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Protein

RNA-binding protein 14

Gene

RBM14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).3 Publications

Caution

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • protein binding, bridging Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB

GO - Biological processi

  • activation of innate immune response Source: UniProtKB
  • centriole assembly Source: UniProtKB
  • DNA recombination Source: UniProtKB
  • DNA repair Source: UniProtKB
  • DNA replication Source: UniProtKB
  • glucocorticoid receptor signaling pathway Source: UniProtKB
  • histone deacetylation Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • negative regulation of centriole replication Source: CACAO
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • response to hormone Source: UniProtKB
  • SMAD protein signal transduction Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-8941326 RUNX2 regulates bone development

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 14
Alternative name(s):
Paraspeckle protein 2
Short name:
PSP2
RNA-binding motif protein 14
RRM-containing coactivator activator/modulator
Synaptotagmin-interacting protein
Short name:
SYT-interacting protein
Gene namesi
Name:RBM14
Synonyms:SIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000239306.4
HGNCiHGNC:14219 RBM14
MIMi612409 gene
neXtProtiNX_Q96PK6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi100526737
10432
OpenTargetsiENSG00000239306
ENSG00000248643
PharmGKBiPA34263

Polymorphism and mutation databases

BioMutaiRBM14
DMDMi73913750

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817741 – 669RNA-binding protein 14Add BLAST669

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki138Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki153Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei161PhosphoserineCombined sources1
Modified residuei164N6-acetyllysine; alternateBy similarity1
Cross-linki164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei206PhosphothreonineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei244PhosphoserineCombined sources1
Modified residuei256PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Modified residuei572PhosphothreonineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Cross-linki600Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei618PhosphoserineCombined sources1
Modified residuei620PhosphoserineCombined sources1
Modified residuei623PhosphoserineCombined sources1
Modified residuei627PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1
Modified residuei649PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96PK6
MaxQBiQ96PK6
PaxDbiQ96PK6
PeptideAtlasiQ96PK6
PRIDEiQ96PK6

PTM databases

iPTMnetiQ96PK6
PhosphoSitePlusiQ96PK6
SwissPalmiQ96PK6

Expressioni

Tissue specificityi

Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Gene expression databases

BgeeiENSG00000239306
ExpressionAtlasiQ96PK6 baseline and differential
GenevisibleiQ96PK6 HS

Organism-specific databases

HPAiHPA006628
HPA047849

Interactioni

Subunit structurei

Isoform 1: Interacts with NCOA6, CITED1 and XRCC5/KU86 (PubMed:11443112). Isoform 1: Interacts with SS18 isoform 1 (PubMed:15919756). Isoform 1: Interacts with SS18 isoform 2 (PubMed:15919756). Interacts with STIL and interferes with its interaction with CENPJ. Interacts with gamma-tubulin (PubMed:25385835).Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein binding, bridging Source: UniProtKB

Protein-protein interaction databases

BioGridi115700124 interactors.
152929834 interactors.
DIPiDIP-50126N
IntActiQ96PK6 51 interactors.
MINTiQ96PK6
STRINGi9606.ENSP00000311747

Structurei

Secondary structure

1669
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi81 – 85Combined sources5
Helixi92 – 102Combined sources11
Beta strandi105 – 110Combined sources6
Beta strandi115 – 120Combined sources6
Helixi122 – 132Combined sources11
Beta strandi143 – 147Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNPNMR-A77-153[»]
ProteinModelPortaliQ96PK6
SMRiQ96PK6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96PK6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 73RRM 1PROSITE-ProRule annotationAdd BLAST73
Domaini79 – 149RRM 2PROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 354TRBP-interacting domain; interaction with STIL1 PublicationAdd BLAST48

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi224 – 569Ala-richAdd BLAST346

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IES1 Eukaryota
ENOG4111RA3 LUCA
GeneTreeiENSGT00390000020883
HOGENOMiHOG000026790
HOVERGENiHBG053180
InParanoidiQ96PK6
KOiK13189
OMAiIMSKRYG
OrthoDBiEOG091G05UU
PhylomeDBiQ96PK6
TreeFamiTF320661

Family and domain databases

CDDicd12608 RRM1_CoAA, 1 hit
cd12609 RRM2_CoAA, 1 hit
Gene3Di3.30.70.3302 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034506 RBM14_RRM1
IPR034507 RBM14_RRM2
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96PK6-1) [UniParc]FASTAAdd to basket
Also known as: CoAA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIFVGNVDG ADTTPEELAA LFAPYGTVMS CAVMKQFAFV HMRENAGALR
60 70 80 90 100
AIEALHGHEL RPGRALVVEM SRPRPLNTWK IFVGNVSAAC TSQELRSLFE
110 120 130 140 150
RRGRVIECDV VKDYAFVHME KEADAKAAIA QLNGKEVKGK RINVELSTKG
160 170 180 190 200
QKKGPGLAVQ SGDKTKKPGA GDTAFPGTGG FSATFDYQQA FGNSTGGFDG
210 220 230 240 250
QARQPTPPFF GRDRSPLRRS PPRASYVAPL TAQPATYRAQ PSVSLGAAYR
260 270 280 290 300
AQPSASLGVG YRTQPMTAQA ASYRAQPSVS LGAPYRGQLA SPSSQSAAAS
310 320 330 340 350
SLGPYGGAQP SASALSSYGG QAAAASSLNS YGAQGSSLAS YGNQPSSYGA
360 370 380 390 400
QAASSYGVRA AASSYNTQGA ASSLGSYGAQ AASYGAQSAA SSLAYGAQAA
410 420 430 440 450
SYNAQPSASY NAQSAPYAAQ QAASYSSQPA AYVAQPATAA AYASQPAAYA
460 470 480 490 500
AQATTPMAGS YGAQPVVQTQ LNSYGAQASM GLSGSYGAQS AAAATGSYGA
510 520 530 540 550
AAAYGAQPSA TLAAPYRTQS SASLAASYAA QQHPQAAASY RGQPGNAYDG
560 570 580 590 600
AGQPSAAYLS MSQGAVANAN STPPPYERTR LSPPRASYDD PYKKAVAMSK
610 620 630 640 650
RYGSDRRLAE LSDYRRLSES QLSFRRSPTK SSLDYRRLPD AHSDYARYSG
660
SYNDYLRAAQ MHSGYQRRM
Length:669
Mass (Da):69,492
Last modified:August 30, 2005 - v2
Checksum:i565C5EF51B6881FD
GO
Isoform 2 (identifier: Q96PK6-2) [UniParc]FASTAAdd to basket
Also known as: CoAM

