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Protein

Testis-specific serine/threonine-protein kinase 2

Gene

TSSK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at 'Ser-288' and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Kineticsi

  1. KM=10 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei41ATPPROSITE-ProRule annotation1
    Active sitei136Proton acceptorPROSITE-ProRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

    GO - Molecular functioni

    • ATP binding Source: UniProtKB
    • magnesium ion binding Source: UniProtKB
    • protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciZFISH:HS11937-MONOMER.
    SignaLinkiQ96PF2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.1)
    Short name:
    TSK-2
    Short name:
    TSK2
    Short name:
    TSSK-2
    Short name:
    Testis-specific kinase 2
    Alternative name(s):
    DiGeorge syndrome protein G
    Short name:
    DGS-G
    Serine/threonine-protein kinase 22B
    Gene namesi
    Name:TSSK2
    Synonyms:DGSG, SPOGA2, STK22B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:11401. TSSK2.

    Subcellular locationi

    GO - Cellular componenti

    • acrosomal vesicle Source: Ensembl
    • centriole Source: UniProtKB-SubCell
    • cytoplasm Source: UniProtKB
    • nucleus Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi23617.
    OpenTargetsiENSG00000206203.
    PharmGKBiPA36208.

    Chemistry databases

    ChEMBLiCHEMBL6014.
    GuidetoPHARMACOLOGYi2258.

    Polymorphism and mutation databases

    BioMutaiTSSK2.
    DMDMi30316269.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000867681 – 358Testis-specific serine/threonine-protein kinase 2Add BLAST358

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ96PF2.
    PeptideAtlasiQ96PF2.
    PRIDEiQ96PF2.

    PTM databases

    iPTMnetiQ96PF2.
    PhosphoSitePlusiQ96PF2.

    Expressioni

    Tissue specificityi

    Testis-specific. Present in mature spermatozoa (at protein level).3 Publications

    Gene expression databases

    BgeeiENSG00000206203.
    CleanExiHS_TSSK2.
    ExpressionAtlasiQ96PF2. baseline and differential.
    GenevisibleiQ96PF2. HS.

    Interactioni

    Subunit structurei

    Interacts with TSSK1B.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TSKSQ9UJT25EBI-852089,EBI-852101
    TSKSQ9UJT2-22EBI-852089,EBI-852113

    Protein-protein interaction databases

    BioGridi117150. 8 interactors.
    IntActiQ96PF2. 5 interactors.
    STRINGi9606.ENSP00000382544.

    Chemistry databases

    BindingDBiQ96PF2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96PF2.
    SMRiQ96PF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiKOG0583. Eukaryota.
    COG0515. LUCA.
    GeneTreeiENSGT00770000120521.
    HOGENOMiHOG000233016.
    HOVERGENiHBG101110.
    InParanoidiQ96PF2.
    KOiK08811.
    OMAiATVNHGS.
    OrthoDBiEOG091G0EZI.
    PhylomeDBiQ96PF2.
    TreeFamiTF352374.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q96PF2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDDATVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT
    60 70 80 90 100
    DFVERFLPRE MDILATVNHG SIIKTYEIFE TSDGRIYIIM ELGVQGDLLE
    110 120 130 140 150
    FIKCQGALHE DVARKMFRQL SSAVKYCHDL DIVHRDLKCE NLLLDKDFNI
    160 170 180 190 200
    KLSDFGFSKR CLRDSNGRII LSKTFCGSAA YAAPEVLQSI PYQPKVYDIW
    210 220 230 240 250
    SLGVILYIMV CGSMPYDDSD IRKMLRIQKE HRVDFPRSKN LTCECKDLIY
    260 270 280 290 300
    RMLQPDVSQR LHIDEILSHS WLQPPKPKAT SSASFKREGE GKYRAECKLD
    310 320 330 340 350
    TKTGLRPDHR PDHKLGAKTQ HRLLVVPENE NRMEDRLAET SRAKDHHISG

    AEVGKAST
    Length:358
    Mass (Da):40,939
    Last modified:April 30, 2003 - v2
    Checksum:iF231433AA78B2FE9
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti279A → P in AAK98531 (PubMed:15044604).Curated1
    Sequence conflicti304G → D in AAH37781 (PubMed:10591208).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_04124127K → R May be associated with infertility. 2 PublicationsCorresponds to variant rs3747052dbSNPEnsembl.1
    Natural variantiVAR_04124261M → V.1 PublicationCorresponds to variant rs35532431dbSNPEnsembl.1
    Natural variantiVAR_041243197Y → C.1 PublicationCorresponds to variant rs56279111dbSNPEnsembl.1
    Natural variantiVAR_051677244E → K.Corresponds to variant rs35048893dbSNPEnsembl.1
    Natural variantiVAR_059770245C → S.Corresponds to variant rs8140743dbSNPEnsembl.1
    Natural variantiVAR_041244280T → M.1 PublicationCorresponds to variant rs1052763dbSNPEnsembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77564 mRNA. No translation available.
    AF362953 mRNA. Translation: AAK98531.1.
    CR456587 mRNA. Translation: CAG30473.1.
    AC004471 Genomic DNA. No translation available.
    BC037781 mRNA. Translation: AAH37781.1.
    CCDSiCCDS13755.1.
    RefSeqiNP_443732.3. NM_053006.4.
    UniGeneiHs.694070.

    Genome annotation databases

    EnsembliENST00000399635; ENSP00000382544; ENSG00000206203.
    GeneIDi23617.
    KEGGihsa:23617.
    UCSCiuc002zow.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77564 mRNA. No translation available.
    AF362953 mRNA. Translation: AAK98531.1.
    CR456587 mRNA. Translation: CAG30473.1.
    AC004471 Genomic DNA. No translation available.
    BC037781 mRNA. Translation: AAH37781.1.
    CCDSiCCDS13755.1.
    RefSeqiNP_443732.3. NM_053006.4.
    UniGeneiHs.694070.

    3D structure databases

    ProteinModelPortaliQ96PF2.
    SMRiQ96PF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi117150. 8 interactors.
    IntActiQ96PF2. 5 interactors.
    STRINGi9606.ENSP00000382544.

    Chemistry databases

    BindingDBiQ96PF2.
    ChEMBLiCHEMBL6014.
    GuidetoPHARMACOLOGYi2258.

    PTM databases

    iPTMnetiQ96PF2.
    PhosphoSitePlusiQ96PF2.

    Polymorphism and mutation databases

    BioMutaiTSSK2.
    DMDMi30316269.

    Proteomic databases

    PaxDbiQ96PF2.
    PeptideAtlasiQ96PF2.
    PRIDEiQ96PF2.

    Protocols and materials databases

    DNASUi23617.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000399635; ENSP00000382544; ENSG00000206203.
    GeneIDi23617.
    KEGGihsa:23617.
    UCSCiuc002zow.2. human.

    Organism-specific databases

    CTDi23617.
    DisGeNETi23617.
    GeneCardsiTSSK2.
    HGNCiHGNC:11401. TSSK2.
    MIMi610710. gene.
    neXtProtiNX_Q96PF2.
    OpenTargetsiENSG00000206203.
    PharmGKBiPA36208.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0583. Eukaryota.
    COG0515. LUCA.
    GeneTreeiENSGT00770000120521.
    HOGENOMiHOG000233016.
    HOVERGENiHBG101110.
    InParanoidiQ96PF2.
    KOiK08811.
    OMAiATVNHGS.
    OrthoDBiEOG091G0EZI.
    PhylomeDBiQ96PF2.
    TreeFamiTF352374.

    Enzyme and pathway databases

    BioCyciZFISH:HS11937-MONOMER.
    SignaLinkiQ96PF2.

    Miscellaneous databases

    GeneWikiiTSSK2.
    GenomeRNAii23617.
    PROiQ96PF2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000206203.
    CleanExiHS_TSSK2.
    ExpressionAtlasiQ96PF2. baseline and differential.
    GenevisibleiQ96PF2. HS.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTSSK2_HUMAN
    AccessioniPrimary (citable) accession number: Q96PF2
    Secondary accession number(s): Q8IY55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: November 30, 2016
    This is version 156 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.