Q96PH1 (NOX5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH oxidase 5
EC=1.6.3.-

Gene names

Name:

NOX5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Ref.1 Ref.10 Ref.14
Cofactor	FAD. Ref.11 Magnesium. Ref.11
Enzyme regulation	Activated by calcium which induces conformational changes and interaction between the N-terminal regulatory region and the C-terminal catalytic region. Inhibited by diphenylene iodonium. Ref.11
Subcellular location	Membrane; Multi-pass membrane protein Potential Ref.14. Isoform v2: Endoplasmic reticulum Ref.14. Isoform v5: Endoplasmic reticulum Ref.14.
Tissue specificity	Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level). Ref.1 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14
Developmental stage	Expressed in fetal tissues. Ref.9
Induction	Down-regulated by TGFB1. Ref.11 Ref.12
Domain	Isoform v1 and isoform v2 of this protein have four functional EF-hand calcium-binding domains. Isoform v3 and isoform v4 have the third EF-hand domain interrupted by an insert.
Sequence similarities	Contains 4 EF-hand domains. Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain.
Biophysicochemical properties	Kinetic parameters: KM=1.06 µM for calcium Ref.11
Sequence caution	The sequence AAG33638.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB15319.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB84897.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Angiogenesis Electron transport Ion transport Transport
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium FAD Metal-binding NADP
Molecular function	Ion channel Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from direct assay Ref.14. Source: UniProtKB cytokine secretion Non-traceable author statement Ref.1. Source: UniProtKB cytokinesis Non-traceable author statement Ref.1. Source: UniProtKB electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW endothelial cell proliferation Inferred from direct assay Ref.14. Source: UniProtKB induction of apoptosis Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of reactive oxygen species metabolic process Inferred from direct assay Ref.14. Source: UniProtKB regulation of fusion of sperm to egg plasma membrane Non-traceable author statement Ref.1. Source: UniProtKB regulation of proton transport Non-traceable author statement Ref.1. Source: UniProtKB superoxide anion generation Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.14. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NADP binding Non-traceable author statement Ref.1. Source: UniProtKB calcium ion binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Non-traceable author statement Ref.1. Source: UniProtKB heme binding Non-traceable author statement Ref.1. Source: UniProtKB hydrogen ion channel activity Inferred from direct assay Ref.1. Source: UniProtKB superoxide-generating NADPH oxidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform v3 (identifier: Q96PH1-1)

Also known as: NOX5gamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform v1 (identifier: Q96PH1-3)

Also known as: NOX5alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     109-136: Missing.

Isoform v2 (identifier: Q96PH1-4)

Also known as: NOX5beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     109-136: Missing.

Isoform v4 (identifier: Q96PH1-2)

Also known as: NOX5delta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Isoform v5 (identifier: Q96PH1-5)

Also known as: NOX5epsilon; NOX5S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-200: Missing.

Isoform v6 (identifier: Q96PH1-6)

Also known as: NOX5zeta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MNTSGDPAQTGPEGCRG → MAFVCAGLSD
     109-136: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	NADPH oxidase 5		PRO_0000224995
Regions
	Topological domain	1 – 238	238	Cytoplasmic Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Topological domain	260 – 266	7	Extracellular Potential
	Transmembrane	267 – 289	23	Helical; Potential
	Topological domain	290 – 317	28	Cytoplasmic Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Topological domain	339 – 362	24	Extracellular Potential
	Transmembrane	363 – 383	21	Helical; Potential
	Topological domain	384 – 394	11	Cytoplasmic Potential
	Transmembrane	395 – 417	23	Helical; Potential
	Topological domain	418 – 434	17	Extracellular Potential
	Transmembrane	435 – 455	21	Helical; Potential
	Topological domain	456 – 583	128	Cytoplasmic Potential
	Transmembrane	584 – 604	21	Helical; Potential
	Topological domain	605 – 765	161	Extracellular Potential
	Domain	26 – 56	31	EF-hand 1
	Domain	57 – 92	36	EF-hand 2
	Domain	93 – 156	64	EF-hand 3; atypical; contains an insert of 28 residues
	Domain	165 – 200	36	EF-hand 4
	Domain	293 – 440	148	Ferric oxidoreductase
	Domain	441 – 577	137	FAD-binding FR-type
	Calcium binding	39 – 49	11	1 Potential
	Calcium binding	70 – 81	12	2 Potential
	Calcium binding	106 – 108	3	3; first part Potential
	Calcium binding	137 – 146	10	3; second part Potential
	Calcium binding	178 – 189	12	4 Potential
	Region	1 – 169	169	N-terminal regulatory region; interacts with the C-terminal catalytic region in a calcium-dependent manner
	Region	449 – 565	117	C-terminal catalytic region
	Compositional bias	374 – 380	7	Poly-Leu
Natural variations
	Alternative sequence	1 – 200	200	Missing in isoform v5.		VSP_017326
	Alternative sequence	1 – 18	18	Missing in isoform v2 and isoform v4.		VSP_017327
	Alternative sequence	1 – 17	17	MNTSG…EGCRG → MAFVCAGLSD in isoform v6.		VSP_041619
	Alternative sequence	109 – 136	28	Missing in isoform v1, isoform v2 and isoform v6.		VSP_017328
	Natural variant	576	1	R → H. Corresponds to variant rs2277552 [ dbSNP | Ensembl ].		VAR_055820
	Natural variant	759	1	R → G. Corresponds to variant rs7168025 [ dbSNP | Ensembl ].		VAR_055821
Experimental info
	Mutagenesis	49	1	E → Q: Loss of binding of 1 calcium molecule. No effect on catalytic activity. Ref.11
	Sequence conflict	208	1	A → T in AAG33638. Ref.8
	Sequence conflict	272	1	G → S in AAI25099. Ref.7
	Sequence conflict	375	1	L → P in BAG37241. Ref.3
	Sequence conflict	380	1	L → F in BAB84884. Ref.2
	Sequence conflict	380	1	L → F in BAB84897. Ref.2
	Sequence conflict	380	1	L → F in BAB15319. Ref.3
	Sequence conflict	380	1	L → F in BAG37241. Ref.3
	Sequence conflict	380	1	L → F in EAW77830. Ref.6
	Sequence conflict	380	1	L → F in AAI25098. Ref.7
	Sequence conflict	380	1	L → F in AAI25099. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform v3 (NOX5gamma) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 96B57225BF919682	FASTA	765	86,439
	10 20 30 40 50 60 MNTSGDPAQT GPEGCRGTMS AEEDARWLRW VTQQFKTIAG EDGEISLQEF KAALHVKESF 70 80 90 100 110 120 FAERFFALFD SDRSGTITLQ ELQEALTLLI HGSPMDKLKF LFQVYDIDVC ARQGASAGTE 130 140 150 160 170 180 WGAGAGPHWA SSPLGTGSGS IDPDELRTVL QSCLRESAIS LPDEKLDQLT LALFESADAD 190 200 210 220 230 240 GNGAITFEEL RDELQRFPGV MENLTISAAH WLTAPAPRPR PRRPRQLTRA YWHNHRSQLF 250 260 270 280 290 300 CLATYAGLHV LLFGLAASAH RDLGASVMVA KGCGQCLNFD CSFIAVLMLR RCLTWLRATW 310 320 330 340 350 360 LAQVLPLDQN IQFHQLMGYV VVGLSLVHTV AHTVNFVLQA QAEASPFQFW ELLLTTRPGI 370 380 390 400 410 420 GWVHGSASPT GVALLLLLLL MFICSSSCIR RSGHFEVFYW THLSYLLVWL LLIFHGPNFW 430 440 450 460 470 480 KWLLVPGILF FLEKAIGLAV SRMAAVCIME VNLLPSKVTH LLIKRPPFFH YRPGDYLYLN 490 500 510 520 530 540 IPTIARYEWH PFTISSAPEQ KDTIWLHIRS QGQWTNRLYE SFKASDPLGR GSKRLSRSVT 550 560 570 580 590 600 MRKSQRSSKG SEILLEKHKF CNIKCYIDGP YGTPTRRIFA SEHAVLIGAG IGITPFASIL 610 620 630 640 650 660 QSIMYRHQKR KHTCPSCQHS WIEGVQDNMK LHKVDFIWIN RDQRSFEWFV SLLTKLEMDQ 670 680 690 700 710 720 AEEAQYGRFL ELHMYMTSAL GKNDMKAIGL QMALDLLANK EKKDSITGLQ TRTQPGRPDW 730 740 750 760 SKVFQKVAAE KKGKVQVFFC GSPALAKVLK GHCEKFGFRF FQENF
	Isoform v1 (NOX5alpha) [UniParc]. Checksum: 33B3AC250C36F6E9	FASTA	737	83,774
	10 20 30 40 50 60 MNTSGDPAQT GPEGCRGTMS AEEDARWLRW VTQQFKTIAG EDGEISLQEF KAALHVKESF 70 80 90 100 110 120 FAERFFALFD SDRSGTITLQ ELQEALTLLI HGSPMDKLKF LFQVYDIDGS GSIDPDELRT 130 140 150 160 170 180 VLQSCLRESA ISLPDEKLDQ LTLALFESAD ADGNGAITFE ELRDELQRFP GVMENLTISA 190 200 210 220 230 240 AHWLTAPAPR PRPRRPRQLT RAYWHNHRSQ LFCLATYAGL HVLLFGLAAS AHRDLGASVM 250 260 270 280 290 300 VAKGCGQCLN FDCSFIAVLM LRRCLTWLRA TWLAQVLPLD QNIQFHQLMG YVVVGLSLVH 310 320 330 340 350 360 TVAHTVNFVL QAQAEASPFQ FWELLLTTRP GIGWVHGSAS PTGVALLLLL LLMFICSSSC 370 380 390 400 410 420 IRRSGHFEVF YWTHLSYLLV WLLLIFHGPN FWKWLLVPGI LFFLEKAIGL AVSRMAAVCI 430 440 450 460 470 480 MEVNLLPSKV THLLIKRPPF FHYRPGDYLY LNIPTIARYE WHPFTISSAP EQKDTIWLHI 490 500 510 520 530 540 RSQGQWTNRL YESFKASDPL GRGSKRLSRS VTMRKSQRSS KGSEILLEKH KFCNIKCYID 550 560 570 580 590 600 GPYGTPTRRI FASEHAVLIG AGIGITPFAS ILQSIMYRHQ KRKHTCPSCQ HSWIEGVQDN 610 620 630 640 650 660 MKLHKVDFIW INRDQRSFEW FVSLLTKLEM DQAEEAQYGR FLELHMYMTS ALGKNDMKAI 670 680 690 700 710 720 GLQMALDLLA NKEKKDSITG LQTRTQPGRP DWSKVFQKVA AEKKGKVQVF FCGSPALAKV 730 LKGHCEKFGF RFFQENF
	Isoform v2 (NOX5beta) [UniParc]. Checksum: 04F7BA7C78B1AEDB	FASTA	719	82,013
	10 20 30 40 50 60 MSAEEDARWL RWVTQQFKTI AGEDGEISLQ EFKAALHVKE SFFAERFFAL FDSDRSGTIT 70 80 90 100 110 120 LQELQEALTL LIHGSPMDKL KFLFQVYDID GSGSIDPDEL RTVLQSCLRE SAISLPDEKL 130 140 150 160 170 180 DQLTLALFES ADADGNGAIT FEELRDELQR FPGVMENLTI SAAHWLTAPA PRPRPRRPRQ 190 200 210 220 230 240 LTRAYWHNHR SQLFCLATYA GLHVLLFGLA ASAHRDLGAS VMVAKGCGQC LNFDCSFIAV 250 260 270 280 290 300 LMLRRCLTWL RATWLAQVLP LDQNIQFHQL MGYVVVGLSL VHTVAHTVNF VLQAQAEASP 310 320 330 340 350 360 FQFWELLLTT RPGIGWVHGS ASPTGVALLL LLLLMFICSS SCIRRSGHFE VFYWTHLSYL 370 380 390 400 410 420 LVWLLLIFHG PNFWKWLLVP GILFFLEKAI GLAVSRMAAV CIMEVNLLPS KVTHLLIKRP 430 440 450 460 470 480 PFFHYRPGDY LYLNIPTIAR YEWHPFTISS APEQKDTIWL HIRSQGQWTN RLYESFKASD 490 500 510 520 530 540 PLGRGSKRLS RSVTMRKSQR SSKGSEILLE KHKFCNIKCY IDGPYGTPTR RIFASEHAVL 550 560 570 580 590 600 IGAGIGITPF ASILQSIMYR HQKRKHTCPS CQHSWIEGVQ DNMKLHKVDF IWINRDQRSF 610 620 630 640 650 660 EWFVSLLTKL EMDQAEEAQY GRFLELHMYM TSALGKNDMK AIGLQMALDL LANKEKKDSI 670 680 690 700 710 TGLQTRTQPG RPDWSKVFQK VAAEKKGKVQ VFFCGSPALA KVLKGHCEKF GFRFFQENF
	Isoform v4 (NOX5delta) [UniParc]. Checksum: 86B46366672B6E4A	FASTA	747	84,678
	10 20 30 40 50 60 MSAEEDARWL RWVTQQFKTI AGEDGEISLQ EFKAALHVKE SFFAERFFAL FDSDRSGTIT 70 80 90 100 110 120 LQELQEALTL LIHGSPMDKL KFLFQVYDID VCARQGASAG TEWGAGAGPH WASSPLGTGS 130 140 150 160 170 180 GSIDPDELRT VLQSCLRESA ISLPDEKLDQ LTLALFESAD ADGNGAITFE ELRDELQRFP 190 200 210 220 230 240 GVMENLTISA AHWLTAPAPR PRPRRPRQLT RAYWHNHRSQ LFCLATYAGL HVLLFGLAAS 250 260 270 280 290 300 AHRDLGASVM VAKGCGQCLN FDCSFIAVLM LRRCLTWLRA TWLAQVLPLD QNIQFHQLMG 310 320 330 340 350 360 YVVVGLSLVH TVAHTVNFVL QAQAEASPFQ FWELLLTTRP GIGWVHGSAS PTGVALLLLL 370 380 390 400 410 420 LLMFICSSSC IRRSGHFEVF YWTHLSYLLV WLLLIFHGPN FWKWLLVPGI LFFLEKAIGL 430 440 450 460 470 480 AVSRMAAVCI MEVNLLPSKV THLLIKRPPF FHYRPGDYLY LNIPTIARYE WHPFTISSAP 490 500 510 520 530 540 EQKDTIWLHI RSQGQWTNRL YESFKASDPL GRGSKRLSRS VTMRKSQRSS KGSEILLEKH 550 560 570 580 590 600 KFCNIKCYID GPYGTPTRRI FASEHAVLIG AGIGITPFAS ILQSIMYRHQ KRKHTCPSCQ 610 620 630 640 650 660 HSWIEGVQDN MKLHKVDFIW INRDQRSFEW FVSLLTKLEM DQAEEAQYGR FLELHMYMTS 670 680 690 700 710 720 ALGKNDMKAI GLQMALDLLA NKEKKDSITG LQTRTQPGRP DWSKVFQKVA AEKKGKVQVF 730 740 FCGSPALAKV LKGHCEKFGF RFFQENF
	Isoform v5 (NOX5epsilon) (NOX5S) [UniParc]. Checksum: 94785B98A61214F6	FASTA	565	64,652
	10 20 30 40 50 60 MENLTISAAH WLTAPAPRPR PRRPRQLTRA YWHNHRSQLF CLATYAGLHV LLFGLAASAH 70 80 90 100 110 120 RDLGASVMVA KGCGQCLNFD CSFIAVLMLR RCLTWLRATW LAQVLPLDQN IQFHQLMGYV 130 140 150 160 170 180 VVGLSLVHTV AHTVNFVLQA QAEASPFQFW ELLLTTRPGI GWVHGSASPT GVALLLLLLL 190 200 210 220 230 240 MFICSSSCIR RSGHFEVFYW THLSYLLVWL LLIFHGPNFW KWLLVPGILF FLEKAIGLAV 250 260 270 280 290 300 SRMAAVCIME VNLLPSKVTH LLIKRPPFFH YRPGDYLYLN IPTIARYEWH PFTISSAPEQ 310 320 330 340 350 360 KDTIWLHIRS QGQWTNRLYE SFKASDPLGR GSKRLSRSVT MRKSQRSSKG SEILLEKHKF 370 380 390 400 410 420 CNIKCYIDGP YGTPTRRIFA SEHAVLIGAG IGITPFASIL QSIMYRHQKR KHTCPSCQHS 430 440 450 460 470 480 WIEGVQDNMK LHKVDFIWIN RDQRSFEWFV SLLTKLEMDQ AEEAQYGRFL ELHMYMTSAL 490 500 510 520 530 540 GKNDMKAIGL QMALDLLANK EKKDSITGLQ TRTQPGRPDW SKVFQKVAAE KKGKVQVFFC 550 560 GSPALAKVLK GHCEKFGFRF FQENF
	Isoform v6 (NOX5zeta) [UniParc]. Checksum: 93B21AF3A0A1CE79	FASTA	730	83,109
	10 20 30 40 50 60 MAFVCAGLSD TMSAEEDARW LRWVTQQFKT IAGEDGEISL QEFKAALHVK ESFFAERFFA 70 80 90 100 110 120 LFDSDRSGTI TLQELQEALT LLIHGSPMDK LKFLFQVYDI DGSGSIDPDE LRTVLQSCLR 130 140 150 160 170 180 ESAISLPDEK LDQLTLALFE SADADGNGAI TFEELRDELQ RFPGVMENLT ISAAHWLTAP 190 200 210 220 230 240 APRPRPRRPR QLTRAYWHNH RSQLFCLATY AGLHVLLFGL AASAHRDLGA SVMVAKGCGQ 250 260 270 280 290 300 CLNFDCSFIA VLMLRRCLTW LRATWLAQVL PLDQNIQFHQ LMGYVVVGLS LVHTVAHTVN 310 320 330 340 350 360 FVLQAQAEAS PFQFWELLLT TRPGIGWVHG SASPTGVALL LLLLLMFICS SSCIRRSGHF 370 380 390 400 410 420 EVFYWTHLSY LLVWLLLIFH GPNFWKWLLV PGILFFLEKA IGLAVSRMAA VCIMEVNLLP 430 440 450 460 470 480 SKVTHLLIKR PPFFHYRPGD YLYLNIPTIA RYEWHPFTIS SAPEQKDTIW LHIRSQGQWT 490 500 510 520 530 540 NRLYESFKAS DPLGRGSKRL SRSVTMRKSQ RSSKGSEILL EKHKFCNIKC YIDGPYGTPT 550 560 570 580 590 600 RRIFASEHAV LIGAGIGITP FASILQSIMY RHQKRKHTCP SCQHSWIEGV QDNMKLHKVD 610 620 630 640 650 660 FIWINRDQRS FEWFVSLLTK LEMDQAEEAQ YGRFLELHMY MTSALGKNDM KAIGLQMALD 670 680 690 700 710 720 LLANKEKKDS ITGLQTRTQP GRPDWSKVFQ KVAAEKKGKV QVFFCGSPAL AKVLKGHCEK 730 FGFRFFQENF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Ca(2+)-activated NADPH oxidase in testis, spleen, and lymph nodes." Banfi B., Molnar G., Maturana A., Steger K., Hegedus B., Demaurex N., Krause K.-H. J. Biol. Chem. 276:37594-37601(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS V1; V2; V3 AND V4), FUNCTION, TISSUE SPECIFICITY. Tissue: Spleen and Testis.
[2]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM V5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 98-765 (ISOFORMS V3/V4). Tissue: Spleen.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM V4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-765 (ISOFORMS V1/V2/V3/V4/V6). Tissue: Kidney epithelium and Spleen.
[4]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM V5).
[5]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS V2 AND V6).
[8]	"Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generation." Kawahara T., Ritsick D., Cheng G., Lambeth J.D. J. Biol. Chem. 280:31859-31869(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-765 (ISOFORMS V1/V2/V3/V4/V6). Tissue: Spleen.
[9]	"Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5." Cheng G., Cao Z., Xu X., van Meir E.G., Lambeth J.D. Gene 269:131-140(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[10]	"NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells." Brar S.S., Corbin Z., Kennedy T.P., Hemendinger R., Thornton L., Bommarius B., Arnold R.S., Whorton A.R., Sturrock A.B., Huecksteadt T.P., Quinn M.T., Krenitsky K., Ardie K.G., Lambeth J.D., Hoidal J.R. Am. J. Physiol. 285:C353-C369(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[11]	"Mechanism of Ca2+ activation of the NADPH oxidase 5 (NOX5)." Banfi B., Tirone F., Durussel I., Knisz J., Moskwa P., Molnar G.Z., Krause K.-H., Cox J.A. J. Biol. Chem. 279:18583-18591(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION, COFACTOR, MUTAGENESIS OF GLU-49, BIOPHYSICOCHEMICAL PROPERTIES.
[12]	"NAD(P)H oxidase 4 mediates transforming growth factor-beta1-induced differentiation of cardiac fibroblasts into myofibroblasts." Cucoranu I., Clempus R., Dikalova A., Phelan P.J., Ariyan S., Dikalov S., Sorescu D. Circ. Res. 97:900-907(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[13]	"Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia." Kamiguti A.S., Serrander L., Lin K., Harris R.J., Cawley J.C., Allsup D.J., Slupsky J.R., Krause K.-H., Zuzel M. J. Immunol. 175:8424-8430(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[14]	"NOX5 variants are functionally active in endothelial cells." BelAiba R.S., Djordjevic T., Petry A., Diemer K., Bonello S., Banfi B., Hess J., Pogrebniak A., Bickel C., Gorlach A. Free Radic. Biol. Med. 42:446-459(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF325189 mRNA. Translation: AAK57193.1. AF325190 mRNA. Translation: AAK57194.1. AF353088 mRNA. Translation: AAK57338.1. AF353089 mRNA. Translation: AAK57339.1. AK074058 mRNA. Translation: BAB84884.1. AK074071 mRNA. Translation: BAB84897.1. Different initiation. AK026011 mRNA. Translation: BAB15319.1. Different initiation. AK314689 mRNA. Translation: BAG37241.1. DQ314884 Genomic DNA. Translation: ABC40743.1. AC027088 Genomic DNA. No translation available. AC087639 Genomic DNA. No translation available. CH471082 Genomic DNA. Translation: EAW77830.1. BC125097 mRNA. Translation: AAI25098.1. BC125098 mRNA. Translation: AAI25099.1. AF317889 mRNA. Translation: AAG33638.1. Different initiation.
IPI	IPI00044456. IPI00739004. IPI00739568. IPI00740953. IPI00742061. IPI01020736.
RefSeq	NP_001171708.1. NM_001184779.1. NP_001171709.1. NM_001184780.1. NP_078781.3. NM_024505.3.
UniGene	Hs.657932.
3D structure databases
ProteinModelPortal	Q96PH1.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4651944.
STRING	9606.ENSP00000260364.
Protein family/group databases
PeroxiBase	6024. HsNOx05.
TCDB	5.B.1.1.5. phagocyte (gp91phox) NADPH oxidase family.
Polymorphism databases
DMDM	74717091.
Proteomic databases
PaxDb	Q96PH1.
PRIDE	Q96PH1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260364; ENSP00000454143; ENSG00000255346. ENST00000388866; ENSP00000373518; ENSG00000255346. ENST00000448182; ENSP00000410887; ENSG00000255346. ENST00000455873; ENSP00000416828; ENSG00000255346. ENST00000530406; ENSP00000432440; ENSG00000255346.
GeneID	79400.
KEGG	hsa:79400.
UCSC	uc002arp.2. human. uc002arq.2. human. uc002arr.2. human. uc010bid.2. human.
Organism-specific databases
CTD	79400.
GeneCards	GC15P069222.
H-InvDB	HIX0012384.
HGNC	HGNC:14874. NOX5.
HPA	HPA019362.
MIM	606572. gene.
neXtProt	NX_Q96PH1.
PharmGKB	PA31693.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287712.
HOVERGEN	HBG082052.
InParanoid	Q96PH1.
OMA	LTRAYWH.
Gene expression databases
Bgee	Q96PH1.
CleanEx	HS_NOX5.
Genevestigator	Q96PH1.
GermOnline	ENSG00000137808. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR013112. FAD-bd_8. IPR017927. Fd_Rdtase_FAD-bd. IPR013130. Fe3_Rdtase_TM_dom. IPR013121. Fe_red_NAD-bd_6. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF13202. EF_hand_3. 1 hit. PF13405. EF_hand_4. 1 hit. PF08022. FAD_binding_8. 1 hit. PF01794. Ferric_reduct. 1 hit. PF08030. NAD_binding_6. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q96PH1.
ChEMBL	CHEMBL1926497.
GenomeRNAi	79400.
NextBio	68361.
SOURCE	Search...

Entry information

Entry name

NOX5_HUMAN

Accession

Primary (citable) accession number: Q96PH1
Secondary accession number(s): B2RBJ4 , Q08AN2, Q08AN3, Q8TEQ1, Q8TER4, Q96PH2, Q96PJ8, Q96PJ9, Q9H6E0, Q9HAM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.