN   Name=TSPAN32; Synonyms=PHEMX, TSSC6;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RX   PubMed=10072438; DOI=10.1093/hmg/8.4.683;
RA   Lee M.P., Brandenburg S., Landes G.M., Adams M., Miller G., Feinberg A.P.;
RT   "Two novel genes in the center of the 11p15 imprinted domain escape genomic
RT   imprinting.";
RL   Hum. Mol. Genet. 8:683-690(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=11718897; DOI=10.1016/s0167-4781(01)00306-2;
RA   Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B.,
RA   Wright M.D.;
RT   "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6
RT   is a genuine member of the tetraspanin superfamily and is expressed
RT   specifically in haematopoietic organs.";
RL   Biochim. Biophys. Acta 1522:31-41(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 33-320 (ISOFORMS 3 AND 4), AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=10950922; DOI=10.1006/geno.2000.6277;
RA   Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J.,
RA   Hughes M.R., Ko M.S.H.;
RT   "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the
RT   imprinted cluster on distal chromosome 7.";
RL   Genomics 68:13-21(2000).
RN   [5]
RP   SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
RX   PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8;
RA   Hillman R.T., Green R.E., Brenner S.E.;
RT   "An unappreciated role for RNA surveillance.";
RL   Genome Biol. 5:R8.1-R8.16(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC         Comment=Additional isoforms seem to exist. Experimental confirmation
CC         may be lacking for some isoforms.;
CC       Name=1;
CC         IsoId=Q96QS1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96QS1-2; Sequence=VSP_003932;
CC       Name=3;
CC         IsoId=Q96QS1-3; Sequence=VSP_003937, VSP_003938;
CC       Name=4;
CC         IsoId=Q96QS1-4; Sequence=VSP_003932, VSP_003933, VSP_003934;
CC       Name=5;
CC         IsoId=Q96QS1-5; Sequence=VSP_003935, VSP_003936;
CC   -!- TISSUE SPECIFICITY: Expressed ubiquitously at low levels. High levels
CC       of expression are confined to hematopoietic tissues including
CC       peripheral blood leukocytes, thymus and spleen.
CC       {ECO:0000269|PubMed:10072438}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from early embryogenesis through to
CC       adulthood. {ECO:0000269|PubMed:10950922}.
CC   -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; AF125569; AAD23580.1; -; mRNA.
DR   EMBL; AY039001; AAK84431.1; -; mRNA.
DR   EMBL; BC016693; AAH16693.1; -; mRNA.
DR   EMBL; AF176070; AAG15840.1; -; mRNA.
DR   EMBL; AF176071; AAG15841.1; -; mRNA.
DR   CCDS; CCDS7733.1; -. [Q96QS1-1]
DR   RefSeq; NP_620591.3; NM_139022.2. [Q96QS1-1]
DR   AlphaFoldDB; Q96QS1; -.
DR   SMR; Q96QS1; -.
DR   BioGRID; 115387; 1.
DR   STRING; 9606.ENSP00000182290; -.
DR   iPTMnet; Q96QS1; -.
DR   PhosphoSitePlus; Q96QS1; -.
DR   BioMuta; TSPAN32; -.
DR   DMDM; 20139069; -.
DR   MassIVE; Q96QS1; -.
DR   PaxDb; 9606-ENSP00000182290; -.
DR   PeptideAtlas; Q96QS1; -.
DR   ProteomicsDB; 77889; -. [Q96QS1-1]
DR   ProteomicsDB; 77890; -. [Q96QS1-2]
DR   ProteomicsDB; 77891; -. [Q96QS1-3]
DR   ProteomicsDB; 77893; -. [Q96QS1-5]
DR   Antibodypedia; 10387; 205 antibodies from 28 providers.
DR   DNASU; 10077; -.
DR   Ensembl; ENST00000182290.9; ENSP00000182290.5; ENSG00000064201.16. [Q96QS1-1]
DR   Ensembl; ENST00000381121.7; ENSP00000370513.3; ENSG00000064201.16. [Q96QS1-3]
DR   Ensembl; ENST00000446063.6; ENSP00000395018.2; ENSG00000064201.16. [Q96QS1-5]
DR   GeneID; 10077; -.
DR   KEGG; hsa:10077; -.
