Q96QH2 (PRAM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PML-RARA-regulated adapter molecule 1
Short name=PRAM
Short name=PRAM-1

Gene names

Name:

PRAM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns4P.
Subunit structure	Interacts with SKAP2, LCP2 and DBNL. May interact with LYN. Interacts with NEK6. Ref.1 Ref.4 Ref.7
Tissue specificity	Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung. Ref.1
Induction	Down-regulated by the PML-RARA oncogene, a fusion protein expressed in a vast majority of acute promyelocytic leukemia. Up-regulated by retinoic acid or arsenic trioxide in cells expressing PML-RARA. Ref.1 Ref.4
Domain	The SH3 domain binds to PtdIns4P.
Post-translational modification	May be phosphorylated on tyrosines. Ref.1
Sequence similarities	Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat SH3 domain
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	integrin-mediated signaling pathway Inferred from electronic annotation. Source: Compara regulation of neutrophil degranulation Inferred from electronic annotation. Source: Compara
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNF32	Q9H0A6	2	EBI-2860740,EBI-724829

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96QH2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96QH2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-117: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	PML-RARA-regulated adapter molecule 1		PRO_0000270171
Regions
	Repeat	70 – 81	12	1
	Repeat	82 – 93	12	2
	Repeat	94 – 105	12	3
	Repeat	106 – 117	12	4
	Repeat	118 – 129	12	5
	Repeat	130 – 141	12	6
	Repeat	142 – 153	12	7
	Repeat	154 – 165	12	8
	Domain	622 – 696	75	SH3
	Region	70 – 165	96	8 X 12 AA repeats of K-P-P-[PQ]-P-[EQ]-[VAF]-T-D-L-P-K
	Compositional bias	27 – 511	485	Pro-rich
Amino acid modifications
	Modified residue	382	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	70 – 117	48	Missing in isoform 2.		VSP_022178
	Natural variant	57	1	K → Q. Ref.1 Corresponds to variant rs4804305 [ dbSNP | Ensembl ].		VAR_029808
	Natural variant	73	1	Q → P. Corresponds to variant rs4239541 [ dbSNP | Ensembl ].		VAR_029809
	Natural variant	76	1	F → V. Corresponds to variant rs4239540 [ dbSNP | Ensembl ].		VAR_029810
	Natural variant	183	1	G → E. Corresponds to variant rs58466313 [ dbSNP | Ensembl ].		VAR_061692
Experimental info
	Sequence conflict	11	1	S → N in AAH28012. Ref.3
	Sequence conflict	124	1	V → A in CAC17767. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 5, 2011. Version 2. Checksum: 212A6BA694578F2D	FASTA	718	79,245
	10 20 30 40 50 60 MAHHLPAAME SHQDFRSIKA KFQASQPEPS DLPKKPPKPE FGKLKKFSQP ELSEHPKKAP 70 80 90 100 110 120 LPEFGAVSLK PPQPQFTDLP KKPPPPEVTD LPKKPPPPEV TDLPKKPPPP EVTDLPKKPP 130 140 150 160 170 180 PPEVTDLPKK PPPPEVTDLP KKPPPPEVTD LPKKPPPPEV TDLPKKPSKL ELSDLSKKFP 190 200 210 220 230 240 QLGATPFPRK PLQPEVGEAP LKASLPEPGA PARKPLQPDE LSHPARPPSE PKSGAFPRKL 250 260 270 280 290 300 WQPEAGEATP RSPQPELSTF PKKPAQPEFN VYPKKPPQPQ VGGLPKKSVP QPEFSEAAQT 310 320 330 340 350 360 PLWKPQSSEP KRDSSAFPKK ASQPPLSDFP KKPPQPELGD LTRTSSEPEV SVLPKRPRPA 370 380 390 400 410 420 EFKALSKKPP QPELGGLPRT SSEPEFNSLP RKLLQPERRG PPRKFSQPEP SAVLKRHPQP 430 440 450 460 470 480 EFFGDLPRKP PLPSSASESS LPAAVAGFSS RHPLSPGFGA AGTPRWRSGG LVHSGGARPG 490 500 510 520 530 540 LRPSHPPRRR PLPPASSLGH PPAKPPLPPG PVDMQSFRRP SAASIDLRRT RSAAGLHFQD 550 560 570 580 590 600 RQPEDIPQVP DEIYELYDDV EPRDDSSPSP KGRDEAPSVQ QAARRPPQDP ALRKEKDPQP 610 620 630 640 650 660 QQLPPMDPKL LKQLRKAEKA EREFRKKFKF EGEIVVHTKM MIDPNAKTRR GGGKHLGIRR 670 680 690 700 710 GEILEVIEFT SNEEMLCRDP KGKYGYVPRT ALLPLETEVY DDVDFCDPLE NQPLPLGR
	Isoform 2 [UniParc]. Checksum: 6C05FFBC05CE739F	FASTA	670	73,969
