Q96PE2 (ARHGH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho guanine nucleotide exchange factor 17

Alternative name(s):
164 kDa Rho-specific guanine-nucleotide exchange factor
Short name=p164-RhoGEF
Short name=p164RhoGEF
Tumor endothelial marker 4

Gene names

Name:	ARHGEF17
Synonyms:	KIAA0337, TEM4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2063 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Ref.6
Tissue specificity	Highly expressed in the heart. Ref.6
Sequence similarities	Contains 1 DH (DBL-homology) domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from direct assay Ref.6. Source: MGI apoptotic process Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro guanyl-nucleotide exchange factor activity Inferred from direct assay Ref.6. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2063	2063	Rho guanine nucleotide exchange factor 17		PRO_0000286589
Regions
	Domain	1066 – 1254	189	DH
	Compositional bias	201 – 204	4	Poly-Gln
	Compositional bias	230 – 235	6	Poly-Ser
	Compositional bias	252 – 256	5	Poly-Glu
	Compositional bias	566 – 569	4	Poly-Ser
	Compositional bias	672 – 675	4	Poly-Ser
	Compositional bias	707 – 710	4	Poly-Arg
	Compositional bias	963 – 1054	92	Pro-rich
	Compositional bias	1990 – 1996	7	Poly-Pro
Amino acid modifications
	Modified residue	142	1	Phosphoserine By similarity
	Modified residue	461	1	Phosphoserine Ref.8
	Modified residue	1002	1	Phosphoserine By similarity
Natural variations
	Natural variant	450	1	G → E. Corresponds to variant rs3741150 [ dbSNP | Ensembl ].		VAR_032132
	Natural variant	1465	1	A → D. Corresponds to variant rs2298808 [ dbSNP | Ensembl ].		VAR_032133
Experimental info
	Sequence conflict	722	1	L → F in BAA20795. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q96PE2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 76A53FF6CBF5F5C0	FASTA	2,063	221,673
	10 20 30 40 50 60 MADGAPRPQL YRSVSFKLLE RWSGGPGLRE EDTDTPGLRR RASCRPTTAA RGQPSRRVSK 70 80 90 100 110 120 LASGPLAAPA QPRPLRSLSP SVRQLSRRFD APRLDDGSAG TRDGGVLPAA AEEAAEGPAR 130 140 150 160 170 180 GAWPSVTEMR KLFGGPGSRR PSADSESPGT PSPDGAAWEP PARESRQPPT PPPRTCFPLA 190 200 210 220 230 240 GLRSARPLTG PETEGRLRRP QQQQERAQRP ADGLHSWHIF SQPQAGARAS CSSSSIAASY 250 260 270 280 290 300 PVSRSRAASS SEEEEEGPPQ LPGAQSPAYH GGHSSGSDDD RDGEGGHRWG GRPGLRPGSS 310 320 330 340 350 360 LLDQDCRPDS DGLNLSSMNS AGVSGSPEPP TSPRAPREEG LREWGSGSPP CVPGPQEGLR 370 380 390 400 410 420 PMSDSVGGAF RVAKVSFPSY LASPAGSRGS SRYSSTETLK DDDLWSSRGS GGWGVYRSPS 430 440 450 460 470 480 FGAGEGLLRS QARTRAKGPG GTSRALRDGG FEPEKSRQRK SLSNPDIASE TLTLLSFLRS 490 500 510 520 530 540 DLSELRVRKP GGSSGDRGSN PLDGRDSPSA GGPVGQLEPI PIPAPASPGT RPTLKDLTAT 550 560 570 580 590 600 LRRAKSFTCS EKPMARRLPR TSALKSSSSE LLLTGPGAEE DPLPLIVQDQ YVQEARQVFE 610 620 630 640 650 660 KIQRMGAQQD DGSDAPPGSP DWAGDVTRGQ RSQEELSGPE SSLTDEGIGA DPEPPVAAFC 670 680 690 700 710 720 GLGTTGMWRP LSSSSAQTNH HGPGTEDSLG GWALVSPETP PTPGALRRRR KVPPSGSGGS 730 740 750 760 770 780 ELSNGEAGEA YRSLSDPIPQ RHRAATSEEP TGFSVDSNLL GSLSPKTGLP ATSAMDEGLT 790 800 810 820 830 840 SGHSDWSVGS EESKGYQEVI QSIVQGPGTL GRVVDDRIAG KAPKKKSLSD PSRRGELAGP 850 860 870 880 890 900 GFEGPGGEPI REVEPMLPPS SSEPILVEQR AEPEEPGATR SRAQSERALP EALPPPATAH 910 920 930 940 950 960 RNFHLDPKLA DILSPRLIRR GSKKRPARSS HQELRRDEGS QDQTGSLSRA RPSSRHVRHA 970 980 990 1000 1010 1020 SVPATFMPIV VPEPPTSVGP PVAVPEPIGF PTRAHPTLQA PSLEDVTKQY MLNLHSGEVP 1030 1040 1050 1060 1070 1080 APVPVDMPCL PLAAPPSAEA KPPEAARPAD EPTPASKCCS KPQVDMRKHV AMTLLDTEQS 1090 1100 1110 1120 1130 1140 YVESLRTLMQ GYMQPLKQPE NSVLCDPSLV DEIFDQIPEL LEHHEQFLEQ VRHCMQTWHA 1150 1160 1170 1180 1190 1200 QQKVGALLVQ SFSKDVLVNI YSAYIDNFLN AKDAVRVAKE ARPAFLKFLE QSMRENKEKQ 1210 1220 1230 1240 1250 1260 ALSDLMIKPV QRIPRYELLV KDLLKHTPED HPDHPLLLEA QRNIKQVAER INKGVRSAEE 1270 1280 1290 1300 1310 1320 