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Protein

RNA-binding protein 14

Gene

RBM14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835).2 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • protein binding, bridging Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB

GO - Biological processi

  • centriole assembly Source: UniProtKB
  • DNA recombination Source: UniProtKB
  • DNA repair Source: UniProtKB
  • DNA replication Source: UniProtKB
  • glucocorticoid receptor signaling pathway Source: UniProtKB
  • histone deacetylation Source: UniProtKB
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • negative regulation of centriole replication Source: CACAO
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • response to hormone Source: UniProtKB
  • SMAD protein signal transduction Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 14
Alternative name(s):
Paraspeckle protein 2
Short name:
PSP2
RNA-binding motif protein 14
RRM-containing coactivator activator/modulator
Synaptotagmin-interacting protein
Short name:
SYT-interacting protein
Gene namesi
Name:RBM14
Synonyms:SIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14219. RBM14.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • mediator complex Source: UniProtKB
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10432.
OpenTargetsiENSG00000239306.
ENSG00000248643.
PharmGKBiPA34263.

Polymorphism and mutation databases

BioMutaiRBM14.
DMDMi73913750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817741 – 669RNA-binding protein 14Add BLAST669

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei161PhosphoserineCombined sources1
Modified residuei164N6-acetyllysineBy similarity1
Modified residuei206PhosphothreonineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei244PhosphoserineCombined sources1
Modified residuei256PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Modified residuei572PhosphothreonineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1
Modified residuei620PhosphoserineCombined sources1
Modified residuei623PhosphoserineCombined sources1
Modified residuei627PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1
Modified residuei649PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96PK6.
MaxQBiQ96PK6.
PaxDbiQ96PK6.
PeptideAtlasiQ96PK6.
PRIDEiQ96PK6.

PTM databases

iPTMnetiQ96PK6.
PhosphoSitePlusiQ96PK6.
SwissPalmiQ96PK6.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Gene expression databases

BgeeiENSG00000239306.
ExpressionAtlasiQ96PK6. baseline and differential.
GenevisibleiQ96PK6. HS.

Organism-specific databases

HPAiHPA006628.
HPA047849.

Interactioni

Subunit structurei

Isoform 1: Interacts with NCOA6, CITED1 and XRCC5/KU86 (PubMed:11443112). Isoform 1: Interacts with SS18 isoform 1 (PubMed:15919756). Isoform 1: Interacts with SS18 isoform 2 (PubMed:15919756). Interacts with STIL and interferes with its interaction with CENPJ. Interacts with gamma-tubulin (PubMed:25385835).3 Publications

GO - Molecular functioni

  • protein binding, bridging Source: UniProtKB

Protein-protein interaction databases

BioGridi115700. 112 interactors.
1529298. 29 interactors.
DIPiDIP-50126N.
IntActiQ96PK6. 38 interactors.
MINTiMINT-2798129.
STRINGi9606.ENSP00000311747.

Structurei

Secondary structure

1669
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi81 – 85Combined sources5
Helixi92 – 102Combined sources11
Beta strandi105 – 110Combined sources6
Beta strandi115 – 120Combined sources6
Helixi122 – 132Combined sources11
Beta strandi143 – 147Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNPNMR-A77-153[»]
ProteinModelPortaliQ96PK6.
SMRiQ96PK6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96PK6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 73RRM 1PROSITE-ProRule annotationAdd BLAST73
Domaini79 – 149RRM 2PROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 354TRBP-interacting domain; interaction with STIL1 PublicationAdd BLAST48

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi224 – 569Ala-richAdd BLAST346

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IES1. Eukaryota.
ENOG4111RA3. LUCA.
GeneTreeiENSGT00390000020883.
HOGENOMiHOG000026790.
HOVERGENiHBG053180.
InParanoidiQ96PK6.
KOiK13189.
OMAiYFGRDRS.
OrthoDBiEOG091G05UU.
PhylomeDBiQ96PK6.
TreeFamiTF320661.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96PK6-1) [UniParc]FASTAAdd to basket
Also known as: CoAA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIFVGNVDG ADTTPEELAA LFAPYGTVMS CAVMKQFAFV HMRENAGALR
60 70 80 90 100
AIEALHGHEL RPGRALVVEM SRPRPLNTWK IFVGNVSAAC TSQELRSLFE
110 120 130 140 150
RRGRVIECDV VKDYAFVHME KEADAKAAIA QLNGKEVKGK RINVELSTKG
160 170 180 190 200
QKKGPGLAVQ SGDKTKKPGA GDTAFPGTGG FSATFDYQQA FGNSTGGFDG
210 220 230 240 250
QARQPTPPFF GRDRSPLRRS PPRASYVAPL TAQPATYRAQ PSVSLGAAYR
260 270 280 290 300
AQPSASLGVG YRTQPMTAQA ASYRAQPSVS LGAPYRGQLA SPSSQSAAAS
310 320 330 340 350
SLGPYGGAQP SASALSSYGG QAAAASSLNS YGAQGSSLAS YGNQPSSYGA
360 370 380 390 400
QAASSYGVRA AASSYNTQGA ASSLGSYGAQ AASYGAQSAA SSLAYGAQAA
410 420 430 440 450
SYNAQPSASY NAQSAPYAAQ QAASYSSQPA AYVAQPATAA AYASQPAAYA
460 470 480 490 500
AQATTPMAGS YGAQPVVQTQ LNSYGAQASM GLSGSYGAQS AAAATGSYGA
510 520 530 540 550
AAAYGAQPSA TLAAPYRTQS SASLAASYAA QQHPQAAASY RGQPGNAYDG
560 570 580 590 600
AGQPSAAYLS MSQGAVANAN STPPPYERTR LSPPRASYDD PYKKAVAMSK
610 620 630 640 650
RYGSDRRLAE LSDYRRLSES QLSFRRSPTK SSLDYRRLPD AHSDYARYSG
660
SYNDYLRAAQ MHSGYQRRM
Length:669
Mass (Da):69,492
Last modified:August 30, 2005 - v2
Checksum:i565C5EF51B6881FD
GO
Isoform 2 (identifier: Q96PK6-2) [UniParc]FASTAAdd to basket
Also known as: CoAM

The sequence of this isoform differs from the canonical sequence as follows:
     151-156: QKKGPG → MVPTGV
     157-669: Missing.

Show »
Length:156
Mass (Da):17,117
Checksum:iBCD4C7DA01982D64
GO
Isoform 3 (identifier: Q96PK6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-118: DYAFVH → GGMCVG
     119-669: Missing.

Note: No experimental confirmation available.
Show »
Length:118
Mass (Da):12,894
Checksum:iCDC3102FDFAF75A5
GO
Isoform 4 (identifier: Q96PK6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-119: DYAFVHM → GMVPTGV
     120-669: Missing.

Show »
Length:119
Mass (Da):13,031
Checksum:i9B1DC069EB814F75
GO
Isoform 5 (identifier: Q96PK6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-339: KDYAFVHMEK...SYGAQGSSLA → KGKRMHVQLS...YADRARYSAF
     340-669: Missing.

Show »
Length:339
Mass (Da):37,035
Checksum:i3618E2DA670C7E21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti560S → T in AAK77961 (PubMed:11443112).Curated1
Sequence conflicti609A → V in BAD96550 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047494112 – 339KDYAF…GSSLA → KGKRMHVQLSTSRLRTAPGM GDQSGCYRCGKEGHWSKECP IDRSGRVADLTEQYNEQYGA VRTPYTMSYGDSLYYNNAYG ALDAYYKRCRAARSYEAVAA AAASVYNYAEQTLSQLPQVQ NTAMASHLTSTSLDPYDRHL LPTSGAAATAAAAAAAAAAV TAASTSYYGRDRSPLRRATA PVPTVGEGYGYGHESELSQA SAAARNSLYDMARYEREQYA DRARYSAF in isoform 5. 1 PublicationAdd BLAST228
Alternative sequenceiVSP_047109113 – 119DYAFVHM → GMVPTGV in isoform 4. Curated7
Alternative sequenceiVSP_044641113 – 118DYAFVH → GGMCVG in isoform 3. 2 Publications6
Alternative sequenceiVSP_044642119 – 669Missing in isoform 3. 2 PublicationsAdd BLAST551
Alternative sequenceiVSP_047110120 – 669Missing in isoform 4. CuratedAdd BLAST550
Alternative sequenceiVSP_015078151 – 156QKKGPG → MVPTGV in isoform 2. 1 Publication6
Alternative sequenceiVSP_015079157 – 669Missing in isoform 2. 1 PublicationAdd BLAST513
Alternative sequenceiVSP_047495340 – 669Missing in isoform 5. 1 PublicationAdd BLAST330

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315632 mRNA. Translation: AAK77961.1.
AF315633 mRNA. Translation: AAK77962.1.
DQ294957 mRNA. Translation: ABB99396.1.
EU287938 mRNA. Translation: ABY74511.1.
AF080561 mRNA. Translation: AAC64058.1.
AK021768 mRNA. Translation: BAG51046.1.
AK222830 mRNA. Translation: BAD96550.1.
AP001157 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74552.1.
BC000488 mRNA. Translation: AAH00488.1.
BE885635 mRNA. No translation available.
CR749306 mRNA. Translation: CAH18161.1.
CCDSiCCDS55772.1. [Q96PK6-2]
CCDS55773.1. [Q96PK6-4]
CCDS8147.1. [Q96PK6-1]
RefSeqiNP_001185765.1. NM_001198836.1. [Q96PK6-2]
NP_001185766.1. NM_001198837.1. [Q96PK6-4]
NP_001185774.1. NM_001198845.1. [Q96PK6-5]
NP_006319.1. NM_006328.3. [Q96PK6-1]
UniGeneiHs.523822.
Hs.533712.

Genome annotation databases

EnsembliENST00000310137; ENSP00000311747; ENSG00000239306. [Q96PK6-1]
ENST00000393979; ENSP00000377548; ENSG00000239306. [Q96PK6-2]
ENST00000409738; ENSP00000386995; ENSG00000239306. [Q96PK6-4]
GeneIDi100526737.
10432.
KEGGihsa:100526737.
hsa:10432.
UCSCiuc001oit.4. human. [Q96PK6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315632 mRNA. Translation: AAK77961.1.
AF315633 mRNA. Translation: AAK77962.1.
DQ294957 mRNA. Translation: ABB99396.1.
EU287938 mRNA. Translation: ABY74511.1.
AF080561 mRNA. Translation: AAC64058.1.
AK021768 mRNA. Translation: BAG51046.1.
AK222830 mRNA. Translation: BAD96550.1.
AP001157 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74552.1.
BC000488 mRNA. Translation: AAH00488.1.
BE885635 mRNA. No translation available.
CR749306 mRNA. Translation: CAH18161.1.
CCDSiCCDS55772.1. [Q96PK6-2]
CCDS55773.1. [Q96PK6-4]
CCDS8147.1. [Q96PK6-1]
RefSeqiNP_001185765.1. NM_001198836.1. [Q96PK6-2]
NP_001185766.1. NM_001198837.1. [Q96PK6-4]
NP_001185774.1. NM_001198845.1. [Q96PK6-5]
NP_006319.1. NM_006328.3. [Q96PK6-1]
UniGeneiHs.523822.
Hs.533712.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNPNMR-A77-153[»]
ProteinModelPortaliQ96PK6.
SMRiQ96PK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115700. 112 interactors.
1529298. 29 interactors.
DIPiDIP-50126N.
IntActiQ96PK6. 38 interactors.
MINTiMINT-2798129.
STRINGi9606.ENSP00000311747.

PTM databases

iPTMnetiQ96PK6.
PhosphoSitePlusiQ96PK6.
SwissPalmiQ96PK6.

Polymorphism and mutation databases

BioMutaiRBM14.
DMDMi73913750.

Proteomic databases

EPDiQ96PK6.
MaxQBiQ96PK6.
PaxDbiQ96PK6.
PeptideAtlasiQ96PK6.
PRIDEiQ96PK6.

Protocols and materials databases

DNASUi10432.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310137; ENSP00000311747; ENSG00000239306. [Q96PK6-1]
ENST00000393979; ENSP00000377548; ENSG00000239306. [Q96PK6-2]
ENST00000409738; ENSP00000386995; ENSG00000239306. [Q96PK6-4]
GeneIDi100526737.
10432.
KEGGihsa:100526737.
hsa:10432.
UCSCiuc001oit.4. human. [Q96PK6-1]

Organism-specific databases

CTDi100526737.
10432.
DisGeNETi10432.
GeneCardsiRBM14.
HGNCiHGNC:14219. RBM14.
HPAiHPA006628.
HPA047849.
MIMi612409. gene.
neXtProtiNX_Q96PK6.
OpenTargetsiENSG00000239306.
ENSG00000248643.
PharmGKBiPA34263.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IES1. Eukaryota.
ENOG4111RA3. LUCA.
GeneTreeiENSGT00390000020883.
HOGENOMiHOG000026790.
HOVERGENiHBG053180.
InParanoidiQ96PK6.
KOiK13189.
OMAiYFGRDRS.
OrthoDBiEOG091G05UU.
PhylomeDBiQ96PK6.
TreeFamiTF320661.

Miscellaneous databases

ChiTaRSiRBM14. human.
EvolutionaryTraceiQ96PK6.
GeneWikiiRBM14.
PROiQ96PK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000239306.
ExpressionAtlasiQ96PK6. baseline and differential.
GenevisibleiQ96PK6. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM14_HUMAN
AccessioniPrimary (citable) accession number: Q96PK6
Secondary accession number(s): B0LM41
, B3KMN4, D6RGD8, O75932, Q2PYN1, Q53GV1, Q68DQ9, Q96PK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.