DR   MANE-Select; ENST00000182290.9; ENSP00000182290.5; NM_139022.3; NP_620591.3.
DR   UCSC; uc001lvy.2; human. [Q96QS1-1]
DR   AGR; HGNC:13410; -.
DR   CTD; 10077; -.
DR   DisGeNET; 10077; -.
DR   GeneCards; TSPAN32; -.
DR   HGNC; HGNC:13410; TSPAN32.
DR   HPA; ENSG00000064201; Group enriched (bone marrow, heart muscle, lymphoid tissue).
DR   MIM; 603853; gene.
DR   neXtProt; NX_Q96QS1; -.
DR   OpenTargets; ENSG00000064201; -.
DR   PharmGKB; PA33257; -.
DR   VEuPathDB; HostDB:ENSG00000064201; -.
DR   eggNOG; ENOG502S0ED; Eukaryota.
DR   GeneTree; ENSGT00390000003287; -.
DR   HOGENOM; CLU_076116_0_0_1; -.
DR   InParanoid; Q96QS1; -.
DR   OMA; GQDCLRE; -.
DR   OrthoDB; 4034194at2759; -.
DR   PhylomeDB; Q96QS1; -.
DR   TreeFam; TF336277; -.
DR   PathwayCommons; Q96QS1; -.
DR   BioGRID-ORCS; 10077; 10 hits in 1144 CRISPR screens.
DR   ChiTaRS; TSPAN32; human.
DR   GeneWiki; TSPAN32; -.
DR   GenomeRNAi; 10077; -.
DR   Pharos; Q96QS1; Tbio.
DR   PRO; PR:Q96QS1; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q96QS1; Protein.
DR   Bgee; ENSG00000064201; Expressed in granulocyte and 98 other cell types or tissues.
DR   ExpressionAtlas; Q96QS1; baseline and differential.
DR   GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR   GO; GO:0070442; C:integrin alphaIIb-beta3 complex; IEA:Ensembl.
DR   GO; GO:0007267; P:cell-cell signaling; TAS:UniProtKB.
DR   GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR   GO; GO:0042832; P:defense response to protozoan; IEA:Ensembl.
DR   GO; GO:0007229; P:integrin-mediated signaling pathway; IEA:Ensembl.
DR   GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; IEA:Ensembl.
DR   GO; GO:0042130; P:negative regulation of T cell proliferation; IEA:Ensembl.
DR   GO; GO:0070527; P:platelet aggregation; IEA:Ensembl.
DR   GO; GO:0050688; P:regulation of defense response to virus; IEA:Ensembl.
DR   GO; GO:0042098; P:T cell proliferation; IEA:Ensembl.
DR   CDD; cd03153; PHEMX_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR042782; PHEMX_LEL.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   Genevisible; Q96QS1; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..320
FT                   /note="Tetraspanin-32"
FT                   /id="PRO_0000219278"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..30
FT                   /note="Missing (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:10072438,
FT                   ECO:0000303|PubMed:10950922"
FT                   /id="VSP_003932"
FT   VAR_SEQ         137..154
FT                   /note="GTSHVRRQELAAIQDVFL -> VSVLWEEVSFQPSGEHRG (in isoform
FT                   4)"
FT                   /evidence="ECO:0000303|PubMed:10950922"
FT                   /id="VSP_003933"
FT   VAR_SEQ         155..320
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:10950922"
FT                   /id="VSP_003934"
FT   VAR_SEQ         210..258
FT                   /note="VSALLFSSFLWFAIRCGCSLDRKGKYTLTPRACGRQPQEPSLLRCSQGG ->
FT                   LGPQGQIHPDPTSMWPPAPGAQPLEMLPGWTHTLSPLRSSCYWSKRMLG (in
FT                   isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_003935"
FT   VAR_SEQ         241..253
FT                   /note="ACGRQPQEPSLLR -> SPGQKSRWAQWVP (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10950922"
FT                   /id="VSP_003937"
FT   VAR_SEQ         254..320
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10950922"
FT                   /id="VSP_003938"
FT   VAR_SEQ         259..320
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_003936"
SQ   SEQUENCE   320 AA;  34631 MW;  534E4C29767B8E6D CRC64;
     MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW
     AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE
     DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR
     EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR
     ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR
     ALQGRSRGGL SGCPERGLSD
//