AERHARVLQE IEAHIEGMED LQAPLRRFLR QEMVIEVKAI GGKKDRSLFL FTDLIVCTTL 1330 1340 1350 1360 1370 1380 KRKSGSLRRS SMSLYTAASV IDTASKYKML WKLPLEDADI IKGASQATNR ENIQKAISRL 1390 1400 1410 1420 1430 1440 DEDLTTLGQM SKLSESLGFP HQSLDDALRD LSAAMHRDLS EKQALCYALS FPPTKLELCA 1450 1460 1470 1480 1490 1500 TRPEGTDSYI FEFPHPDARL GFEQAFDEAK RKLASSKSCL DPEFLKAIPI MKTRSGMQFS 1510 1520 1530 1540 1550 1560 CAAPTLNSCP EPSPEVWVCN SDGYVGQVCL LSLRAEPDVE ACIAVCSARI LCIGAVPGLQ 1570 1580 1590 1600 1610 1620 PRCHREPPPS LRSPPETAPE PAGPELDVEA AADEEAATLA EPGPQPCLHI SIAGSGLEMT 1630 1640 1650 1660 1670 1680 PGLGEGDPRP ELVPFDSDSD DESSPSPSGT LQSQASRSTI SSSFGNEETP SSKEATAETT 1690 1700 1710 1720 1730 1740 SSEEEQEPGF LPLSGSFGPG GPCGTSPMDG RALRRSSHGS FTRGSLEDLL SVDPEAYQSS 1750 1760 1770 1780 1790 1800 VWLGTEDGCV HVYQSSDSIR DRRNSMKLQH AASVTCILYL NNQVFVSLAN GELVVYQREA 1810 1820 1830 1840 1850 1860 GHFWDPQNFK SVTLGTQGSP ITKMVSVGGR LWCGCQNRVL VLSPDTLQLE HMFYVGQDSS 1870 1880 1890 1900 1910 1920 RCVACMVDSS LGVWVTLKGS AHVCLYHPDT FEQLAEVDVT PPVHRMLAGS DAIIRQHKAA 1930 1940 1950 1960 1970 1980 CLRITALLVC EELLWVGTSA GVVLTMPTSP GTVSCPRAPL SPTGLGQGHT GHVRFLAAVQ 1990 2000 2010 2020 2030 2040 LPDGFNLLCP TPPPPPDTGP EKLPSLEHRD SPWHRGPAPA RPKMLVISGG DGYEDFRLSS 2050 2060 GGGSSSETVG RDDSTNHLLL WRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cell surface tumor endothelial markers are conserved in mice and humans." Carson-Walter E.B., Watkins D.N., Nanda A., Vogelstein B., Kinzler K.W., St Croix B. Cancer Res. 61:6649-6655(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Colon.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-401. Tissue: Embryo.
[5]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 455-2063. Tissue: Brain.
[6]	"A mammalian Rho-specific guanine-nucleotide exchange factor (p164-RhoGEF) without a pleckstrin homology domain." Ruemenapp U., Freichel-Blomquist A., Wittinghofer B., Jakobs K.H., Wieland T. Biochem. J. 366:721-728(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-461, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF378754 mRNA. Translation: AAL11991.1. CH471076 Genomic DNA. Translation: EAW74891.1. BC050031 mRNA. Translation: AAH50031.1. BC137228 mRNA. Translation: AAI37229.1. AK074500 mRNA. No translation available. AB002335 mRNA. Translation: BAA20795.2.
IPI	IPI00152007.
RefSeq	NP_055601.2. NM_014786.3.
UniGene	Hs.533719. Hs.573929.
3D structure databases
ProteinModelPortal	Q96PE2.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3974886.
STRING	9606.ENSP00000263674.
PTM databases
PhosphoSite	Q96PE2.
Polymorphism databases
DMDM	74724250.
Proteomic databases
PaxDb	Q96PE2.
PRIDE	Q96PE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263674; ENSP00000263674; ENSG00000110237.
GeneID	9828.
KEGG	hsa:9828.
UCSC	uc001otu.3. human.
Organism-specific databases
CTD	9828.
GeneCards	GC11P073019.
H-InvDB	HIX0171270.
HGNC	HGNC:21726. ARHGEF17.
HPA	HPA007991.
neXtProt	NX_Q96PE2.
PharmGKB	PA134884537.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG312337.
HOGENOM	HOG000034048.
HOVERGEN	HBG095607.
InParanoid	Q96PE2.
OMA	MGAQQDD.
OrthoDB	EOG4G4GPN.
PhylomeDB	Q96PE2.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q96PE2.
CleanEx	HS_ARHGEF17.
Genevestigator	Q96PE2.
Family and domain databases
Gene3D	1.20.900.10. 1 hit. 2.130.10.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000219. DH-domain. IPR011993. PH_like_dom. IPR015943. WD40/YVTN_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00325. RhoGEF. 1 hit. [Graphical view]
SUPFAM	SSF48065. DH-domain. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9828.
NextBio	37026.

Entry information

Entry name

ARHGH_HUMAN

Accession

Primary (citable) accession number: Q96PE2
Secondary accession number(s): B2RP20 , Q86XU2, Q8N2S0, Q9Y4G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.