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Protein

Adhesion G protein-coupled receptor A2

Gene

ADGRA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endothelial receptor which functions as a WNT7-specific coactivator of canonical Wnt signaling (By similarity). Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). Has a major role in establishing the blood-brain barrier (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).By similarity1 Publication

GO - Molecular functioni

  • G-protein coupled receptor activity Source: GDB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Angiogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor A2Imported
Alternative name(s):
G-protein coupled receptor 124Imported
Tumor endothelial marker 51 Publication
Gene namesi
Name:ADGRA2Imported
Synonyms:GPR124, KIAA1531Imported, TEM51 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:17849. ADGRA2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 771738Extracellular3 PublicationsAdd
BLAST
Transmembranei772 – 79221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini793 – 80715CytoplasmicSequence analysisAdd
BLAST
Transmembranei808 – 82821Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini829 – 8324ExtracellularSequence analysis
Transmembranei833 – 85321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini854 – 88633CytoplasmicSequence analysisAdd
BLAST
Transmembranei887 – 90721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini908 – 92417ExtracellularSequence analysisAdd
BLAST
Transmembranei925 – 94521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini946 – 101974CytoplasmicSequence analysisAdd
BLAST
Transmembranei1020 – 104021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini1041 – 10477ExtracellularSequence analysis
Transmembranei1048 – 106821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini1069 – 1338270Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • cell surface Source: Ensembl
  • filopodium Source: UniProtKB-SubCell
  • integral component of membrane Source: GDB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi369 – 3691R → A: Minimal effect on thrombin cleavage. Abolishes thrombin cleavage; when associated with G-398. 1 Publication
Mutagenesisi398 – 3981R → G: Minimal effect on thrombin cleavage. Abolishes thrombin cleavage; when associated with A-369. 1 Publication
Mutagenesisi1335 – 13384Missing : Fails to interact with DLG1. 1 Publication

Organism-specific databases

PharmGKBiPA134869404.

Polymorphism and mutation databases

BioMutaiGPR124.
DMDMi221222450.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 13381305Adhesion G protein-coupled receptor A2PRO_0000012898Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence analysis
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence analysis
Disulfide bondi268 ↔ 328PROSITE-ProRule annotation
Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence analysis
Glycosylationi602 – 6021N-linked (GlcNAc...)Sequence analysis
Glycosylationi690 – 6901N-linked (GlcNAc...)Sequence analysis
Modified residuei1107 – 11071PhosphoserineCombined sources

Post-translational modificationi

Glycosylated.1 Publication
Proteolytically cleaved into two subunits, an extracellular subunit and a seven-transmembrane subunit (PubMed:22013897, PubMed:16982628). Cleaved by thrombin (F2) and MMP1 (PubMed:22013897). Also cleaved by MMP9, with lower efficiency (PubMed:22013897, PubMed:16982628). Presence of the protein disulfide-isomerase P4HB at the cell surface is additionally required for shedding of the extracellular subunit, suggesting that the subunits are linked by disulfide bonds (PubMed:22013897). Shedding is enhanced by the growth factor FGF2 and may promote cell survival during angiogenesis (PubMed:16982628).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Cleavage; by thrombin1 Publication
Sitei398 – 3992Cleavage; by thrombin1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ96PE1.
PeptideAtlasiQ96PE1.
PRIDEiQ96PE1.

PTM databases

iPTMnetiQ96PE1.
PhosphoSiteiQ96PE1.

Expressioni

Tissue specificityi

Expressed in endothelial cells (at protein level) (PubMed:15021905, PubMed:16982628). Abundantly expressed in heart, placenta, ovary, small intestine, and colon (PubMed:15021905).2 Publications

Gene expression databases

BgeeiQ96PE1.
CleanExiHS_GPR124.
ExpressionAtlasiQ96PE1. baseline and differential.
GenevisibleiQ96PE1. HS.

Organism-specific databases

HPAiHPA012393.

Interactioni

Subunit structurei

Interacts (via PDZ-binding motif) with DLG1 (via PDZ domains) (PubMed:15021905). The cleaved extracellular subunit interacts with the integrin heterodimer ITGAV:ITGB3 (PubMed:16982628).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG1Q12959-22EBI-10893263,EBI-357500

Protein-protein interaction databases

BioGridi117450. 2 interactions.
IntActiQ96PE1. 1 interaction.
STRINGi9606.ENSP00000406367.

Structurei

3D structure databases

ProteinModelPortaliQ96PE1.
SMRiQ96PE1. Positions 38-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 10625LRR 1Sequence analysisAdd
BLAST
Repeati107 – 13024LRR 2Sequence analysisAdd
BLAST
Repeati131 – 15424LRR 3Sequence analysisAdd
BLAST
Repeati156 – 17823LRR 4Sequence analysisAdd
BLAST
Domaini190 – 24051LRRCTSequence analysisAdd
BLAST
Domaini247 – 34498Ig-likePROSITE-ProRule annotationAdd
BLAST
Domaini707 – 75852GPSPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi362 – 3643RGD1 Publication
Motifi1335 – 13384PDZ-binding1 Publication

Domaini

The leucine-rich repeats (LRRs) are important for potentiation of WNT7 signaling.By similarity
The RGD motif is involved in integrin ITGAV:ITGB3 binding.1 Publication

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129728.
HOVERGENiHBG051777.
InParanoidiQ96PE1.
KOiK08461.
OMAiRTLCAYP.
OrthoDBiEOG7RV9F8.
PhylomeDBiQ96PE1.
TreeFamiTF331206.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96PE1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAGGRRMRG APARLLLPLL PWLLLLLAPE ARGAPGCPLS IRSCKCSGER
60 70 80 90 100
PKGLSGGVPG PARRRVVCSG GDLPEPPEPG LLPNGTVTLL LSNNKITGLR
110 120 130 140 150
NGSFLGLSLL EKLDLRNNII STVQPGAFLG LGELKRLDLS NNRIGCLTSE
160 170 180 190 200
TFQGLPRLLR LNISGNIFSS LQPGVFDELP ALKVVDLGTE FLTCDCHLRW
210 220 230 240 250
LLPWAQNRSL QLSEHTLCAY PSALHAQALG SLQEAQLCCE GALELHTHHL
260 270 280 290 300
IPSLRQVVFQ GDRLPFQCSA SYLGNDTRIR WYHNRAPVEG DEQAGILLAE
310 320 330 340 350
SLIHDCTFIT SELTLSHIGV WASGEWECTV SMAQGNASKK VEIVVLETSA
360 370 380 390 400
SYCPAERVAN NRGDFRWPRT LAGITAYQSC LQYPFTSVPL GGGAPGTRAS
410 420 430 440 450
RRCDRAGRWE PGDYSHCLYT NDITRVLYTF VLMPINASNA LTLAHQLRVY
460 470 480 490 500
TAEAASFSDM MDVVYVAQMI QKFLGYVDQI KELVEVMVDM ASNLMLVDEH
510 520 530 540 550
LLWLAQREDK ACSRIVGALE RIGGAALSPH AQHISVNARN VALEAYLIKP
560 570 580 590 600
HSYVGLTCTA FQRREGGVPG TRPGSPGQNP PPEPEPPADQ QLRFRCTTGR
610 620 630 640 650
PNVSLSSFHI KNSVALASIQ LPPSLFSSLP AALAPPVPPD CTLQLLVFRN
660 670 680 690 700
GRLFHSHSNT SRPGAAGPGK RRGVATPVIF AGTSGCGVGN LTEPVAVSLR
710 720 730 740 750
HWAEGAEPVA AWWSQEGPGE AGGWTSEGCQ LRSSQPNVSA LHCQHLGNVA
760 770 780 790 800
VLMELSAFPR EVGGAGAGLH PVVYPCTALL LLCLFATIIT YILNHSSIRV
810 820 830 840 850
SRKGWHMLLN LCFHIAMTSA VFAGGITLTN YQMVCQAVGI TLHYSSLSTL
860 870 880 890 900
LWMGVKARVL HKELTWRAPP PQEGDPALPT PSPMLRFYLI AGGIPLIICG
910 920 930 940 950
ITAAVNIHNY RDHSPYCWLV WRPSLGAFYI PVALILLITW IYFLCAGLRL
960 970 980 990 1000
RGPLAQNPKA GNSRASLEAG EELRGSTRLR GSGPLLSDSG SLLATGSARV
1010 1020 1030 1040 1050
GTPGPPEDGD SLYSPGVQLG ALVTTHFLYL AMWACGALAV SQRWLPRVVC
1060 1070 1080 1090 1100
SCLYGVAASA LGLFVFTHHC ARRRDVRASW RACCPPASPA APHAPPRALP
1110 1120 1130 1140 1150
AAAEDGSPVF GEGPPSLKSS PSGSSGHPLA LGPCKLTNLQ LAQSQVCEAG
1160 1170 1180 1190 1200
AAAGGEGEPE PAGTRGNLAH RHPNNVHHGR RAHKSRAKGH RAGEACGKNR
1210 1220 1230 1240 1250
LKALRGGAAG ALELLSSESG SLHNSPTDSY LGSSRNSPGA GLQLEGEPML
1260 1270 1280 1290 1300
TPSEGSDTSA APLSEAGRAG QRRSASRDSL KGGGALEKES HRRSYPLNAA
1310 1320 1330
SLNGAPKGGK YDDVTLMGAE VASGGCMKTG LWKSETTV
Length:1,338
Mass (Da):142,647
Last modified:January 20, 2009 - v2
Checksum:i322838CB31E99A97
GO
Isoform 2 (identifier: Q96PE1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     537-753: Missing.

Show »
Length:1,121
Mass (Da):119,862
Checksum:i5A118A1292E3DCEE
GO
Isoform 3 (identifier: Q96PE1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-1338: Missing.

Note: No experimental confirmation available.
Show »
Length:185
Mass (Da):19,530
Checksum:i28984931C571C168
GO

Sequence cautioni

The sequence AAI46775.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAL11992.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAO27354.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA96055.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4071G → S in BAB84925 (Ref. 9) Curated
Sequence conflicti1221 – 12211S → G in BAA96055 (PubMed:10819331).Curated
Sequence conflicti1221 – 12211S → G in AAI46775 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291P → L Found in a family with atypical autism and severe epilepsy; unknown pathological significance. 1 Publication
VAR_072079
Natural varianti375 – 3751T → K.1 Publication
VAR_072561

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei186 – 13381153Missing in isoform 3. 1 PublicationVSP_036215Add
BLAST
Alternative sequencei537 – 753217Missing in isoform 2. 2 PublicationsVSP_010076Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378755 mRNA. Translation: AAL11992.1. Different initiation.
AB040964 mRNA. Translation: BAA96055.2. Different initiation.
AK027296 mRNA. Translation: BAB55022.1.
AC138356 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63355.1.
CH471080 Genomic DNA. Translation: EAW63356.1.
BC146774 mRNA. Translation: AAI46775.1. Different initiation.
AY181242 mRNA. Translation: AAO27354.1. Different initiation.
AK074099 mRNA. Translation: BAB84925.2.
AL110244 mRNA. Translation: CAB53694.1.
AL832524 mRNA. Translation: CAD38629.1.
CCDSiCCDS6097.2. [Q96PE1-1]
PIRiT14774.
RefSeqiNP_116166.9. NM_032777.9. [Q96PE1-1]
UniGeneiHs.274136.

Genome annotation databases

EnsembliENST00000315215; ENSP00000323508; ENSG00000020181. [Q96PE1-2]
ENST00000412232; ENSP00000406367; ENSG00000020181. [Q96PE1-1]
GeneIDi25960.
KEGGihsa:25960.
UCSCiuc003xkj.4. human. [Q96PE1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378755 mRNA. Translation: AAL11992.1. Different initiation.
AB040964 mRNA. Translation: BAA96055.2. Different initiation.
AK027296 mRNA. Translation: BAB55022.1.
AC138356 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63355.1.
CH471080 Genomic DNA. Translation: EAW63356.1.
BC146774 mRNA. Translation: AAI46775.1. Different initiation.
AY181242 mRNA. Translation: AAO27354.1. Different initiation.
AK074099 mRNA. Translation: BAB84925.2.
AL110244 mRNA. Translation: CAB53694.1.
AL832524 mRNA. Translation: CAD38629.1.
CCDSiCCDS6097.2. [Q96PE1-1]
PIRiT14774.
RefSeqiNP_116166.9. NM_032777.9. [Q96PE1-1]
UniGeneiHs.274136.

3D structure databases

ProteinModelPortaliQ96PE1.
SMRiQ96PE1. Positions 38-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117450. 2 interactions.
IntActiQ96PE1. 1 interaction.
STRINGi9606.ENSP00000406367.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ96PE1.
PhosphoSiteiQ96PE1.

Polymorphism and mutation databases

BioMutaiGPR124.
DMDMi221222450.

Proteomic databases

PaxDbiQ96PE1.
PeptideAtlasiQ96PE1.
PRIDEiQ96PE1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315215; ENSP00000323508; ENSG00000020181. [Q96PE1-2]
ENST00000412232; ENSP00000406367; ENSG00000020181. [Q96PE1-1]
GeneIDi25960.
KEGGihsa:25960.
UCSCiuc003xkj.4. human. [Q96PE1-1]

Organism-specific databases

CTDi25960.
GeneCardsiADGRA2.
HGNCiHGNC:17849. ADGRA2.
HPAiHPA012393.
MIMi606823. gene.
neXtProtiNX_Q96PE1.
PharmGKBiPA134869404.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129728.
HOVERGENiHBG051777.
InParanoidiQ96PE1.
KOiK08461.
OMAiRTLCAYP.
OrthoDBiEOG7RV9F8.
PhylomeDBiQ96PE1.
TreeFamiTF331206.

Miscellaneous databases

ChiTaRSiGPR124. human.
GeneWikiiGPR124.
GenomeRNAii25960.
PROiQ96PE1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96PE1.
CleanExiHS_GPR124.
ExpressionAtlasiQ96PE1. baseline and differential.
GenevisibleiQ96PE1. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cell surface tumor endothelial markers are conserved in mice and humans."
    Carson-Walter E.B., Watkins D.N., Nanda A., Vogelstein B., Kinzler K.W., St Croix B.
    Cancer Res. 61:6649-6655(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Embryo.
  5. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  8. "There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini."
    Fredriksson R., Gloriam D.E.I., Hoeglund P.J., Lagerstroem M.C., Schioeth H.B.
    Biochem. Biophys. Res. Commun. 301:725-734(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-1338 (ISOFORM 1).
  9. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 29-1338 (ISOFORM 1).
    Tissue: Spleen.
  10. "Thrombin-induced shedding of tumour endothelial marker 5 and exposure of its RGD motif are regulated by cell-surface protein disulfide-isomerase."
    Vallon M., Aubele P., Janssen K.P., Essler M.
    Biochem. J. 441:937-944(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 370-374 AND 399-403, SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE, PRESENCE OF DISULFIDE BONDS, TOPOLOGY, MUTAGENESIS OF ARG-369 AND ARG-398.
  11. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 850-1338.
    Tissue: Amygdala and Testis.
  12. "Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein."
    Yamamoto Y., Irie K., Asada M., Mino A., Mandai K., Takai Y.
    Oncogene 23:3889-3897(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH DLG1, MOTIF PDZ-BINDING, MUTAGENESIS OF 1335-GLU--VAL-1338.
  13. "Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans."
    Vallon M., Essler M.
    J. Biol. Chem. 281:34179-34188(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ITGAV AND ITGB3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PROTEOLYTIC CLEAVAGE, TOPOLOGY, MOTIF RGD.
  14. "GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier."
    Cullen M., Elzarrad M.K., Seaman S., Zudaire E., Stevens J., Yang M.Y., Li X., Chaudhary A., Xu L., Hilton M.B., Logsdon D., Hsiao E., Stein E.V., Cuttitta F., Haines D.C., Nagashima K., Tessarollo L., St Croix B.
    Proc. Natl. Acad. Sci. U.S.A. 108:5759-5764(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, TOPOLOGY.
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  16. "Exome sequencing identifies de novo gain of function missense mutation in KCND2 in identical twins with autism and seizures that slows potassium channel inactivation."
    Lee H., Lin M.C., Kornblum H.I., Papazian D.M., Nelson S.F.
    Hum. Mol. Genet. 23:3481-3489(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LEU-29.
  17. Cited for: VARIANT LYS-375.

Entry informationi

Entry nameiAGRA2_HUMAN
AccessioniPrimary (citable) accession number: Q96PE1
Secondary accession number(s): A6H8W3
, D3DSW4, Q8N3R1, Q8TEM3, Q96KB2, Q9P1Z7, Q9UFY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 20, 2009
Last modified: July 6, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.