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Protein

Diacylglycerol O-acyltransferase 2

Gene

DGAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

Enzyme regulationi

Inhibited by niacin.By similarity

Pathway: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.20. 2681.
ReactomeiREACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.
SABIO-RKQ96PD7.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase 2
Gene namesi
Name:DGAT2
ORF Names:HMFN1045, UNQ738/PRO1433
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:16940. DGAT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei70 – 8819HelicalSequence AnalysisAdd
BLAST
Topological domaini89 – 924LumenalSequence Analysis
Transmembranei93 – 11220HelicalSequence AnalysisAdd
BLAST
Topological domaini113 – 388276CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27304.

Polymorphism and mutation databases

BioMutaiDGAT2.
DMDMi74732654.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Diacylglycerol O-acyltransferase 2PRO_0000249045Add
BLAST

Proteomic databases

PaxDbiQ96PD7.
PRIDEiQ96PD7.

PTM databases

PhosphoSiteiQ96PD7.

Expressioni

Tissue specificityi

Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.2 Publications

Gene expression databases

BgeeiQ96PD7.
CleanExiHS_DGAT2.
ExpressionAtlasiQ96PD7. baseline and differential.
GenevisibleiQ96PD7. HS.

Organism-specific databases

HPAiHPA013351.

Interactioni

Subunit structurei

Forms multimeric complexes consisting of several DGAT2 subunits.By similarity

Protein-protein interaction databases

BioGridi124172. 3 interactions.
IntActiQ96PD7. 2 interactions.
STRINGi9606.ENSP00000228027.

Structurei

3D structure databases

ProteinModelPortaliQ96PD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG258143.
GeneTreeiENSGT00750000117391.
HOVERGENiHBG065791.
InParanoidiQ96PD7.
KOiK11160.
OMAiAILMYTF.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ96PD7.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96PD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTLIAAYSG VLRGERQAEA DRSQRSHGGP ALSREGSGRW GTGSSILSAL
60 70 80 90 100
QDLFSVTWLN RSKVEKQLQV ISVLQWVLSF LVLGVACSAI LMYIFCTDCW
110 120 130 140 150
LIAVLYFTWL VFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL
160 170 180 190 200
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR
210 220 230 240 250
MPVLREYLMS GGICPVSRDT IDYLLSKNGS GNAIIIVVGG AAESLSSMPG
260 270 280 290 300
KNAVTLRNRK GFVKLALRHG ADLVPIYSFG ENEVYKQVIF EEGSWGRWVQ
310 320 330 340 350
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITIPKLEHP
360 370 380
TQQDIDLYHT MYMEALVKLF DKHKTKFGLP ETEVLEVN
Length:388
Mass (Da):43,831
Last modified:March 1, 2002 - v2
Checksum:i39EE7783A3F06593
GO
Isoform 2 (identifier: Q96PD7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-83: Missing.

Show »
Length:345
Mass (Da):39,044
Checksum:iCDDCB976A9D4E539
GO

Sequence cautioni

The sequence BAD38635.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAD38961.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti295 – 2951W → C in AAV35727 (Ref. 7) Curated
Sequence conflicti304 – 3041Q → H in AAV35727 (Ref. 7) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti317 – 3171R → G.
Corresponds to variant rs34421064 [ dbSNP | Ensembl ].
VAR_033864
Natural varianti361 – 3611M → I.
Corresponds to variant rs34113941 [ dbSNP | Ensembl ].
VAR_033865

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei41 – 8343Missing in isoform 2. 1 PublicationVSP_020356Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384161 mRNA. Translation: AAK84176.2.
AB048286 mRNA. Translation: BAB40641.2.
AY358532 mRNA. Translation: AAQ88896.1.
AL834287 mRNA. Translation: CAD38961.1. Different initiation.
CR749377 mRNA. Translation: CAH18230.1.
AP001922 Genomic DNA. No translation available.
BC015234 mRNA. Translation: AAH15234.1.
AY780647 mRNA. Translation: AAV35727.1.
AB073384 mRNA. Translation: BAD38635.1. Different initiation.
CCDSiCCDS31642.1. [Q96PD7-1]
CCDS58162.1. [Q96PD7-2]
RefSeqiNP_001240820.1. NM_001253891.1. [Q96PD7-2]
NP_115953.2. NM_032564.4. [Q96PD7-1]
UniGeneiHs.129798.

Genome annotation databases

EnsembliENST00000228027; ENSP00000228027; ENSG00000062282. [Q96PD7-1]
ENST00000376262; ENSP00000365438; ENSG00000062282. [Q96PD7-2]
GeneIDi84649.
KEGGihsa:84649.
UCSCiuc001oxa.3. human. [Q96PD7-1]
uc001oxb.3. human. [Q96PD7-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384161 mRNA. Translation: AAK84176.2.
AB048286 mRNA. Translation: BAB40641.2.
AY358532 mRNA. Translation: AAQ88896.1.
AL834287 mRNA. Translation: CAD38961.1. Different initiation.
CR749377 mRNA. Translation: CAH18230.1.
AP001922 Genomic DNA. No translation available.
BC015234 mRNA. Translation: AAH15234.1.
AY780647 mRNA. Translation: AAV35727.1.
AB073384 mRNA. Translation: BAD38635.1. Different initiation.
CCDSiCCDS31642.1. [Q96PD7-1]
CCDS58162.1. [Q96PD7-2]
RefSeqiNP_001240820.1. NM_001253891.1. [Q96PD7-2]
NP_115953.2. NM_032564.4. [Q96PD7-1]
UniGeneiHs.129798.

3D structure databases

ProteinModelPortaliQ96PD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124172. 3 interactions.
IntActiQ96PD7. 2 interactions.
STRINGi9606.ENSP00000228027.

Chemistry

BindingDBiQ96PD7.
ChEMBLiCHEMBL5853.

PTM databases

PhosphoSiteiQ96PD7.

Polymorphism and mutation databases

BioMutaiDGAT2.
DMDMi74732654.

Proteomic databases

PaxDbiQ96PD7.
PRIDEiQ96PD7.

Protocols and materials databases

DNASUi84649.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228027; ENSP00000228027; ENSG00000062282. [Q96PD7-1]
ENST00000376262; ENSP00000365438; ENSG00000062282. [Q96PD7-2]
GeneIDi84649.
KEGGihsa:84649.
UCSCiuc001oxa.3. human. [Q96PD7-1]
uc001oxb.3. human. [Q96PD7-2]

Organism-specific databases

CTDi84649.
GeneCardsiGC11P075479.
HGNCiHGNC:16940. DGAT2.
HPAiHPA013351.
MIMi606983. gene.
neXtProtiNX_Q96PD7.
PharmGKBiPA27304.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG258143.
GeneTreeiENSGT00750000117391.
HOVERGENiHBG065791.
InParanoidiQ96PD7.
KOiK11160.
OMAiAILMYTF.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ96PD7.
TreeFamiTF314707.

Enzyme and pathway databases

UniPathwayiUPA00282.
BRENDAi2.3.1.20. 2681.
ReactomeiREACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.
SABIO-RKQ96PD7.

Miscellaneous databases

ChiTaRSiDGAT2. human.
GenomeRNAii84649.
NextBioi74602.
PROiQ96PD7.
SOURCEiSearch...

Gene expression databases

BgeeiQ96PD7.
CleanExiHS_DGAT2.
ExpressionAtlasiQ96PD7. baseline and differential.
GenevisibleiQ96PD7. HS.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members."
    Cases S., Stone S.J., Zhou P., Yen C.-L.E., Tow B., Lardizabal K.D., Voelker T., Farese R.V. Jr.
    J. Biol. Chem. 276:38870-38876(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice."
    Wakimoto K., Chiba H., Michibata H., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
    Biochem. Biophys. Res. Commun. 310:296-302(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph node and Uterus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  7. "Evaluation of gene structure of human DGAT2 gene."
    Reichwald K., Petz U., Platzer M.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-341 (ISOFORM 2).
    Tissue: Adipocyte.
  8. "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
    Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
    Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-388.
    Tissue: Hepatoblastoma.
  9. "Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1."
    Orland M.D., Anwar K., Cromley D., Chu C.H., Chen L., Billheimer J.T., Hussain M.M., Cheng D.
    Biochim. Biophys. Acta 1737:76-82(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.

Entry informationi

Entry nameiDGAT2_HUMAN
AccessioniPrimary (citable) accession number: Q96PD7
Secondary accession number(s): A6ND76
, Q5U810, Q68CL3, Q68DJ0, Q8NDB7, Q96BS0, Q9BYE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.