Q96PK2 (MACF4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-actin cross-linking factor 1, isoform 4

Gene names

Name:	MACF1
Synonyms:	ABP620, ACF7, KIAA0465, KIAA1251

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	5938 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in cross-linking cytoskeletal proteins by binding intermediate filaments to the N-terminal plectin repeats and microtubules to the C-terminus. Ref.1
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Tissue specificity	Expressed in heart, lung, pituitary and placenta, not found in brain, kidney, liver, pancreas or skeletal muscle. Ref.1
Sequence similarities	Belongs to the plakin or cytolinker family. UniProtKB Q9UPN3 Contains 2 EF-hand domains. Contains 1 GAR domain. Contains 19 plectin repeats. Contains 32 spectrin repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat
Ligand	Calcium
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle arrest Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 4 Ref.1 (identifier: Q96PK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 Ref.1 (identifier: Q9UPN3-1)

Also known as: Macf1b;

The sequence of this isoform can be found in the external entry Q9UPN3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 2 Ref.1 (identifier: Q9UPN3-2)

Also known as: Mac1fa;

The sequence of this isoform can be found in the external entry Q9UPN3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 3 Ref.1 (identifier: Q9UPN3-3)

The sequence of this isoform can be found in the external entry Q9UPN3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 5 (identifier: Q9UPN3-4)

The sequence of this isoform can be found in the external entry Q9UPN3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 5938	5938	Microtubule-actin cross-linking factor 1, isoform 4		PRO_0000073450
Regions
	Repeat	12 – 49	38	Plectin 1
	Repeat	53 – 88	36	Plectin 2
	Repeat	89 – 126	38	Plectin 3
	Repeat	130 – 164	35	Plectin 4
	Repeat	166 – 202	37	Plectin 5
	Repeat	203 – 240	38	Plectin 6
	Repeat	243 – 278	36	Plectin 7
	Repeat	279 – 316	38	Plectin 8
	Repeat	318 – 354	37	Plectin 9
	Repeat	725 – 762	38	Plectin 10
	Repeat	763 – 800	38	Plectin 11
	Repeat	801 – 838	38	Plectin 12
	Repeat	839 – 876	38	Plectin 13
	Repeat	897 – 934	38	Plectin 14
	Repeat	935 – 972	38	Plectin 15
	Repeat	975 – 1011	37	Plectin 16
	Repeat	1012 – 1049	38	Plectin 17
	Repeat	1121 – 1158	38	Plectin 18
	Repeat	1159 – 1196	38	Plectin 19
	Repeat	1925 – 2032	108	Spectrin 1
	Repeat	2052 – 2160	109	Spectrin 2
	Repeat	2211 – 2313	103	Spectrin 3
	Repeat	2320 – 2430	111	Spectrin 4
	Repeat	2437 – 2543	107	Spectrin 5
	Repeat	2550 – 2652	103	Spectrin 6
	Repeat	2659 – 2758	100	Spectrin 7
	Repeat	2765 – 2896	132	Spectrin 8
	Repeat	2903 – 3008	106	Spectrin 9
	Repeat	3015 – 3119	105	Spectrin 10
	Repeat	3126 – 3229	104	Spectrin 11
	Repeat	3236 – 3339	104	Spectrin 12
	Repeat	3346 – 3446	101	Spectrin 13
	Repeat	3453 – 3555	103	Spectrin 14
	Repeat	3562 – 3664	103	Spectrin 15
	Repeat	3671 – 3775	105	Spectrin 16
	Repeat	3782 – 3884	103	Spectrin 17
	Repeat	3891 – 3993	103	Spectrin 18
	Repeat	4000 – 4102	103	Spectrin 19
	Repeat	4109 – 4211	103	Spectrin 20
	Repeat	4218 – 4320	103	Spectrin 21
	Repeat	4327 – 4428	102	Spectrin 22
	Repeat	4438 – 4544	107	Spectrin 23
	Repeat	4551 – 4653	103	Spectrin 24
	Repeat	4660 – 4763	104	Spectrin 25
	Repeat	4770 – 4872	103	Spectrin 26
	Repeat	4879 – 4982	104	Spectrin 27
	Repeat	4989 – 5091	103	Spectrin 28
	Repeat	5098 – 5201	104	Spectrin 29
	Repeat	5208 – 5309	102	Spectrin 30
	Repeat	5316 – 5418	103	Spectrin 31
	Repeat	5425 – 5555	131	Spectrin 32
	Domain	5585 – 5620	36	EF-hand 1
	Domain	5621 – 5656	36	EF-hand 2
	Domain	5661 – 5739	79	GAR
	Calcium binding	5598 – 5610	13	1 Potential
	Calcium binding	5634 – 5646	13	2 Potential
	Coiled coil	1830 – 1936	107	Potential
	Coiled coil	2001 – 2192	192	Potential
	Coiled coil	2282 – 2345	64	Potential
	Coiled coil	2477 – 2507	31	Potential
	Coiled coil	2541 – 2654	114	Potential
	Coiled coil	2686 – 2814	129	Potential
	Coiled coil	2887 – 2919	33	Potential
	Coiled coil	3046 – 3197	152	Potential
