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Q96Q11 (TRNT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CCA tRNA nucleotidyltransferase 1, mitochondrial

EC=2.7.7.72
Alternative name(s):
Mitochondrial tRNA nucleotidyl transferase, CCA-adding
mt CCA-adding enzyme
mt tRNA CCA-diphosphorylase
mt tRNA CCA-pyrophosphorylase
mt tRNA adenylyltransferase
Gene names
Name:TRNT1
ORF Names:CGI-47
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 1:Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. Ref.9

Isoform 2:Adds 2 C residues (CC-) to the 3' terminus of tRNA molecules instead of a complete CCA end as isoform 1 does (in vitro). Ref.9

Catalytic activity

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

Cofactor

Magnesium Probable.

Subunit structure

Monomer, and homodimer; disulfide-linked. Ref.14

Subcellular location

Mitochondrion Ref.8.

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Sequence caution

The sequence AAD34042.1 differs from that shown. Reason: Frameshift at position 16.

The sequence AAH12537.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96Q11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96Q11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-267: Missing.
Isoform 3 (identifier: Q96Q11-3)

The sequence of this isoform differs from the canonical sequence as follows:
     50-57: ELFVKENH → GINAFHEN
     58-434: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion Potential
Chain42 – 434393CCA tRNA nucleotidyltransferase 1, mitochondrial
PRO_0000004782

Sites

Active site771 By similarity
Active site791 By similarity
Active site1211 By similarity

Amino acid modifications

Modified residue4001Phosphoserine Ref.12
Modified residue4021N6-acetyllysine Ref.11
Disulfide bond373Interchain

Natural variations

Alternative sequence50 – 578ELFVKENH → GINAFHEN in isoform 3.
VSP_008440
Alternative sequence58 – 434377Missing in isoform 3.
VSP_008441
Alternative sequence248 – 26720Missing in isoform 2.
VSP_008442
Natural variant231P → L. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs334773 [ dbSNP | Ensembl ].
VAR_048698

Secondary structure

................................................................. 434
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 60B7387E203A53D0

FASTA43450,128
        10         20         30         40         50         60 
MLRCLYHWHR PVLNRRWSRL CLPKQYLFTM KLQSPEFQSL FTEGLKSLTE LFVKENHELR 

        70         80         90        100        110        120 
IAGGAVRDLL NGVKPQDIDF ATTATPTQMK EMFQSAGIRM INNRGEKHGT ITARLHEENF 

       130        140        150        160        170        180 
EITTLRIDVT TDGRHAEVEF TTDWQKDAER RDLTINSMFL GFDGTLFDYF NGYEDLKNKK 

       190        200        210        220        230        240 
VRFVGHAKQR IQEDYLRILR YFRFYGRIVD KPGDHDPETL EAIAENAKGL AGISGERIWV 

       250        260        270        280        290        300 
ELKKILVGNH VNHLIHLIYD LDVAPYIGLP ANASLEEFDK VSKNVDGFSP KPVTLLASLF 

       310        320        330        340        350        360 
KVQDDVTKLD LRLKIAKEEK NLGLFIVKNR KDLIKATDSS DPLKPYQDFI IDSREPDATT 

       370        380        390        400        410        420 
RVCELLKYQG EHCLLKEMQQ WSIPPFPVSG HDIRKVGISS GKEIGALLQQ LREQWKKSGY 

       430 
QMEKDELLSY IKKT 

« Hide

Isoform 2 [UniParc].

Checksum: 8E95DCE9B13C3743
Show »

FASTA41447,829
Isoform 3 [UniParc].

Checksum: B76351899690B303
Show »

FASTA576,937

References

« Hide 'large scale' references
[1]"Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases."
Nagaike T., Suzuki T., Tomari Y., Takemoto-Hori C., Negayama F., Watanabe K., Ueda T.
J. Biol. Chem. 276:40041-40049(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-23.
[2]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-23.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-23.
Tissue: Umbilical cord blood.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-23.
Tissue: Brain.
[5]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-23.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT LEU-23.
Tissue: Bone marrow.
[8]"Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing."
Simpson J.C., Wellenreuther R., Poustka A., Pepperkok R., Wiemann S.
EMBO Rep. 1:287-292(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"A splice variant of the human CCA-adding enzyme with modified activity."
Lizano E., Schuster J., Muller M., Kelso J., Morl M.
J. Mol. Biol. 366:1258-1265(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION (ISOFORM 2).
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization."
Augustin M.A., Reichert A.S., Betat H., Huber R., Moerl M., Steegborn C.
J. Mol. Biol. 328:985-994(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 30-434, DIMERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB063105 mRNA. Translation: BAB70662.1.
AF151805 mRNA. Translation: AAD34042.1. Frameshift.
AK290411 mRNA. Translation: BAF83100.1.
AL834397 mRNA. Translation: CAD39059.1.
AC024060 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW63886.1.
BC005184 mRNA. Translation: AAH05184.1.
BC012537 mRNA. Translation: AAH12537.1. Different initiation.
CCDSCCDS2561.2. [Q96Q11-1]
RefSeqNP_886552.2. NM_182916.2.
XP_005265253.1. XM_005265196.1. [Q96Q11-1]
UniGeneHs.732725.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OU5X-ray3.40A/B30-434[»]
ProteinModelPortalQ96Q11.
SMRQ96Q11. Positions 30-383.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119284. 17 interactions.
IntActQ96Q11. 1 interaction.
MINTMINT-4725447.
STRING9606.ENSP00000251607.

PTM databases

PhosphoSiteQ96Q11.

Polymorphism databases

DMDM296452848.

Proteomic databases

MaxQBQ96Q11.
PaxDbQ96Q11.
PRIDEQ96Q11.

Protocols and materials databases

DNASU51095.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000251607; ENSP00000251607; ENSG00000072756. [Q96Q11-1]
ENST00000280591; ENSP00000280591; ENSG00000072756. [Q96Q11-2]
ENST00000339437; ENSP00000342985; ENSG00000072756. [Q96Q11-3]
ENST00000402675; ENSP00000385745; ENSG00000072756. [Q96Q11-3]
ENST00000420393; ENSP00000400394; ENSG00000072756. [Q96Q11-3]
ENST00000434583; ENSP00000415100; ENSG00000072756. [Q96Q11-1]
GeneID51095.
KEGGhsa:51095.
UCSCuc003bpp.4. human. [Q96Q11-1]
uc010hbv.3. human. [Q96Q11-2]

Organism-specific databases

CTD51095.
GeneCardsGC03P003168.
HGNCHGNC:17341. TRNT1.
HPAHPA036938.
MIM612907. gene.
neXtProtNX_Q96Q11.
PharmGKBPA38446.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0617.
HOGENOMHOG000253345.
HOVERGENHBG061403.
InParanoidQ96Q11.
KOK00974.
OMAEPDATTR.
OrthoDBEOG7CCBR5.
PhylomeDBQ96Q11.
TreeFamTF313253.

Enzyme and pathway databases

BRENDA2.7.7.21. 2681.

Gene expression databases

ArrayExpressQ96Q11.
BgeeQ96Q11.
CleanExHS_TRNT1.
GenevestigatorQ96Q11.

Family and domain databases

InterProIPR002646. PolA_pol_head_dom.
[Graphical view]
PfamPF01743. PolyA_pol. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ96Q11.
GeneWikiTRNT1.
GenomeRNAi51095.
NextBio53789.
PROQ96Q11.
SOURCESearch...

Entry information

Entry nameTRNT1_HUMAN
AccessionPrimary (citable) accession number: Q96Q11
Secondary accession number(s): A8K2Z6 expand/collapse secondary AC list , B7WP13, C9JKA2, Q8ND57, Q9BS97, Q9Y362
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM