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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 6

Gene

HS3ST6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to heparan sulfate. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes Simplex Virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181PAPSBy similarity1
Binding sitei189PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 104PAPSBy similarity5
Nucleotide bindingi305 – 309PAPSBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-HSA-2022928 HS-GAG biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 6 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 6
Short name:
3-OST-6
Short name:
Heparan sulfate 3-O-sulfotransferase 6
Short name:
h3-OST-6
Gene namesi
Name:HS3ST6
Synonyms:HS3ST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000162040.6
HGNCiHGNC:14178 HS3ST6
neXtProtiNX_Q96QI5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 49Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini50 – 342LumenalSequence analysisAdd BLAST293

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi64711
OpenTargetsiENSG00000162040

Polymorphism and mutation databases

DMDMi61214397

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852241 – 342Heparan sulfate glucosamine 3-O-sulfotransferase 6Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi281N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi288 ↔ 300By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96QI5
PeptideAtlasiQ96QI5
PRIDEiQ96QI5

PTM databases

iPTMnetiQ96QI5
PhosphoSitePlusiQ96QI5

Expressioni

Gene expression databases

BgeeiENSG00000162040
CleanExiHS_HS3ST5
HS_HS3ST6

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000390354

Structurei

3D structure databases

ProteinModelPortaliQ96QI5
SMRiQ96QI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 128Substrate bindingBy similarity7
Regioni153 – 156Substrate bindingBy similarity4
Regioni220 – 221Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiQ96QI5
KOiK09679
OMAiCDSAPRT
OrthoDBiEOG091G0CS5
PhylomeDBiQ96QI5

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

Q96QI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSGGLGGG AGGGQGAGAG QGAALRASRA PMLLVALVLG AYCLCALPGR
60 70 80 90 100
CPPAARAPAP APAPSEPSSS VHRPGAPGLP LASGPGRRRF PQALIVGVKK
110 120 130 140 150
GGTRALLEFL RLHPDVRALG SEPHFFDRCY ERGLAWYRSL MPRTLDGQIT
160 170 180 190 200
MEKTPSYFVT REAPRRIHAM SPDTKLIVVV RNPVTRAISD YAQTLSKTPG
210 220 230 240 250
LPSFRALAFR HGLGPVDTAW SAVRIGLYAQ HLDHWLRYFP LSHFLFVSGE
260 270 280 290 300
RLVSDPAGEV GRVQDFLGLK RVVTDKHFYF NATKGFPCLK KAQGGSRPRC
310 320 330 340
LGKSKGRPHP RVPQALVRRL QEFYRPFNRR FYQMTGQDFG WG
Length:342
Mass (Da):37,186
Last modified:March 15, 2005 - v2
Checksum:i3CB8FFD4AFA7F9DC
GO

Sequence cautioni

The sequence AAK61299 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAC42157 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192A → T (PubMed:11157797).Curated1
Sequence conflicti316L → V (PubMed:11157797).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006640 Genomic DNA Translation: AAK61299.1 Different initiation.
AL031723 Genomic DNA Translation: CAC42157.1 Different initiation.
CCDSiCCDS45381.2
RefSeqiNP_001009606.3, NM_001009606.3
UniGeneiHs.670144

Genome annotation databases

EnsembliENST00000293937; ENSP00000293937; ENSG00000276333
ENST00000454677; ENSP00000416741; ENSG00000162040
GeneIDi64711
KEGGihsa:64711
UCSCiuc032epu.2 human

Similar proteinsi

Entry informationi

Entry nameiHS3S6_HUMAN
AccessioniPrimary (citable) accession number: Q96QI5
Secondary accession number(s): Q96RX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: December 20, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health