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Protein

Zinc finger protein 385A

Gene

ZNF385A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein that affects the localization and the translation of a subset of mRNA. May play a role in adipogenesis through binding to the 3'-UTR of CEBPA mRNA and regulation of its translation. Targets ITPR1 mRNA to dendrites in Purkinje cells, and may regulate its activity-dependent translation. With ELAVL1, binds the 3'-UTR of p53/TP53 mRNAs to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind CCNB1 mRNA. Alternatively, may also regulate p53/TP53 activity through direct protein-protein interaction. Interacts with p53/TP53 and promotes cell-cycle arrest over apoptosis enhancing preferentially the DNA binding and transactivation of p53/TP53 on cell-cycle arrest target genes over proapoptotic target genes. May also regulate the ubiquitination and stability of CDKN1A promoting DNA damage-induced cell cycle arrest. Also plays a role in megakaryocytes differentiation.1 Publication

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 98Matrin-type 1Add BLAST25
Zinc fingeri201 – 225Matrin-type 2Add BLAST25
Zinc fingeri261 – 285Matrin-type 3Add BLAST25

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: UniProtKB
  • p53 binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processDNA damage, Transcription, Transcription regulation, Translation regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-HSA-6804116 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest
R-HSA-6804759 Regulation of TP53 Activity through Association with Co-factors
R-HSA-69895 Transcriptional activation of cell cycle inhibitor p21

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 385A
Alternative name(s):
Hematopoietic zinc finger protein
Retinal zinc finger protein
Gene namesi
Name:ZNF385A
Synonyms:HZF, RZF, ZNF385
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000161642.17
HGNCiHGNC:17521 ZNF385A
MIMi609124 gene
neXtProtiNX_Q96PM9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25946
OpenTargetsiENSG00000161642
PharmGKBiPA162410095

Polymorphism and mutation databases

BioMutaiZNF385A
DMDMi527504071

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475541 – 386Zinc finger protein 385AAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei185PhosphoserineCombined sources1
Modified residuei248PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated upon prolonged exposure to genotoxic stress, which leads to proteasomal degradation of ZNF385A and releases p53/TP53 from cell-cycle arrest target gene promoters.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96PM9
PaxDbiQ96PM9
PeptideAtlasiQ96PM9
PRIDEiQ96PM9

PTM databases

iPTMnetiQ96PM9
PhosphoSitePlusiQ96PM9

Expressioni

Tissue specificityi

Expressed predominantly in the retina.1 Publication

Inductioni

Up-regulated by p53/TP53 in response to DNA damage and oxidative stress.1 Publication

Gene expression databases

BgeeiENSG00000161642
CleanExiHS_ZNF385A
ExpressionAtlasiQ96PM9 baseline and differential
GenevisibleiQ96PM9 HS

Organism-specific databases

HPAiHPA039799

Interactioni

Subunit structurei

Interacts with ELAVL1; the interaction is indirect, mRNA-dependent and may regulate p53/TP53 expression (By similarity). Interacts with p53/TP53; the interaction is direct and enhances p53/TP53 transactivation functions on cell-cycle arrest target genes, resulting in growth arrest.By similarity1 Publication

GO - Molecular functioni

  • p53 binding Source: UniProtKB

Protein-protein interaction databases

BioGridi1174421 interactor.
IntActiQ96PM9 2 interactors.
STRINGi9606.ENSP00000338927

Structurei

3D structure databases

ProteinModelPortaliQ96PM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 351Necessary for binding to ITPR1, CEBPA and p53/TP53 mRNAsBy similarityAdd BLAST207

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 98Matrin-type 1Add BLAST25
Zinc fingeri201 – 225Matrin-type 2Add BLAST25
Zinc fingeri261 – 285Matrin-type 3Add BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHAD Eukaryota
ENOG410Y1ZH LUCA
GeneTreeiENSGT00390000002371
HOGENOMiHOG000231865
HOVERGENiHBG054524
InParanoidiQ96PM9
OMAiRRHRDGM
OrthoDBiEOG091G08IG
PhylomeDBiQ96PM9
TreeFamiTF326622