	10 20 30 40 50 60 MAHHLPAAME SHQDFRSIKA KFQASQPEPS DLPKKPPKPE FGKLKKFSQP ELSEHPKKAP 70 80 90 100 110 120 LPEFGAVSLK PPPPEVTDLP KKPPPPEVTD LPKKPPPPEV TDLPKKPPPP EVTDLPKKPS 130 140 150 160 170 180 KLELSDLSKK FPQLGATPFP RKPLQPEVGE APLKASLPEP GAPARKPLQP DELSHPARPP 190 200 210 220 230 240 SEPKSGAFPR KLWQPEAGEA TPRSPQPELS TFPKKPAQPE FNVYPKKPPQ PQVGGLPKKS 250 260 270 280 290 300 VPQPEFSEAA QTPLWKPQSS EPKRDSSAFP KKASQPPLSD FPKKPPQPEL GDLTRTSSEP 310 320 330 340 350 360 EVSVLPKRPR PAEFKALSKK PPQPELGGLP RTSSEPEFNS LPRKLLQPER RGPPRKFSQP 370 380 390 400 410 420 EPSAVLKRHP QPEFFGDLPR KPPLPSSASE SSLPAAVAGF SSRHPLSPGF GAAGTPRWRS 430 440 450 460 470 480 GGLVHSGGAR PGLRPSHPPR RRPLPPASSL GHPPAKPPLP PGPVDMQSFR RPSAASIDLR 490 500 510 520 530 540 RTRSAAGLHF QDRQPEDIPQ VPDEIYELYD DVEPRDDSSP SPKGRDEAPS VQQAARRPPQ 550 560 570 580 590 600 DPALRKEKDP QPQQLPPMDP KLLKQLRKAE KAEREFRKKF KFEGEIVVHT KMMIDPNAKT 610 620 630 640 650 660 RRGGGKHLGI RRGEILEVIE FTSNEEMLCR DPKGKYGYVP RTALLPLETE VYDDVDFCDP 670 LENQPLPLGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells." Moog-Lutz C., Peterson E.J., Lutz P.G., Eliason S., Cave-Riant F., Singer A., Di Gioia Y., Dmovski S., Kamens J., Cayre Y.E., Koretzky G. J. Biol. Chem. 276:22375-22381(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH SKAP2; LCP2 AND LYN, VARIANT GLN-57.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Blood.
[4]	"PRAM-1 potentiates arsenic trioxide-induced JNK activation." Denis F.M., Benecke A., Di Gioia Y., Touw I.P., Cayre Y.E., Lutz P.G. J. Biol. Chem. 280:9043-9048(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DBNL, INDUCTION.
[5]	"Lipid-binding hSH3 domains in immune cell adapter proteins." Heuer K., Sylvester M., Kliche S., Pusch R., Thiemke K., Schraven B., Freund C. J. Mol. Biol. 361:94-104(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHOINOSITIDE-BINDING.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome." Vaz Meirelles G., Ferreira Lanza D.C., da Silva J.C., Santana Bernachi J., Paes Leme A.F., Kobarg J. J. Proteome Res. 9:6298-6316(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NEK6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ272324 mRNA. Translation: CAC17767.1. AC092298 Genomic DNA. No translation available. AC136469 Genomic DNA. No translation available. BC028012 mRNA. Translation: AAH28012.1.
IPI	IPI00335147. IPI00816773.
RefSeq	NP_115528.4. NM_032152.4.
UniGene	Hs.465812.
3D structure databases
HSSP	HSSP built from PDB template 1RI9 based on UniProtKB O15117.
ProteinModelPortal	Q96QH2.
SMR	Q96QH2. Positions 618-692.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96QH2. 7 interactions.
STRING	9606.ENSP00000255612.
PTM databases
PhosphoSite	Q96QH2.
Polymorphism databases
DMDM	74717183.
Proteomic databases
PaxDb	Q96QH2.
PRIDE	Q96QH2.
Protocols and materials databases
DNASU	84106.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000423345; ENSP00000408342; ENSG00000133246.
GeneID	84106.
KEGG	hsa:84106.
UCSC	uc002mkd.3. human.
Organism-specific databases
CTD	84106.
GeneCards	GC19M008554.
H-InvDB	HIX0202846.
HGNC	HGNC:30091. PRAM1.
HPA	HPA050161.
MIM	606466. gene.
neXtProt	NX_Q96QH2.
PharmGKB	PA142671137.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000168515.
HOVERGEN	HBG082165.
InParanoid	Q96QH2.
OMA	FQASQPE.
OrthoDB	EOG4F1X2R.
Gene expression databases
Bgee	Q96QH2.
CleanEx	HS_PRAM1.
Genevestigator	Q96QH2.
Family and domain databases
InterPro	IPR001452. SH3_domain. [Graphical view]
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84106.
NextBio	73367.
SOURCE	Search...

Entry information

Entry name

PRAM_HUMAN

Accession

Primary (citable) accession number: Q96QH2
Secondary accession number(s): Q8N6W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	April 5, 2011
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.