Reviewed, UniProtKB/Swiss-Prot Q96Q15 (SMG1_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SMG1
      Short name=SMG-1
      Short name=hSMG-1
    EC=2.7.11.1
Alternative name(s):
    Lambda/iota protein kinase C-interacting protein
      Short name=Lambda-interacting protein
    61E3.4

Gene names

Name:	SMG1
Synonyms:	ATX, KIAA0421, LIP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	3657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Acts as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, it phosphorylates UPF1/RENT1, triggering nonsense-mediated decay (NMD). Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate TP53/p53 and is required for optimal TP53/p53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. Ref.2 Ref.3 Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Manganese. Ref.3
Enzyme regulation	Inhibited by caffeine, LY294002 and wortmannin. Ref.2 Ref.3 Ref.4
Subunit structure	Component of a post-splicing multiprotein NMD complex with UPF1/RENT1, UPF2/RENT2, EST1A (SMG5/7a), EST1B/SMG5 and UPF3 (UPF3A or UPF3B). Component of the SMG1C complex, at least composed of SMG1, SMG8 and SMG9. The SMG1C complex is then recruited on premature translation termination codons (PTCs) to form the ribosome:SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with PRKCI. Ref.2 Ref.7 Ref.9 Ref.10
Subcellular location	Nucleus. Cytoplasm. Ref.4
Tissue specificity	Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. Ref.4 Ref.7
Post-translational modification	Autophosphorylated Probable. Phosphorylated upon DNA damage, probably by ATM or ATR.
Miscellaneous	This gene is located in a region of chromosome 16 that contains 2 segmental duplications. Other genes that are highly related to this exist, but they probably represent pseudogenes.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 HEAT repeat. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Nonsense-mediated mRNA decay
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW mRNA export from nucleus Traceable author statement. Source: UniProtKB nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Ref.2 Ref.3 Inferred from direct assay. Source: UniProtKB peptidyl-serine phosphorylation Ref.2 Ref.4 Ref.9 Inferred from direct assay. Source: UniProtKB phosphoinositide phosphorylation Ref.3 Inferred from direct assay. Source: UniProtKB protein amino acid autophosphorylation Ref.2 Ref.3 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.3 Ref.4 Ref.9 Inferred from direct assay. Source: UniProtKB nucleus Ref.4 Ref.9 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.2 Ref.14 Inferred from physical interaction. Source: UniProtKB protein serine/threonine kinase activity Ref.2 Ref.3 Ref.4 Ref.14 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96Q15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96Q15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Isoform 3 (identifier: Q96Q15-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-626: Missing.

Isoform 4 (identifier: Q96Q15-4)

Also known as: BLIP;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1265: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 3657	3657	Serine/threonine-protein kinase SMG1		PRO_0000229791
Regions
	Domain	1127 – 1862	736	FAT
	Repeat	1813 – 1848	36	HEAT
	Domain	2146 – 2474	329	PI3K/PI4K
	Domain	3625 – 3657	33	FATC
Amino acid modifications
	Modified residue	3546	1	Phosphothreonine Ref.11
	Modified residue	3552	1	Phosphoserine Ref.11
	Modified residue	3566	1	Phosphoserine Ref.12
	Modified residue	3572	1	Phosphoserine Ref.12
	Modified residue	3573	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 1265	1265	Missing in isoform 4.		VSP_017746
	Alternative sequence	1 – 626	626	Missing in isoform 3.		VSP_017747
	Alternative sequence	1 – 136	136	Missing in isoform 2.		VSP_017748
	Natural variant	31	1	A → T Ref.15		VAR_041623
	Natural variant	122	1	R → C Ref.15		VAR_041624
	Natural variant	140	1	S → C Ref.4 Ref.15		VAR_041625
	Natural variant	147	1	N → Y Ref.15		VAR_041626
	Natural variant	156	1	D → N Ref.15		VAR_041627
	Natural variant	163	1	A → V Ref.15		VAR_041628
	Natural variant	316	1	D → G Ref.15		VAR_041629
	Natural variant	461	1	G → S Ref.15		VAR_041630
	Natural variant	542	1	H → R Ref.15		VAR_041631
	Natural variant	584	1	A → S Ref.15		VAR_041632
	Natural variant	608	1	K → I Ref.4 Ref.15		VAR_041633
	Natural variant	749	1	S → C Ref.15		VAR_041634
	Natural variant	805	1	S → C Ref.15		VAR_041635
	Natural variant	808	1	R → C Ref.15		VAR_041636
	Natural variant	825	1	V → I Ref.15		VAR_041637
	Natural variant	828	1	N → D Ref.15		VAR_041638
	Natural variant	948	1	A → G Ref.15		VAR_041639
	Natural variant	965	1	N → S Ref.15		VAR_041640
	Natural variant	1012	1	F → L Ref.15		VAR_041641
	Natural variant	1025	1	R → Q Ref.15		VAR_041642
	Natural variant	1068	1	T → S Ref.15		VAR_041643
	Natural variant	1099	1	N → H Ref.15		VAR_041644
	Natural variant	1271	1	P → R Ref.15		VAR_041645
	Natural variant	1288	1	Q → P Ref.15		VAR_041646
	Natural variant	1328	1	I → V Ref.15		VAR_041647
	Natural variant	1354	1	S → P Ref.15		VAR_041648
	Natural variant	1414	1	R → T Ref.15		VAR_041649
	Natural variant	2167	1	S → C in a breast pleomorphic lobular carcinoma sample; somatic mutation. Ref.15		VAR_041650
	Natural variant	2254	1	G → S Ref.15		VAR_041651
	Natural variant	2341	1	M → K Ref.15		VAR_041652
	Natural variant	2726	1	Q → E Ref.15		VAR_041653
	Natural variant	2885	1	G → S Ref.15		VAR_041654
	Natural variant	2895	1	P → A Ref.15		VAR_041655
	Natural variant	3235	1	I → T in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.15		VAR_041656
	Natural variant	3579	1	K → Q in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.15		VAR_041657
Experimental info
	Mutagenesis	2331	1	D → A: Loss of function. Ref.2 Ref.3 Ref.4
	Sequence conflict	682	1	A → S Ref.3
	Sequence conflict	682	1	A → S Ref.4
	Sequence conflict	739	1	R → K Ref.3
	Sequence conflict	739	1	R → K Ref.4
	Sequence conflict	1189	1	F → C Ref.3
	Sequence conflict	1189	1	F → C Ref.4
	Sequence conflict	2005	1	K → R in BAB70696. Ref.2
	Sequence conflict	2073	1	S → N in AAK58892. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 4, 2006. Version 2. Checksum: ADBBEA902F6B7DEA	FASTA	3,657	410,261
	10 20 30 40 50 60 MSRRAPGSRL SSGGTNYSRS WNDWQPRTDS ASADPGNLKY SSSRDRGGSS SYGLQPSNSA 70 80 90 100 110 120 VVSRQRHDDT RVHADIQNDE KGGYSVNGGS GENTYGRKSL GQELRVNNVT SPEFTSVQHG 130 140 150 160 170 180 SRALATKDMR KSQERSMSYS DESRLSNLLR RITREDDRDR RLATVKQLKE FIQQPENKLV 190 200 210 220 230 240 LVKQLDNILA AVHDVLNESS KLLQELRQEG ACCLGLLCAS LSYEAEKIFK WIFSKFSSSA 250 260 270 280 290 300 KDEVKLLYLC ATYKALETVG EKKAFSSVMQ LVMTSLQSIL ENVDTPELLC KCVKCILLVA 310 320 330 340 350 360 RCYPHIFSTN FRDTVDILVG WHIDHTQKPS LTQQVSGWLQ SLEPFWVADL AFSTTLLGQF 370 380 390 400 410 420 LEDMEAYAED LSHVASGESV DEDVPPPSVS LPKLAALLRV FSTVVRSIGE RFSPIRGPPI 430 440 450 460 470 480 TEAYVTDVLY RVMRCVTAAN QVFFSEAVLT AANECVGVLL GSLDPSMTIH CDMVITYGLD 490 500 510 520 530 540 QLENCQTCGT DYIISVLNLL TLIVEQINTK LPSSFVEKLF IPSSKLLFLR YHKEKEVVAV 550 560 570 580 590 600 AHAVYQAVLS LKNIPVLETA YKLILGEMTC ALNNLLHSLQ LPEACSEIKH EAFKNHVFNV 610 620 630 640 650 660 DNAKFVVKFD LSALTTIGNA KNSLIGMWAL SPTVFALLSK NLMIVHSDLA VHFPAIQYAV 670 680 690 700 710 720 LYTLYSHCTR HDHFISSSLS SASPSLFDGA VISTVTTATK KHFSIILNLL GILLKKDNLN 730 740 750 760 770 780 QDTRKLLMTW ALEAAVLMRK SETYAPLFSL PSFHKFCKGL LANTLVEDVN ICLQACSSLH 790 800 810 820 830 840 ALSSSLPDDL LQRCVDVCRV QLVHSGTRIR QAFGKLLKSI PLDVVLSNNN HTEIQEISLA 850 860 870 880 890 900 LRSHMSKAPS NTFHPQDFSD VISFILYGNS HRTGKDNWLE RLFYSCQRLD KRDQSTIPRN 910 920 930 940 950 960 LLKTDAVLWQ WAIWEAAQFT VLSKLRTPLG RAQDTFQTIE GIIRSLAAHT LNPDQDVSQW 970 980 990 1000 1010 1020 TTADNDEGHG NNQLRLVLLL QYLENLEKLM YNAYEGCANA LTSPPKVIRT FFYTNRQTCQ 1030 1040 1050 1060 1070 1080 DWLTRIRLSI MRVGLLAGQP AVTVRHGFDL LTEMKTTSLS QGNELEVTIM MVVEALCELH 1090 1100 1110 1120 1130 1140 CPEAIQGIAV WSSSIVGKNL LWINSVAQQA EGRFEKASVE YQEHLCAMTG VDCCISSFDK 1150 1160 1170 1180 1190 1200 SVLTLANAGR NSASPKHSLN GESRKTVLSK PTDSSPEVIN YLGNKACEFY ISIADWAAVQ 1210 1220 1230 1240 1250 1260 EWQNAIHDLK KSTSSTSLNL KADFNYIKSL SSFESGKFVE CTEQLELLPG ENINLLAGGS 1270 1280 1290 1300 1310 1320 KEKIDMKKLL PNMLSPDPRE LQKSIEVQLL RSSVCLATAL NPIEQDQKWQ SITENVVKYL 1330 1340 1350 1360 1370 1380 KQTSRIAIGP LRLSTLTVSQ SLPVLSTLQL YCSSALENTV SNRLSTEDCL IPLFSEALRS 1390 1400 1410 1420 1430 1440 CKQHDVRPWM QALRYTMYQN QLLEKIKEQT VPIRSHLMEL GLTAAKFARK RGNVSLATRL 1450 1460 1470 1480 1490 1500 LAQCSEVQLG KTTTAQDLVQ HFKKLSTQGQ VDEKWGPELD IEKTKLLYTA GQSTHAMEML 1510 1520 1530 1540 1550 1560 SSCAISFCKS VKAEYAVAKS ILTLAKWIQA EWKEISGQLK QVYRAQHQQN FTGLSTLSKN 1570 1580 1590 1600 1610 1620 ILTLIELPSV NTMEEEYPRI ESESTVHIGV GEPDFILGQL YHLSSVQAPE VAKSWAALAS 1630 1640 1650 1660 1670 1680 WAYRWGRKVV DNASQGEGVR LLPREKSEVQ NLLPDTITEE EKERIYGILG QAVCRPAGIQ 1690 1700 1710 1720 1730 1740 DEDITLQITE SEDNEEDDMV DVIWRQLISS CPWLSELDES ATEGVIKVWR KVVDRIFSLY 1750 1760 1770 1780 1790 1800 KLSCSAYFTF LKLNAGQIPL DEDDPRLHLS HRVEQSTDDM IVMATLRLLR LLVKHAGELR 1810 1820 1830 1840 1850 1860 QYLEHGLETT PTAPWRGIIP QLFSRLNHPE VYVRQSICNL LCRVAQDSPH LILYPAIVGT 1870 1880 1890 1900 1910 1920 ISLSSESQAS GNKFSTAIPT LLGNIQGEEL LVSECEGGSP PASQDSNKDE PKSGLNEDQA 1930 1940 1950 1960 1970 1980 MMQDCYSKIV DKLSSANPTM VLQVQMLVAE LRRVTVLWDE LWLGVLLQQH MYVLRRIQQL 1990 2000 2010 2020 2030 2040 EDEVKRVQNN NTLRKEEKIA IMREKHTALM KPIVFALEHV RSITAAPAET PHEKWFQDNY 2050 2060 2070 2080 2090 2100 GDAIENALEK LKTPLNPAKP GSSWIPFKEI MLSLQQRAQK RASYILRLEE ISPWLAAMTN 2110 2120 2130 2140 2150 2160 TEIALPGEVS ARDTVTIHSV GGTITILPTK TKPKKLLFLG SDGKSYPYLF KGLEDLHLDE 2170 2180 2190 2200 2210 2220 RIMQFLSIVN TMFATINRQE TPRFHARHYS VTPLGTRSGL IQWVDGATPL FGLYKRWQQR 2230 2240 2250 2260 2270 2280 EAALQAQKAQ DSYQTPQNPG IVPRPSELYY SKIGPALKTV GLSLDVSRRD WPLHVMKAVL 2290 2300 2310 2320 2330 2340 EELMEATPPN LLAKELWSSC TTPDEWWRVT QSYARSTAVM SMVGYIIGLG DRHLDNVLID 2350 2360 2370 2380 2390 2400 MTTGEVVHID YNVCFEKGKS LRVPEKVPFR MTQNIETALG VTGVEGVFRL SCEQVLHIMR 2410 2420 2430 2440 2450 2460 RGRETLLTLL EAFVYDPLVD WTAGGEAGFA GAVYGGGGQQ AESKQSKREM EREITRSLFS 2470 2480 2490 2500 2510 2520 SRVAEIKVNW FKNRDEMLVV LPKLDGSLDE YLSLQEQLTD VEKLQGKLLE EIEFLEGAEG 2530 2540 2550 2560 2570 2580 VDHPSHTLQH RYSEHTQLQT QQRAVQEAIQ VKLNEFEQWI THYQAAFNNL EATQLASLLQ 2590 2600 2610 2620 2630 2640 EISTQMDLGP PSYVPATAFL QNAGQAHLIS QCEQLEGEVG ALLQQRRSVL RGCLEQLHHY 2650 2660 2670 2680 2690 2700 ATVALQYPKA IFQKHRIEQW KTWMEELICN TTVERCQELY RKYEMQYAPQ PPPTVCQFIT 2710 2720 2730 2740 2750 2760 ATEMTLQRYA ADINSRLIRQ VERLKQEAVT VPVCEDQLKE IERCIKVFLH ENGEEGSLSL 2770 2780 2790 2800 2810 2820 ASVIISALCT LTRRNLMMEG AASSAGEQLV DLTSRDGAWF LEELCSMSGN VTCLVQLLKQ 2830 2840 2850 2860 2870 2880 CHLVPQDLDI PNPMEASETV HLANGVYTSL QELNSNFRQI IFPEALRCLM KGEYTLESML 2890 2900 2910 2920 2930 2940 HELDGLIEQT TDGVPLQTLV ESLQAYLRNA AMGLEEETHA HYIDVARLLH AQYGELIQPR 2950 2960 2970 2980 2990 3000 NGSVDETPKM SAGQMLLVAF DGMFAQVETA FSLLVEKLNK MEIPIAWRKI DIIREARSTQ 3010 3020 3030 3040 3050 3060 VNFFDDDNHR QVLEEIFFLK RLQTIKEFFR LCGTFSKTLS GSSSLEDQNT VNGPVQIVNV 3070 3080 3090 3100 3110 3120 KTLFRNSCFS EDQMAKPIKA FTADFVRQLL IGLPNQALGL TLCSFISALG VDIIAQVEAK 3130 3140 3150 3160 3170 3180 DFGAESKVSV DDLCKKAVEH NIQIGKFSQL VMNRATVLAS SYDTAWKKHD LVRRLETSIS 3190 3200 3210 3220 3230 3240 SCKTSLQRVQ LHIAMFQWQH EDLLINRPQA MSVTPPPRSA ILTSMKKKLH TLSQIETSIA 3250 3260 3270 3280 3290 3300 TVQEKLAALE SSIEQRLKWA GGANPALAPV LQDFEATIAE RRNLVLKESQ RASQVTFLCS 3310 3320 3330 3340 3350 3360 NIIHFESLRT RTAEALNLDA ALFELIKRCQ QMCSFASQFN SSVSELELRL LQRVDTGLEH 3370 3380 3390 3400 3410 3420 PIGSSEWLLS AHKQLTQDMS TQRAIQTEKE QQIETVCETI QNLVDNIKTV LTGHNRQLGD 3430 3440 3450 3460 3470 3480 VKHLLKAMAK DEEAALADGE DVPYENSVRQ FLGEYKSWQD NIQTVLFTLV QAMGQVRSQE 3490 3500 3510 3520 3530 3540 HVEMLQEITP TLKELKTQSQ SIYNNLVSFA SPLVTDATNE CSSPTSSATY QPSFAAAVRS 3550 3560 3570 3580 3590 3600 NTGQKTQPDV MSQNARKLIQ KNLATSADTP PSTVPGTGKS VACSPKKAVR DPKTGKAVQE 3610 3620 3630 3640 3650 RNSYAVSVWK RVKAKLEGRD VDPNRRMSVA EQVDYVIKEA TNLDNLAQLY EGWTAWV
	Isoform 2. Checksum: 1AE916BA42B724F6	FASTA	3,521	395,453
	10 20 30 40 50 60 MSYSDESRLS NLLRRITRED DRDRRLATVK QLKEFIQQPE NKLVLVKQLD NILAAVHDVL 70 80 90 100 110 120 NESSKLLQEL RQEGACCLGL LCASLSYEAE KIFKWIFSKF SSSAKDEVKL LYLCATYKAL 130 140 150 160 170 180 ETVGEKKAFS SVMQLVMTSL QSILENVDTP ELLCKCVKCI LLVARCYPHI FSTNFRDTVD 190 200 210 220 230 240 ILVGWHIDHT QKPSLTQQVS GWLQSLEPFW VADLAFSTTL LGQFLEDMEA YAEDLSHVAS 250 260 270 280 290 300 GESVDEDVPP PSVSLPKLAA LLRVFSTVVR SIGERFSPIR GPPITEAYVT DVLYRVMRCV 310 320 330 340 350 360 TAANQVFFSE AVLTAANECV GVLLGSLDPS MTIHCDMVIT YGLDQLENCQ TCGTDYIISV 370 380 390 400 410 420 LNLLTLIVEQ INTKLPSSFV EKLFIPSSKL LFLRYHKEKE VVAVAHAVYQ AVLSLKNIPV 430 440 450 460 470 480 LETAYKLILG EMTCALNNLL HSLQLPEACS EIKHEAFKNH VFNVDNAKFV VKFDLSALTT 490 500 510 520 530 540 IGNAKNSLIG MWALSPTVFA LLSKNLMIVH SDLAVHFPAI QYAVLYTLYS HCTRHDHFIS 550 560 570 580 590 600 SSLSSASPSL FDGAVISTVT TATKKHFSII LNLLGILLKK DNLNQDTRKL LMTWALEAAV 610 620 630 640 650 660 LMRKSETYAP LFSLPSFHKF CKGLLANTLV EDVNICLQAC SSLHALSSSL PDDLLQRCVD 670 680 690 700 710 720 VCRVQLVHSG TRIRQAFGKL LKSIPLDVVL SNNNHTEIQE ISLALRSHMS KAPSNTFHPQ 730 740 750 760 770 780 DFSDVISFIL YGNSHRTGKD NWLERLFYSC QRLDKRDQST IPRNLLKTDA VLWQWAIWEA 790 800 810 820 830 840 AQFTVLSKLR TPLGRAQDTF QTIEGIIRSL AAHTLNPDQD VSQWTTADND EGHGNNQLRL 850 860 870 880 890 900 VLLLQYLENL EKLMYNAYEG CANALTSPPK VIRTFFYTNR QTCQDWLTRI RLSIMRVGLL 910 920 930 940 950 960 AGQPAVTVRH GFDLLTEMKT TSLSQGNELE VTIMMVVEAL CELHCPEAIQ GIAVWSSSIV 970 980 990 1000 1010 1020 GKNLLWINSV AQQAEGRFEK ASVEYQEHLC AMTGVDCCIS SFDKSVLTLA NAGRNSASPK 1030 1040 1050 1060 1070 1080 HSLNGESRKT VLSKPTDSSP EVINYLGNKA CEFYISIADW AAVQEWQNAI HDLKKSTSST 1090 1100 1110 1120 1130 1140 SLNLKADFNY IKSLSSFESG KFVECTEQLE LLPGENINLL AGGSKEKIDM KKLLPNMLSP 1150 1160 1170 1180 1190 1200 DPRELQKSIE VQLLRSSVCL ATALNPIEQD QKWQSITENV VKYLKQTSRI AIGPLRLSTL 1210 1220 1230 1240 1250 1260 TVSQSLPVLS TLQLYCSSAL ENTVSNRLST EDCLIPLFSE ALRSCKQHDV RPWMQALRYT 1270 1280 1290 1300 1310 1320 MYQNQLLEKI KEQTVPIRSH LMELGLTAAK FARKRGNVSL ATRLLAQCSE VQLGKTTTAQ 1330 1340 1350 1360 1370 1380 DLVQHFKKLS TQGQVDEKWG PELDIEKTKL LYTAGQSTHA MEMLSSCAIS FCKSVKAEYA 1390 1400 1410 1420 1430 1440 VAKSILTLAK WIQAEWKEIS GQLKQVYRAQ HQQNFTGLST LSKNILTLIE LPSVNTMEEE 1450 1460 1470 1480 1490 1500 YPRIESESTV HIGVGEPDFI LGQLYHLSSV QAPEVAKSWA ALASWAYRWG RKVVDNASQG 1510 1520 1530 1540 1550 1560 EGVRLLPREK SEVQNLLPDT ITEEEKERIY GILGQAVCRP AGIQDEDITL QITESEDNEE 1570 1580 1590 1600 1610 1620 DDMVDVIWRQ LISSCPWLSE LDESATEGVI KVWRKVVDRI FSLYKLSCSA YFTFLKLNAG 1630 1640 1650 1660 1670 1680 QIPLDEDDPR LHLSHRVEQS TDDMIVMATL RLLRLLVKHA GELRQYLEHG LETTPTAPWR 1690 1700 1710 1720 1730 1740 GIIPQLFSRL NHPEVYVRQS ICNLLCRVAQ DSPHLILYPA IVGTISLSSE SQASGNKFST 1750 1760 1770 1780 1790 1800 AIPTLLGNIQ GEELLVSECE GGSPPASQDS NKDEPKSGLN EDQAMMQDCY SKIVDKLSSA 1810 1820 1830 1840 1850 1860 NPTMVLQVQM LVAELRRVTV LWDELWLGVL LQQHMYVLRR IQQLEDEVKR VQNNNTLRKE 1870 1880 1890 1900 1910 1920 EKIAIMREKH TALMKPIVFA LEHVRSITAA PAETPHEKWF QDNYGDAIEN ALEKLKTPLN 1930 1940 1950 1960 1970 1980 PAKPGSSWIP FKEIMLSLQQ RAQKRASYIL RLEEISPWLA AMTNTEIALP GEVSARDTVT 1990 2000 2010 2020 2030 2040 IHSVGGTITI LPTKTKPKKL LFLGSDGKSY PYLFKGLEDL HLDERIMQFL SIVNTMFATI 2050 2060 2070 2080 2090 2100 NRQETPRFHA RHYSVTPLGT RSGLIQWVDG ATPLFGLYKR WQQREAALQA QKAQDSYQTP 2110 2120 2130 2140 2150 2160 QNPGIVPRPS ELYYSKIGPA LKTVGLSLDV SRRDWPLHVM KAVLEELMEA TPPNLLAKEL 2170 2180 2190 2200 2210 2220 WSSCTTPDEW WRVTQSYARS TAVMSMVGYI IGLGDRHLDN VLIDMTTGEV VHIDYNVCFE 2230 2240 2250 2260 2270 2280 KGKSLRVPEK VPFRMTQNIE TALGVTGVEG VFRLSCEQVL HIMRRGRETL LTLLEAFVYD 2290 2300 2310 2320 2330 2340 PLVDWTAGGE AGFAGAVYGG GGQQAESKQS KREMEREITR SLFSSRVAEI KVNWFKNRDE 2350 2360 2370 2380 2390 2400 MLVVLPKLDG SLDEYLSLQE QLTDVEKLQG KLLEEIEFLE GAEGVDHPSH TLQHRYSEHT 2410 2420 2430 2440 2450 2460 QLQTQQRAVQ EAIQVKLNEF EQWITHYQAA FNNLEATQLA SLLQEISTQM DLGPPSYVPA 2470 2480 2490 2500 2510 2520 TAFLQNAGQA HLISQCEQLE GEVGALLQQR RSVLRGCLEQ LHHYATVALQ YPKAIFQKHR 2530 2540 2550 2560 2570 2580 IEQWKTWMEE LICNTTVERC QELYRKYEMQ YAPQPPPTVC QFITATEMTL QRYAADINSR 2590 2600 2610 2620 2630 2640 LIRQVERLKQ EAVTVPVCED QLKEIERCIK VFLHENGEEG SLSLASVIIS ALCTLTRRNL 2650 2660 2670 2680 2690 2700 MMEGAASSAG EQLVDLTSRD GAWFLEELCS MSGNVTCLVQ LLKQCHLVPQ DLDIPNPMEA 2710 2720 2730 2740 2750 2760 SETVHLANGV YTSLQELNSN FRQIIFPEAL RCLMKGEYTL ESMLHELDGL IEQTTDGVPL 2770 2780 2790 2800 2810 2820 QTLVESLQAY LRNAAMGLEE ETHAHYIDVA RLLHAQYGEL IQPRNGSVDE TPKMSAGQML 2830 2840 2850 2860 2870 2880 LVAFDGMFAQ VETAFSLLVE KLNKMEIPIA WRKIDIIREA RSTQVNFFDD DNHRQVLEEI 2890 2900 2910 2920 2930 2940 FFLKRLQTIK EFFRLCGTFS KTLSGSSSLE DQNTVNGPVQ IVNVKTLFRN SCFSEDQMAK 2950 2960 2970 2980 2990 3000 PIKAFTADFV RQLLIGLPNQ ALGLTLCSFI SALGVDIIAQ VEAKDFGAES KVSVDDLCKK 3010 3020 3030 3040 3050 3060 AVEHNIQIGK FSQLVMNRAT VLASSYDTAW KKHDLVRRLE TSISSCKTSL QRVQLHIAMF 3070 3080 3090 3100 3110 3120 QWQHEDLLIN RPQAMSVTPP PRSAILTSMK KKLHTLSQIE TSIATVQEKL AALESSIEQR 3130 3140 3150 3160 3170 3180 LKWAGGANPA LAPVLQDFEA TIAERRNLVL KESQRASQVT FLCSNIIHFE SLRTRTAEAL 3190 3200 3210 3220 3230 3240 NLDAALFELI KRCQQMCSFA SQFNSSVSEL ELRLLQRVDT GLEHPIGSSE WLLSAHKQLT 3250 3260 3270 3280 3290 3300 QDMSTQRAIQ TEKEQQIETV CETIQNLVDN IKTVLTGHNR QLGDVKHLLK AMAKDEEAAL 3310 3320 3330 3340 3350 3360 ADGEDVPYEN SVRQFLGEYK SWQDNIQTVL FTLVQAMGQV RSQEHVEMLQ EITPTLKELK 3370 3380 3390 3400 3410 3420 TQSQSIYNNL VSFASPLVTD ATNECSSPTS SATYQPSFAA AVRSNTGQKT QPDVMSQNAR 3430 3440 3450 3460 3470 3480 KLIQKNLATS ADTPPSTVPG TGKSVACSPK KAVRDPKTGK AVQERNSYAV SVWKRVKAKL 3490 3500 3510 3520 EGRDVDPNRR MSVAEQVDYV IKEATNLDNL AQLYEGWTAW V
	Isoform 3. Checksum: 9ABE0AC258F26DB6	FASTA	3,031	340,730
	10 20 30 40 50 60 MWALSPTVFA LLSKNLMIVH SDLAVHFPAI QYAVLYTLYS HCTRHDHFIS SSLSSASPSL 70 80 90 100 110 120 FDGAVISTVT TATKKHFSII LNLLGILLKK DNLNQDTRKL LMTWALEAAV LMRKSETYAP 130 140 150 160 170 180 LFSLPSFHKF CKGLLANTLV EDVNICLQAC SSLHALSSSL PDDLLQRCVD VCRVQLVHSG 190 200 210 220 230 240 TRIRQAFGKL LKSIPLDVVL SNNNHTEIQE ISLALRSHMS KAPSNTFHPQ DFSDVISFIL 250 260 270 280 290 300 YGNSHRTGKD NWLERLFYSC QRLDKRDQST IPRNLLKTDA VLWQWAIWEA AQFTVLSKLR 310 320 330 340 350 360 TPLGRAQDTF QTIEGIIRSL AAHTLNPDQD VSQWTTADND EGHGNNQLRL VLLLQYLENL 370 380 390 400 410 420 EKLMYNAYEG CANALTSPPK VIRTFFYTNR QTCQDWLTRI RLSIMRVGLL AGQPAVTVRH 430 440 450 460 470 480 GFDLLTEMKT TSLSQGNELE VTIMMVVEAL CELHCPEAIQ GIAVWSSSIV GKNLLWINSV 490 500 510 520 530 540 AQQAEGRFEK ASVEYQEHLC AMTGVDCCIS SFDKSVLTLA NAGRNSASPK HSLNGESRKT 550 560 570 580 590 600 VLSKPTDSSP EVINYLGNKA CEFYISIADW AAVQEWQNAI HDLKKSTSST SLNLKADFNY 610 620 630 640 650 660 IKSLSSFESG KFVECTEQLE LLPGENINLL AGGSKEKIDM KKLLPNMLSP DPRELQKSIE 670 680 690 700 710 720 VQLLRSSVCL ATALNPIEQD QKWQSITENV VKYLKQTSRI AIGPLRLSTL TVSQSLPVLS 730 740 750 760 770 780 TLQLYCSSAL ENTVSNRLST EDCLIPLFSE ALRSCKQHDV RPWMQALRYT MYQNQLLEKI 790 800 810 820 830 840 KEQTVPIRSH LMELGLTAAK FARKRGNVSL ATRLLAQCSE VQLGKTTTAQ DLVQHFKKLS 850 860 870 880 890 900 TQGQVDEKWG PELDIEKTKL LYTAGQSTHA MEMLSSCAIS FCKSVKAEYA VAKSILTLAK 910 920 930 940 950 960 WIQAEWKEIS GQLKQVYRAQ HQQNFTGLST LSKNILTLIE LPSVNTMEEE YPRIESESTV 970 980 990 1000 1010 1020 HIGVGEPDFI LGQLYHLSSV QAPEVAKSWA ALASWAYRWG RKVVDNASQG EGVRLLPREK 1030 1040 1050 1060 1070 1080 SEVQNLLPDT ITEEEKERIY GILGQAVCRP AGIQDEDITL QITESEDNEE DDMVDVIWRQ 1090 1100 1110 1120 1130 1140 LISSCPWLSE LDESATEGVI KVWRKVVDRI FSLYKLSCSA YFTFLKLNAG QIPLDEDDPR 1150 1160 1170 1180 1190 1200 LHLSHRVEQS TDDMIVMATL RLLRLLVKHA GELRQYLEHG LETTPTAPWR GIIPQLFSRL 1210 1220 1230 1240 1250 1260 NHPEVYVRQS ICNLLCRVAQ DSPHLILYPA IVGTISLSSE SQASGNKFST AIPTLLGNIQ 1270 1280 1290 1300 1310 1320 GEELLVSECE GGSPPASQDS NKDEPKSGLN EDQAMMQDCY SKIVDKLSSA NPTMVLQVQM 1330 1340 1350 1360 1370 1380 LVAELRRVTV LWDELWLGVL LQQHMYVLRR IQQLEDEVKR VQNNNTLRKE EKIAIMREKH 1390 1400 1410 1420 1430 1440 TALMKPIVFA LEHVRSITAA PAETPHEKWF QDNYGDAIEN ALEKLKTPLN PAKPGSSWIP 1450 1460 1470 1480 1490 1500 FKEIMLSLQQ RAQKRASYIL RLEEISPWLA AMTNTEIALP GEVSARDTVT IHSVGGTITI 1510 1520 1530 1540 1550 1560 LPTKTKPKKL LFLGSDGKSY PYLFKGLEDL HLDERIMQFL SIVNTMFATI NRQETPRFHA 1570 1580 1590 1600 1610 1620 RHYSVTPLGT RSGLIQWVDG ATPLFGLYKR WQQREAALQA QKAQDSYQTP QNPGIVPRPS 1630 1640 1650 1660 1670 1680 ELYYSKIGPA LKTVGLSLDV SRRDWPLHVM KAVLEELMEA TPPNLLAKEL WSSCTTPDEW 1690 1700 1710 1720 1730 1740 WRVTQSYARS TAVMSMVGYI IGLGDRHLDN VLIDMTTGEV VHIDYNVCFE KGKSLRVPEK 1750 1760 1770 1780 1790 1800 VPFRMTQNIE TALGVTGVEG VFRLSCEQVL HIMRRGRETL LTLLEAFVYD PLVDWTAGGE 1810 1820 1830 1840 1850 1860 AGFAGAVYGG GGQQAESKQS KREMEREITR SLFSSRVAEI KVNWFKNRDE MLVVLPKLDG 1870 1880 1890 1900 1910 1920 SLDEYLSLQE QLTDVEKLQG KLLEEIEFLE GAEGVDHPSH TLQHRYSEHT QLQTQQRAVQ 1930 1940 1950 1960 1970 1980 EAIQVKLNEF EQWITHYQAA FNNLEATQLA SLLQEISTQM DLGPPSYVPA TAFLQNAGQA 1990 2000 2010 2020 2030 2040 HLISQCEQLE GEVGALLQQR RSVLRGCLEQ LHHYATVALQ YPKAIFQKHR IEQWKTWMEE 2050 2060 2070 2080 2090 2100 LICNTTVERC QELYRKYEMQ YAPQPPPTVC QFITATEMTL QRYAADINSR LIRQVERLKQ 2110 2120 2130 2140 2150 2160 EAVTVPVCED QLKEIERCIK VFLHENGEEG SLSLASVIIS ALCTLTRRNL MMEGAASSAG 2170 2180 2190 2200 2210 2220 EQLVDLTSRD GAWFLEELCS MSGNVTCLVQ LLKQCHLVPQ DLDIPNPMEA SETVHLANGV 2230 2240 2250 2260 2270 2280 YTSLQELNSN FRQIIFPEAL RCLMKGEYTL ESMLHELDGL IEQTTDGVPL QTLVESLQAY 2290 2300 2310 2320 2330 2340 LRNAAMGLEE ETHAHYIDVA RLLHAQYGEL IQPRNGSVDE TPKMSAGQML LVAFDGMFAQ 2350 2360 2370 2380 2390 2400 VETAFSLLVE KLNKMEIPIA WRKIDIIREA RSTQVNFFDD DNHRQVLEEI FFLKRLQTIK 2410 2420 2430 2440 2450 2460 EFFRLCGTFS KTLSGSSSLE DQNTVNGPVQ IVNVKTLFRN SCFSEDQMAK PIKAFTADFV 2470 2480 2490 2500 2510 2520 RQLLIGLPNQ ALGLTLCSFI SALGVDIIAQ VEAKDFGAES KVSVDDLCKK AVEHNIQIGK 2530 2540 2550 2560 2570 2580 FSQLVMNRAT VLASSYDTAW KKHDLVRRLE TSISSCKTSL QRVQLHIAMF QWQHEDLLIN 2590 2600 2610 2620 2630 2640 RPQAMSVTPP PRSAILTSMK KKLHTLSQIE TSIATVQEKL AALESSIEQR LKWAGGANPA 2650 2660 2670 2680 2690 2700 LAPVLQDFEA TIAERRNLVL KESQRASQVT FLCSNIIHFE SLRTRTAEAL NLDAALFELI 2710 2720 2730 2740 2750 2760 KRCQQMCSFA SQFNSSVSEL ELRLLQRVDT GLEHPIGSSE WLLSAHKQLT QDMSTQRAIQ 2770 2780 2790 2800 2810 2820 TEKEQQIETV CETIQNLVDN IKTVLTGHNR QLGDVKHLLK AMAKDEEAAL ADGEDVPYEN 2830 2840 2850 2860 2870 2880 SVRQFLGEYK SWQDNIQTVL FTLVQAMGQV RSQEHVEMLQ EITPTLKELK TQSQSIYNNL 2890 2900 2910 2920 2930 2940 VSFASPLVTD ATNECSSPTS SATYQPSFAA AVRSNTGQKT QPDVMSQNAR KLIQKNLATS 2950 2960 2970 2980 2990 3000 ADTPPSTVPG TGKSVACSPK KAVRDPKTGK AVQERNSYAV SVWKRVKAKL EGRDVDPNRR 3010 3020 3030 MSVAEQVDYV IKEATNLDNL AQLYEGWTAW V
	Isoform 4 (BLIP). Checksum: D63ECA30F812D7A6	FASTA	2,392	269,425
	10 20 30 40 50 60 MKKLLPNMLS PDPRELQKSI EVQLLRSSVC LATALNPIEQ DQKWQSITEN VVKYLKQTSR 70 80 90 100 110 120 IAIGPLRLST LTVSQSLPVL STLQLYCSSA LENTVSNRLS TEDCLIPLFS EALRSCKQHD 130 140 150 160 170 180 VRPWMQALRY TMYQNQLLEK IKEQTVPIRS HLMELGLTAA KFARKRGNVS LATRLLAQCS 190 200 210 220 230 240 EVQLGKTTTA QDLVQHFKKL STQGQVDEKW GPELDIEKTK LLYTAGQSTH AMEMLSSCAI 250 260 270 280 290 300 SFCKSVKAEY AVAKSILTLA KWIQAEWKEI SGQLKQVYRA QHQQNFTGLS TLSKNILTLI 310 320 330 340 350 360 ELPSVNTMEE EYPRIESEST VHIGVGEPDF ILGQLYHLSS VQAPEVAKSW AALASWAYRW 370 380 390 400 410 420 GRKVVDNASQ GEGVRLLPRE KSEVQNLLPD TITEEEKERI YGILGQAVCR PAGIQDEDIT 430 440 450 460 470 480 LQITESEDNE EDDMVDVIWR QLISSCPWLS ELDESATEGV IKVWRKVVDR IFSLYKLSCS 490 500 510 520 530 540 AYFTFLKLNA GQIPLDEDDP RLHLSHRVEQ STDDMIVMAT LRLLRLLVKH AGELRQYLEH 550 560 570 580 590 600 GLETTPTAPW RGIIPQLFSR LNHPEVYVRQ SICNLLCRVA QDSPHLILYP AIVGTISLSS 610 620 630 640 650 660 ESQASGNKFS TAIPTLLGNI QGEELLVSEC EGGSPPASQD SNKDEPKSGL NEDQAMMQDC 670 680 690 700 710 720 YSKIVDKLSS ANPTMVLQVQ MLVAELRRVT VLWDELWLGV LLQQHMYVLR RIQQLEDEVK 730 740 750 760 770 780 RVQNNNTLRK EEKIAIMREK HTALMKPIVF ALEHVRSITA APAETPHEKW FQDNYGDAIE 790 800 810 820 830 840 NALEKLKTPL NPAKPGSSWI PFKEIMLSLQ QRAQKRASYI LRLEEISPWL AAMTNTEIAL 850 860 870 880 890 900 PGEVSARDTV TIHSVGGTIT ILPTKTKPKK LLFLGSDGKS YPYLFKGLED LHLDERIMQF 910 920 930 940 950 960 LSIVNTMFAT INRQETPRFH ARHYSVTPLG TRSGLIQWVD GATPLFGLYK RWQQREAALQ 970 980 990 1000 1010 1020 AQKAQDSYQT PQNPGIVPRP SELYYSKIGP ALKTVGLSLD VSRRDWPLHV MKAVLEELME 1030 1040 1050 1060 1070 1080 ATPPNLLAKE LWSSCTTPDE WWRVTQSYAR STAVMSMVGY IIGLGDRHLD NVLIDMTTGE 1090 1100 1110 1120 1130 1140 VVHIDYNVCF EKGKSLRVPE KVPFRMTQNI ETALGVTGVE GVFRLSCEQV LHIMRRGRET 1150 1160 1170 1180 1190 1200 LLTLLEAFVY DPLVDWTAGG EAGFAGAVYG GGGQQAESKQ SKREMEREIT RSLFSSRVAE 1210 1220 1230 1240 1250 1260 IKVNWFKNRD EMLVVLPKLD GSLDEYLSLQ EQLTDVEKLQ GKLLEEIEFL EGAEGVDHPS 1270 1280 1290 1300 1310 1320 HTLQHRYSEH TQLQTQQRAV QEAIQVKLNE FEQWITHYQA AFNNLEATQL ASLLQEISTQ 1330 1340 1350 1360 1370 1380 MDLGPPSYVP ATAFLQNAGQ AHLISQCEQL EGEVGALLQQ RRSVLRGCLE QLHHYATVAL 1390 1400 1410 1420 1430 1440 QYPKAIFQKH RIEQWKTWME ELICNTTVER CQELYRKYEM QYAPQPPPTV CQFITATEMT 1450 1460 1470 1480 1490 1500 LQRYAADINS RLIRQVERLK QEAVTVPVCE DQLKEIERCI KVFLHENGEE GSLSLASVII 1510 1520 1530 1540 1550 1560 SALCTLTRRN LMMEGAASSA GEQLVDLTSR DGAWFLEELC SMSGNVTCLV QLLKQCHLVP 1570 1580 1590 1600 1610 1620 QDLDIPNPME ASETVHLANG VYTSLQELNS NFRQIIFPEA LRCLMKGEYT LESMLHELDG 1630 1640 1650 1660 1670 1680 LIEQTTDGVP LQTLVESLQA YLRNAAMGLE EETHAHYIDV ARLLHAQYGE LIQPRNGSVD 1690 1700 1710 1720 1730 1740 ETPKMSAGQM LLVAFDGMFA QVETAFSLLV EKLNKMEIPI AWRKIDIIRE ARSTQVNFFD 1750 1760 1770 1780 1790 1800 DDNHRQVLEE IFFLKRLQTI KEFFRLCGTF SKTLSGSSSL EDQNTVNGPV QIVNVKTLFR 1810 1820 1830 1840 1850 1860 NSCFSEDQMA KPIKAFTADF VRQLLIGLPN QALGLTLCSF ISALGVDIIA QVEAKDFGAE 1870 1880 1890 1900 1910 1920 SKVSVDDLCK KAVEHNIQIG KFSQLVMNRA TVLASSYDTA WKKHDLVRRL ETSISSCKTS 1930 1940 1950 1960 1970 1980 LQRVQLHIAM FQWQHEDLLI NRPQAMSVTP PPRSAILTSM KKKLHTLSQI ETSIATVQEK 1990 2000 2010 2020 2030 2040 LAALESSIEQ RLKWAGGANP ALAPVLQDFE ATIAERRNLV LKESQRASQV TFLCSNIIHF 2050 2060 2070 2080 2090 2100 ESLRTRTAEA LNLDAALFEL IKRCQQMCSF ASQFNSSVSE LELRLLQRVD TGLEHPIGSS 2110 2120 2130 2140 2150 2160 EWLLSAHKQL TQDMSTQRAI QTEKEQQIET VCETIQNLVD NIKTVLTGHN RQLGDVKHLL 2170 2180 2190 2200 2210 2220 KAMAKDEEAA LADGEDVPYE NSVRQFLGEY KSWQDNIQTV LFTLVQAMGQ VRSQEHVEML 2230 2240 2250 2260 2270 2280 QEITPTLKEL KTQSQSIYNN LVSFASPLVT DATNECSSPT SSATYQPSFA AAVRSNTGQK 2290 2300 2310 2320 2330 2340 TQPDVMSQNA RKLIQKNLAT SADTPPSTVP GTGKSVACSP KKAVRDPKTG KAVQERNSYA 2350 2360 2370 2380 2390 VSVWKRVKAK LEGRDVDPNR RMSVAEQVDY VIKEATNLDN LAQLYEGWTA WV

References

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[1]	"Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62." Sanchez P., De Carcer G., Sandoval I.V., Moscat J., Diaz-Meco M.T. Mol. Cell. Biol. 18:3069-3080(1998) [PubMed: 9566925] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[2]	"Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay." Yamashita A., Ohnishi T., Kashima I., Taya Y., Ohno S. Genes Dev. 15:2215-2228(2001) [PubMed: 11544179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION OF RENT1, ALTERNATIVE SPLICING, ENZYME REGULATION, PHOSPHORYLATION, INTERACTION WITH RENT1; UPF2 AND UPF3, MUTAGENESIS OF ASP-2331.
[3]	"Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein." Denning G., Jamieson L., Maquat L.E., Thompson E.A., Fields A.P. J. Biol. Chem. 276:22709-22714(2001) [PubMed: 11331269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, PHOSPHORYLATION OF RENT1, COFACTOR, ENZYME REGULATION, MUTAGENESIS OF ASP-2331.
[4]	"The mRNA surveillance protein hSMG-1 functions in genotoxic stress response pathways in mammalian cells." Brumbaugh K.M., Otterness D.M., Geisen C., Oliveira V., Brognard J., Li X., Lejeune F., Tibbetts R.S., Maquat L.E., Abraham R.T. Mol. Cell 14:585-598(2004) [PubMed: 15175154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, PHOSPHORYLATION OF TP53, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF ASP-2331, VARIANTS CYS-140 AND ILE-608.
[5]	"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro." Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:307-313(1997) [PubMed: 9455477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1670-3657. Tissue: Brain.
[6]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[7]	"Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo." Diaz-Meco M.T., Municio M.M., Sanchez P., Lozano J., Moscat J. Mol. Cell. Biol. 16:105-114(1996) [PubMed: 8524286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2874-3657, TISSUE SPECIFICITY, INTERACTION WITH PRKCI.
[8]	"Divergent origins and concerted expansion of two segmental duplications on chromosome 16." Eichler E.E., Johnson M.E., Alkan C., Tuzun E., Sahinalp C., Misceo D., Archidiacono N., Rocchi M. J. Hered. 92:462-468(2001) [PubMed: 11948212] [Abstract] Cited for: DUPLICATION.
[9]	"Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7." Ohnishi T., Yamashita A., Kashima I., Schell T., Anders K.R., Grimson A., Hachiya T., Hentze M.W., Anderson P., Ohno S. Mol. Cell 12:1187-1200(2003) [PubMed: 14636577] [Abstract] Cited for: INTERACTION WITH SMG5.
[10]	"Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1." Chiu S.-Y., Serin G., Ohara O., Maquat L.E. RNA 9:77-87(2003) [PubMed: 12554878] [Abstract] Cited for: INTERACTION WITH RENT1; UPF2; EST1A AND UPF3B.
[11]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3546 AND SER-3552, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3566 AND SER-3572, MASS SPECTROMETRY.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[14]	"SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay." Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S. Genes Dev. 23:1091-1105(2009) [PubMed: 19417104] [Abstract] Cited for: CATALYTIC ACTIVITY, IDENTIFICATION IN THE SMG1C COMPLEX.
[15]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-31; CYS-122; CYS-140; TYR-147; ASN-156; VAL-163; GLY-316; SER-461; ARG-542; SER-584; ILE-608; CYS-749; CYS-805; CYS-808; ILE-825; ASP-828; GLY-948; SER-965; LEU-1012; GLN-1025; SER-1068; HIS-1099; ARG-1271; PRO-1288; VAL-1328; PRO-1354; THR-1414; CYS-2167; SER-2254; LYS-2341; GLU-2726; SER-2885; ALA-2895; THR-3235 AND GLN-3579.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF186377 mRNA. Translation: AAK58892.1. AB061371 mRNA. Translation: BAB70696.1. AY014957 mRNA. Translation: AAK00511.1. AF395444 mRNA. Translation: AAM73708.1. AB007881 mRNA. Translation: BAA24851.2. U32581 mRNA. Translation: AAA86535.2. Different initiation.
IPI	IPI00183368. IPI00556369. IPI00639874. IPI00640955.
PIR	JC6084.
RefSeq	NP_055907.3.
UniGene	Hs.460179
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96Q15. 3 interactions.
PTM databases
PhosphoSite	Q96Q15.
Genome annotation databases
Ensembl	ENSG00000157106. Homo sapiens. [Contig view]
GeneID	23049.
KEGG	hsa:23049.
Organism-specific databases
GeneCards	GC16M018726.
H-InvDB	HIX0012849. HIX0012887. HIX0038531. HIX0038951. HIX0059633.
HGNC	HGNC:30045. SMG1.
HPA	HPA006870.
MIM	607032. gene.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96Q15.
HOVERGEN	Q96Q15.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Gene expression databases
ArrayExpress	Q96Q15.
Bgee	Q96Q15.
GermOnline	ENSG00000157106. Homo sapiens.
Family and domain databases
InterPro	IPR015519. Ataxia_Telang_Mut. IPR003152. FATC. IPR000357. HEAT. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR014009. PIK_FAT. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR11139:SF3. Ataxia_Telang_Mut. 1 hit.
Pfam	PF02260. FATC. 1 hit. PF02985. HEAT. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS50077. HEAT_REPEAT. False negative. PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44092.
SOURCE	Search...

Entry information

Entry name

SMG1_HUMAN

Accession

Primary (citable) accession number: Q96Q15
Secondary accession number(s): O43305 , Q13284, Q8NFX2, Q96QV0, Q96RW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)