The sequence of this isoform differs from the canonical sequence as follows:
     151-156: QKKGPG → MVPTGV
     157-669: Missing.

Show »
Length:156
Mass (Da):17,117
Checksum:iBCD4C7DA01982D64
GO
Isoform 3 (identifier: Q96PK6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-118: DYAFVH → GGMCVG
     119-669: Missing.

Note: No experimental confirmation available.
Show »
Length:118
Mass (Da):12,894
Checksum:iCDC3102FDFAF75A5
GO
Isoform 4 (identifier: Q96PK6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-119: DYAFVHM → GMVPTGV
     120-669: Missing.

Show »
Length:119
Mass (Da):13,031
Checksum:i9B1DC069EB814F75
GO
Isoform 5 (identifier: Q96PK6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-339: KDYAFVHMEK...SYGAQGSSLA → KGKRMHVQLS...YADRARYSAF
     340-669: Missing.

Show »
Length:339
Mass (Da):37,035
Checksum:i3618E2DA670C7E21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti560S → T in AAK77961 (PubMed:11443112).Curated1
Sequence conflicti609A → V in BAD96550 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047494112 – 339KDYAF…GSSLA → KGKRMHVQLSTSRLRTAPGM GDQSGCYRCGKEGHWSKECP IDRSGRVADLTEQYNEQYGA VRTPYTMSYGDSLYYNNAYG ALDAYYKRCRAARSYEAVAA AAASVYNYAEQTLSQLPQVQ NTAMASHLTSTSLDPYDRHL LPTSGAAATAAAAAAAAAAV TAASTSYYGRDRSPLRRATA PVPTVGEGYGYGHESELSQA SAAARNSLYDMARYEREQYA DRARYSAF in isoform 5. 1 PublicationAdd BLAST228
Alternative sequenceiVSP_047109113 – 119DYAFVHM → GMVPTGV in isoform 4. Curated7
Alternative sequenceiVSP_044641113 – 118DYAFVH → GGMCVG in isoform 3. 2 Publications6
Alternative sequenceiVSP_044642119 – 669Missing in isoform 3. 2 PublicationsAdd BLAST551
Alternative sequenceiVSP_047110120 – 669Missing in isoform 4. CuratedAdd BLAST550
Alternative sequenceiVSP_015078151 – 156QKKGPG → MVPTGV in isoform 2. 1 Publication6
Alternative sequenceiVSP_015079157 – 669Missing in isoform 2. 1 PublicationAdd BLAST513
Alternative sequenceiVSP_047495340 – 669Missing in isoform 5. 1 PublicationAdd BLAST330

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315632 mRNA Translation: AAK77961.1
AF315633 mRNA Translation: AAK77962.1
DQ294957 mRNA Translation: ABB99396.1
EU287938 mRNA Translation: ABY74511.1
AF080561 mRNA Translation: AAC64058.1
AK021768 mRNA Translation: BAG51046.1
AK222830 mRNA Translation: BAD96550.1
AP001157 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74552.1
BC000488 mRNA Translation: AAH00488.1
BE885635 mRNA No translation available.
CR749306 mRNA Translation: CAH18161.1
CCDSiCCDS55772.1 [Q96PK6-2]
CCDS55773.1 [Q96PK6-4]
CCDS8147.1 [Q96PK6-1]
RefSeqiNP_001185765.1, NM_001198836.1 [Q96PK6-2]
NP_001185766.1, NM_001198837.1 [Q96PK6-4]
NP_001185774.1, NM_001198845.1 [Q96PK6-5]
NP_006319.1, NM_006328.3 [Q96PK6-1]
UniGeneiHs.523822
Hs.533712

Genome annotation databases

EnsembliENST00000310137; ENSP00000311747; ENSG00000239306 [Q96PK6-1]
ENST00000393979; ENSP00000377548; ENSG00000239306 [Q96PK6-2]
ENST00000409738; ENSP00000386995; ENSG00000239306 [Q96PK6-4]
GeneIDi100526737
10432
KEGGihsa:100526737
hsa:10432
UCSCiuc001oit.4 human [Q96PK6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBM14_HUMAN
AccessioniPrimary (citable) accession number: Q96PK6
Secondary accession number(s): B0LM41
, B3KMN4, D6RGD8, O75932, Q2PYN1, Q53GV1, Q68DQ9, Q96PK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: April 25, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome