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Q96QP1 (ALPK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-protein kinase 1

EC=2.7.11.-
Alternative name(s):
Chromosome 4 kinase
Lymphocyte alpha-protein kinase
Gene names
Name:ALPK1
Synonyms:KIAA1527, LAK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1244 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.

Tissue specificity

Highly expressed in liver. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

Contains 1 alpha-type protein kinase domain.

Sequence caution

The sequence BAC85140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC85140.1 differs from that shown. Reason: Frameshift at positions 208 and 1022.

The sequence BAC85140.1 differs from that shown. Reason: Intron retention.

The sequence BC060780 differs from that shown. Reason: Frameshift at position 1044.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96QP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96QP1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: MNNQKVVAVL...VIGAGLQQLL → MCRKRTRARTSAAE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12441244Alpha-protein kinase 1
PRO_0000260028

Regions

Domain1017 – 1237221Alpha-type protein kinase
Compositional bias105 – 1095Poly-Ala
Compositional bias921 – 96040Ser-rich

Natural variations

Alternative sequence1 – 9292MNNQK…LQQLL → MCRKRTRARTSAAE in isoform 2.
VSP_044735
Natural variant671Q → R. Ref.9
Corresponds to variant rs33943680 [ dbSNP | Ensembl ].
VAR_041511
Natural variant1751N → D. Ref.9
Corresponds to variant rs6533616 [ dbSNP | Ensembl ].
VAR_028982
Natural variant2921T → M. Ref.9
Corresponds to variant rs34120296 [ dbSNP | Ensembl ].
VAR_041512
Natural variant3201L → M. Ref.9
VAR_041513
Natural variant3391K → E in an ovarian mucinous carcinoma sample; somatic mutation. Ref.9
VAR_041514
Natural variant3831K → E. Ref.9
Corresponds to variant rs147641444 [ dbSNP | Ensembl ].
VAR_041515
Natural variant5651G → D. Ref.1 Ref.2 Ref.5 Ref.6 Ref.9
Corresponds to variant rs2074388 [ dbSNP | Ensembl ].
VAR_028983
Natural variant6421H → R. Ref.1 Ref.2 Ref.5 Ref.6 Ref.9
Corresponds to variant rs13148353 [ dbSNP | Ensembl ].
VAR_028984
Natural variant6601P → L. Ref.9
Corresponds to variant rs35389530 [ dbSNP | Ensembl ].
VAR_041516
Natural variant6811G → D. Ref.2 Ref.9
Corresponds to variant rs35519493 [ dbSNP | Ensembl ].
VAR_041517
Natural variant7321M → I. Ref.1 Ref.2 Ref.5 Ref.6 Ref.9
Corresponds to variant rs2074379 [ dbSNP | Ensembl ].
VAR_028985
Natural variant8611M → T. Ref.1 Ref.2 Ref.5 Ref.6 Ref.9
Corresponds to variant rs11726117 [ dbSNP | Ensembl ].
VAR_028986
Natural variant8701G → S. Ref.9
Corresponds to variant rs2074380 [ dbSNP | Ensembl ].
VAR_028987
Natural variant8731R → I. Ref.9
Corresponds to variant rs34946272 [ dbSNP | Ensembl ].
VAR_041518
Natural variant9101E → D. Ref.9
Corresponds to variant rs35308602 [ dbSNP | Ensembl ].
VAR_041519
Natural variant9161N → D. Ref.9
Corresponds to variant rs2074381 [ dbSNP | Ensembl ].
VAR_028988
Natural variant9351P → L. Ref.9
Corresponds to variant rs34780600 [ dbSNP | Ensembl ].
VAR_041520
Natural variant10081H → P.
Corresponds to variant rs34079946 [ dbSNP | Ensembl ].
VAR_057741
Natural variant10841R → Q. Ref.9
Corresponds to variant rs34677416 [ dbSNP | Ensembl ].
VAR_041521
Natural variant11171L → P. Ref.9
Corresponds to variant rs35756863 [ dbSNP | Ensembl ].
VAR_041522
Natural variant11601A → G. Ref.9
Corresponds to variant rs55696324 [ dbSNP | Ensembl ].
VAR_041523

Experimental info

Sequence conflict831V → L in BAC85140. Ref.5
Sequence conflict5771S → G in BAG65473. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 857D353D159A1630

FASTA1,244138,861
        10         20         30         40         50         60 
MNNQKVVAVL LQECKQVLDQ LLLEAPDVSE EDKSEDQRCR ALLPSELRTL IQEAKEMKWP 

        70         80         90        100        110        120 
FVPEKWQYKQ AVGPEDKTNL KDVIGAGLQQ LLASLRASIL ARDCAAAAAI VFLVDRFLYG 

       130        140        150        160        170        180 
LDVSGKLLQV AKGLHKLQPA TPIAPQVVIR QARISVNSGK LLKAEYILSS LISNNGATGT 

       190        200        210        220        230        240 
WLYRNESDKV LVQSVCIQIR GQILQKLGMW YEAAELIWAS IVGYLALPQP DKKGLSTSLG 

       250        260        270        280        290        300 
ILADIFVSMS KNDYEKFKNN PQINLSLLKE FDHHLLSAAE ACKLAAAFSA YTPLFVLTAV 

       310        320        330        340        350        360 
NIRGTCLLSY SSSNDCPPEL KNLHLCEAKE AFEIGLLTKR DDEPVTGKQE LHSFVKAAFG 

       370        380        390        400        410        420 
LTTVHRRLHG ETGTVHAASQ LCKEAMGKLY NFSTSSRSQD REALSQEVMS VIAQVKEHLQ 

       430        440        450        460        470        480 
VQSFSNVDDR SYVPESFECR LDKLILHGQG DFQKILDTYS QHHTSVCEVF ESDCGNNKNE 

       490        500        510        520        530        540 
QKDAKTGVCI TALKTEIKNI DTVSTTQEKP HCQRDTGISS SLMGKNVQRE LRRGGRRNWT 

       550        560        570        580        590        600 
HSDAFRVSLD QDVETETEPS DYSNGEGAVF NKSLSGSQTS SAWSNLSGFS SSASWEEVNY 

       610        620        630        640        650        660 
HVDDRSARKE PGKEHLVDTQ CSTALSEELE NDREGRAMHS LHSQLHDLSL QEPNNDNLEP 

       670        680        690        700        710        720 
SQNQPQQQMP LTPFSPHNTP GIFLAPGAGL LEGAPEGIQE VRNMGPRNTS AHSRPSYRSA 

       730        740        750        760        770        780 
SWSSDSGRPK NMGTHPSVQK EEAFEIIVEF PETNCDVKDR QGKEQGEEIS ERGAGPTFKA 

       790        800        810        820        830        840 
SPSWVDPEGE TAESTEDAPL DFHRVLHNSL GNISMLPCSS FTPNWPVQNP DSRKSGGPVA 

       850        860        870        880        890        900 
EQGIDPDAST VDEEGQLLDS MDVPCTNGHG SHRLCILRQP PGQRAETPNS SVSGNILFPV 

       910        920        930        940        950        960 
LSEDCTTTEE GNQPGNMLNC SQNSSSSSVW WLKSPAFSSG SSEGDSPWSY LNSSGSSWVS 

       970        980        990       1000       1010       1020 
LPGKMRKEIL EARTLQPDDF EKLLAGVRHD WLFQRLENTG VFKPSQLHRA HSALLLKYSK 

      1030       1040       1050       1060       1070       1080 
KSELWTAQET IVYLGDYLTV KKKGRQRNAF WVHHLHQEEI LGRYVGKDYK EQKGLWHHFT 

      1090       1100       1110       1120       1130       1140 
DVERQMTAQH YVTEFNKRLY EQNIPTQIFY IPSTILLILE DKTIKGCISV EPYILGEFVK 

      1150       1160       1170       1180       1190       1200 
LSNNTKVVKT EYKATEYGLA YGHFSYEFSN HRDVVVDLQG WVTGNGKGLI YLTDPQIHSV 

      1210       1220       1230       1240 
DQKVFTTNFG KRGIFYFFNN QHVECNEICH RLSLTRPSME KPCT 

« Hide

Isoform 2 [UniParc].

