SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q96PD7

- DGAT2_HUMAN

UniProt

Q96PD7 - DGAT2_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diacylglycerol O-acyltransferase 2
Gene
DGAT2, HMFN1045, UNQ738/PRO1433
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides By similarity. Functions also as an acyl-CoA retinol acyltransferase (ARAT).

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

Enzyme regulationi

Inhibited by niacin By similarity.

Pathwayi

GO - Molecular functioni

  1. 2-acylglycerol O-acyltransferase activity Source: Ensembl
  2. diacylglycerol O-acyltransferase activity Source: UniProtKB
  3. protein homodimerization activity Source: BHF-UCL
  4. retinol O-fatty-acyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. acylglycerol acyl-chain remodeling Source: Reactome
  2. cellular lipid metabolic process Source: Reactome
  3. cellular response to oleic acid Source: BHF-UCL
  4. cellular triglyceride homeostasis Source: BHF-UCL
  5. cholesterol homeostasis Source: BHF-UCL
  6. diacylglycerol metabolic process Source: BHF-UCL
  7. fat pad development Source: BHF-UCL
  8. fatty acid homeostasis Source: BHF-UCL
  9. glycerol metabolic process Source: UniProtKB-KW
  10. glycerophospholipid biosynthetic process Source: Reactome
  11. lipid storage Source: BHF-UCL
  12. long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
  13. low-density lipoprotein particle clearance Source: BHF-UCL
  14. phospholipid metabolic process Source: Reactome
  15. regulation of plasma lipoprotein particle levels Source: BHF-UCL
  16. small molecule metabolic process Source: Reactome
  17. triglyceride biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.20. 2681.
ReactomeiREACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.
SABIO-RKQ96PD7.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase 2
Gene namesi
Name:DGAT2
ORF Names:HMFN1045, UNQ738/PRO1433
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:16940. DGAT2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet By similarity 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei70 – 8819Helical; Reviewed prediction
Add
BLAST
Topological domaini89 – 924Lumenal Reviewed prediction
Transmembranei93 – 11220Helical; Reviewed prediction
Add
BLAST
Topological domaini113 – 388276Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: BHF-UCL
  2. endoplasmic reticulum membrane Source: Reactome
  3. integral component of endoplasmic reticulum membrane Source: BHF-UCL
  4. integral component of membrane Source: BHF-UCL
  5. lipid particle Source: UniProtKB-SubCell
  6. mitochondrion Source: Ensembl
  7. perinuclear region of cytoplasm Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27304.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Diacylglycerol O-acyltransferase 2
PRO_0000249045Add
BLAST

Proteomic databases

PaxDbiQ96PD7.
PRIDEiQ96PD7.

PTM databases

PhosphoSiteiQ96PD7.

Expressioni

Tissue specificityi

Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.2 Publications

Gene expression databases

BgeeiQ96PD7.
CleanExiHS_DGAT2.
GenevestigatoriQ96PD7.

Organism-specific databases

HPAiHPA013351.

Interactioni

Subunit structurei

Forms multimeric complexes consisting of several DGAT2 subunits By similarity.

Protein-protein interaction databases

IntActiQ96PD7. 2 interactions.
STRINGi9606.ENSP00000228027.

Structurei

3D structure databases

ProteinModelPortaliQ96PD7.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG258143.
HOVERGENiHBG065791.
InParanoidiQ96PD7.
KOiK11160.
OMAiLMYTFCT.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ96PD7.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96PD7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKTLIAAYSG VLRGERQAEA DRSQRSHGGP ALSREGSGRW GTGSSILSAL    50
QDLFSVTWLN RSKVEKQLQV ISVLQWVLSF LVLGVACSAI LMYIFCTDCW 100
LIAVLYFTWL VFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL 150
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR 200
MPVLREYLMS GGICPVSRDT IDYLLSKNGS GNAIIIVVGG AAESLSSMPG 250
KNAVTLRNRK GFVKLALRHG ADLVPIYSFG ENEVYKQVIF EEGSWGRWVQ 300
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITIPKLEHP 350
TQQDIDLYHT MYMEALVKLF DKHKTKFGLP ETEVLEVN 388
Length:388
Mass (Da):43,831
Last modified:March 1, 2002 - v2
Checksum:i39EE7783A3F06593
GO
Isoform 2 (identifier: Q96PD7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-83: Missing.

Show »
Length:345
Mass (Da):39,044
Checksum:iCDDCB976A9D4E539
GO

Sequence cautioni

The sequence BAD38635.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAD38961.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti317 – 3171R → G.
Corresponds to variant rs34421064 [ dbSNP | Ensembl ].
VAR_033864
Natural varianti361 – 3611M → I.
Corresponds to variant rs34113941 [ dbSNP | Ensembl ].
VAR_033865

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei41 – 8343Missing in isoform 2.
VSP_020356Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti295 – 2951W → C in AAV35727. 1 Publication
Sequence conflicti304 – 3041Q → H in AAV35727. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF384161 mRNA. Translation: AAK84176.2.
AB048286 mRNA. Translation: BAB40641.2.
AY358532 mRNA. Translation: AAQ88896.1.
AL834287 mRNA. Translation: CAD38961.1. Different initiation.
CR749377 mRNA. Translation: CAH18230.1.
AP001922 Genomic DNA. No translation available.
BC015234 mRNA. Translation: AAH15234.1.
AY780647 mRNA. Translation: AAV35727.1.
AB073384 mRNA. Translation: BAD38635.1. Different initiation.
CCDSiCCDS31642.1. [Q96PD7-1]
CCDS58162.1. [Q96PD7-2]
RefSeqiNP_001240820.1. NM_001253891.1. [Q96PD7-2]
NP_115953.2. NM_032564.4. [Q96PD7-1]
UniGeneiHs.129798.

Genome annotation databases

EnsembliENST00000228027; ENSP00000228027; ENSG00000062282. [Q96PD7-1]
ENST00000376262; ENSP00000365438; ENSG00000062282. [Q96PD7-2]
GeneIDi84649.
KEGGihsa:84649.
UCSCiuc001oxa.3. human. [Q96PD7-1]
uc001oxb.3. human. [Q96PD7-2]

Polymorphism databases

DMDMi74732654.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF384161 mRNA. Translation: AAK84176.2 .
AB048286 mRNA. Translation: BAB40641.2 .
AY358532 mRNA. Translation: AAQ88896.1 .
AL834287 mRNA. Translation: CAD38961.1 . Different initiation.
CR749377 mRNA. Translation: CAH18230.1 .
AP001922 Genomic DNA. No translation available.
BC015234 mRNA. Translation: AAH15234.1 .
AY780647 mRNA. Translation: AAV35727.1 .
AB073384 mRNA. Translation: BAD38635.1 . Different initiation.
CCDSi CCDS31642.1. [Q96PD7-1 ]
CCDS58162.1. [Q96PD7-2 ]
RefSeqi NP_001240820.1. NM_001253891.1. [Q96PD7-2 ]
NP_115953.2. NM_032564.4. [Q96PD7-1 ]
UniGenei Hs.129798.

3D structure databases

ProteinModelPortali Q96PD7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q96PD7. 2 interactions.
STRINGi 9606.ENSP00000228027.

Chemistry

BindingDBi Q96PD7.
ChEMBLi CHEMBL5853.

PTM databases

PhosphoSitei Q96PD7.

Polymorphism databases

DMDMi 74732654.

Proteomic databases

PaxDbi Q96PD7.
PRIDEi Q96PD7.

Protocols and materials databases

DNASUi 84649.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000228027 ; ENSP00000228027 ; ENSG00000062282 . [Q96PD7-1 ]
ENST00000376262 ; ENSP00000365438 ; ENSG00000062282 . [Q96PD7-2 ]
GeneIDi 84649.
KEGGi hsa:84649.
UCSCi uc001oxa.3. human. [Q96PD7-1 ]
uc001oxb.3. human. [Q96PD7-2 ]

Organism-specific databases

CTDi 84649.
GeneCardsi GC11P075479.
HGNCi HGNC:16940. DGAT2.
HPAi HPA013351.
MIMi 606983. gene.
neXtProti NX_Q96PD7.
PharmGKBi PA27304.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG258143.
HOVERGENi HBG065791.
InParanoidi Q96PD7.
KOi K11160.
OMAi LMYTFCT.
OrthoDBi EOG7KH9KB.
PhylomeDBi Q96PD7.
TreeFami TF314707.

Enzyme and pathway databases

UniPathwayi UPA00282 .
BRENDAi 2.3.1.20. 2681.
Reactomei REACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.
SABIO-RK Q96PD7.

Miscellaneous databases

ChiTaRSi DGAT2. human.
GenomeRNAii 84649.
NextBioi 74602.
PROi Q96PD7.
SOURCEi Search...

Gene expression databases

Bgeei Q96PD7.
CleanExi HS_DGAT2.
Genevestigatori Q96PD7.

Family and domain databases

InterProi IPR007130. DAGAT.
[Graphical view ]
PANTHERi PTHR12317. PTHR12317. 1 hit.
Pfami PF03982. DAGAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members."
    Cases S., Stone S.J., Zhou P., Yen C.-L.E., Tow B., Lardizabal K.D., Voelker T., Farese R.V. Jr.
    J. Biol. Chem. 276:38870-38876(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice."
    Wakimoto K., Chiba H., Michibata H., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
    Biochem. Biophys. Res. Commun. 310:296-302(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph node and Uterus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  7. "Evaluation of gene structure of human DGAT2 gene."
    Reichwald K., Petz U., Platzer M.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-341 (ISOFORM 2).
    Tissue: Adipocyte.
  8. "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
    Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
    Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-388.
    Tissue: Hepatoblastoma.
  9. "Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1."
    Orland M.D., Anwar K., Cromley D., Chu C.H., Chen L., Billheimer J.T., Hussain M.M., Cheng D.
    Biochim. Biophys. Acta 1737:76-82(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.

Entry informationi

Entry nameiDGAT2_HUMAN
AccessioniPrimary (citable) accession number: Q96PD7
Secondary accession number(s): A6ND76
, Q5U810, Q68CL3, Q68DJ0, Q8NDB7, Q96BS0, Q9BYE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi