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Protein

Zinc finger protein 385A

Gene

ZNF385A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that affects the localization and the translation of a subset of mRNA. May play a role in adipogenesis through binding to the 3'-UTR of CEBPA mRNA and regulation of its translation. Targets ITPR1 mRNA to dendrites in Purkinje cells, and may regulate its activity-dependent translation. With ELAVL1, binds the 3'-UTR of p53/TP53 mRNAs to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind CCNB1 mRNA. Alternatively, may also regulate p53/TP53 activity through direct protein-protein interaction. Interacts with p53/TP53 and promotes cell-cycle arrest over apoptosis enhancing preferentially the DNA binding and transactivation of p53/TP53 on cell-cycle arrest target genes over proapoptotic target genes. May also regulate the ubiquitination and stability of CDKN1A promoting DNA damage-induced cell cycle arrest. Also plays a role in megakaryocytes differentiation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri74 – 9825Matrin-type 1Add
BLAST
Zinc fingeri201 – 22525Matrin-type 2Add
BLAST
Zinc fingeri261 – 28525Matrin-type 3Add
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: UniProtKB
  • p53 binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-HSA-6804116. TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.
R-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.
R-HSA-69895. Transcriptional activation of cell cycle inhibitor p21.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 385A
Alternative name(s):
Hematopoietic zinc finger protein
Retinal zinc finger protein
Gene namesi
Name:ZNF385A
Synonyms:HZF, RZF, ZNF385
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:17521. ZNF385A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162410095.

Polymorphism and mutation databases

BioMutaiZNF385A.
DMDMi527504071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Zinc finger protein 385APRO_0000047554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei185 – 1851PhosphoserineCombined sources
Modified residuei248 – 2481PhosphothreonineCombined sources

Post-translational modificationi

Ubiquitinated upon prolonged exposure to genotoxic stress, which leads to proteasomal degradation of ZNF385A and releases p53/TP53 from cell-cycle arrest target gene promoters.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ96PM9.
PaxDbiQ96PM9.
PeptideAtlasiQ96PM9.
PRIDEiQ96PM9.

PTM databases

iPTMnetiQ96PM9.
PhosphoSiteiQ96PM9.

Expressioni

Tissue specificityi

Expressed predominantly in the retina.1 Publication

Inductioni

Up-regulated by p53/TP53 in response to DNA damage and oxidative stress.1 Publication

Gene expression databases

BgeeiENSG00000161642.
CleanExiHS_ZNF385A.
ExpressionAtlasiQ96PM9. baseline and differential.
GenevisibleiQ96PM9. HS.

Organism-specific databases

HPAiHPA039799.

Interactioni

Subunit structurei

Interacts with ELAVL1; the interaction is indirect, mRNA-dependent and may regulate p53/TP53 expression (By similarity). Interacts with p53/TP53; the interaction is direct and enhances p53/TP53 transactivation functions on cell-cycle arrest target genes, resulting in growth arrest.By similarity1 Publication

GO - Molecular functioni

  • p53 binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117442. 1 interaction.
IntActiQ96PM9. 2 interactions.
STRINGi9606.ENSP00000338927.

Structurei

3D structure databases

ProteinModelPortaliQ96PM9.
SMRiQ96PM9. Positions 254-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni145 – 351207Necessary for binding to ITPR1, CEBPA and p53/TP53 mRNAsBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 matrin-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri74 – 9825Matrin-type 1Add
BLAST
Zinc fingeri201 – 22525Matrin-type 2Add
BLAST
Zinc fingeri261 – 28525Matrin-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHAD. Eukaryota.
ENOG410Y1ZH. LUCA.
GeneTreeiENSGT00390000002371.
HOGENOMiHOG000231865.
HOVERGENiHBG054524.
InParanoidiQ96PM9.
OMAiRGREPGI.
OrthoDBiEOG091G08IG.
PhylomeDBiQ96PM9.
TreeFamiTF326622.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q96PM9-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILGSLSRAG PLPLLRQPPI MQPPLDLKQI LPFPLEPAPT LGLFSNYSTM
60 70 80 90 100
DPVQKAVLSH TFGGPLLKTK RPVISCNICQ IRFNSQSQAE AHYKGNRHAR
110 120 130 140 150
RVKGIEAAKT RGREPGVREP GDPAPPGSTP TNGDGVAPRP VSMENGLGPA
160 170 180 190 200
PGSPEKQPGS PSPPSIPETG QGVTKGEGGT PAPASLPGGS KEEEEKAKRL
210 220 230 240 250
LYCALCKVAV NSLSQLEAHN KGTKHKTILE ARSGLGPIKA YPRLGPPTPG
260 270 280 290 300
EPEAPAQDRT FHCEICNVKV NSEVQLKQHI SSRRHRDGVA GKPNPLLSRH
310 320 330 340 350
KKSRGAGELA GTLTFSKELP KSLAGGLLPS PLAVAAVMAA AAGSPLSLRP
360 370 380
APAAPLLQGP PITHPLLHPA PGPIRTAHGP ILFSPY
Length:386
Mass (Da):40,454
Last modified:July 24, 2013 - v2
Checksum:i21290C79D95CD620
GO
Isoform 1 (identifier: Q96PM9-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MILGSLSRAGPLPLLRQPPIM → M

Show »
Length:366
Mass (Da):38,342
Checksum:i8B77BAE4F921EFB6
GO
Isoform 2 (identifier: Q96PM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MILGSLSRAGPLPLLRQPPIM → M
     141-221: Missing.

Note: No experimental confirmation available.
Show »
Length:285
Mass (Da):30,180
Checksum:i75E46290CA710469
GO
Isoform 3 (identifier: Q96PM9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-221: Missing.

Show »
Length:305
Mass (Da):32,291
Checksum:iB92B9EB18DA73F19
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2121MILGS…QPPIM → M in isoform 1 and isoform 2. 4 PublicationsVSP_047448Add
BLAST
Alternative sequencei141 – 22181Missing in isoform 2 and isoform 3. 2 PublicationsVSP_047449Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461717 mRNA. Translation: AAS19275.1.
AF304052 mRNA. Translation: AAL08625.1.
AK024404 mRNA. Translation: BAB14910.1.
AK296564 mRNA. Translation: BAG59184.1.
AK315613 mRNA. Translation: BAG37982.1.
CR457327 mRNA. Translation: CAG33608.1.
AB593085 mRNA. Translation: BAJ84025.1.
AC078778 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96777.1.
BC029752 mRNA. Translation: AAH29752.1.
AL117462 mRNA. Translation: CAB55938.1.
CCDSiCCDS44910.1. [Q96PM9-3]
CCDS44911.1. [Q96PM9-4]
CCDS76563.1. [Q96PM9-2]
CCDS8879.1. [Q96PM9-1]
PIRiT17248.
RefSeqiNP_001124439.1. NM_001130967.2. [Q96PM9-4]
NP_001124440.1. NM_001130968.2. [Q96PM9-3]
NP_001276930.1. NM_001290001.1. [Q96PM9-2]
NP_001276931.1. NM_001290002.1. [Q96PM9-1]
NP_001276933.1. NM_001290004.1. [Q96PM9-1]
NP_056296.1. NM_015481.2. [Q96PM9-1]
XP_005268840.1. XM_005268783.4. [Q96PM9-1]
XP_006719405.1. XM_006719342.2. [Q96PM9-1]
UniGeneiHs.505653.

Genome annotation databases

EnsembliENST00000338010; ENSP00000338927; ENSG00000161642. [Q96PM9-4]
ENST00000352268; ENSP00000293385; ENSG00000161642. [Q96PM9-3]
ENST00000394313; ENSP00000377849; ENSG00000161642. [Q96PM9-1]
ENST00000546970; ENSP00000446913; ENSG00000161642. [Q96PM9-1]
ENST00000551109; ENSP00000449161; ENSG00000161642. [Q96PM9-1]
ENST00000551771; ENSP00000447162; ENSG00000161642. [Q96PM9-2]
GeneIDi25946.
KEGGihsa:25946.
UCSCiuc001sfw.2. human. [Q96PM9-4]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461717 mRNA. Translation: AAS19275.1.
AF304052 mRNA. Translation: AAL08625.1.
AK024404 mRNA. Translation: BAB14910.1.
AK296564 mRNA. Translation: BAG59184.1.
AK315613 mRNA. Translation: BAG37982.1.
CR457327 mRNA. Translation: CAG33608.1.
AB593085 mRNA. Translation: BAJ84025.1.
AC078778 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96777.1.
BC029752 mRNA. Translation: AAH29752.1.
AL117462 mRNA. Translation: CAB55938.1.
CCDSiCCDS44910.1. [Q96PM9-3]
CCDS44911.1. [Q96PM9-4]
CCDS76563.1. [Q96PM9-2]
CCDS8879.1. [Q96PM9-1]
PIRiT17248.
RefSeqiNP_001124439.1. NM_001130967.2. [Q96PM9-4]
NP_001124440.1. NM_001130968.2. [Q96PM9-3]
NP_001276930.1. NM_001290001.1. [Q96PM9-2]
NP_001276931.1. NM_001290002.1. [Q96PM9-1]
NP_001276933.1. NM_001290004.1. [Q96PM9-1]
NP_056296.1. NM_015481.2. [Q96PM9-1]
XP_005268840.1. XM_005268783.4. [Q96PM9-1]
XP_006719405.1. XM_006719342.2. [Q96PM9-1]
UniGeneiHs.505653.

3D structure databases

ProteinModelPortaliQ96PM9.
SMRiQ96PM9. Positions 254-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117442. 1 interaction.
IntActiQ96PM9. 2 interactions.
STRINGi9606.ENSP00000338927.

PTM databases

iPTMnetiQ96PM9.
PhosphoSiteiQ96PM9.

Polymorphism and mutation databases

BioMutaiZNF385A.
DMDMi527504071.

Proteomic databases

MaxQBiQ96PM9.
PaxDbiQ96PM9.
PeptideAtlasiQ96PM9.
PRIDEiQ96PM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338010; ENSP00000338927; ENSG00000161642. [Q96PM9-4]
ENST00000352268; ENSP00000293385; ENSG00000161642. [Q96PM9-3]
ENST00000394313; ENSP00000377849; ENSG00000161642. [Q96PM9-1]
ENST00000546970; ENSP00000446913; ENSG00000161642. [Q96PM9-1]
ENST00000551109; ENSP00000449161; ENSG00000161642. [Q96PM9-1]
ENST00000551771; ENSP00000447162; ENSG00000161642. [Q96PM9-2]
GeneIDi25946.
KEGGihsa:25946.
UCSCiuc001sfw.2. human. [Q96PM9-4]

Organism-specific databases

CTDi25946.
GeneCardsiZNF385A.
HGNCiHGNC:17521. ZNF385A.
HPAiHPA039799.
MIMi609124. gene.
neXtProtiNX_Q96PM9.
PharmGKBiPA162410095.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHAD. Eukaryota.
ENOG410Y1ZH. LUCA.
GeneTreeiENSGT00390000002371.
HOGENOMiHOG000231865.
HOVERGENiHBG054524.
InParanoidiQ96PM9.
OMAiRGREPGI.
OrthoDBiEOG091G08IG.
PhylomeDBiQ96PM9.
TreeFamiTF326622.

Enzyme and pathway databases

ReactomeiR-HSA-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-HSA-6804116. TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.
R-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.
R-HSA-69895. Transcriptional activation of cell cycle inhibitor p21.

Miscellaneous databases

ChiTaRSiZNF385A. human.
GenomeRNAii25946.
PROiQ96PM9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161642.
CleanExiHS_ZNF385A.
ExpressionAtlasiQ96PM9. baseline and differential.
GenevisibleiQ96PM9. HS.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZ385A_HUMAN
AccessioniPrimary (citable) accession number: Q96PM9
Secondary accession number(s): B2RDN5
, B4DKH2, F1T0F1, J3KNS3, Q5VH53, Q9H7R6, Q9UFU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 24, 2013
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.