Checksum: 1996AB197A4DDEEC
Show »

FASTA1,166129,951

References

« Hide 'large scale' references
[1]"Alpha-kinases: a new class of protein kinases with a novel catalytic domain."
Ryazanov A.G., Pavur K.S., Dorovkov M.V.
Curr. Biol. 9:R43-R45(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ASP-565; ARG-642; ASP-681; ILE-732 AND THR-861.
Tissue: Uterus.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[5]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 77-1244 (ISOFORM 1), VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861.
Tissue: Spleen.
[6]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 444-1244 (ISOFORM 1), VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861, TISSUE SPECIFICITY.
Tissue: Brain.
[7]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[8]"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1018-1244 (ISOFORM 1).
[9]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-67; ASP-175; MET-292; MET-320; GLU-339; GLU-383; ASP-565; ARG-642; LEU-660; ASP-681; ILE-732; THR-861; SER-870; ILE-873; ASP-910; ASP-916; LEU-935; GLN-1084; PRO-1117 AND GLY-1160.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY044164 mRNA. Translation: AAK94675.1.
AK304708 mRNA. Translation: BAG65473.1.
AC004049 Genomic DNA. No translation available.
AC109347 Genomic DNA. No translation available.
BC060780 mRNA. No translation available.
AK131090 mRNA. Translation: BAC85140.1. Sequence problems.
AB040960 mRNA. Translation: BAA96051.1.
AB075877 mRNA. Translation: BAD38659.1.
CCDSCCDS3697.1. [Q96QP1-1]
CCDS58923.1. [Q96QP1-2]
RefSeqNP_001095876.1. NM_001102406.1. [Q96QP1-1]
NP_001240813.1. NM_001253884.1. [Q96QP1-2]
NP_079420.3. NM_025144.3. [Q96QP1-1]
UniGeneHs.652825.

3D structure databases

ProteinModelPortalQ96QP1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123183. 2 interactions.
IntActQ96QP1. 1 interaction.
STRING9606.ENSP00000177648.

PTM databases

PhosphoSiteQ96QP1.

Polymorphism databases

DMDM308153632.

Proteomic databases

PaxDbQ96QP1.
PRIDEQ96QP1.

Protocols and materials databases

DNASU80216.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000177648; ENSP00000177648; ENSG00000073331. [Q96QP1-1]
ENST00000458497; ENSP00000398048; ENSG00000073331. [Q96QP1-1]
ENST00000504176; ENSP00000426044; ENSG00000073331. [Q96QP1-2]
GeneID80216.
KEGGhsa:80216.
UCSCuc003ian.4. human. [Q96QP1-1]

Organism-specific databases

CTD80216.
GeneCardsGC04P113218.
H-InvDBHIX0004446.
HGNCHGNC:20917. ALPK1.
HPAHPA027435.
HPA027443.
MIM607347. gene.
neXtProtNX_Q96QP1.
PharmGKBPA134891785.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG73232.
HOGENOMHOG000169279.
HOVERGENHBG079770.
InParanoidQ96QP1.
KOK08868.
OMANIRGTCL.
OrthoDBEOG76QFGG.
PhylomeDBQ96QP1.
TreeFamTF316085.

Gene expression databases

ArrayExpressQ96QP1.
BgeeQ96QP1.
CleanExHS_ALPK1.
GenevestigatorQ96QP1.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
[Graphical view]
PfamPF02816. Alpha_kinase. 1 hit.
[Graphical view]
SMARTSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiALPK1.
GenomeRNAi80216.
NextBio70616.
PROQ96QP1.
SOURCESearch...

Entry information

Entry nameALPK1_HUMAN
AccessionPrimary (citable) accession number: Q96QP1
Secondary accession number(s): B4E3G1 expand/collapse secondary AC list , F5H138, Q68CI9, Q6P9F9, Q6ZNK4, Q9P201
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 5, 2010
Last modified: July 9, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM