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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 6

Gene

HS3ST6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to heparan sulfate. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes Simplex Virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811PAPSBy similarity
Binding sitei189 – 1891PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1045PAPSBy similarity
Nucleotide bindingi305 – 3095PAPSBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 6 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 6
Short name:
3-OST-6
Short name:
Heparan sulfate 3-O-sulfotransferase 6
Short name:
h3-OST-6
Gene namesi
Name:HS3ST6
Synonyms:HS3ST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14178. HS3ST6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 4918Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini50 – 342293LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Polymorphism and mutation databases

DMDMi61214397.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Heparan sulfate glucosamine 3-O-sulfotransferase 6PRO_0000085224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi288 ↔ 300By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96QI5.
PRIDEiQ96QI5.

PTM databases

iPTMnetiQ96QI5.
PhosphoSiteiQ96QI5.

Expressioni

Gene expression databases

BgeeiENSG00000162040.
CleanExiHS_HS3ST5.
HS_HS3ST6.
ExpressionAtlasiQ96QI5. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000390354.

Structurei

3D structure databases

ProteinModelPortaliQ96QI5.
SMRiQ96QI5. Positions 74-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1287Substrate bindingBy similarity
Regioni153 – 1564Substrate bindingBy similarity
Regioni220 – 2212Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ96QI5.
KOiK09679.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ96QI5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96QI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSGGLGGG AGGGQGAGAG QGAALRASRA PMLLVALVLG AYCLCALPGR
60 70 80 90 100
CPPAARAPAP APAPSEPSSS VHRPGAPGLP LASGPGRRRF PQALIVGVKK
110 120 130 140 150
GGTRALLEFL RLHPDVRALG SEPHFFDRCY ERGLAWYRSL MPRTLDGQIT
160 170 180 190 200
MEKTPSYFVT REAPRRIHAM SPDTKLIVVV RNPVTRAISD YAQTLSKTPG
210 220 230 240 250
LPSFRALAFR HGLGPVDTAW SAVRIGLYAQ HLDHWLRYFP LSHFLFVSGE
260 270 280 290 300
RLVSDPAGEV GRVQDFLGLK RVVTDKHFYF NATKGFPCLK KAQGGSRPRC
310 320 330 340
LGKSKGRPHP RVPQALVRRL QEFYRPFNRR FYQMTGQDFG WG
Length:342
Mass (Da):37,186
Last modified:March 15, 2005 - v2
Checksum:i3CB8FFD4AFA7F9DC
GO

Sequence cautioni

The sequence AAK61299 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAC42157 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921A → T (PubMed:11157797).Curated
Sequence conflicti316 – 3161L → V (PubMed:11157797).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006640 Genomic DNA. Translation: AAK61299.1. Different initiation.
AL031723 Genomic DNA. Translation: CAC42157.1. Different initiation.
RefSeqiNP_001009606.3. NM_001009606.3.
UniGeneiHs.670144.

Genome annotation databases

EnsembliENST00000293937; ENSP00000293937; ENSG00000276333.
ENST00000454677; ENSP00000416741; ENSG00000162040.
GeneIDi64711.
KEGGihsa:64711.
UCSCiuc032epu.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006640 Genomic DNA. Translation: AAK61299.1. Different initiation.
AL031723 Genomic DNA. Translation: CAC42157.1. Different initiation.
RefSeqiNP_001009606.3. NM_001009606.3.
UniGeneiHs.670144.

3D structure databases

ProteinModelPortaliQ96QI5.
SMRiQ96QI5. Positions 74-341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000390354.

PTM databases

iPTMnetiQ96QI5.
PhosphoSiteiQ96QI5.

Polymorphism and mutation databases

DMDMi61214397.

Proteomic databases

PaxDbiQ96QI5.
PRIDEiQ96QI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293937; ENSP00000293937; ENSG00000276333.
ENST00000454677; ENSP00000416741; ENSG00000162040.
GeneIDi64711.
KEGGihsa:64711.
UCSCiuc032epu.2. human.

Organism-specific databases

CTDi64711.
GeneCardsiHS3ST6.
HGNCiHGNC:14178. HS3ST6.
neXtProtiNX_Q96QI5.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ96QI5.
KOiK09679.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ96QI5.

Enzyme and pathway databases

ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Miscellaneous databases

GenomeRNAii64711.
PROiQ96QI5.

Gene expression databases

BgeeiENSG00000162040.
CleanExiHS_HS3ST5.
HS_HS3ST6.
ExpressionAtlasiQ96QI5. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHS3S6_HUMAN
AccessioniPrimary (citable) accession number: Q96QI5
Secondary accession number(s): Q96RX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.