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Protein

PML-RARA-regulated adapter molecule 1

Gene

PRAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns4P.

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PML-RARA-regulated adapter molecule 1
Short name:
PRAM
Short name:
PRAM-1
Gene namesi
Name:PRAM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

EuPathDBiHostDB:ENSG00000133246.11
HGNCiHGNC:30091 PRAM1
MIMi606466 gene
neXtProtiNX_Q96QH2

Subcellular locationi

Pathology & Biotechi

Organism-specific databases

DisGeNETi84106
OpenTargetsiENSG00000133246
PharmGKBiPA142671137

Polymorphism and mutation databases

BioMutaiPRAM1
DMDMi327478532

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002701711 – 670PML-RARA-regulated adapter molecule 1Add BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei340PhosphoserineBy similarity1

Post-translational modificationi

May be phosphorylated on tyrosines.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96QH2
PeptideAtlasiQ96QH2
PRIDEiQ96QH2

PTM databases

iPTMnetiQ96QH2
PhosphoSitePlusiQ96QH2

Expressioni

Tissue specificityi

Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.1 Publication

Inductioni

Down-regulated by the PML-RARA oncogene, a fusion protein expressed in a vast majority of acute promyelocytic leukemia. Up-regulated by retinoic acid or arsenic trioxide in cells expressing PML-RARA.2 Publications

Gene expression databases

BgeeiENSG00000133246
CleanExiHS_PRAM1
ExpressionAtlasiQ96QH2 baseline and differential
GenevisibleiQ96QH2 HS

Organism-specific databases

HPAiHPA045236
HPA050161

Interactioni

Subunit structurei

Interacts with SKAP2, LCP2 and DBNL. May interact with LYN. Interacts with NEK6.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123894, 29 interactors
IntActiQ96QH2, 19 interactors

Structurei

3D structure databases

ProteinModelPortaliQ96QH2
SMRiQ96QH2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati70 – 811Add BLAST12
Repeati82 – 932Add BLAST12
Repeati94 – 1053Add BLAST12
Repeati106 – 1174Add BLAST12
Domaini571 – 649SH3PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 1654 X 12 AA repeats of K-P-P-[PQ]-P-[EQ]-[VAF]-T-D-L-P-KAdd BLAST96

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 463Pro-richPROSITE-ProRule annotationAdd BLAST437

Domaini

The SH3 domain binds to PtdIns4P.

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00530000063460
HOGENOMiHOG000168515
HOVERGENiHBG082165
InParanoidiQ96QH2
KOiK19994
OMAiFQASQPE
OrthoDBiEOG091G10IJ
PhylomeDBiQ96QH2

Family and domain databases

InterProiView protein in InterPro
IPR029294 hSH3
IPR036028 SH3-like_dom_sf
PfamiView protein in Pfam
PF14603 hSH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q96QH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHHLPAAME SHQDFRSIKA KFQASQPEPS DLPKKPPKPE FGKLKKFSQP
60 70 80 90 100
ELSEHPKKAP LPEFGAVSLK PPPPEVTDLP KKPPPPEVTD LPKKPPPPEV
110 120 130 140 150
TDLPKKPPPP EVTDLPKKPS KLELSDLSKK FPQLGATPFP RKPLQPEVGE
160 170 180 190 200
APLKASLPEP GAPARKPLQP DELSHPARPP SEPKSGAFPR KLWQPEAGEA
210 220 230 240 250
TPRSPQPELS TFPKKPAQPE FNVYPKKPPQ PQVGGLPKKS VPQPEFSEAA
260 270 280 290 300
QTPLWKPQSS EPKRDSSAFP KKASQPPLSD FPKKPPQPEL GDLTRTSSEP
310 320 330 340 350
EVSVLPKRPR PAEFKALSKK PPQPELGGLP RTSSEPEFNS LPRKLLQPER
360 370 380 390 400
RGPPRKFSQP EPSAVLKRHP QPEFFGDLPR KPPLPSSASE SSLPAAVAGF
410 420 430 440 450
SSRHPLSPGF GAAGTPRWRS GGLVHSGGAR PGLRPSHPPR RRPLPPASSL
460 470 480 490 500
GHPPAKPPLP PGPVDMQSFR RPSAASIDLR RTRSAAGLHF QDRQPEDIPQ
510 520 530 540 550
VPDEIYELYD DVEPRDDSSP SPKGRDEAPS VQQAARRPPQ DPALRKEKDP
560 570 580 590 600
QPQQLPPMDP KLLKQLRKAE KAEREFRKKF KFEGEIVVHT KMMIDPNAKT
610 620 630 640 650
RRGGGKHLGI RRGEILEVIE FTSNEEMLCR DPKGKYGYVP RTALLPLETE
660 670
VYDDVDFCDP LENQPLPLGR
Length:670
Mass (Da):73,969
Last modified:March 28, 2018 - v3
Checksum:i6C05FFBC05CE739F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11S → N in AAH28012 (PubMed:15489334).Curated1
Sequence conflicti72P → PQPQFTDLPKKPPPPEVTDL PKKPPPPEVTDLPKKPPPPE VTDLPKKPP in CAC17767 (PubMed:11301322).Curated1
Sequence conflicti76V → A in CAC17767 (PubMed:11301322).Curated1

Polymorphismi

Some transcripts displayed additional 12 amino acid repeats of K-P-P-[PQ]-P-[EQ]-[VAF]-T-D-L-P-K (PubMed:11301322). We cannot rule out that they may represent genetic variants.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02980857K → Q1 PublicationCorresponds to variant dbSNP:rs4804305Ensembl.1
Natural variantiVAR_02980973P → Q. Corresponds to variant dbSNP:rs4239541Ensembl.1
Natural variantiVAR_02981076V → F. Corresponds to variant dbSNP:rs4239540Ensembl.1
Natural variantiVAR_061692135G → E. Corresponds to variant dbSNP:rs58466313Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272324 mRNA Translation: CAC17767.1
AC092298 Genomic DNA No translation available.
AC136469 Genomic DNA No translation available.
BC028012 mRNA Translation: AAH28012.1
UniGeneiHs.465812

Genome annotation databases

EnsembliENST00000423345; ENSP00000408342; ENSG00000133246
GeneIDi84106
KEGGihsa:84106
UCSCiuc002mkd.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPRAM_HUMAN
AccessioniPrimary (citable) accession number: Q96QH2
Secondary accession number(s): Q8N6W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 28, 2018
Last modified: April 25, 2018
This is version 120 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health