Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96QS1 (TSN32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tetraspanin-32

Short name=Tspan-32
Alternative name(s):
Protein Phemx
Gene names
Name:TSPAN32
Synonyms:PHEMX, TSSC6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed ubiquitously at low levels. High levels of expression are confined to hematopoietic tissues including peripheral blood leukocytes, thymus and spleen. Ref.1

Developmental stage

Expressed from early embryogenesis through to adulthood. Ref.4

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q96QS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96QS1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
Isoform 3 (identifier: Q96QS1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     241-253: ACGRQPQEPSLLR → SPGQKSRWAQWVP
     254-320: Missing.
Isoform 4 (identifier: Q96QS1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     137-154: GTSHVRRQELAAIQDVFL → VSVLWEEVSFQPSGEHRG
     155-320: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 5 (identifier: Q96QS1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     210-258: VSALLFSSFL...PSLLRCSQGG → LGPQGQIHPD...SCYWSKRMLG
     259-320: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320Tetraspanin-32
PRO_0000219278

Regions

Transmembrane14 – 3421Helical; Potential
Transmembrane60 – 8021Helical; Potential
Transmembrane90 – 11021Helical; Potential
Transmembrane203 – 22321Helical; Potential

Natural variations

Alternative sequence1 – 3030Missing in isoform 2 and isoform 4.
VSP_003932
Alternative sequence137 – 15418GTSHV…QDVFL → VSVLWEEVSFQPSGEHRG in isoform 4.
VSP_003933
Alternative sequence155 – 320166Missing in isoform 4.
VSP_003934
Alternative sequence210 – 25849VSALL…CSQGG → LGPQGQIHPDPTSMWPPAPG AQPLEMLPGWTHTLSPLRSS CYWSKRMLG in isoform 5.
VSP_003935
Alternative sequence241 – 25313ACGRQ…PSLLR → SPGQKSRWAQWVP in isoform 3.
VSP_003937
Alternative sequence254 – 32067Missing in isoform 3.
VSP_003938
Alternative sequence259 – 32062Missing in isoform 5.
VSP_003936

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 534E4C29767B8E6D

FASTA32034,631
        10         20         30         40         50         60 
MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW 

        70         80         90        100        110        120 
AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE 

       130        140        150        160        170        180 
DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR 

       190        200        210        220        230        240 
EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR 

       250        260        270        280        290        300 
ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR 

       310        320 
ALQGRSRGGL SGCPERGLSD 

« Hide

Isoform 2 [UniParc].

Checksum: B9E86C72A6C9B727
Show »

FASTA29031,308
Isoform 3 [UniParc].

Checksum: A616383C45BE67E6
Show »

FASTA25327,844
Isoform 4 [UniParc].

Checksum: 29B8FF48896D8BB8
Show »

FASTA12413,643
Isoform 5 [UniParc].

Checksum: 4564FAB7C7C2E9F4
Show »

FASTA25828,211

References

« Hide 'large scale' references
[1]"Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting."
Lee M.P., Brandenburg S., Landes G.M., Adams M., Miller G., Feinberg A.P.
Hum. Mol. Genet. 8:683-690(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[2]"Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs."
Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B., Wright M.D.
Biochim. Biophys. Acta 1522:31-41(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
Tissue: Lung.
[4]"Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7."
Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J., Hughes M.R., Ko M.S.H.
Genomics 68:13-21(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-320 (ISOFORMS 3 AND 4), DEVELOPMENTAL STAGE.
[5]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF125569 mRNA. Translation: AAD23580.1.
AY039001 mRNA. Translation: AAK84431.1.
BC016693 mRNA. Translation: AAH16693.1.
AF176070 mRNA. Translation: AAG15840.1.
AF176071 mRNA. Translation: AAG15841.1.
CCDSCCDS7733.1. [Q96QS1-1]
RefSeqNP_620591.3. NM_139022.2. [Q96QS1-1]
UniGeneHs.271954.

3D structure databases

ProteinModelPortalQ96QS1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000182290.

Polymorphism databases

DMDM20139069.

Proteomic databases

PaxDbQ96QS1.
PRIDEQ96QS1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000182290; ENSP00000182290; ENSG00000064201. [Q96QS1-1]
ENST00000381121; ENSP00000370513; ENSG00000064201. [Q96QS1-3]
ENST00000446063; ENSP00000395018; ENSG00000064201. [Q96QS1-5]
GeneID10077.
KEGGhsa:10077.
UCSCuc001lvx.1. human. [Q96QS1-3]
uc001lvy.1. human. [Q96QS1-1]

Organism-specific databases

CTD10077.
GeneCardsGC11P002323.
HGNCHGNC:13410. TSPAN32.
HPAHPA024135.
MIM603853. gene.
neXtProtNX_Q96QS1.
PharmGKBPA33257.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG41704.
HOGENOMHOG000081817.
HOVERGENHBG053580.
InParanoidQ96QS1.
KOK17359.
OMAAARQDCL.
PhylomeDBQ96QS1.
TreeFamTF336277.

Gene expression databases

ArrayExpressQ96QS1.
BgeeQ96QS1.
CleanExHS_TSPAN32.
GenevestigatorQ96QS1.

Family and domain databases

Gene3D1.10.1450.10. 1 hit.
InterProIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
SUPFAMSSF48652. SSF48652. 1 hit.
ProtoNetSearch...

Other

GeneWikiTSPAN32.
GenomeRNAi10077.
NextBio38097.
PROQ96QS1.
SOURCESearch...

Entry information

Entry nameTSN32_HUMAN
AccessionPrimary (citable) accession number: Q96QS1
Secondary accession number(s): Q96KX4 expand/collapse secondary AC list , Q9HC50, Q9HC51, Q9Y5U1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM