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Protein

Ankyrin repeat and SOCS box protein 2

Gene

ASB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.2 Publications
Isoform 1: Enhances adhesion of hematopoietic cells to fibronectin through targeting of filamins FLNA and FLNB for proteasomal degradation, it is transiantly expressed during hematopoietic cell differentiation.2 Publications
Isoform 2: Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100628-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SOCS box protein 2
Short name:
ASB-2
Gene namesi
Name:ASB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:16012. ASB2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi548L → P: No interaction with Elongin BC complex. 1 Publication1
Mutagenesisi551 – 552LC → PF: No interaction with CUL5 or RNF7. 1 Publication2
Mutagenesisi552C → P: No interaction with Elongin BC complex. 1 Publication1
Mutagenesisi571 – 574LPLP → AAAA: No interaction with CUL5 or RNF7. 1 Publication4

Organism-specific databases

DisGeNETi51676.
OpenTargetsiENSG00000100628.
ENSG00000278693.
PharmGKBiPA25030.

Polymorphism and mutation databases

BioMutaiASB2.
DMDMi20531999.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669251 – 587Ankyrin repeat and SOCS box protein 2Add BLAST587

Proteomic databases

PaxDbiQ96Q27.
PeptideAtlasiQ96Q27.
PRIDEiQ96Q27.

PTM databases

iPTMnetiQ96Q27.
PhosphoSitePlusiQ96Q27.

Expressioni

Inductioni

By all-trans retinoic acid (ATRA).2 Publications

Gene expression databases

BgeeiENSG00000100628.
CleanExiHS_ASB2.
ExpressionAtlasiQ96Q27. baseline and differential.
GenevisibleiQ96Q27. HS.

Organism-specific databases

HPAiHPA001546.

Interactioni

Subunit structurei

Component of a probable ECS E3 ubiquitin-protein ligase complex which contains CUL5, either RBX1 or RNF7/RBX2, Elongin BC complex (TCEB1 and TCEB2) and ASB2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CUL5Q930344EBI-2880677,EBI-1057139
RNF7Q9UBF62EBI-2880677,EBI-398632

Protein-protein interaction databases

BioGridi119674. 39 interactors.
IntActiQ96Q27. 5 interactors.
MINTiMINT-1187414.
STRINGi9606.ENSP00000451575.

Structurei

3D structure databases

ProteinModelPortaliQ96Q27.
SMRiQ96Q27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati56 – 85ANK 1Add BLAST30
Repeati89 – 119ANK 2Add BLAST31
Repeati123 – 152ANK 3Add BLAST30
Repeati156 – 185ANK 4Add BLAST30
Repeati189 – 218ANK 5Add BLAST30
Repeati222 – 251ANK 6Add BLAST30
Repeati255 – 284ANK 7Add BLAST30
Repeati288 – 317ANK 8Add BLAST30
Repeati320 – 349ANK 9Add BLAST30
Repeati362 – 391ANK 10Add BLAST30
Repeati392 – 421ANK 11Add BLAST30
Repeati428 – 456ANK 12Add BLAST29
Domaini533 – 587SOCS boxPROSITE-ProRule annotationAdd BLAST55

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 16Required for interaction with FLNA1 Publication9

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.By similarity
Both the N-terminus and ANK repeats 1 to 10 are necessary for interaction with filamins.1 Publication

Sequence similaritiesi

Belongs to the ankyrin SOCS box (ASB) family.Curated
Contains 12 ANK repeats.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063050.
HOGENOMiHOG000034086.
HOVERGENiHBG104037.
InParanoidiQ96Q27.
KOiK10324.
OMAiNADTNHR.
PhylomeDBiQ96Q27.
TreeFamiTF315127.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF13606. Ank_3. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 11 hits.
SM00253. SOCS. 1 hit.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 9 hits.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96Q27-1) [UniParc]FASTAAdd to basket
Also known as: ASB2alpha, hematopoietic-type1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTRFSYAEYF SLFHSCSAPS RSTAPPESSP ARAPMGLFQG VMQKYSSSLF
60 70 80 90 100
KTSQLAPADP LIKAIKDGDE EALKTMIKEG KNLAEPNKEG WLPLHEAAYY
110 120 130 140 150
GQVGCLKVLQ RAYPGTIDQR TLQEETAVYL ATCRGHLDCL LSLLQAGAEP
160 170 180 190 200
DISNKSRETP LYKACERKNA EAVKILVQHN ADTNHRCNRG WTALHESVSR
210 220 230 240 250
NDLEVMQILV SGGAKVESKN AYGITPLFVA AQSGQLEALR FLAKYGADIN
260 270 280 290 300
TQASDNASAL YEACKNEHEE VVEFLLSQGA DANKTNKDGL LPLHIASKKG
310 320 330 340 350
NYRIVQMLLP VTSRTRIRRS GVSPLHLAAE RNHDEVLEAL LSARFDVNTP
360 370 380 390 400
LAPERARLYE DRRSSALYFA VVNNNVYATE LLLQHGADPN RDVISPLLVA
410 420 430 440 450
IRHGCLRTMQ LLLDHGANID AYIATHPTAF PATIMFAMKC LSLLKFLMDL
460 470 480 490 500
GCDGEPCFSC LYGNGPHPPA PQPSSRFNDA PAADKEPSVV QFCEFVSAPE
510 520 530 540 550
VSRWAGPIID VLLDYVGNVQ LCSRLKEHID SFEDWAVIKE KAEPPRPLAH
560 570 580
LCRLRVRKAI GKYRIKLLDT LPLPGRLIRY LKYENTQ
Length:587
Mass (Da):65,084
Last modified:December 1, 2001 - v1
Checksum:i866A2B6010C4C1F9
GO
Isoform 2 (identifier: Q96Q27-2) [UniParc]FASTAAdd to basket
Also known as: ASB2beta, muscle-type1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MTRFSYAEYFSLFHSCSAPS → MATQISTRGS...RQPAHFYPWT

Note: Contains a UIM domain at positions 26-41.
Show »
Length:635
Mass (Da):70,212
Checksum:iB91E4EB394531076
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224Missing in AAD45345 (PubMed:11111040).Curated1
Sequence conflicti460Missing in AAD45345 (PubMed:11111040).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022089160P → S.Corresponds to variant rs2295213dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0430421 – 20MTRFS…CSAPS → MATQISTRGSQCTIGQEEYS LYSSLSEDELVQMAIEQSLA DKTRGPTTAEATASACTNRQ PAHFYPWT in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056723 mRNA. Translation: BAB64532.1.
AJ251238 mRNA. Translation: CAC17765.1.
AB488462 mRNA. Translation: BAI77868.1.
AK303686 mRNA. Translation: BAG64678.1.
AK315628 mRNA. Translation: BAG37996.1.
AL079302 Genomic DNA. No translation available.
AL132642 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81540.1.
CH471061 Genomic DNA. Translation: EAW81542.1.
BC032354 mRNA. Translation: AAH32354.1.
AL137735 mRNA. Translation: CAB70899.1.
AF159164 mRNA. Translation: AAD45345.1.
CCDSiCCDS55940.1. [Q96Q27-2]
CCDS9915.1. [Q96Q27-1]
PIRiT46507.
RefSeqiNP_001189358.1. NM_001202429.1. [Q96Q27-2]
NP_057234.2. NM_016150.4. [Q96Q27-1]
XP_005267815.1. XM_005267758.3. [Q96Q27-2]
UniGeneiHs.510327.

Genome annotation databases

EnsembliENST00000315988; ENSP00000320675; ENSG00000100628. [Q96Q27-1]
ENST00000555019; ENSP00000451575; ENSG00000100628. [Q96Q27-2]
ENST00000612647; ENSP00000480388; ENSG00000278693. [Q96Q27-2]
ENST00000629617; ENSP00000487399; ENSG00000278693. [Q96Q27-1]
GeneIDi51676.
KEGGihsa:51676.
UCSCiuc001ycc.3. human. [Q96Q27-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056723 mRNA. Translation: BAB64532.1.
AJ251238 mRNA. Translation: CAC17765.1.
AB488462 mRNA. Translation: BAI77868.1.
AK303686 mRNA. Translation: BAG64678.1.
AK315628 mRNA. Translation: BAG37996.1.
AL079302 Genomic DNA. No translation available.
AL132642 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81540.1.
CH471061 Genomic DNA. Translation: EAW81542.1.
BC032354 mRNA. Translation: AAH32354.1.
AL137735 mRNA. Translation: CAB70899.1.
AF159164 mRNA. Translation: AAD45345.1.
CCDSiCCDS55940.1. [Q96Q27-2]
CCDS9915.1. [Q96Q27-1]
PIRiT46507.
RefSeqiNP_001189358.1. NM_001202429.1. [Q96Q27-2]
NP_057234.2. NM_016150.4. [Q96Q27-1]
XP_005267815.1. XM_005267758.3. [Q96Q27-2]
UniGeneiHs.510327.

3D structure databases

ProteinModelPortaliQ96Q27.
SMRiQ96Q27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119674. 39 interactors.
IntActiQ96Q27. 5 interactors.
MINTiMINT-1187414.
STRINGi9606.ENSP00000451575.

PTM databases

iPTMnetiQ96Q27.
PhosphoSitePlusiQ96Q27.

Polymorphism and mutation databases

BioMutaiASB2.
DMDMi20531999.

Proteomic databases

PaxDbiQ96Q27.
PeptideAtlasiQ96Q27.
PRIDEiQ96Q27.

Protocols and materials databases

DNASUi51676.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315988; ENSP00000320675; ENSG00000100628. [Q96Q27-1]
ENST00000555019; ENSP00000451575; ENSG00000100628. [Q96Q27-2]
ENST00000612647; ENSP00000480388; ENSG00000278693. [Q96Q27-2]
ENST00000629617; ENSP00000487399; ENSG00000278693. [Q96Q27-1]
GeneIDi51676.
KEGGihsa:51676.
UCSCiuc001ycc.3. human. [Q96Q27-1]

Organism-specific databases

CTDi51676.
DisGeNETi51676.
GeneCardsiASB2.
HGNCiHGNC:16012. ASB2.
HPAiHPA001546.
MIMi605759. gene.
neXtProtiNX_Q96Q27.
OpenTargetsiENSG00000100628.
ENSG00000278693.
PharmGKBiPA25030.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063050.
HOGENOMiHOG000034086.
HOVERGENiHBG104037.
InParanoidiQ96Q27.
KOiK10324.
OMAiNADTNHR.
PhylomeDBiQ96Q27.
TreeFamiTF315127.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000100628-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GeneWikiiASB2.
GenomeRNAii51676.
PROiQ96Q27.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100628.
CleanExiHS_ASB2.
ExpressionAtlasiQ96Q27. baseline and differential.
GenevisibleiQ96Q27. HS.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF13606. Ank_3. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 11 hits.
SM00253. SOCS. 1 hit.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 9 hits.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASB2_HUMAN
AccessioniPrimary (citable) accession number: Q96Q27
Secondary accession number(s): B2RDP9
, B4E166, Q9NSU5, Q9Y567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.