Q96QP1 (ALPK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-protein kinase 1
EC=2.7.11.-

Alternative name(s):
Chromosome 4 kinase
Lymphocyte alpha-protein kinase

Gene names

Name:	ALPK1
Synonyms:	KIAA1527, LAK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1244 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Tissue specificity	Highly expressed in liver. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. Contains 1 alpha-type protein kinase domain.
Sequence caution	The sequence BAC85140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC85140.1 differs from that shown. Reason: Frameshift at positions 208 and 1022. The sequence BAC85140.1 differs from that shown. Reason: Intron retention. The sequence BC060780 differs from that shown. Reason: Frameshift at position 1044.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1244	1244	Alpha-protein kinase 1		PRO_0000260028
Regions
	Domain	1017 – 1237	221	Alpha-type protein kinase
	Compositional bias	105 – 109	5	Poly-Ala
	Compositional bias	921 – 960	40	Ser-rich
Natural variations
	Natural variant	67	1	Q → R. Ref.8 Corresponds to variant rs33943680 [ dbSNP | Ensembl ].		VAR_041511
	Natural variant	175	1	N → D. Ref.8 Corresponds to variant rs6533616 [ dbSNP | Ensembl ].		VAR_028982
	Natural variant	292	1	T → M. Ref.8 Corresponds to variant rs34120296 [ dbSNP | Ensembl ].		VAR_041512
	Natural variant	320	1	L → M. Ref.8		VAR_041513
	Natural variant	339	1	K → E in an ovarian mucinous carcinoma sample; somatic mutation. Ref.8		VAR_041514
	Natural variant	383	1	K → E. Ref.8		VAR_041515
	Natural variant	565	1	G → D. Ref.1 Ref.4 Ref.5 Ref.8 Corresponds to variant rs2074388 [ dbSNP | Ensembl ].		VAR_028983
	Natural variant	642	1	H → R. Ref.1 Ref.4 Ref.5 Ref.8 Corresponds to variant rs13148353 [ dbSNP | Ensembl ].		VAR_028984
	Natural variant	660	1	P → L. Ref.8 Corresponds to variant rs35389530 [ dbSNP | Ensembl ].		VAR_041516
	Natural variant	681	1	G → D. Ref.8 Corresponds to variant rs35519493 [ dbSNP | Ensembl ].		VAR_041517
	Natural variant	732	1	M → I. Ref.1 Ref.4 Ref.5 Ref.8 Corresponds to variant rs2074379 [ dbSNP | Ensembl ].		VAR_028985
	Natural variant	861	1	M → T. Ref.1 Ref.4 Ref.5 Ref.8 Corresponds to variant rs11726117 [ dbSNP | Ensembl ].		VAR_028986
	Natural variant	870	1	G → S. Ref.8 Corresponds to variant rs2074380 [ dbSNP | Ensembl ].		VAR_028987
	Natural variant	873	1	R → I. Ref.8 Corresponds to variant rs34946272 [ dbSNP | Ensembl ].		VAR_041518
	Natural variant	910	1	E → D. Ref.8 Corresponds to variant rs35308602 [ dbSNP | Ensembl ].		VAR_041519
	Natural variant	916	1	N → D. Ref.8 Corresponds to variant rs2074381 [ dbSNP | Ensembl ].		VAR_028988
	Natural variant	935	1	P → L. Ref.8 Corresponds to variant rs34780600 [ dbSNP | Ensembl ].		VAR_041520
	Natural variant	1008	1	H → P. Corresponds to variant rs34079946 [ dbSNP | Ensembl ].		VAR_057741
	Natural variant	1084	1	R → Q. Ref.8 Corresponds to variant rs34677416 [ dbSNP | Ensembl ].		VAR_041521
	Natural variant	1117	1	L → P. Ref.8 Corresponds to variant rs35756863 [ dbSNP | Ensembl ].		VAR_041522
	Natural variant	1160	1	A → G. Ref.8 Corresponds to variant rs55696324 [ dbSNP | Ensembl ].		VAR_041523
Experimental info
	Sequence conflict	83	1	V → L in BAC85140. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q96QP1 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 857D353D159A1630	FASTA	1,244	138,861
	10 20 30 40 50 60 MNNQKVVAVL LQECKQVLDQ LLLEAPDVSE EDKSEDQRCR ALLPSELRTL IQEAKEMKWP 70 80 90 100 110 120 FVPEKWQYKQ AVGPEDKTNL KDVIGAGLQQ LLASLRASIL ARDCAAAAAI VFLVDRFLYG 130 140 150 160 170 180 LDVSGKLLQV AKGLHKLQPA TPIAPQVVIR QARISVNSGK LLKAEYILSS LISNNGATGT 190 200 210 220 230 240 WLYRNESDKV LVQSVCIQIR GQILQKLGMW YEAAELIWAS IVGYLALPQP DKKGLSTSLG 250 260 270 280 290 300 ILADIFVSMS KNDYEKFKNN PQINLSLLKE FDHHLLSAAE ACKLAAAFSA YTPLFVLTAV 310 320 330 340 350 360 NIRGTCLLSY SSSNDCPPEL KNLHLCEAKE AFEIGLLTKR DDEPVTGKQE LHSFVKAAFG 370 380 390 400 410 420 LTTVHRRLHG ETGTVHAASQ LCKEAMGKLY NFSTSSRSQD REALSQEVMS VIAQVKEHLQ 430 440 450 460 470 480 VQSFSNVDDR SYVPESFECR LDKLILHGQG DFQKILDTYS QHHTSVCEVF ESDCGNNKNE 490 500 510 520 530 540 