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Q96QH2

- PRAM_HUMAN

UniProt

Q96QH2 - PRAM_HUMAN

Protein

PML-RARA-regulated adapter molecule 1

Gene

PRAM1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns4P.

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. integrin-mediated signaling pathway Source: Ensembl
    2. regulation of neutrophil degranulation Source: Ensembl

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PML-RARA-regulated adapter molecule 1
    Short name:
    PRAM
    Short name:
    PRAM-1
    Gene namesi
    Name:PRAM1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:30091. PRAM1.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671137.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 718718PML-RARA-regulated adapter molecule 1PRO_0000270171Add
    BLAST

    Post-translational modificationi

    May be phosphorylated on tyrosines.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ96QH2.
    PRIDEiQ96QH2.

    PTM databases

    PhosphoSiteiQ96QH2.

    Expressioni

    Tissue specificityi

    Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.1 Publication

    Inductioni

    Down-regulated by the PML-RARA oncogene, a fusion protein expressed in a vast majority of acute promyelocytic leukemia. Up-regulated by retinoic acid or arsenic trioxide in cells expressing PML-RARA.2 Publications

    Gene expression databases

    ArrayExpressiQ96QH2.
    BgeeiQ96QH2.
    CleanExiHS_PRAM1.
    GenevestigatoriQ96QH2.

    Organism-specific databases

    HPAiHPA050161.

    Interactioni

    Subunit structurei

    Interacts with SKAP2, LCP2 and DBNL. May interact with LYN. Interacts with NEK6.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RNF32Q9H0A62EBI-2860740,EBI-724829

    Protein-protein interaction databases

    BioGridi123894. 20 interactions.
    IntActiQ96QH2. 7 interactions.
    STRINGi9606.ENSP00000255612.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96QH2.
    SMRiQ96QH2. Positions 618-692.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati70 – 81121Add
    BLAST
    Repeati82 – 93122Add
    BLAST
    Repeati94 – 105123Add
    BLAST
    Repeati106 – 117124Add
    BLAST
    Repeati118 – 129125Add
    BLAST
    Repeati130 – 141126Add
    BLAST
    Repeati142 – 153127Add
    BLAST
    Repeati154 – 165128Add
    BLAST
    Domaini622 – 69675SH3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni70 – 165968 X 12 AA repeats of K-P-P-[PQ]-P-[EQ]-[VAF]-T-D-L-P-KAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi27 – 511485Pro-richAdd
    BLAST

    Domaini

    The SH3 domain binds to PtdIns4P.

    Sequence similaritiesi

    Contains 1 SH3 domain.Curated

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000168515.
    HOVERGENiHBG082165.
    InParanoidiQ96QH2.
    OMAiFQASQPE.
    OrthoDBiEOG75QR3T.
    PhylomeDBiQ96QH2.

    Family and domain databases

    InterProiIPR029294. hSH3.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF14603. hSH3. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96QH2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAHHLPAAME SHQDFRSIKA KFQASQPEPS DLPKKPPKPE FGKLKKFSQP    50
    ELSEHPKKAP LPEFGAVSLK PPQPQFTDLP KKPPPPEVTD LPKKPPPPEV 100
    TDLPKKPPPP EVTDLPKKPP PPEVTDLPKK PPPPEVTDLP KKPPPPEVTD 150
    LPKKPPPPEV TDLPKKPSKL ELSDLSKKFP QLGATPFPRK PLQPEVGEAP 200
    LKASLPEPGA PARKPLQPDE LSHPARPPSE PKSGAFPRKL WQPEAGEATP 250
    RSPQPELSTF PKKPAQPEFN VYPKKPPQPQ VGGLPKKSVP QPEFSEAAQT 300
    PLWKPQSSEP KRDSSAFPKK ASQPPLSDFP KKPPQPELGD LTRTSSEPEV 350
    SVLPKRPRPA EFKALSKKPP QPELGGLPRT SSEPEFNSLP RKLLQPERRG 400
    PPRKFSQPEP SAVLKRHPQP EFFGDLPRKP PLPSSASESS LPAAVAGFSS 450
    RHPLSPGFGA AGTPRWRSGG LVHSGGARPG LRPSHPPRRR PLPPASSLGH 500
    PPAKPPLPPG PVDMQSFRRP SAASIDLRRT RSAAGLHFQD RQPEDIPQVP 550
    DEIYELYDDV EPRDDSSPSP KGRDEAPSVQ QAARRPPQDP ALRKEKDPQP 600
    QQLPPMDPKL LKQLRKAEKA EREFRKKFKF EGEIVVHTKM MIDPNAKTRR 650
    GGGKHLGIRR GEILEVIEFT SNEEMLCRDP KGKYGYVPRT ALLPLETEVY 700
    DDVDFCDPLE NQPLPLGR 718
    Length:718
    Mass (Da):79,245
    Last modified:April 5, 2011 - v2
    Checksum:i212A6BA694578F2D
    GO
    Isoform 2 (identifier: Q96QH2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         70-117: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:670
    Mass (Da):73,969
    Checksum:i6C05FFBC05CE739F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111S → N in AAH28012. (PubMed:15489334)Curated
    Sequence conflicti124 – 1241V → A in CAC17767. (PubMed:11301322)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti57 – 571K → Q.1 Publication
    Corresponds to variant rs4804305 [ dbSNP | Ensembl ].
    VAR_029808
    Natural varianti73 – 731Q → P.
    Corresponds to variant rs4239541 [ dbSNP | Ensembl ].
    VAR_029809
    Natural varianti76 – 761F → V.
    Corresponds to variant rs4239540 [ dbSNP | Ensembl ].
    VAR_029810
    Natural varianti183 – 1831G → E.
    Corresponds to variant rs58466313 [ dbSNP | Ensembl ].
    VAR_061692

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei70 – 11748Missing in isoform 2. 1 PublicationVSP_022178Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ272324 mRNA. Translation: CAC17767.1.
    AC092298 Genomic DNA. No translation available.
    AC136469 Genomic DNA. No translation available.
    BC028012 mRNA. Translation: AAH28012.1.
    CCDSiCCDS45954.2. [Q96QH2-2]
    RefSeqiNP_115528.4. NM_032152.4. [Q96QH2-2]
    UniGeneiHs.465812.

    Genome annotation databases

    GeneIDi84106.
    KEGGihsa:84106.
    UCSCiuc002mkd.3. human. [Q96QH2-2]

    Polymorphism databases

    DMDMi327478532.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ272324 mRNA. Translation: CAC17767.1 .
    AC092298 Genomic DNA. No translation available.
    AC136469 Genomic DNA. No translation available.
    BC028012 mRNA. Translation: AAH28012.1 .
    CCDSi CCDS45954.2. [Q96QH2-2 ]
    RefSeqi NP_115528.4. NM_032152.4. [Q96QH2-2 ]
    UniGenei Hs.465812.

    3D structure databases

    ProteinModelPortali Q96QH2.
    SMRi Q96QH2. Positions 618-692.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123894. 20 interactions.
    IntActi Q96QH2. 7 interactions.
    STRINGi 9606.ENSP00000255612.

    PTM databases

    PhosphoSitei Q96QH2.

    Polymorphism databases

    DMDMi 327478532.

    Proteomic databases

    PaxDbi Q96QH2.
    PRIDEi Q96QH2.

    Protocols and materials databases

    DNASUi 84106.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 84106.
    KEGGi hsa:84106.
    UCSCi uc002mkd.3. human. [Q96QH2-2 ]

    Organism-specific databases

    CTDi 84106.
    GeneCardsi GC19M008554.
    H-InvDB HIX0202846.
    HGNCi HGNC:30091. PRAM1.
    HPAi HPA050161.
    MIMi 606466. gene.
    neXtProti NX_Q96QH2.
    PharmGKBi PA142671137.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000168515.
    HOVERGENi HBG082165.
    InParanoidi Q96QH2.
    OMAi FQASQPE.
    OrthoDBi EOG75QR3T.
    PhylomeDBi Q96QH2.

    Miscellaneous databases

    GeneWikii PRAM1.
    GenomeRNAii 84106.
    NextBioi 73367.
    PROi Q96QH2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96QH2.
    Bgeei Q96QH2.
    CleanExi HS_PRAM1.
    Genevestigatori Q96QH2.

    Family and domain databases

    InterProi IPR029294. hSH3.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF14603. hSH3. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells."
      Moog-Lutz C., Peterson E.J., Lutz P.G., Eliason S., Cave-Riant F., Singer A., Di Gioia Y., Dmovski S., Kamens J., Cayre Y.E., Koretzky G.
      J. Biol. Chem. 276:22375-22381(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH SKAP2; LCP2 AND LYN, VARIANT GLN-57.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Blood.
    4. "PRAM-1 potentiates arsenic trioxide-induced JNK activation."
      Denis F.M., Benecke A., Di Gioia Y., Touw I.P., Cayre Y.E., Lutz P.G.
      J. Biol. Chem. 280:9043-9048(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DBNL, INDUCTION.
    5. "Lipid-binding hSH3 domains in immune cell adapter proteins."
      Heuer K., Sylvester M., Kliche S., Pusch R., Thiemke K., Schraven B., Freund C.
      J. Mol. Biol. 361:94-104(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHOINOSITIDE-BINDING.
    6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome."
      Vaz Meirelles G., Ferreira Lanza D.C., da Silva J.C., Santana Bernachi J., Paes Leme A.F., Kobarg J.
      J. Proteome Res. 9:6298-6316(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEK6.

    Entry informationi

    Entry nameiPRAM_HUMAN
    AccessioniPrimary (citable) accession number: Q96QH2
    Secondary accession number(s): Q8N6W7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3