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Protein

Testis-specific serine/threonine-protein kinase 3

Gene

TSSK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-168, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATPPROSITE-ProRule annotation
Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ96PN8.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 3 (EC:2.7.11.1)
Short name:
TSK-3
Short name:
TSSK-3
Short name:
Testis-specific kinase 3
Alternative name(s):
Serine/threonine-protein kinase 22C
Gene namesi
Name:TSSK3
Synonyms:SPOGA3, STK22C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:15473. TSSK3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37964.

Polymorphism and mutation databases

BioMutaiTSSK3.
DMDMi30316270.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Testis-specific serine/threonine-protein kinase 3PRO_0000086770Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96PN8.
PRIDEiQ96PN8.

PTM databases

PhosphoSiteiQ96PN8.

Expressioni

Gene expression databases

BgeeiQ96PN8.
CleanExiHS_TSSK3.
ExpressionAtlasiQ96PN8. baseline and differential.
GenevisibleiQ96PN8. HS.

Organism-specific databases

HPAiHPA037515.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ADAMTSL4Q6UY14-33EBI-3918381,EBI-10173507
AESQ081173EBI-3918381,EBI-717810
EFHC2Q5JST63EBI-3918381,EBI-2349927
GOLGA2Q083793EBI-3918381,EBI-618309
IKZF3Q9UKT93EBI-3918381,EBI-747204
LNX1Q8TBB13EBI-3918381,EBI-739832
RBCK1Q9BYM83EBI-3918381,EBI-2340624
RELQ048643EBI-3918381,EBI-307352
TCF4P158843EBI-3918381,EBI-533224
TRIM9Q9C0263EBI-3918381,EBI-720828
VPS52Q8N1B43EBI-3918381,EBI-2799833
ZBTB25P242783EBI-3918381,EBI-739899

Protein-protein interaction databases

BioGridi123564. 18 interactions.
IntActiQ96PN8. 19 interactions.
STRINGi9606.ENSP00000362634.

Structurei

3D structure databases

ProteinModelPortaliQ96PN8.
SMRiQ96PN8. Positions 10-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 265256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ96PN8.
KOiK08811.
OMAiIIRVYEM.
PhylomeDBiQ96PN8.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96PN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKV IDKMGGPEEF
60 70 80 90 100
IQRFLPRELQ IVRTLDHKNI IQVYEMLESA DGKICLVMEL AEGGDVFDCV
110 120 130 140 150
LNGGPLPESR AKALFRQMVE AIRYCHGCGV AHRDLKCENA LLQGFNLKLT
160 170 180 190 200
DFGFAKVLPK SHRELSQTFC GSTAYAAPEV LQGIPHDSKK GDVWSMGVVL
210 220 230 240 250
YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLSISADCQD LLKRLLEPDM
260
ILRPSIEEVS WHPWLAST
Length:268
Mass (Da):30,102
Last modified:December 1, 2001 - v1
Checksum:iEF91F91DD216F671
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti71 – 711I → V.
Corresponds to variant rs35508255 [ dbSNP | Ensembl ].
VAR_051678
Natural varianti140 – 1401A → T.1 Publication
Corresponds to variant rs55786268 [ dbSNP | Ensembl ].
VAR_041245
Natural varianti235 – 2351S → L.1 Publication
Corresponds to variant rs35457991 [ dbSNP | Ensembl ].
VAR_041246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296450 mRNA. Translation: AAK97141.1.
AY048701 Transcribed RNA. Translation: AAL02128.1.
AL109945 Genomic DNA. Translation: CAI22888.1.
CH471059 Genomic DNA. Translation: EAX07532.1.
BC035354 mRNA. Translation: AAH35354.1.
CCDSiCCDS362.1.
RefSeqiNP_443073.1. NM_052841.3.
UniGeneiHs.512763.

Genome annotation databases

EnsembliENST00000373534; ENSP00000362634; ENSG00000162526.
GeneIDi81629.
KEGGihsa:81629.
UCSCiuc001bvf.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296450 mRNA. Translation: AAK97141.1.
AY048701 Transcribed RNA. Translation: AAL02128.1.
AL109945 Genomic DNA. Translation: CAI22888.1.
CH471059 Genomic DNA. Translation: EAX07532.1.
BC035354 mRNA. Translation: AAH35354.1.
CCDSiCCDS362.1.
RefSeqiNP_443073.1. NM_052841.3.
UniGeneiHs.512763.

3D structure databases

ProteinModelPortaliQ96PN8.
SMRiQ96PN8. Positions 10-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123564. 18 interactions.
IntActiQ96PN8. 19 interactions.
STRINGi9606.ENSP00000362634.

PTM databases

PhosphoSiteiQ96PN8.

Polymorphism and mutation databases

BioMutaiTSSK3.
DMDMi30316270.

Proteomic databases

PaxDbiQ96PN8.
PRIDEiQ96PN8.

Protocols and materials databases

DNASUi81629.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373534; ENSP00000362634; ENSG00000162526.
GeneIDi81629.
KEGGihsa:81629.
UCSCiuc001bvf.3. human.

Organism-specific databases

CTDi81629.
GeneCardsiGC01P032827.
H-InvDBHIX0000372.
HGNCiHGNC:15473. TSSK3.
HPAiHPA037515.
MIMi607660. gene.
neXtProtiNX_Q96PN8.
PharmGKBiPA37964.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ96PN8.
KOiK08811.
OMAiIIRVYEM.
PhylomeDBiQ96PN8.
TreeFamiTF352374.

Enzyme and pathway databases

SignaLinkiQ96PN8.

Miscellaneous databases

ChiTaRSiTSSK3. human.
GenomeRNAii81629.
NextBioi72014.
PROiQ96PN8.
SOURCEiSearch...

Gene expression databases

BgeeiQ96PN8.
CleanExiHS_TSSK3.
ExpressionAtlasiQ96PN8. baseline and differential.
GenevisibleiQ96PN8. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and chromosomal localization of a gene encoding a novel serine/threonine kinase belonging to the subfamily of testis-specific kinases."
    Visconti P.E., Hao Z., Purdon M.A., Stein P., Balsara B.R., Testa J.R., Herr J.C., Moss S.B., Kopf G.S.
    Genomics 77:163-170(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Cloning and characterization of a novel human TSSK3 gene."
    Mao Y., Xie Y., Jiang M.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal brain.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-140 AND LEU-235.

Entry informationi

Entry nameiTSSK3_HUMAN
AccessioniPrimary (citable) accession number: Q96PN8
Secondary accession number(s): Q5TEE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: July 22, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The mouse homolog is Stk22d.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.