QKDAKTGVCI TALKTEIKNI DTVSTTQEKP HCQRDTGISS SLMGKNVQRE LRRGGRRNWT 550 560 570 580 590 600 HSDAFRVSLD QDVETETEPS DYSNGEGAVF NKSLSGSQTS SAWSNLSGFS SSASWEEVNY 610 620 630 640 650 660 HVDDRSARKE PGKEHLVDTQ CSTALSEELE NDREGRAMHS LHSQLHDLSL QEPNNDNLEP 670 680 690 700 710 720 SQNQPQQQMP LTPFSPHNTP GIFLAPGAGL LEGAPEGIQE VRNMGPRNTS AHSRPSYRSA 730 740 750 760 770 780 SWSSDSGRPK NMGTHPSVQK EEAFEIIVEF PETNCDVKDR QGKEQGEEIS ERGAGPTFKA 790 800 810 820 830 840 SPSWVDPEGE TAESTEDAPL DFHRVLHNSL GNISMLPCSS FTPNWPVQNP DSRKSGGPVA 850 860 870 880 890 900 EQGIDPDAST VDEEGQLLDS MDVPCTNGHG SHRLCILRQP PGQRAETPNS SVSGNILFPV 910 920 930 940 950 960 LSEDCTTTEE GNQPGNMLNC SQNSSSSSVW WLKSPAFSSG SSEGDSPWSY LNSSGSSWVS 970 980 990 1000 1010 1020 LPGKMRKEIL EARTLQPDDF EKLLAGVRHD WLFQRLENTG VFKPSQLHRA HSALLLKYSK 1030 1040 1050 1060 1070 1080 KSELWTAQET IVYLGDYLTV KKKGRQRNAF WVHHLHQEEI LGRYVGKDYK EQKGLWHHFT 1090 1100 1110 1120 1130 1140 DVERQMTAQH YVTEFNKRLY EQNIPTQIFY IPSTILLILE DKTIKGCISV EPYILGEFVK 1150 1160 1170 1180 1190 1200 LSNNTKVVKT EYKATEYGLA YGHFSYEFSN HRDVVVDLQG WVTGNGKGLI YLTDPQIHSV 1210 1220 1230 1240 DQKVFTTNFG KRGIFYFFNN QHVECNEICH RLSLTRPSME KPCT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alpha-kinases: a new class of protein kinases with a novel catalytic domain." Ryazanov A.G., Pavur K.S., Dorovkov M.V. Curr. Biol. 9:R43-R45(1999) [PubMed: 10021370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 77-1244, VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861. Tissue: Spleen.
[5]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 444-1244, VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861, TISSUE SPECIFICITY. Tissue: Brain.
[6]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[7]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed: 15221005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1018-1244.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-67; ASP-175; MET-292; MET-320; GLU-339; GLU-383; ASP-565; ARG-642; LEU-660; ASP-681; ILE-732; THR-861; SER-870; ILE-873; ASP-910; ASP-916; LEU-935; GLN-1084; PRO-1117 AND GLY-1160.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY044164 mRNA. Translation: AAK94675.1. AC004049 Genomic DNA. No translation available. BC060780 mRNA. No translation available. AK131090 mRNA. Translation: BAC85140.1. Sequence problems. AB040960 mRNA. Translation: BAA96051.1. AB075877 mRNA. Translation: BAD38659.1.
IPI	IPI00304706.
RefSeq	NP_001095876.1. NM_001102406.1. NP_079420.3. NM_025144.3.
UniGene	Hs.652825.
3D structure databases
HSSP	HSSP built from PDB template 1IA9 based on UniProtKB Q923J1.
ProteinModelPortal	Q96QP1.
SMR	Q96QP1. Positions 1012-1240.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96QP1.
Polymorphism databases
DMDM	308153632.
Proteomic databases
PRIDE	Q96QP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000177648; ENSP00000177648; ENSG00000073331. ENST00000458497; ENSP00000398048; ENSG00000073331.
GeneID	80216.
KEGG	hsa:80216.
UCSC	uc003ian.2. human.
Organism-specific databases
CTD	80216.
GeneCards	GC04P113218.
H-InvDB	HIX0004446.
HGNC	HGNC:20917. ALPK1.
HPA	HPA027435. HPA027443.
MIM	607347. gene.
neXtProt	NX_Q96QP1.
PharmGKB	PA134891785.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07561.
GeneTree	ENSGT00530000063613.
HOGENOM	HBG446763.
HOVERGEN	HBG079770.
InParanoid	Q96QP1.
OMA	ADIFVSM.
OrthoDB	EOG4QNMVF.
PhylomeDB	Q96QP1.
Gene expression databases
ArrayExpress	Q96QP1.
Bgee	Q96QP1.
CleanEx	HS_ALPK1.
Genevestigator	Q96QP1.
GermOnline	ENSG00000073331. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR004166. MHCK_EF2_kinase. [Graphical view]
KO	K08868.
Pfam	PF02816. Alpha_kinase. 1 hit. [Graphical view]
SMART	SM00811. Alpha_kinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51158. ALPHA_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	70616.
SOURCE	Search...

Entry information

Entry name

ALPK1_HUMAN

Accession

Primary (citable) accession number: Q96QP1
Secondary accession number(s): Q68CI9 , Q6P9F9, Q6ZNK4, Q9P201

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 5, 2010
Last modified:	January 25, 2012
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.