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Protein

Tetraspanin-32

Gene

TSPAN32

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Biological processi

  1. cell-cell signaling Source: UniProtKB
  2. cytoskeleton organization Source: Ensembl
  3. defense response to protozoan Source: Ensembl
  4. integrin-mediated signaling pathway Source: Ensembl
  5. negative regulation of cell proliferation Source: Ensembl
  6. negative regulation of myeloid dendritic cell activation Source: Ensembl
  7. platelet aggregation Source: Ensembl
  8. regulation of defense response to virus Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraspanin-32
Short name:
Tspan-32
Alternative name(s):
Protein Phemx
Gene namesi
Name:TSPAN32
Synonyms:PHEMX, TSSC6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:13410. TSPAN32.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence AnalysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. integrin alphaIIb-beta3 complex Source: Ensembl
  3. intracellular Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33257.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Tetraspanin-32PRO_0000219278Add
BLAST

Proteomic databases

PaxDbiQ96QS1.
PRIDEiQ96QS1.

Expressioni

Tissue specificityi

Expressed ubiquitously at low levels. High levels of expression are confined to hematopoietic tissues including peripheral blood leukocytes, thymus and spleen.1 Publication

Developmental stagei

Expressed from early embryogenesis through to adulthood.1 Publication

Gene expression databases

BgeeiQ96QS1.
CleanExiHS_TSPAN32.
ExpressionAtlasiQ96QS1. baseline and differential.
GenevestigatoriQ96QS1.

Organism-specific databases

HPAiHPA024135.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000182290.

Structurei

3D structure databases

ProteinModelPortaliQ96QS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41704.
GeneTreeiENSGT00390000003287.
HOGENOMiHOG000081817.
HOVERGENiHBG053580.
InParanoidiQ96QS1.
KOiK17359.
OMAiAARQDCL.
PhylomeDBiQ96QS1.
TreeFamiTF336277.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
SUPFAMiSSF48652. SSF48652. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q96QS1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE
60 70 80 90 100
KNPYQAVHQW AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL
110 120 130 140 150
AFCAQVQVVF WRLHSPTQVE DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ
160 170 180 190 200
DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR EDCLQGIRSF LRTHQQVASS
210 220 230 240 250
LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR ACGRQPQEPS
260 270 280 290 300
LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR
310 320
ALQGRSRGGL SGCPERGLSD
Length:320
Mass (Da):34,631
Last modified:December 1, 2001 - v1
Checksum:i534E4C29767B8E6D
GO
Isoform 2 (identifier: Q96QS1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:290
Mass (Da):31,308
Checksum:iB9E86C72A6C9B727
GO
Isoform 3 (identifier: Q96QS1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-253: ACGRQPQEPSLLR → SPGQKSRWAQWVP
     254-320: Missing.

Show »
Length:253
Mass (Da):27,844
Checksum:iA616383C45BE67E6
GO
Isoform 4 (identifier: Q96QS1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     137-154: GTSHVRRQELAAIQDVFL → VSVLWEEVSFQPSGEHRG
     155-320: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:124
Mass (Da):13,643
Checksum:i29B8FF48896D8BB8
GO
Isoform 5 (identifier: Q96QS1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-258: VSALLFSSFL...PSLLRCSQGG → LGPQGQIHPD...SCYWSKRMLG
     259-320: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:258
Mass (Da):28,211
Checksum:i4564FAB7C7C2E9F4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3030Missing in isoform 2 and isoform 4. 2 PublicationsVSP_003932Add
BLAST
Alternative sequencei137 – 15418GTSHV…QDVFL → VSVLWEEVSFQPSGEHRG in isoform 4. 1 PublicationVSP_003933Add
BLAST
Alternative sequencei155 – 320166Missing in isoform 4. 1 PublicationVSP_003934Add
BLAST
Alternative sequencei210 – 25849VSALL…CSQGG → LGPQGQIHPDPTSMWPPAPG AQPLEMLPGWTHTLSPLRSS CYWSKRMLG in isoform 5. 1 PublicationVSP_003935Add
BLAST
Alternative sequencei241 – 25313ACGRQ…PSLLR → SPGQKSRWAQWVP in isoform 3. 1 PublicationVSP_003937Add
BLAST
Alternative sequencei254 – 32067Missing in isoform 3. 1 PublicationVSP_003938Add
BLAST
Alternative sequencei259 – 32062Missing in isoform 5. 1 PublicationVSP_003936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125569 mRNA. Translation: AAD23580.1.
AY039001 mRNA. Translation: AAK84431.1.
BC016693 mRNA. Translation: AAH16693.1.
AF176070 mRNA. Translation: AAG15840.1.
AF176071 mRNA. Translation: AAG15841.1.
CCDSiCCDS7733.1. [Q96QS1-1]
RefSeqiNP_620591.3. NM_139022.2. [Q96QS1-1]
UniGeneiHs.271954.

Genome annotation databases

EnsembliENST00000182290; ENSP00000182290; ENSG00000064201. [Q96QS1-1]
ENST00000381121; ENSP00000370513; ENSG00000064201. [Q96QS1-3]
ENST00000446063; ENSP00000395018; ENSG00000064201. [Q96QS1-5]
GeneIDi10077.
KEGGihsa:10077.
UCSCiuc001lvx.1. human. [Q96QS1-3]
uc001lvy.1. human. [Q96QS1-1]

Polymorphism databases

DMDMi20139069.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125569 mRNA. Translation: AAD23580.1.
AY039001 mRNA. Translation: AAK84431.1.
BC016693 mRNA. Translation: AAH16693.1.
AF176070 mRNA. Translation: AAG15840.1.
AF176071 mRNA. Translation: AAG15841.1.
CCDSiCCDS7733.1. [Q96QS1-1]
RefSeqiNP_620591.3. NM_139022.2. [Q96QS1-1]
UniGeneiHs.271954.

3D structure databases

ProteinModelPortaliQ96QS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000182290.

Polymorphism databases

DMDMi20139069.

Proteomic databases

PaxDbiQ96QS1.
PRIDEiQ96QS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000182290; ENSP00000182290; ENSG00000064201. [Q96QS1-1]
ENST00000381121; ENSP00000370513; ENSG00000064201. [Q96QS1-3]
ENST00000446063; ENSP00000395018; ENSG00000064201. [Q96QS1-5]
GeneIDi10077.
KEGGihsa:10077.
UCSCiuc001lvx.1. human. [Q96QS1-3]
uc001lvy.1. human. [Q96QS1-1]

Organism-specific databases

CTDi10077.
GeneCardsiGC11P002323.
HGNCiHGNC:13410. TSPAN32.
HPAiHPA024135.
MIMi603853. gene.
neXtProtiNX_Q96QS1.
PharmGKBiPA33257.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41704.
GeneTreeiENSGT00390000003287.
HOGENOMiHOG000081817.
HOVERGENiHBG053580.
InParanoidiQ96QS1.
KOiK17359.
OMAiAARQDCL.
PhylomeDBiQ96QS1.
TreeFamiTF336277.

Miscellaneous databases

ChiTaRSiTSPAN32. human.
GeneWikiiTSPAN32.
GenomeRNAii10077.
NextBioi38097.
PROiQ96QS1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96QS1.
CleanExiHS_TSPAN32.
ExpressionAtlasiQ96QS1. baseline and differential.
GenevestigatoriQ96QS1.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
SUPFAMiSSF48652. SSF48652. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting."
    Lee M.P., Brandenburg S., Landes G.M., Adams M., Miller G., Feinberg A.P.
    Hum. Mol. Genet. 8:683-690(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
  2. "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs."
    Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B., Wright M.D.
    Biochim. Biophys. Acta 1522:31-41(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    Tissue: Lung.
  4. "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7."
    Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J., Hughes M.R., Ko M.S.H.
    Genomics 68:13-21(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-320 (ISOFORMS 3 AND 4), DEVELOPMENTAL STAGE.
  5. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).

Entry informationi

Entry nameiTSN32_HUMAN
AccessioniPrimary (citable) accession number: Q96QS1
Secondary accession number(s): Q96KX4
, Q9HC50, Q9HC51, Q9Y5U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.