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Protein

Sodium-coupled neutral amino acid transporter 2

Gene

SLC38A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. May function in the transport of amino acids at the blood-brain barrier and in the supply of maternal nutrients to the fetus through the placenta.2 Publications

Miscellaneous

Depletion of SCL38A2 by siRNA prevents the recovery of cells from hypertonic stress.

Enzyme regulationi

Inhibited by N-methyl-D-glucamine and probably choline.1 Publication

Kineticsi

  1. KM=0.39 mM for 2-methylamino-isobutyric acid (MeAIB) (at pH 8.0)

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Biological processAmino-acid transport, Ion transport, Sodium transport, Symport, Transport
    LigandSodium

    Enzyme and pathway databases

    ReactomeiR-HSA-210500 Glutamate Neurotransmitter Release Cycle
    R-HSA-352230 Amino acid transport across the plasma membrane

    Protein family/group databases

    TCDBi2.A.18.6.5 the amino acid/auxin permease (aaap) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 2
    Alternative name(s):
    Amino acid transporter A2
    Protein 40-9-1
    Solute carrier family 38 member 2
    System A amino acid transporter 2
    System A transporter 1
    System N amino acid transporter 2
    Gene namesi
    Name:SLC38A2
    Synonyms:ATA2, KIAA1382, SAT2, SNAT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 12

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000134294.13
    HGNCiHGNC:13448 SLC38A2
    MIMi605180 gene
    neXtProtiNX_Q96QD8

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 76CytoplasmicSequence analysisAdd BLAST76
    Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
    Topological domaini98 – 102ExtracellularSequence analysis5
    Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
    Topological domaini124 – 158CytoplasmicSequence analysisAdd BLAST35
    Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
    Topological domaini180 – 188ExtracellularSequence analysis9
    Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
    Topological domaini210 – 217CytoplasmicSequence analysis8
    Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
    Topological domaini239 – 292ExtracellularSequence analysisAdd BLAST54
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 329CytoplasmicSequence analysisAdd BLAST16
    Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
    Topological domaini351 – 371ExtracellularSequence analysisAdd BLAST21
    Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
    Topological domaini393 – 413CytoplasmicSequence analysisAdd BLAST21
    Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
    Topological domaini435 – 436ExtracellularSequence analysis2
    Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
    Topological domaini458 – 474CytoplasmicSequence analysisAdd BLAST17
    Transmembranei475 – 495HelicalSequence analysisAdd BLAST21
    Topological domaini496 – 506ExtracellularSequence analysisAdd BLAST11

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi54407
    OpenTargetsiENSG00000134294
    PharmGKBiPA37773

    Polymorphism and mutation databases

    BioMutai2/14/2002
    DMDMi162416227

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003113691 – 506Sodium-coupled neutral amino acid transporter 2Add BLAST506

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei10PhosphoserineCombined sources1
    Modified residuei12PhosphoserineCombined sources1
    Modified residuei21PhosphoserineBy similarity1
    Modified residuei22PhosphoserineCombined sources1
    Modified residuei55PhosphoserineCombined sources1
    Disulfide bondi245 ↔ 281PROSITE-ProRule annotation
    Glycosylationi258N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    Polyubiquitination by NEDD4L regulates the degradation and the activity of SLC38A2.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    EPDiQ96QD8
    MaxQBiQ96QD8
    PaxDbiQ96QD8
    PeptideAtlasiQ96QD8
    PRIDEiQ96QD8

    PTM databases

    iPTMnetiQ96QD8
    PhosphoSitePlusiQ96QD8
    SwissPalmiQ96QD8

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Widely expressed in the central nervous system with higher concentrations in caudal regions. Expressed by glutamatergic and GABAergic neurons together with astrocytes and other non-neuronal cells in the cerebral cortex (at protein level).3 Publications

    Inductioni

    Up-regulated upon hypertonic conditions and amino acid deprivation.3 Publications

    Gene expression databases

    BgeeiENSG00000134294
    CleanExiHS_SAT2
    HS_SLC38A2
    ExpressionAtlasiQ96QD8 baseline and differential
    GenevisibleiQ96QD8 HS

    Organism-specific databases

    HPAiHPA035180

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RNF5Q999423EBI-723083,EBI-348482

    Protein-protein interaction databases

    BioGridi119943, 29 interactors
    IntActiQ96QD8, 28 interactors
    MINTiQ96QD8
    STRINGi9606.ENSP00000256689

    Structurei

    3D structure databases

    ProteinModelPortaliQ96QD8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 96Regulates protein turnover upon amino acid deprivationBy similarityAdd BLAST96

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1305 Eukaryota
    COG0814 LUCA
    GeneTreeiENSGT00760000119147
    HOGENOMiHOG000013088
    HOVERGENiHBG059571
    InParanoidiQ96QD8
    KOiK14207
    OMAiALMNIED
    OrthoDBiEOG091G0GP8
    PhylomeDBiQ96QD8
    TreeFamiTF328787

    Family and domain databases

    InterProiView protein in InterPro
    IPR013057 AA_transpt_TM
    PfamiView protein in Pfam
    PF01490 Aa_trans, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q96QD8-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKKAEMGRFS ISPDEDSSSY SSNSDFNYSY PTKQAALKSH YADVDPENQN
    60 70 80 90 100
    FLLESNLGKK KYETEFHPGT TSFGMSVFNL SNAIVGSGIL GLSYAMANTG
    110 120 130 140 150
    IALFIILLTF VSIFSLYSVH LLLKTANEGG SLLYEQLGYK AFGLVGKLAA
    160 170 180 190 200
    SGSITMQNIG AMSSYLFIVK YELPLVIQAL TNIEDKTGLW YLNGNYLVLL
    210 220 230 240 250
    VSLVVILPLS LFRNLGYLGY TSGLSLLCMV FFLIVVICKK FQVPCPVEAA
    260 270 280 290 300
    LIINETINTT LTQPTALVPA LSHNVTENDS CRPHYFIFNS QTVYAVPILI
    310 320 330 340 350
    FSFVCHPAVL PIYEELKDRS RRRMMNVSKI SFFAMFLMYL LAALFGYLTF
    360 370 380 390 400
    YEHVESELLH TYSSILGTDI LLLIVRLAVL MAVTLTVPVV IFPIRSSVTH
    410 420 430 440 450
    LLCASKDFSW WRHSLITVSI LAFTNLLVIF VPTIRDIFGF IGASAASMLI
    460 470 480 490 500
    FILPSAFYIK LVKKEPMKSV QKIGALFFLL SGVLVMTGSM ALIVLDWVHN

    APGGGH
    Length:506
    Mass (Da):56,026
    Last modified:December 4, 2007 - v2
    Checksum:i125C70875793D031
    GO
    Isoform 2 (identifier: Q96QD8-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-100: Missing.
         101-105: IALFI → MKQNL

    Show »
    Length:406
    Mass (Da):45,179
    Checksum:iC72772C3238776CE
    GO

    Sequence cautioni

    The sequence BAD18765 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti156M → V in CAC51434 (Ref. 3) Curated1
    Sequence conflicti273H → R in BAA91846 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_03723548N → K. Corresponds to variant dbSNP:rs11183450Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0295531 – 100Missing in isoform 2. 2 PublicationsAdd BLAST100
    Alternative sequenceiVSP_029554101 – 105IALFI → MKQNL in isoform 2. 2 Publications5

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF259799 mRNA Translation: AAK38510.1
    AF298897 mRNA Translation: AAG24618.1
    AJ344099 mRNA Translation: CAC51434.1
    AB037803 mRNA Translation: BAA92620.2
    AK001700 mRNA Translation: BAA91846.1
    AK172784 mRNA Translation: BAD18765.1 Different initiation.
    CR457267 mRNA Translation: CAG33548.1
    CH471111 Genomic DNA Translation: EAW57900.1
    CH471111 Genomic DNA Translation: EAW57901.1
    BC040342 mRNA Translation: AAH40342.1
    CCDSiCCDS76551.1 [Q96QD8-2]
    CCDS8749.1 [Q96QD8-1]
    RefSeqiNP_001294865.1, NM_001307936.1 [Q96QD8-2]
    NP_061849.2, NM_018976.4 [Q96QD8-1]
    UniGeneiHs.221847

    Genome annotation databases

    EnsembliENST00000256689; ENSP00000256689; ENSG00000134294 [Q96QD8-1]
    ENST00000612232; ENSP00000482873; ENSG00000134294 [Q96QD8-2]
    GeneIDi54407
    KEGGihsa:54407
    UCSCiuc001rpg.4 human [Q96QD8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiS38A2_HUMAN
    AccessioniPrimary (citable) accession number: Q96QD8
    Secondary accession number(s): Q6IA88
    , Q6ZMG2, Q9HAV3, Q9NVA8, Q9P2G5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: December 4, 2007
    Last modified: April 25, 2018
    This is version 136 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health