Reviewed, UniProtKB/Swiss-Prot Q96PE3 (INP4A_HUMAN)

Names and origin

Protein names

Recommended name:
    Type I inositol-3,4-bisphosphate 4-phosphatase
    EC=3.1.3.66
Alternative name(s):
    Inositol polyphosphate 4-phosphatase type I

Gene names

Name:

INPP4A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
Catalytic activity	1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate.
Pathway	Signal transduction; phosphatidylinositol signaling pathway.
Tissue specificity	Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain. Ref.3
Sequence similarities	Belongs to the inositol-3,4-bisphosphate 4-phosphatase family. Contains 1 C2 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	signal transduction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	phosphatidyl-inositol-4,5-bisphosphate 4-phosphatase activity Inferred from electronic annotation. Source: EC phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96PE3-1)

Also known as: Alpha-3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96PE3-2)

Also known as: Alpha-1;

The sequence of this isoform differs from the canonical sequence as follows:
     574-613: GNPDSHAYWIRPEDPFCDVPSSPCPSTMPSTACHPHLTTH → D

Isoform 3 (identifier: Q96PE3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     389-393: Missing.

Isoform 4 (identifier: Q96PE3-4)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     574-613: GNPDSHAYWIRPEDPFCDVPSSPCPSTMPSTACHPHLTTH → D
     935-977: EGCRRENTMK...PEGTYGKVET → IGTREVVTQK...AYLVTKLRCK

Note: Inactive.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Type I inositol-3,4-bisphosphate 4-phosphatase		PRO_0000190232
Regions
	Domain	31 – 137	107	C2
Natural variations
	Alternative sequence	389 – 393	5	Missing in isoform 3.		VSP_015240
	Alternative sequence	574 – 613	40	GNPDS…HLTTH → D in isoform 2 and isoform 4.		VSP_015241
	Alternative sequence	935 – 977	43	EGCRR…GKVET → IGTREVVTQKNLSGLVPIRD LRLDPSLLCSIPLLALSPNL LIVWLFLSIAYLVTKLRCK in isoform 4.		VSP_015242
Experimental info
	Sequence conflict	767	1	G → E in AAH28361. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha-3) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 14087B24256B2D05	FASTA	977	109,956
	10 20 30 40 50 60 MTAREHSPRH GARARAMQRA STIDVAADML GLSLAGNIQD PDEPILEFSL ACSELHTPSL 70 80 90 100 110 120 DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQVKLSVY 130 140 150 160 170 180 DVKDRSQGTM YLLGSGTFIV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQR 190 200 210 220 230 240 PPVTRSVDTV NGRMVLPVDE SLTEALGIRS KYASLRKDTL LKSVFGGAIC RMYRFPTTDG 250 260 270 280 290 300 NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT 310 320 330 340 350 360 IILTYQENLT DLHQYRGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDIVTI 370 380 390 400 410 420 GAPAAHCQGF KSGGLRKKLH KFEETKKHFE ECCTSSGCQS IIYIPQDVVR AKEIIAQINT 430 440 450 460 470 480 LKTQVSYYAE RLSRAAKDRS ATGLERTLAI LADKTRQLVT VCDCKLLANS IHGLNAARPD 490 500 510 520 530 540 YIASKASPTS TEEEQVMLRN DQDTLMARWT GRNSRSSLQV DWHEEEWEKV WLNVDKSLEC 550 560 570 580 590 600 IIQRVDKLLQ KERLHGEGCE DVFPCAGSCT SKKGNPDSHA YWIRPEDPFC DVPSSPCPST 610 620 630 640 650 660 MPSTACHPHL TTHCSPPPEE SSPGEWSEAL YPLLTTLTDC VAMMSDKAKK AMVFLLMQDS 670 680 690 700 710 720 APTIATYLSL QYRRDVVFCQ TLTALICGFI IKLRNCLHDD GFLRQLYTIG LLAQFESLLS 730 740 750 760 770 780 TYGEELAMLE DMSLGIMDLR NVTFKVTQAT SSASADMLPV ITGNRDGFNV RVPLPGPLFD 790 800 810 820 830 840 ALPREIQSGM LLRVQPVLFN VGINEQQTLA ERFGDTSLQE VINVESLVRL NSYFEQFKEV 850 860 870 880 890 900 LPEDCLPRSR SQTCLPELLR FLGQNVHARK NKNVDILWQA AEICRRLNGV RFTSCKSAKD 910 920 930 940 950 960 RTAMSVTLEQ CLILQHEHGM APQVFTQALE CMRSEGCRRE NTMKNVGSRK YAFNSLQLKA 970 FPKHYRPPEG TYGKVET
	Isoform 2 (Alpha-1). Checksum: 02E47A7528C9C4F7	FASTA	938	105,699
	10 20 30 40 50 60 MTAREHSPRH GARARAMQRA STIDVAADML GLSLAGNIQD PDEPILEFSL ACSELHTPSL 70 80 90 100 110 120 DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQVKLSVY 130 140 150 160 170 180 DVKDRSQGTM YLLGSGTFIV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQR 190 200 210 220 230 240 PPVTRSVDTV NGRMVLPVDE SLTEALGIRS KYASLRKDTL LKSVFGGAIC RMYRFPTTDG 250 260 270 280 290 300 NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT 310 320 330 340 350 360 IILTYQENLT DLHQYRGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDIVTI 370 380 390 400 410 420 GAPAAHCQGF KSGGLRKKLH KFEETKKHFE ECCTSSGCQS IIYIPQDVVR AKEIIAQINT 430 440 450 460 470 480 LKTQVSYYAE RLSRAAKDRS ATGLERTLAI LADKTRQLVT VCDCKLLANS IHGLNAARPD 490 500 510 520 530 540 YIASKASPTS TEEEQVMLRN DQDTLMARWT GRNSRSSLQV DWHEEEWEKV WLNVDKSLEC 550 560 570 580 590 600 IIQRVDKLLQ KERLHGEGCE DVFPCAGSCT SKKDCSPPPE ESSPGEWSEA LYPLLTTLTD 610 620 630 640 650 660 CVAMMSDKAK KAMVFLLMQD SAPTIATYLS LQYRRDVVFC QTLTALICGF IIKLRNCLHD 670 680 690 700 710 720 DGFLRQLYTI GLLAQFESLL STYGEELAML EDMSLGIMDL RNVTFKVTQA TSSASADMLP 730 740 750 760 770 780 VITGNRDGFN VRVPLPGPLF DALPREIQSG MLLRVQPVLF NVGINEQQTL AERFGDTSLQ 790 800 810 820 830 840 EVINVESLVR LNSYFEQFKE VLPEDCLPRS RSQTCLPELL RFLGQNVHAR KNKNVDILWQ 850 860 870 880 890 900 AAEICRRLNG VRFTSCKSAK DRTAMSVTLE QCLILQHEHG MAPQVFTQAL ECMRSEGCRR 910 920 930 ENTMKNVGSR KYAFNSLQLK AFPKHYRPPE GTYGKVET
	Isoform 3. Checksum: 63238BA562D67D76	FASTA	972	109,344
	10 20 30 40 50 60 MTAREHSPRH GARARAMQRA STIDVAADML GLSLAGNIQD PDEPILEFSL ACSELHTPSL 70 80 90 100 110 120 DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQVKLSVY 130 140 150 160 170 180 DVKDRSQGTM YLLGSGTFIV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQR 190 200 210 220 230 240 PPVTRSVDTV NGRMVLPVDE SLTEALGIRS KYASLRKDTL LKSVFGGAIC RMYRFPTTDG 250 260 270 280 290 300 NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT 310 320 330 340 350 360 IILTYQENLT DLHQYRGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDIVTI 370 380 390 400 410 420 GAPAAHCQGF KSGGLRKKLH KFEETKKHTS SGCQSIIYIP QDVVRAKEII AQINTLKTQV 430 440 450 460 470 480 SYYAERLSRA AKDRSATGLE RTLAILADKT RQLVTVCDCK