Q96QS1 (TSN32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraspanin-32
Short name=Tspan-32

Alternative name(s):
Protein Phemx

Gene names

Name:	TSPAN32
Synonyms:	PHEMX, TSSC6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed ubiquitously at low levels. High levels of expression are confined to hematopoietic tissues including peripheral blood leukocytes, thymus and spleen. Ref.1
Developmental stage	Expressed from early embryogenesis through to adulthood. Ref.4
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.4. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement. Source: UniProtKB intracellular Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q96QS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96QS1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Isoform 3 (identifier: Q96QS1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     241-253: ACGRQPQEPSLLR → SPGQKSRWAQWVP
     254-320: Missing.

Isoform 4 (identifier: Q96QS1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     137-154: GTSHVRRQELAAIQDVFL → VSVLWEEVSFQPSGEHRG
     155-320: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 5 (identifier: Q96QS1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     210-258: VSALLFSSFL...PSLLRCSQGG → LGPQGQIHPD...SCYWSKRMLG
     259-320: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Tetraspanin-32		PRO_0000219278
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	203 – 223	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 30	30	Missing in isoform 2 and isoform 4.		VSP_003932
	Alternative sequence	137 – 154	18	GTSHV…QDVFL → VSVLWEEVSFQPSGEHRG in isoform 4.		VSP_003933
	Alternative sequence	155 – 320	166	Missing in isoform 4.		VSP_003934
	Alternative sequence	210 – 258	49	VSALL…CSQGG → LGPQGQIHPDPTSMWPPAPG AQPLEMLPGWTHTLSPLRSS CYWSKRMLG in isoform 5.		VSP_003935
	Alternative sequence	241 – 253	13	ACGRQ…PSLLR → SPGQKSRWAQWVP in isoform 3.		VSP_003937
	Alternative sequence	254 – 320	67	Missing in isoform 3.		VSP_003938
	Alternative sequence	259 – 320	62	Missing in isoform 5.		VSP_003936

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 534E4C29767B8E6D	FASTA	320	34,631
	10 20 30 40 50 60 MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW 70 80 90 100 110 120 AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE 130 140 150 160 170 180 DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR 190 200 210 220 230 240 EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR 250 260 270 280 290 300 ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR 310 320 ALQGRSRGGL SGCPERGLSD
	Isoform 2 [UniParc]. Checksum: B9E86C72A6C9B727	FASTA	290	31,308
	10 20 30 40 50 60 MVTLTYFGAH FAVIRRASLE KNPYQAVHQW AFSAGLSLVG LLTLGAVLSA AATVREAQGL 70 80 90 100 110 120 MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ 130 140 150 160 170 180 DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR EDCLQGIRSF LRTHQQVASS LTSIGLALTV 190 200 210 220 230 240 SALLFSSFLW FAIRCGCSLD RKGKYTLTPR ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI 250 260 270 280 290 GPRGCSGSLR WLQESDAAPL PLSCHLAAHR ALQGRSRGGL SGCPERGLSD
	Isoform 3 [UniParc]. Checksum: A616383C45BE67E6	FASTA	253	27,844
	10 20 30 40 50 60 MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW 70 80 90 100 110 120 AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE 130 140 150 160 170 180 DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR 190 200 210 220 230 240 EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR 250 SPGQKSRWAQ WVP
	Isoform 4 [UniParc]. Checksum: 29B8FF48896D8BB8	FASTA	124	13,643
	10 20 30 40 50 60 MVTLTYFGAH FAVIRRASLE KNPYQAVHQW AFSAGLSLVG LLTLGAVLSA AATVREAQGL 70 80 90 100 110 120 MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE DAMLDTYDLV YEQAMKVSVL WEEVSFQPSG EHRG
	Isoform 5 [UniParc]. Checksum: 4564FAB7C7C2E9F4	FASTA	258	28,211
	10 20 30 40 50 60 MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW 70 80 90 100 110 120 AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE 130 140 150 160 170 180 DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR 190 200 210 220 230 240 EDCLQGIRSF LRTHQQVASS LTSIGLALTL GPQGQIHPDP TSMWPPAPGA QPLEMLPGWT 250 HTLSPLRSSC YWSKRMLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting." Lee M.P., Brandenburg S., Landes G.M., Adams M., Miller G., Feinberg A.P. Hum. Mol. Genet. 8:683-690(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[2]	"Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs." Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B., Wright M.D. Biochim. Biophys. Acta 1522:31-41(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Lung.
[4]	"Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7." Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J., Hughes M.R., Ko M.S.H. Genomics 68:13-21(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-320 (ISOFORMS 3 AND 4), DEVELOPMENTAL STAGE.
[5]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF125569 mRNA. Translation: AAD23580.1. AY039001 mRNA. Translation: AAK84431.1. BC016693 mRNA. Translation: AAH16693.1. AF176070 mRNA. Translation: AAG15840.1. AF176071 mRNA. Translation: AAG15841.1.
IPI	IPI00102599. IPI00220777. IPI00220778. IPI00220779. IPI00220780.
RefSeq	NP_620591.3. NM_139022.2.
UniGene	Hs.271954.
3D structure databases
ProteinModelPortal	Q96QS1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000182290.
Polymorphism databases
DMDM	20139069.
Proteomic databases
PaxDb	Q96QS1.
PRIDE	Q96QS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000182290; ENSP00000182290; ENSG00000064201. ENST00000381121; ENSP00000370513; ENSG00000064201. ENST00000446063; ENSP00000395018; ENSG00000064201.
GeneID	10077.
KEGG	hsa:10077.
UCSC	uc001lvx.1. human. uc001lvy.1. human.
Organism-specific databases
CTD	10077.
GeneCards	GC11P002323.
HGNC	HGNC:13410. TSPAN32.
HPA	HPA024135.
MIM	603853. gene.
neXtProt	NX_Q96QS1.
PharmGKB	PA33257.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG41704.
HOGENOM	HOG000081817.
HOVERGEN	HBG053580.
InParanoid	Q96QS1.
OMA	IRSFLRT.
PhylomeDB	Q96QS1.
Gene expression databases
ArrayExpress	Q96QS1.
Bgee	Q96QS1.
CleanEx	HS_TSPAN32.
Genevestigator	Q96QS1.
GermOnline	ENSG00000064201. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	10077.
NextBio	38097.
SOURCE	Search...

Entry information

Entry name

TSN32_HUMAN

Accession

Primary (citable) accession number: Q96QS1
Secondary accession number(s): Q96KX4 , Q9HC50, Q9HC51, Q9Y5U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.