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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 6

Gene

HS3ST6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to heparan sulfate. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes Simplex Virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181PAPSBy similarity1
Binding sitei189PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 104PAPSBy similarity5
Nucleotide bindingi305 – 309PAPSBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:HS08637-MONOMER.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 6 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 6
Short name:
3-OST-6
Short name:
Heparan sulfate 3-O-sulfotransferase 6
Short name:
h3-OST-6
Gene namesi
Name:HS3ST6
Synonyms:HS3ST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14178. HS3ST6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 49Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini50 – 342LumenalSequence analysisAdd BLAST293

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi64711.
OpenTargetsiENSG00000162040.
ENSG00000276333.

Polymorphism and mutation databases

DMDMi61214397.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852241 – 342Heparan sulfate glucosamine 3-O-sulfotransferase 6Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi288 ↔ 300By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96QI5.
PRIDEiQ96QI5.

PTM databases

iPTMnetiQ96QI5.
PhosphoSitePlusiQ96QI5.

Expressioni

Gene expression databases

BgeeiENSG00000162040.
CleanExiHS_HS3ST5.
HS_HS3ST6.
ExpressionAtlasiQ96QI5. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000390354.

Structurei

3D structure databases

ProteinModelPortaliQ96QI5.
SMRiQ96QI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 128Substrate bindingBy similarity7
Regioni153 – 156Substrate bindingBy similarity4
Regioni220 – 221Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ96QI5.
KOiK09679.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ96QI5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96QI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSGGLGGG AGGGQGAGAG QGAALRASRA PMLLVALVLG AYCLCALPGR
60 70 80 90 100
CPPAARAPAP APAPSEPSSS VHRPGAPGLP LASGPGRRRF PQALIVGVKK
110 120 130 140 150
GGTRALLEFL RLHPDVRALG SEPHFFDRCY ERGLAWYRSL MPRTLDGQIT
160 170 180 190 200
MEKTPSYFVT REAPRRIHAM SPDTKLIVVV RNPVTRAISD YAQTLSKTPG
210 220 230 240 250
LPSFRALAFR HGLGPVDTAW SAVRIGLYAQ HLDHWLRYFP LSHFLFVSGE
260 270 280 290 300
RLVSDPAGEV GRVQDFLGLK RVVTDKHFYF NATKGFPCLK KAQGGSRPRC
310 320 330 340
LGKSKGRPHP RVPQALVRRL QEFYRPFNRR FYQMTGQDFG WG
Length:342
Mass (Da):37,186
Last modified:March 15, 2005 - v2
Checksum:i3CB8FFD4AFA7F9DC
GO

Sequence cautioni

The sequence AAK61299 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAC42157 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192A → T (PubMed:11157797).Curated1
Sequence conflicti316L → V (PubMed:11157797).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006640 Genomic DNA. Translation: AAK61299.1. Different initiation.
AL031723 Genomic DNA. Translation: CAC42157.1. Different initiation.
CCDSiCCDS45381.2.
RefSeqiNP_001009606.3. NM_001009606.3.
UniGeneiHs.670144.

Genome annotation databases

EnsembliENST00000293937; ENSP00000293937; ENSG00000276333.
ENST00000454677; ENSP00000416741; ENSG00000162040.
GeneIDi64711.
KEGGihsa:64711.
UCSCiuc032epu.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006640 Genomic DNA. Translation: AAK61299.1. Different initiation.
AL031723 Genomic DNA. Translation: CAC42157.1. Different initiation.
CCDSiCCDS45381.2.
RefSeqiNP_001009606.3. NM_001009606.3.
UniGeneiHs.670144.

3D structure databases

ProteinModelPortaliQ96QI5.
SMRiQ96QI5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000390354.

PTM databases

iPTMnetiQ96QI5.
PhosphoSitePlusiQ96QI5.

Polymorphism and mutation databases

DMDMi61214397.

Proteomic databases

PaxDbiQ96QI5.
PRIDEiQ96QI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293937; ENSP00000293937; ENSG00000276333.
ENST00000454677; ENSP00000416741; ENSG00000162040.
GeneIDi64711.
KEGGihsa:64711.
UCSCiuc032epu.2. human.

Organism-specific databases

CTDi64711.
DisGeNETi64711.
GeneCardsiHS3ST6.
HGNCiHGNC:14178. HS3ST6.
neXtProtiNX_Q96QI5.
OpenTargetsiENSG00000162040.
ENSG00000276333.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ96QI5.
KOiK09679.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ96QI5.

Enzyme and pathway databases

BioCyciZFISH:HS08637-MONOMER.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Miscellaneous databases

GenomeRNAii64711.
PROiQ96QI5.

Gene expression databases

BgeeiENSG00000162040.
CleanExiHS_HS3ST5.
HS_HS3ST6.
ExpressionAtlasiQ96QI5. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHS3S6_HUMAN
AccessioniPrimary (citable) accession number: Q96QI5
Secondary accession number(s): Q96RX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.