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Protein

N-acetylmuramoyl-L-alanine amidase

Gene

PGLYRP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi411ZincBy similarity1
Metal bindingi447ZincBy similarity1
Metal bindingi522ZincBy similarity1
Metal bindingi530ZincBy similarity1

GO - Molecular functioni

  • N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB
  • peptidoglycan binding Source: UniProtKB
  • peptidoglycan receptor activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS08567-MONOMER.
BRENDAi3.5.1.28. 2681.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Peptidoglycan recognition protein 2
Peptidoglycan recognition protein long
Short name:
PGRP-L
Gene namesi
Name:PGLYRP2
Synonyms:PGLYRPL, PGRPL
ORF Names:UNQ3103/PRO10102
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:30013. PGLYRP2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • intracellular Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi411H → A: No effect on amidase activity. 1 Publication1
Mutagenesisi419C → A: Abolishes amidase activity. 1 Publication1
Mutagenesisi436H → A: No effect on amidase activity. 1 Publication1
Mutagenesisi442W → A: Reduced amidase activity. 1 Publication1
Mutagenesisi447Y → A: Abolishes amidase activity. 1
Mutagenesisi530C → S: Abolishes amidase activity. 1 Publication1

Organism-specific databases

DisGeNETi114770.
OpenTargetsiENSG00000161031.
PharmGKBiPA134929965.

Polymorphism and mutation databases

BioMutaiPGLYRP2.
DMDMi38258222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000002392022 – 576N-acetylmuramoyl-L-alanine amidaseAdd BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi77N-linked (GlcNAc...)1 Publication1
Modified residuei239Phosphoserine1 Publication1
Modified residuei274Deamidated asparagine1 Publication1
Modified residuei322Deamidated asparagine1 Publication1
Glycosylationi367N-linked (GlcNAc...)2 Publications1
Disulfide bondi419 ↔ 4251 Publication
Glycosylationi485N-linked (GlcNAc...)3 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ96PD5.
PeptideAtlasiQ96PD5.
PRIDEiQ96PD5.

PTM databases

iPTMnetiQ96PD5.
PhosphoSitePlusiQ96PD5.

Expressioni

Tissue specificityi

Strongly expressed in liver and fetal liver, and secreted into serum. Expressed to a much lesser extent in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach and testis. Isoform 2 is not detected in the liver or serum.2 Publications

Gene expression databases

BgeeiENSG00000161031.
CleanExiHS_PGLYRP2.
ExpressionAtlasiQ96PD5. baseline and differential.
GenevisibleiQ96PD5. HS.

Organism-specific databases

HPAiCAB033468.
HPA043568.
HPA046311.

Interactioni

Protein-protein interaction databases

IntActiQ96PD5. 1 interactor.
STRINGi9606.ENSP00000345968.

Structurei

3D structure databases

ProteinModelPortaliQ96PD5.
SMRiQ96PD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ96PD5.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ96PD5.
TreeFamiTF323898.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 2 hits.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96PD5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQGVLWILL GLLLWSDPGT ASLPLLMDSV IQALAELEQK VPAAKTRHTA
60 70 80 90 100
SAWLMSAPNS GPHNRLYHFL LGAWSLNATE LDPCPLSPEL LGLTKEVARH
110 120 130 140 150
DVREGKEYGV VLAPDGSTVA VEPLLAGLEA GLQGRRVINL PLDSMAAPWE
160 170 180 190 200
TGDTFPDVVA IAPDVRATSS PGLRDGSPDV TTADIGANTP DATKGCPDVQ
210 220 230 240 250
ASLPDAKAKS PPTMVDSLLA VTLAGNLGLT FLRGSQTQSH PDLGTEGCWD
260 270 280 290 300
QLSAPRTFTL LDPKASLLTM AFLNGALDGV ILGDYLSRTP EPRPSLSHLL
310 320 330 340 350
SQYYGAGVAR DPGFRSNFRR QNGAALTSAS ILAQQVWGTL VLLQRLEPVH
360 370 380 390 400
LQLQCMSQEQ LAQVAANATK EFTEAFLGCP AIHPRCRWGA APYRGRPKLL
410 420 430 440 450
QLPLGFLYVH HTYVPAPPCT DFTRCAANMR SMQRYHQDTQ GWGDIGYSFV
460 470 480 490 500
VGSDGYVYEG RGWHWVGAHT LGHNSRGFGV AIVGNYTAAL PTEAALRTVR
510 520 530 540 550
DTLPSCAVRA GLLRPDYALL GHRQLVRTDC PGDALFDLLR TWPHFTATVK
560 570
PRPARSVSKR SRREPPPRTL PATDLQ
Note: Major isoform.
Length:576
Mass (Da):62,217
Last modified:December 1, 2001 - v1
Checksum:i73EA8713DC54F85A
GO
Isoform 2 (identifier: Q96PD5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     548-576: TVKPRPARSVSKRSRREPPPRTLPATDLQ → VSLRSLHYTA...TQPACPFPSS

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:634
Mass (Da):68,000
Checksum:i93FFCAFB353E47A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124L → P in AAI44239 (PubMed:15489334).Curated1
Sequence conflicti448S → G in BAB71034 (PubMed:14702039).Curated1
Sequence conflicti562R → G in BAF82104 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05049846T → A.1 PublicationCorresponds to variant rs3813135dbSNPEnsembl.1
Natural variantiVAR_05049999R → Q.1 PublicationCorresponds to variant rs733731dbSNPEnsembl.1
Natural variantiVAR_050500257T → N.Corresponds to variant rs28404490dbSNPEnsembl.1
Natural variantiVAR_050501270M → K.1 PublicationCorresponds to variant rs892145dbSNPEnsembl.1
Natural variantiVAR_055231394R → Q.2 PublicationsCorresponds to variant rs34440547dbSNPEnsembl.1
Natural variantiVAR_050502476R → W.Corresponds to variant rs2304200dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008964548 – 576TVKPR…ATDLQ → VSLRSLHYTARRPSVYTSST RPLPPACNSCARTASARPPT SRRHVYSGNLGPAFAGHSAG NIPDPVTSAYAASAQPQTQP ACPFPSS in isoform 2. 2 PublicationsAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384856 mRNA. Translation: AAL05629.1.
AB073610 mRNA. Translation: BAD38647.1.
AY358156 mRNA. Translation: AAQ88523.1.
AK055882 mRNA. Translation: BAB71034.1.
AK289415 mRNA. Translation: BAF82104.1.
AK292292 mRNA. Translation: BAF84981.1.
CH471106 Genomic DNA. Translation: EAW84482.1.
CH471106 Genomic DNA. Translation: EAW84483.1.
BC136551 mRNA. Translation: AAI36552.1.
BC136552 mRNA. Translation: AAI36553.1.
BC144238 mRNA. Translation: AAI44239.1.
CCDSiCCDS12330.2. [Q96PD5-1]
RefSeqiNP_443122.3. NM_052890.3. [Q96PD5-1]
XP_006722696.1. XM_006722633.3. [Q96PD5-2]
UniGeneiHs.282244.

Genome annotation databases

EnsembliENST00000292609; ENSP00000292609; ENSG00000161031. [Q96PD5-2]
ENST00000340880; ENSP00000345968; ENSG00000161031. [Q96PD5-1]
GeneIDi114770.
KEGGihsa:114770.
UCSCiuc002nbf.5. human. [Q96PD5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384856 mRNA. Translation: AAL05629.1.
AB073610 mRNA. Translation: BAD38647.1.
AY358156 mRNA. Translation: AAQ88523.1.
AK055882 mRNA. Translation: BAB71034.1.
AK289415 mRNA. Translation: BAF82104.1.
AK292292 mRNA. Translation: BAF84981.1.
CH471106 Genomic DNA. Translation: EAW84482.1.
CH471106 Genomic DNA. Translation: EAW84483.1.
BC136551 mRNA. Translation: AAI36552.1.
BC136552 mRNA. Translation: AAI36553.1.
BC144238 mRNA. Translation: AAI44239.1.
CCDSiCCDS12330.2. [Q96PD5-1]
RefSeqiNP_443122.3. NM_052890.3. [Q96PD5-1]
XP_006722696.1. XM_006722633.3. [Q96PD5-2]
UniGeneiHs.282244.

3D structure databases

ProteinModelPortaliQ96PD5.
SMRiQ96PD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ96PD5. 1 interactor.
STRINGi9606.ENSP00000345968.

PTM databases

iPTMnetiQ96PD5.
PhosphoSitePlusiQ96PD5.

Polymorphism and mutation databases

BioMutaiPGLYRP2.
DMDMi38258222.

Proteomic databases

PaxDbiQ96PD5.
PeptideAtlasiQ96PD5.
PRIDEiQ96PD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292609; ENSP00000292609; ENSG00000161031. [Q96PD5-2]
ENST00000340880; ENSP00000345968; ENSG00000161031. [Q96PD5-1]
GeneIDi114770.
KEGGihsa:114770.
UCSCiuc002nbf.5. human. [Q96PD5-1]

Organism-specific databases

CTDi114770.
DisGeNETi114770.
GeneCardsiPGLYRP2.
HGNCiHGNC:30013. PGLYRP2.
HPAiCAB033468.
HPA043568.
HPA046311.
MIMi608199. gene.
neXtProtiNX_Q96PD5.
OpenTargetsiENSG00000161031.
PharmGKBiPA134929965.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ96PD5.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ96PD5.
TreeFamiTF323898.

Enzyme and pathway databases

BioCyciZFISH:HS08567-MONOMER.
BRENDAi3.5.1.28. 2681.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

GeneWikiiPGLYRP2.
GenomeRNAii114770.
PROiQ96PD5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161031.
CleanExiHS_PGLYRP2.
ExpressionAtlasiQ96PD5. baseline and differential.
GenevisibleiQ96PD5. HS.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 2 hits.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGRP2_HUMAN
AccessioniPrimary (citable) accession number: Q96PD5
Secondary accession number(s): A8K050
, A8K8C7, B2RMZ2, B7ZM33, Q68CK1, Q96N74, Q9UC60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.