	Coiled coil	3262 – 3503	242	Potential
	Coiled coil	3632 – 3666	35	Potential
	Coiled coil	3746 – 3779	34	Potential
	Coiled coil	3920 – 3984	65	Potential
	Coiled coil	4098 – 4168	71	Potential
	Coiled coil	4288 – 4308	21	Potential
	Coiled coil	4354 – 4386	33	Potential
	Coiled coil	4397 – 4433	37	Potential
	Coiled coil	4469 – 4489	21	Potential
	Coiled coil	4586 – 4720	135	Potential
	Coiled coil	4845 – 4880	36	Potential
	Coiled coil	4910 – 4939	30	Potential
	Coiled coil	4970 – 5000	31	Potential
	Coiled coil	5409 – 5437	29	Potential
	Coiled coil	5546 – 5569	24	Potential
Amino acid modifications
	Modified residue	1554	1	Phosphotyrosine Ref.5
	Modified residue	2362	1	Phosphoserine Ref.7
	Modified residue	2576	1	Phosphoserine Ref.6
	Modified residue	2577	1	Phosphoserine Ref.6
	Modified residue	2579	1	Phosphoserine Ref.6
	Modified residue	2930	1	Phosphoserine Ref.7
	Modified residue	2931	1	Phosphoserine Ref.7
	Modified residue	2956	1	Phosphoserine Ref.7
	Modified residue	4754	1	N6-acetyllysine Ref.8
	Modified residue	5627	1	Phosphothreonine Ref.3
	Modified residue	5797	1	Phosphoserine Ref.7
	Modified residue	5801	1	Phosphoserine Ref.7
	Modified residue	5804	1	Phosphothreonine Ref.7
	Modified residue	5823	1	Phosphothreonine Ref.4
	Modified residue	5842	1	Phosphoserine Ref.7
Natural variations
	Natural variant	39	1	I → V. Corresponds to variant rs16826069 [ dbSNP | Ensembl ].		VAR_055623
	Natural variant	498	1	T → I. Corresponds to variant rs16826072 [ dbSNP | Ensembl ].		VAR_055624
	Natural variant	916	1	A → G. Corresponds to variant rs10047195 [ dbSNP | Ensembl ].		VAR_055625
	Natural variant	1625	1	K → N. Corresponds to variant rs41270807 [ dbSNP | Ensembl ].		VAR_061083
	Natural variant	1743	1	K → T. Corresponds to variant rs17570718 [ dbSNP | Ensembl ].		VAR_055626
	Natural variant	2792	1	M → V. Corresponds to variant rs2296172 [ dbSNP | Ensembl ].		VAR_055627
	Natural variant	4745	1	K → R. Corresponds to variant rs682351 [ dbSNP | Ensembl ].		VAR_055628
	Natural variant	4852	1	A → T. Corresponds to variant rs587404 [ dbSNP | Ensembl ].		VAR_055629
	Natural variant	5172	1	S → T. Ref.1 Corresponds to variant rs668556 [ dbSNP | Ensembl ].		VAR_063125
	Natural variant	5296	1	T → I. Corresponds to variant rs2296174 [ dbSNP | Ensembl ].		VAR_055630
	Natural variant	5399	1	I → V. Corresponds to variant rs12068423 [ dbSNP | Ensembl ].		VAR_055631
Experimental info
	Sequence conflict	1712	1	P → S in AAL38997. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 4 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 360611C48946A058	FASTA	5,938	670,137
	10 20 30 40 50 60 MQKGLLDQDT GLVLLESQVI MSGLIAPETG ENLSLEEGIA RNLINPQMYQ QLRELQDALA 70 80 90 100 110 120 LISRLTESRG PLSVVEAIEK RIISETVGLK ILEAHLATGG FSLSPSENCI NLEEAFHQGL 130 140 150 160 170 180 ISAWLHSVLE SYLRTSKNLI DPNTAEKIGL LDLMQRCIVH QESGFKLLPV KQLAGGMVSL 190 200 210 220 230 240 KSGRKVSIFR AVQEGLIDRQ VTVRLLEAQL FAGGIVDPRT GHRLTVEEAV RHNLIDQDMA 250 260 270 280 290 300 CAILIRQLQT GGIIDTVTGQ RLTIDEAVSN DLVAAKIALV ILESLWSFMG LLWPESGEIL 310 320 330 340 350 360 PITDALEQGI VSTELAHKIL SNRQHIKALF LPATTEILSW KKAIESGILD RDLANNLKSI 370 380 390 400 410 420 CIPDVMPHMQ LADSAEQNIN PGAAVLPCSK SHPKATASQS ENLLFQLMTH SYINVQNGQR 430 440 450 460 470 480 LLLLDKELME TLTSRDEYQT SPPKVVEIGH QRQKTPEGLQ ESANVKISGT FSSGWTVRLP 490 500 510 520 530 540 EFQFSSQNKE YPDREDCTTE KGKKTTVETE DSSVENPEQD LFVEQKERNP NIDALKVINK 550 560 570 580 590 600 VKLEVQRQLI GTQREDQTAV SVRENASRGH LLTIPPAEAE GVPLVVDKDV FSVETPKKEH 610 620 630 640 650 660 QPLRNTSFTC QNEQAHTLET EYIHDETGGS HIKPQSKKLQ VQVKKTLGIK LELKSETDGN 670 680 690 700 710 720 VHPLDKKEML KKTFLAKDDH KESQEAQNIA GGSMMMSEKT DEEDSGREIF LSCSHPLELL 730 740 750 760 770 780 EEATLNVLSA QLLDGGIFHE QTGQKLLLNE AISRGIVPSH TAVKLMEKLN MFQGFFDSQT 790 800 810 820 830 840 CESLTTEEVI NEGLMDEKLL HNVLMADKAI SGVLDPRTQT LCSVKDAVTV GLLDKETATR 850 860 870 880 890 900 ILERQVVTGG IIDLKRGKKV SVTLASTLGL VDVADQPELI NLEKASKGRD AEKTVRERLI 910 920 930 940 950 960 SLQMETTGLI DPDSKAPLTV VQSIDRGLLE REEAVRLLTK QVVDGGIIHH ISGMRLSVDN 970 980 990 1000 1010 1020 AFRHGLIGED LAEKLKRVEN LNIHQIFNPE TKENISLPKA IKLDLITSDL KREIQEVQAF 1030 1040 1050 1060 1070 1080 TGNFVDLISG QRLTLAEAKK EGLLTNEAVL SPGMMHGIVD PENCRIVPYS ELVKKCKIDI 1090 1100 1110 1120 1130 1140 ESGQRYLEVI PFSDIKDGVS DKVLTLSQAI QLGKVDFAST LKVLEAQANT GGIIDTATGK 1150 1160 1170 1180 1190 1200 RLTLASALEE KLVDENMVRI IASHQVLNGG IVDIFSDQRV TLVEAIEKRL ISPELANMIQ 1210 1220 1230 1240 1250 1260 IDSSEFSDHR AQIEKQEGIE VCALQNEFLG KDMLIACNQT AEMSCNKVEE SERLFQVENQ 1270 1280 1290 1300 1310 1320 SAQEKVKVRV SDGEQAKKSR EISLKEFGCK DQRKPRMSSD AKEFISIINP HNLKGKSLGQ 1330 1340 1350 1360 1370 1380 VSLTHPYSEC DFKLKEVARN NMGNDTNEEQ EKAVTKIEII SHMKQSTSCL DSEEIRENQG 1390 1400 1410 1420 1430 1440 EVILEVQETY CETSGKLPSE QVLQQPMNAR VKSKREKREV IVEESIRTCK PAFLSEEKLY 1450 1460 1470 1480 1490 1500 QETAIRDEHD SHIKSQPREM TSSEKGKEAD TEMGFSITFK IEESSSQVVP QGISVKHLDA 1510 1520 1530 1540 1550 1560 LTLFSSKQAN EGKVNNLSLC LTLKPEENLS REIACGAQSE PFPCMTPRPE GLHYQESDGK 1570 1580 1590 1600 1610 1620 AQVTGPSQIS KTDKSFQGTT RQETNYQDSW VTSKTKETKH QISSSNECKE KSYQEVSFDP 1630 1640 1650 1660 1670 1680 ARGLKLEEIT VSRPDSKEVR YLEFSDRKDL HHQGSKSDDK LCGTLKSEIA TQELTGEKFL 1690 1700 1710 1720 1730 1740 EMANPNVAGL EAGSIEDIVT QRGSRVLGSF LPEKLFKGVS QKENTGQQNA IISPTVLETS 1750 1760 1770 1780 1790 1800 EEKTVSLTVC SAVKTEKTPQ EKLRESPGSE QTPFMTAPEG KGNGGVNPEP FRATQNVFTR 1810 1820 1830 1840 1850 1860 QLCLEHDEKL VSYLSLLRNI EMRTKQIQPL ELNLAELQDL LCQAKVLERE LKDLTTLVSQ 1870 1880 1890 1900 1910 1920 ELECVNQIII SQPQEVPAQL LKALEKDAKN LQKSLSSVSD TWNSRLLHFQ NAVEIEKTKV 1930 1940 1950 1960 1970 1980 LNQHTQLEGR LQDLRAWVGN KNLILNSKGS NSEIDVDSLN LCLQQYEDLK QPMAERKAQL 1990 2000 2010 2020 2030 2040 DALAFDIQFF ISEHAQDLSP QQNRQMLRLL NELQRSFQDI LEQTAAQVDA LQGHLQQMEQ 2050 2060 2070 2080 2090 2100 EALVKTLQKQ QNTCHQQLED LCSWVGQAER ALAGHQGRTT QQDLSALQKN QSDLKDLQDD 2110 2120 2130 2140 2150 2160 IQNRATSFAT VVKDIEGFME ENQTKLSPRE LTALREKLHQ AKEQYEALQE ETRVAQKELE 2170 2180 2190 2200 2210 2220 EAVTSALQQE TEKSKAAKEL AENKKKIDAL LDWVTSVGSS GGQLLTNLPG MEQLSGASLE 2230 2240 2250 2260 2270 2280 KGALDTTDGY MGVNQAPEKL DKQCEMMKAR HQELLSQQQN FILATQSAQA FLDQHGHNLT 2290 2300 2310 2320 2330 2340 PEEQQMLQQK LGELKEQYST SLAQSEAELK QVQTLQDELQ KFLQDHKEFE SWLERSEKEL 2350 2360 2370 2380 2390 2400 ENMHKGGSSP ETLPSLLKRQ GSFSEDVISH KGDLRFVTIS GQKVLDMENS FKEGKEPSEI 2410 2420 2430 2440 2450 2460 GNLVKDKLKD ATERYTALHS KCTRLGSHLN MLLGQYHQFQ NSADSLQAWM QACEANVEKL 2470 2480 2490 2500 2510 2520 LSDTVASDPG VLQEQLATTK QLQEELAEHQ VPVEKLQKVA RDIMEIEGEP APDHRHVQET 2530 2540 2550 2560 2570 2580 TDSILSHFQS LSYSLAERSS LLQKAIAQSQ SVQESLESLL QSIGEVEQNL EGKQVSSLSS 2590 2600 2610 2620 2630 2640 GVIQEALATN MKLKQDIARQ KSSLEATREM VTRFMETADS TTAAVLQGKL AEVSQRFEQL 2650 2660 2670 2680 2690 2700 CLQQQEKESS LKKLLPQAEM FEHLSGKLQQ FMENKSRMLA SGNQPDQDIT HFFQQIQELN 2710 2720 2730 2740 2750 2760 LEMEDQQENL DTLEHLVTEL SSCGFALDLC QHQDRVQNLR KDFTELQKTV KEREKDASSC 2770 2780 2790 2800 2810 2820 QEQLDEFRKL VRTFQKWLKE TEGSIPPTET SMSAKELEKQ IEHLKSLLDD WASKGTLVEE 2830 2840 2850 2860 2870 2880 INCKGTSLEN LIMEITAPDS QGKTGSILPS VGSSVGSVNG YHTCKDLTEI QCDMSDVNLK 2890 2900 2910 2920 2930 2940 YEKLGGVLHE RQESLQAILN RMEEVHKEAN SVLQWLESKE EVLKSMDAMS SPTKTETVKA 2950 2960 2970 2980 2990 3000 QAESNKAFLA ELEQNSPKIQ KVKEALAGLL VTYPNSQEAE NWKKIQEELN SRWERATEVT 3010 3020 3030 3040 3050 3060 VARQRQLEES ASHLACFQAA ESQLRPWLME KELMMGVLGP LSIDPNMLNA QKQQVQFMLK 3070 3080 3090 3100 3110 3120 EFEARRQQHE QLNEAAQGIL TGPGDVSLST SQVQKELQSI NQKWVELTDK LNSRSSQIDQ 3130 3140 3150 3160 3170 3180 AIVKSTQYQE LLQDLSEKVR AVGQRLSVQS AISTQPEAVK QQLEETSEIR SDLEQLDHEV 3190 3200 3210 3220 3230 3240 KEAQTLCDEL SVLIGEQYLK DELKKRLETV ALPLQGLEDL AADRINRLQA ALASTQQFQQ 3250 3260 3270 3280 3290 3300 MFDELRTWLD DKQSQQAKNC PISAKLERLQ SQLQENEEFQ KSLNQHSGSY EVIVAEGESL 3310 3320 3330 3340 3350 3360 LLSVPPGEEK RTLQNQLVEL KNHWEELSKK TADRQSRLKD CMQKAQKYQW HVEDLVPWIE 3370 3380 3390 3400 3410 3420 DCKAKMSELR VTLDPVQLES SLLRSKAMLN EVEKRRSLLE ILNSAADILI NSSEADEDGI 3430 3440 3450 3460 3470 3480 RDEKAGINQN MDAVTEELQA KTGSLEEMTQ RLREFQESFK NIEKKVEGAK HQLEIFDALG 3490 3500 3510 3520 3530 3540 SQACSNKNLE KLRAQQEVLQ ALEPQVDYLR NFTQGLVEDA PDGSDASQLL HQAEVAQQEF 3550 3560 3570 3580 3590 3600 LEVKQRVNSG CVMMENKLEG IGQFHCRVRE MFSQLADLDD ELDGMGAIGR DTDSLQSQIE 3610 3620 3630 3640 3650 3660 DVRLFLNKIH VLKLDIEASE AECRHMLEEE GTLDLLGLKR ELEALNKQCG KLTERGKARQ 3670 3680 3690 3700 3710 3720 EQLELTLGRV EDFYRKLKGL NDATTAAEEA EALQWVVGTE VEIINQQLAD FKMFQKEQVD 3730 3740 3750 3760 3770 3780 PLQMKLQQVN GLGQGLIQSA GKDCDVQGLE HDMEEINARW NTLNKKVAQR IAQLQEALLH 3790 3800 3810 3820 3830 3840 CGKFQDALEP LLSWLADTEE LIANQKPPSA EYKVVKAQIQ EQKLLQRLLD DRKATVDMLQ 3850 3860 3870 3880 3890 3900 AEGGRIAQSA ELADREKITG QLESLESRWT ELLSKAAARQ KQLEDILVLA KQFHETAEPI 3910 3920 3930 3940 3950 3960 SDFLSVTEKK LANSEPVGTQ TAKIQQQIIR HKALEEDIEN HATDVHQAVK IGQSLSSLTS 3970 3980 3990 4000 4010 4020 PAEQGVLSEK IDSLQARYSE IQDRCCRKAA LLDQALSNAR LFGEDEVEVL NWLAEVEDKL 4030 4040 4050 4060 4070 4080 SSVFVKDFKQ DVLHRQHADH LALNEEIVNR KKNVDQAIKN GQALLKQTTG EEVLLIQEKL 4090 4100 4110 4120 4130 4140 DGIKTRYADI TVTSSKALRT LEQARQLATK FQSTYEELTG WLREVEEELA TSGGQSPTGE 4150 4160 4170 4180 4190 4200 QIPQFQQRQK ELKKEVMEHR LVLDTVNEVS RALLELVPWR AREGLDKLVS DANEQYKLVS 4210 4220 4230 4240 4250 4260 DTIGQRVDEI DAAIQRSQQY EQAADAELAW VAETKRKLMA LGPIRLEQDQ TTAQLQVQKA 4270 4280 4290 4300 4310 4320 FSIDIIRHKD SMDELFSHRS EIFGTCGEEQ KTVLQEKTES LIQQYEAISL LNSERYARLE 4330 4340 4350 4360 4370 4380 RAQVLVNQFW ETYEELSPWI EETRALIAQL PSPAIDHEQL RQQQEEMRQL RESIAEHKPH 4390 4400 4410 4420 4430 4440 IDKLLKIGPQ LKELNPEEGE MVEEKYQKAE NMYAQIKEEV RQRALALDEA VSQSTQITEF 4450 4460 4470 4480 4490 4500 HDKIEPMLET LENLSSRLRM PPLIPAEVDK IRECISDNKS ATVELEKLQP SFEALKRRGE 4510 4520 4530 4540 4550 4560 ELIGRSQGAD KDLAAKEIQD KLDQMVFFWE DIKARAEERE IKFLDVLELA EKFWYDMAAL 4570 4580 4590 4600 4610 4620 LTTIKDTQDI VHDLESPGID PSIIKQQVEA AETIKEETDG LHEELEFIRI LGADLIFACG 4630 4640 4650 4660 4670 4680 ETEKPEVRKS IDEMNNAWEN LNKTWKERLE KLEDAMQAAV QYQDTLQAMF DWLDNTVIKL 4690 4700 4710 4720 4730 4740 CTMPPVGTDL NTVKDQLNEM KEFKVEVYQQ QIEMEKLNHQ GELMLKKATD ETDRDIIREP 4750 4760 4770 4780 4790 4800 LTELKHLWEN LGEKIAHRQH KLEGALLALG QFQHALEELM SWLTHTEELL DAQRPISGDP 4810 4820 4830 4840 4850 4860 KVIEVELAKH HVLKNDVLAH QATVETVNKA GNELLESSAG DDASSLRSRL EAMNQCWESV 4870 4880 4890 4900 4910 4920 LQKTEEREQQ LQSTLQQAQG FHSEIEDFLL ELTRMESQLS ASKPTGGLPE TAREQLDTHM 4930 4940 4950 4960 4970 4980 ELYSQLKAKE ETYNQLLDKG RLMLLSRDDS GSGSKTEQSV ALLEQKWHVV SSKMEERKSK 4990 5000 5010 5020 5030 5040 LEEALNLATE FQNSLQEFIN WLTLAEQSLN IASPPSLILN TVLSQIEEHK VFANEVNAHR 5050 5060 5070 5080 5090 5100 DQIIELDQTG NQLKFLSQKQ DVVLIKNLLV SVQSRWEKVV QRSIERGRSL DDARKRAKQF 5110 5120 5130 5140 5150 5160 HEAWKKLIDW LEDAESHLDS ELEISNDPDK IKLQLSKHKE FQKTLGGKQP VYDTTIRTGR 5170 5180 5190 5200 5210 5220 ALKEKTLLPE DSQKLDNFLG EVRDKWDTVC GKSVERQHKL EEALLFSGQF MDALQALVDW 5230 5240 5250 5260 5270 5280 LYKVEPQLAE DQPVHGDLDL VMNLMDAHKV FQKELGKRTG TVQVLKRSGR ELIENSRDDT 5290 5300 5310 5320 5330 5340 TWVKGQLQEL STRWDTVCKL SVSKQSRLEQ ALKQAEVFRD TVHMLLEWLS EAEQTLRFRG 5350 5360 5370 5380 5390 5400 ALPDDTEALQ SLIDTHKEFM KKVEEKRVDV NSAVAMGEVI LAVCHPDCIT TIKHWITIIR 5410 5420 5430 5440 5450 5460 ARFEEVLTWA KQHQQRLETA LSELVANAEL LEELLAWIQW AETTLIQRDQ EPIPQNIDRV 5470 5480 5490 5500 5510 5520 KALIAEHQTF MEEMTRKQPD VDRVTKTYKR KNIEPTHAPF IEKSRSGGRK SLSQPTPPPM 5530 5540 5550 5560 5570 5580 PILSQSEAKN PRINQLSARW QQVWLLALER QRKLNDALDR LEELKEFANF DFDVWRKKYM 5590 5600 5610 5620 5630 5640 RWMNHKKSRV MDFFRRIDKD QDGKITRQEF IDGILASKFP TTKLEMTAVA DIFDRDGDGY 5650 5660 5670 5680 5690 5700 IDYYEFVAAL HPNKDAYRPT TDADKIEDEV TRQVAQCKCA KRFQVEQIGE NKYRFFLGNQ 5710 5720 5730 5740 5750 5760 FGDSQQLRLV RILRSTVMVR VGGGWMALDE FLVKNDPCRA RGRTNIELRE KFILPEGASQ 5770 5780 5790 5800 5810 5820 GMTPFRSRGR RSKPSSRAAS PTRSSSSASQ SNHSCTSMPS SPATPASGTK VIPSSGSKLK 5830 5840 5850 5860 5870 5880 RPTPTFHSSR TSLAGDTSNS SSPASTGAKT NRADPKKSAS RPGSRAGSRA GSRASSRRGS 5890 5900 5910 5920 5930 DASDFDLLET QSACSDTSES SAAGGQGNSR RGLNKPSKIP TMSKKTTTAS PRTPGPKR
	Isoform 1 (Macf1b) [UniParc]. See Q9UPN3.		–	–
	Isoform 2 (Mac1fa) [UniParc]. See Q9UPN3.		–	–
	Isoform 3 [UniParc]. See Q9UPN3.		–	–
	Isoform 5 [UniParc]. See Q9UPN3.		–	–

References

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[1]	"MACF1 gene structure: a hybrid of plectin and dystrophin." Gong T.-W.L., Besirli C.G., Lomax M.I. Mamm. Genome 12:852-861(2001) [PubMed: 11845288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANT THR-5172.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC." Gevaert K., Staes A., Van Damme J., De Groot S., Hugelier K., Demol H., Martens L., Goethals M., Vandekerckhove J. Proteomics 5:3589-3599(2005) [PubMed: 16097034] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5627, MASS SPECTROMETRY. Tissue: Hepatoma.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5823, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1554, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2576; SER-2577 AND SER-2579, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2362; SER-2930; SER-2931; SER-2956; SER-5797; SER-5801; THR-5804 AND SER-5842, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-4754, MASS SPECTROMETRY.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF317696 mRNA. Translation: AAL09459.1. AF325341 , AF325333, AF325334, AF325335, AF325336, AF325339, AF325340 Genomic DNA. Translation: AAL38997.1. AL442071, AL137853, AL365277 Genomic DNA. Translation: CAH73671.1. AL365277, AL137853, AL442071 Genomic DNA. Translation: CAI16422.1. AL137853, AL365277, AL442071 Genomic DNA. Translation: CAI21816.1.
IPI	IPI00432363.
RefSeq	NP_036222.3. NM_012090.4.
UniGene	Hs.472475.
3D structure databases
ProteinModelPortal	Q96PK2.
SMR	Q96PK2. Positions 1-354, 735-1198, 3780-3953, 4624-4872, 4953-5092, 5208-5418, 5574-5651, 5658-5761.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-50616N.
IntAct	Q96PK2. 4 interactions.
STRING	Q96PK2.
PTM databases
PhosphoSite	Q96PK2.
Polymorphism databases
DMDM	296434581.
Proteomic databases
PRIDE	Q96PK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000289893; ENSP00000289893; ENSG00000127603.
GeneID	23499.
KEGG	hsa:23499.
Organism-specific databases
CTD	23499.
GeneCards	GC01P039546.
H-InvDB	HIX0000455.
HGNC	HGNC:13664. MACF1.
HPA	HPA013713.
MIM	608271. gene.
neXtProt	NX_Q96PK2.
PharmGKB	PA30518.
HUGE	Search... Search...
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00600000084106.
HOGENOM	HBG716327.
HOVERGEN	HBG031127.
PhylomeDB	Q96PK2.
Gene expression databases
ArrayExpress	Q96PK2.
Bgee	Q96PK2.
CleanEx	HS_MACF1.
Genevestigator	Q96PK2.
GermOnline	ENSG00000127603. Homo sapiens.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR003108. GAS2_dom. IPR001101. Plectin_repeat. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.30.920.20. GAS2_dom. 1 hit.
Pfam	PF02187. GAS2. 1 hit. PF00681. Plectin. 11 hits. PF00435. Spectrin. 18 hits. [Graphical view]
SMART	SM00054. EFh. 2 hits. SM00243. GAS2. 1 hit. SM00250. PLEC. 19 hits. SM00150. SPEC. 30 hits. [Graphical view]
SUPFAM	SSF143575. SSF143575. 1 hit.
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS51460. GAR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	45871.
SOURCE	Search...

Entry information

Entry name

MACF4_HUMAN

Accession

Primary (citable) accession number: Q96PK2
Secondary accession number(s): B1ALC5, Q8WXY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.