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Q96Q91 (B3A4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anion exchange protein 4

Short name=AE 4
Short name=Anion exchanger 4
Alternative name(s):
Sodium bicarbonate cotransporter 5
Solute carrier family 4 member 9
Gene names
Name:SLC4A9
Synonyms:AE4, SBC5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length983 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable apical anion exchanger of the kidney cortex By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Kidney specific. Ref.2 Ref.7

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Sequence caution

The sequence AAK28832.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96Q91-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96Q91-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-100: Missing.
     384-394: Missing.
     592-594: Missing.
Isoform 3 (identifier: Q96Q91-3)

The sequence of this isoform differs from the canonical sequence as follows:
     77-100: Missing.
Isoform 4 (identifier: Q96Q91-4)

The sequence of this isoform differs from the canonical sequence as follows:
     77-100: Missing.
     595-657: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 983983Anion exchange protein 4
PRO_0000079223

Regions

Topological domain1 – 414414Cytoplasmic Potential
Transmembrane415 – 43521Helical; Potential
Transmembrane443 – 46321Helical; Potential
Transmembrane465 – 48521Helical; Potential
Transmembrane500 – 52021Helical; Potential
Transmembrane530 – 55021Helical; Potential
Topological domain551 – 62373Extracellular Potential
Transmembrane624 – 64421Helical; Potential
Transmembrane665 – 68521Helical; Potential
Topological domain686 – 71126Cytoplasmic Potential
Transmembrane712 – 73221Helical; Potential
Transmembrane758 – 77821Helical; Potential
Transmembrane815 – 83521Helical; Potential
Transmembrane837 – 85721Helical; Potential
Transmembrane899 – 91921Helical; Potential
Region415 – 983569Membrane (anion exchange)

Amino acid modifications

Glycosylation5761N-linked (GlcNAc...) Potential
Glycosylation6001N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence77 – 10024Missing in isoform 2, isoform 3 and isoform 4.
VSP_007085
Alternative sequence384 – 39411Missing in isoform 2.
VSP_007086
Alternative sequence592 – 5943Missing in isoform 2.
VSP_007087
Alternative sequence595 – 65763Missing in isoform 4.
VSP_044785

Experimental info

Sequence conflict231G → W in AAI36263. Ref.5
Sequence conflict231G → W in AAI43603. Ref.5
Sequence conflict1011R → T in AAK69625. Ref.4
Sequence conflict4531V → M in AAK16733. Ref.1
Sequence conflict7951R → K in AAK16733. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 28, 2003. Version 2.
Checksum: E2AEB04C9D27E6BC

FASTA983108,248
        10         20         30         40         50         60 
MEMKLPGQEG FEASSAPRNI PSGELDSNPD PGTGPSPDGP SDTESKELGV PKDPLLFIQL 

        70         80         90        100        110        120 
NELLGWPQAL EWRETGSSSA SLLLDMGEMP SITLSTHLHH RWVLFEEKLE VAAGRWSAPH 

       130        140        150        160        170        180 
VPTLALPSLQ KLRSLLAEGL VLLDCPAQSL LELVEQVTRV ESLSPELRGQ LQALLLQRPQ 

       190        200        210        220        230        240 
HYNQTTGTRP CWGSTHPRKA SDNEEAPLRE QCQNPLRQKL PPGAEAGTVL AGELGFLAQP 

       250        260        270        280        290        300 
LGAFVRLRNP VVLGSLTEVS LPSRFFCLLL GPCMLGKGYH EMGRAAAVLL SDPQFQWSVR 

       310        320        330        340        350        360 
RASNLHDLLA ALDAFLEEVT VLPPGRWDPT ARIPPPKCLP SQHKRLPSQQ REIRGPAVPR 

       370        380        390        400        410        420 
LTSAEDRHRH GPHAHSPELQ RTGRLFGGLI QDVRRKVPWY PSDFLDALHL QCFSAVLYIY 

       430        440        450        460        470        480 
LATVTNAITF GGLLGDATDG AQGVLESFLG TAVAGAAFCL MAGQPLTILS STGPVLVFER 

       490        500        510        520        530        540 
LLFSFSRDYS LDYLPFRLWV GIWVATFCLV LVATEASVLV RYFTRFTEEG FCALISLIFI 

       550        560        570        580        590        600 
YDAVGKMLNL THTYPIQKPG SSAYGCLCQY PGPGGNESQW IRTRPKDRDD IVSMDLGLIN 

       610        620        630        640        650        660 
ASLLPPPECT RQGGHPRGPG CHTVPDIAFF SLLLFLTSFF FAMALKCVKT SRFFPSVVRK 

       670        680        690        700        710        720 
GLSDFSSVLA ILLGCGLDAF LGLATPKLMV PREFKPTLPG RGWLVSPFGA NPWWWSVAAA 

       730        740        750        760        770        780 
LPALLLSILI FMDQQITAVI LNRMEYRLQK GAGFHLDLFC VAVLMLLTSA LGLPWYVSAT 

       790        800        810        820        830        840 
VISLAHMDSL RRESRACAPG ERPNFLGIRE QRLTGLVVFI LTGASIFLAP VLKFIPMPVL 

       850        860        870        880        890        900 
YGIFLYMGVA ALSSIQFTNR VKLLLMPAKH QPDLLLLRHV PLTRVHLFTA IQLACLGLLW 

       910        920        930        940        950        960 
IIKSTPAAII FPLMLLGLVG VRKALERVFS PQELLWLDEL MPEEERSIPE KGLEPEHSFS 

       970        980 
GSDSEDSELM YQPKAPEINI SVN 

« Hide

Isoform 2 [UniParc].

Checksum: 4221B3EF5309C1C0
Show »

FASTA945104,128
Isoform 3 [UniParc].

Checksum: 2B190D6A5D2E346C
Show »

FASTA959105,701
Isoform 4 [UniParc].

Checksum: B6E908619855FCCC
Show »

FASTA89698,851

References

« Hide 'large scale' references
[1]"Human BTR1, a new bicarbonate transporter superfamily member and human AE4 from kidney."
Parker M.D., Ourmozdi E.P., Tanner M.J.A.
Biochem. Biophys. Res. Commun. 282:1103-1109(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Kidney and Testis.
[2]"A novel sodium bicarbonate cotransporter-like gene in an ancient duplicated region: SLC4A9 at 5q31."
Lipovich L., Lynch E.D., Lee M.K., King M.-C.
Genome Biol. 2:RESEARCH0011.1-RESEARCH0011.13(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Kidney.
[3]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Cloning and characterization of human AE4."
Karet F.E.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Lung, Placenta and Testis.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"Molecular cloning of human sodium bicarbonate cotransporter 5."
Ishibashi K.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-983 (ISOFORM 3), TISSUE SPECIFICITY.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF336237 mRNA. Translation: AAK16733.1.
AF313465 mRNA. Translation: AAK28832.1. Different initiation.
AF332961 mRNA. Translation: AAK69625.1.
AC008438 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62062.1.
CH471062 Genomic DNA. Translation: EAW62063.1.
CH471062 Genomic DNA. Translation: EAW62064.1.
CH471062 Genomic DNA. Translation: EAW62066.1.
CH471062 Genomic DNA. Translation: EAW62067.1.
CH471062 Genomic DNA. Translation: EAW62068.1.
BC136262 mRNA. Translation: AAI36263.1.
BC143602 mRNA. Translation: AAI43603.1.
AB032762 mRNA. Translation: BAA93010.1.
RefSeqNP_001245355.1. NM_001258426.1.
NP_001245356.1. NM_001258427.1.
NP_001245357.1. NM_001258428.1.
NP_113655.2. NM_031467.2.
XP_005268578.1. XM_005268521.1.
UniGeneHs.550313.

3D structure databases

ProteinModelPortalQ96Q91.
SMRQ96Q91. Positions 54-350, 396-437, 855-887.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000387708.

Protein family/group databases

TCDB2.A.31.2.13. the anion exchanger (ae) family.

PTM databases

PhosphoSiteQ96Q91.

Polymorphism databases

DMDM29427950.

Proteomic databases

PaxDbQ96Q91.
PRIDEQ96Q91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000230993; ENSP00000230993; ENSG00000113073. [Q96Q91-1]
ENST00000432095; ENSP00000410056; ENSG00000113073. [Q96Q91-2]
ENST00000506545; ENSP00000422855; ENSG00000113073. [Q96Q91-4]
ENST00000506757; ENSP00000424424; ENSG00000113073. [Q96Q91-3]
ENST00000507527; ENSP00000427661; ENSG00000113073. [Q96Q91-1]
GeneID83697.
KEGGhsa:83697.
UCSCuc003lfj.2. human. [Q96Q91-3]
uc003lfk.2. human. [Q96Q91-2]
uc003lfm.3. human. [Q96Q91-1]

Organism-specific databases

CTD83697.
GeneCardsGC05P139719.
H-InvDBHIX0031939.
HGNCHGNC:11035. SLC4A9.
HPAHPA051307.
MIM610207. gene.
neXtProtNX_Q96Q91.
PharmGKBPA35901.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG301727.
HOVERGENHBG004326.
InParanoidQ96Q91.
KOK13860.
OMAQELLWLD.
OrthoDBEOG7TMZR0.
PhylomeDBQ96Q91.
TreeFamTF313630.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

BgeeQ96Q91.
CleanExHS_SLC4A9.
GenevestigatorQ96Q91.

Family and domain databases

Gene3D3.40.1100.10. 1 hit.
InterProIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PfamPF07565. Band_3_cyto. 2 hits.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMSSF55804. SSF55804. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi83697.
NextBio72674.
PROQ96Q91.
SOURCESearch...

Entry information

Entry nameB3A4_HUMAN
AccessionPrimary (citable) accession number: Q96Q91
Secondary accession number(s): B7ZL63 expand/collapse secondary AC list , D3DQD4, D3DQD5, D3DQD6, E9PDK1, Q96RM5, Q9BXF2, Q9BXN3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM