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Protein

Testis-specific serine/threonine-protein kinase 2

Gene

TSSK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at 'Ser-288' and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.3 Publications

Miscellaneous

TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Kineticsi

  1. KM=10 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei41ATPPROSITE-ProRule annotation1
    Active sitei136Proton acceptorPROSITE-ProRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

    GO - Molecular functioni

    • ATP binding Source: UniProtKB
    • magnesium ion binding Source: UniProtKB
    • protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
    Biological processDifferentiation, Spermatogenesis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ96PF2

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.1)
    Short name:
    TSK-2
    Short name:
    TSK2
    Short name:
    TSSK-2
    Short name:
    Testis-specific kinase 2
    Alternative name(s):
    DiGeorge syndrome protein G
    Short name:
    DGS-G
    Serine/threonine-protein kinase 22B
    Gene namesi
    Name:TSSK2
    Synonyms:DGSG, SPOGA2, STK22B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 22

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000206203.4
    HGNCiHGNC:11401 TSSK2
    MIMi610710 gene
    neXtProtiNX_Q96PF2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi23617
    OpenTargetsiENSG00000206203
    PharmGKBiPA36208

    Chemistry databases

    ChEMBLiCHEMBL6014
    GuidetoPHARMACOLOGYi2258

    Polymorphism and mutation databases

    BioMutaiTSSK2
    DMDMi30316269

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000867681 – 358Testis-specific serine/threonine-protein kinase 2Add BLAST358

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ96PF2
    PeptideAtlasiQ96PF2
    PRIDEiQ96PF2

    PTM databases

    iPTMnetiQ96PF2
    PhosphoSitePlusiQ96PF2

    Expressioni

    Tissue specificityi

    Testis-specific. Present in mature spermatozoa (at protein level).3 Publications

    Gene expression databases

    BgeeiENSG00000206203
    CleanExiHS_TSSK2
    ExpressionAtlasiQ96PF2 baseline and differential
    GenevisibleiQ96PF2 HS

    Interactioni

    Subunit structurei

    Interacts with TSSK1B.2 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi117150, 8 interactors
    IntActiQ96PF2, 7 interactors
    STRINGi9606.ENSP00000382544

    Chemistry databases

    BindingDBiQ96PF2

    Structurei

    3D structure databases

    ProteinModelPortaliQ96PF2
    SMRiQ96PF2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG0583 Eukaryota
    COG0515 LUCA
    GeneTreeiENSGT00770000120521
    HOGENOMiHOG000233016
    HOVERGENiHBG101110
    InParanoidiQ96PF2
    KOiK08811
    OMAiEGKYRAE
    OrthoDBiEOG091G0EZI
    PhylomeDBiQ96PF2
    TreeFamiTF352374

    Family and domain databases

    InterProiView protein in InterPro
    IPR011009 Kinase-like_dom_sf
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    IPR008271 Ser/Thr_kinase_AS
    PfamiView protein in Pfam
    PF00069 Pkinase, 1 hit
    SMARTiView protein in SMART
    SM00220 S_TKc, 1 hit
    SUPFAMiSSF56112 SSF56112, 1 hit
    PROSITEiView protein in PROSITE
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit
    PS00108 PROTEIN_KINASE_ST, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q96PF2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDDATVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT
    60 70 80 90 100
    DFVERFLPRE MDILATVNHG SIIKTYEIFE TSDGRIYIIM ELGVQGDLLE
    110 120 130 140 150
    FIKCQGALHE DVARKMFRQL SSAVKYCHDL DIVHRDLKCE NLLLDKDFNI
    160 170 180 190 200
    KLSDFGFSKR CLRDSNGRII LSKTFCGSAA YAAPEVLQSI PYQPKVYDIW
    210 220 230 240 250
    SLGVILYIMV CGSMPYDDSD IRKMLRIQKE HRVDFPRSKN LTCECKDLIY
    260 270 280 290 300
    RMLQPDVSQR LHIDEILSHS WLQPPKPKAT SSASFKREGE GKYRAECKLD
    310 320 330 340 350
    TKTGLRPDHR PDHKLGAKTQ HRLLVVPENE NRMEDRLAET SRAKDHHISG

    AEVGKAST
    Length:358
    Mass (Da):40,939
    Last modified:April 30, 2003 - v2
    Checksum:iF231433AA78B2FE9
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti279A → P in AAK98531 (PubMed:15044604).Curated1
    Sequence conflicti304G → D in AAH37781 (PubMed:10591208).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_04124127K → R May be associated with infertility. 2 PublicationsCorresponds to variant dbSNP:rs3747052Ensembl.1
    Natural variantiVAR_04124261M → V1 PublicationCorresponds to variant dbSNP:rs35532431Ensembl.1
    Natural variantiVAR_041243197Y → C1 PublicationCorresponds to variant dbSNP:rs56279111Ensembl.1
    Natural variantiVAR_051677244E → K. Corresponds to variant dbSNP:rs35048893Ensembl.1
    Natural variantiVAR_059770245C → S. Corresponds to variant dbSNP:rs8140743Ensembl.1
    Natural variantiVAR_041244280T → M1 PublicationCorresponds to variant dbSNP:rs1052763Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77564 mRNA No translation available.
    AF362953 mRNA Translation: AAK98531.1
    CR456587 mRNA Translation: CAG30473.1
    AC004471 Genomic DNA No translation available.
    BC037781 mRNA Translation: AAH37781.1
    CCDSiCCDS13755.1
    RefSeqiNP_443732.3, NM_053006.4
    UniGeneiHs.694070

    Genome annotation databases

    EnsembliENST00000399635; ENSP00000382544; ENSG00000206203
    GeneIDi23617
    KEGGihsa:23617
    UCSCiuc002zow.2 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiTSSK2_HUMAN
    AccessioniPrimary (citable) accession number: Q96PF2
    Secondary accession number(s): Q8IY55
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: May 23, 2018
    This is version 164 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

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