LLANSIHGLN AARPDYIASK 490 500 510 520 530 540 ASPTSTEEEQ VMLRNDQDTL MARWTGRNSR SSLQVDWHEE EWEKVWLNVD KSLECIIQRV 550 560 570 580 590 600 DKLLQKERLH GEGCEDVFPC AGSCTSKKGN PDSHAYWIRP EDPFCDVPSS PCPSTMPSTA 610 620 630 640 650 660 CHPHLTTHCS PPPEESSPGE WSEALYPLLT TLTDCVAMMS DKAKKAMVFL LMQDSAPTIA 670 680 690 700 710 720 TYLSLQYRRD VVFCQTLTAL ICGFIIKLRN CLHDDGFLRQ LYTIGLLAQF ESLLSTYGEE 730 740 750 760 770 780 LAMLEDMSLG IMDLRNVTFK VTQATSSASA DMLPVITGNR DGFNVRVPLP GPLFDALPRE 790 800 810 820 830 840 IQSGMLLRVQ PVLFNVGINE QQTLAERFGD TSLQEVINVE SLVRLNSYFE QFKEVLPEDC 850 860 870 880 890 900 LPRSRSQTCL PELLRFLGQN VHARKNKNVD ILWQAAEICR RLNGVRFTSC KSAKDRTAMS 910 920 930 940 950 960 VTLEQCLILQ HEHGMAPQVF TQALECMRSE GCRRENTMKN VGSRKYAFNS LQLKAFPKHY 970 RPPEGTYGKV ET
	Isoform 4 (Beta). Checksum: A0899227C8C4BA85	FASTA	954	107,297
	10 20 30 40 50 60 MTAREHSPRH GARARAMQRA STIDVAADML GLSLAGNIQD PDEPILEFSL ACSELHTPSL 70 80 90 100 110 120 DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQVKLSVY 130 140 150 160 170 180 DVKDRSQGTM YLLGSGTFIV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQR 190 200 210 220 230 240 PPVTRSVDTV NGRMVLPVDE SLTEALGIRS KYASLRKDTL LKSVFGGAIC RMYRFPTTDG 250 260 270 280 290 300 NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT 310 320 330 340 350 360 IILTYQENLT DLHQYRGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDIVTI 370 380 390 400 410 420 GAPAAHCQGF KSGGLRKKLH KFEETKKHFE ECCTSSGCQS IIYIPQDVVR AKEIIAQINT 430 440 450 460 470 480 LKTQVSYYAE RLSRAAKDRS ATGLERTLAI LADKTRQLVT VCDCKLLANS IHGLNAARPD 490 500 510 520 530 540 YIASKASPTS TEEEQVMLRN DQDTLMARWT GRNSRSSLQV DWHEEEWEKV WLNVDKSLEC 550 560 570 580 590 600 IIQRVDKLLQ KERLHGEGCE DVFPCAGSCT SKKDCSPPPE ESSPGEWSEA LYPLLTTLTD 610 620 630 640 650 660 CVAMMSDKAK KAMVFLLMQD SAPTIATYLS LQYRRDVVFC QTLTALICGF IIKLRNCLHD 670 680 690 700 710 720 DGFLRQLYTI GLLAQFESLL STYGEELAML EDMSLGIMDL RNVTFKVTQA TSSASADMLP 730 740 750 760 770 780 VITGNRDGFN VRVPLPGPLF DALPREIQSG MLLRVQPVLF NVGINEQQTL AERFGDTSLQ 790 800 810 820 830 840 EVINVESLVR LNSYFEQFKE VLPEDCLPRS RSQTCLPELL RFLGQNVHAR KNKNVDILWQ 850 860 870 880 890 900 AAEICRRLNG VRFTSCKSAK DRTAMSVTLE QCLILQHEHG MAPQVFTQAL ECMRSIGTRE 910 920 930 940 950 VVTQKNLSGL VPIRDLRLDP SLLCSIPLLA LSPNLLIVWL FLSIAYLVTK LRCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase." Norris F.A., Auethavekiat V., Majerus P.W. J. Biol. Chem. 270:16128-16133(1995) [PubMed: 7608176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family." Norris F.A., Atkins R.C., Majerus P.W. J. Biol. Chem. 272:23859-23864(1997) [PubMed: 9295334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Brain.
[3]	"Identification of a novel spliceoform of inositol polyphosphate 4-phosphatase type Ialpha expressed in human platelets: structure of human inositol polyphosphate 4-phosphatase type I gene." Shearn C.T., Walker J., Norris F.A. Biochem. Biophys. Res. Commun. 286:119-125(2001) [PubMed: 11485317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U26398 mRNA. Translation: AAB01068.1. U96919 mRNA. Translation: AAB72150.1. AF368319 mRNA. Translation: AAK58870.1. AC010134 Genomic DNA. Translation: AAX93230.1. BC028361 mRNA. Translation: AAH28361.1.
IPI	IPI00044388. IPI00215830. IPI00645392. IPI00916254.
PIR	B57487.
RefSeq	NP_001127696.1. NP_001127697.1. NP_001557.1. NP_004018.1.
UniGene	Hs.580527
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q96PE3.
2-D gel databases
OGP	Q13187.
Proteomic databases
PRIDE	Q96PE3.
Genome annotation databases
Ensembl	ENSG00000040933. Homo sapiens. [Contig view]
GeneID	3631.
KEGG	hsa:3631.
Organism-specific databases
GeneCards	GC02P098465.
H-InvDB	HIX0002297.
HGNC	HGNC:6074. INPP4A.
MIM	600916. gene.
PharmGKB	PA29882.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96PE3.
HOVERGEN	Q96PE3.
Enzyme and pathway databases
BRENDA	3.1.3.66. 247.
Gene expression databases
ArrayExpress	Q96PE3.
Bgee	Q96PE3.
CleanEx	HS_INPP4A.
GermOnline	ENSG00000040933. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
PROSITE	PS50004. C2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14207.
SOURCE	Search...

Entry information

Entry name

INP4A_HUMAN

Accession

Primary (citable) accession number: Q96PE3
Secondary accession number(s): O15326 , Q13187, Q53TD8, Q8TC02

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 1, 2001
Last modified:	March 24, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)