Family and domain databases

InterProiView protein in InterPro
IPR003604 Matrin/U1-like-C_Znf_C2H2
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SM00451 ZnF_U1, 3 hits
SUPFAMiSSF57667 SSF57667, 3 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q96PM9-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILGSLSRAG PLPLLRQPPI MQPPLDLKQI LPFPLEPAPT LGLFSNYSTM
60 70 80 90 100
DPVQKAVLSH TFGGPLLKTK RPVISCNICQ IRFNSQSQAE AHYKGNRHAR
110 120 130 140 150
RVKGIEAAKT RGREPGVREP GDPAPPGSTP TNGDGVAPRP VSMENGLGPA
160 170 180 190 200
PGSPEKQPGS PSPPSIPETG QGVTKGEGGT PAPASLPGGS KEEEEKAKRL
210 220 230 240 250
LYCALCKVAV NSLSQLEAHN KGTKHKTILE ARSGLGPIKA YPRLGPPTPG
260 270 280 290 300
EPEAPAQDRT FHCEICNVKV NSEVQLKQHI SSRRHRDGVA GKPNPLLSRH
310 320 330 340 350
KKSRGAGELA GTLTFSKELP KSLAGGLLPS PLAVAAVMAA AAGSPLSLRP
360 370 380
APAAPLLQGP PITHPLLHPA PGPIRTAHGP ILFSPY
Length:386
Mass (Da):40,454
Last modified:July 24, 2013 - v2
Checksum:i21290C79D95CD620
GO
Isoform 1 (identifier: Q96PM9-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MILGSLSRAGPLPLLRQPPIM → M

Show »
Length:366
Mass (Da):38,342
Checksum:i8B77BAE4F921EFB6
GO
Isoform 2 (identifier: Q96PM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MILGSLSRAGPLPLLRQPPIM → M
     141-221: Missing.

Note: No experimental confirmation available.
Show »
Length:285
Mass (Da):30,180
Checksum:i75E46290CA710469
GO
Isoform 3 (identifier: Q96PM9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-221: Missing.

Show »
Length:305
Mass (Da):32,291
Checksum:iB92B9EB18DA73F19
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0474481 – 21MILGS…QPPIM → M in isoform 1 and isoform 2. 4 PublicationsAdd BLAST21
Alternative sequenceiVSP_047449141 – 221Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461717 mRNA Translation: AAS19275.1
AF304052 mRNA Translation: AAL08625.1
AK024404 mRNA Translation: BAB14910.1
AK296564 mRNA Translation: BAG59184.1
AK315613 mRNA Translation: BAG37982.1
CR457327 mRNA Translation: CAG33608.1
AB593085 mRNA Translation: BAJ84025.1
AC078778 Genomic DNA No translation available.
AC079313 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96777.1
BC029752 mRNA Translation: AAH29752.1
AL117462 mRNA Translation: CAB55938.1
CCDSiCCDS44910.1 [Q96PM9-3]
CCDS44911.1 [Q96PM9-4]
CCDS76563.1 [Q96PM9-2]
CCDS8879.1 [Q96PM9-1]
PIRiT17248
RefSeqiNP_001124439.1, NM_001130967.2 [Q96PM9-4]
NP_001124440.1, NM_001130968.2 [Q96PM9-3]
NP_001276930.1, NM_001290001.1 [Q96PM9-2]
NP_001276931.1, NM_001290002.1 [Q96PM9-1]
NP_001276933.1, NM_001290004.1 [Q96PM9-1]
NP_056296.1, NM_015481.2 [Q96PM9-1]
XP_005268840.1, XM_005268783.4 [Q96PM9-1]
XP_006719405.1, XM_006719342.2 [Q96PM9-1]
XP_016874663.1, XM_017019174.1 [Q96PM9-1]
UniGeneiHs.505653

Genome annotation databases

EnsembliENST00000338010; ENSP00000338927; ENSG00000161642 [Q96PM9-4]
ENST00000352268; ENSP00000293385; ENSG00000161642 [Q96PM9-3]
ENST00000394313; ENSP00000377849; ENSG00000161642 [Q96PM9-1]
ENST00000546970; ENSP00000446913; ENSG00000161642 [Q96PM9-1]
ENST00000551109; ENSP00000449161; ENSG00000161642 [Q96PM9-1]
ENST00000551771; ENSP00000447162; ENSG00000161642 [Q96PM9-2]
GeneIDi25946
KEGGihsa:25946
UCSCiuc001sfw.2 human [Q96PM9-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZ385A_HUMAN
AccessioniPrimary (citable) accession number: Q96PM9
Secondary accession number(s): B2RDN5
, B4DKH2, F1T0F1, J3KNS3, Q5VH53, Q9H7R6, Q9UFU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 24, 2013
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome