Reviewed, UniProtKB/Swiss-Prot Q96PD4 (IL17F_HUMAN)

Names and origin

Protein names

Recommended name:
    Interleukin-17F
      Short name=IL-17F
Alternative name(s):
    Interleukin-24
      Short name=IL-24
    Cytokine ML-1

Gene names

Name:	IL17F
Synonyms:	IL24

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Secreted.
Tissue specificity	Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
Sequence similarities	Belongs to the IL-17 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cartilage development Ref.6 Inferred from direct assay. Source: UniProtKB inflammatory response Inferred from electronic annotation. Source: InterPro lymphotoxin A biosynthetic process Ref.1 Inferred from direct assay. Source: UniProtKB negative regulation of angiogenesis Ref.1 Inferred from direct assay. Source: UniProtKB proteoglycan metabolic process Ref.6 Inferred from direct assay. Source: UniProtKB regulation of granulocyte macrophage colony-stimulating factor biosynthetic process Ref.6 Inferred from direct assay. Source: UniProtKB regulation of interleukin-2 biosynthetic process Ref.1 Inferred from direct assay. Source: UniProtKB regulation of interleukin-6 biosynthetic process Ref.6 Inferred from direct assay. Source: UniProtKB regulation of interleukin-8 biosynthetic process Ref.6 Inferred from direct assay. Source: UniProtKB regulation of transforming growth factor beta receptor signaling pathway Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Ref.1 Inferred from direct assay. Source: UniProtKB cytokine binding Ref.6 Inferred from direct assay. Source: UniProtKB protein homodimerization activity Ref.6 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96PD4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96PD4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 30

30

Chain

31 – 163

133

Interleukin-17F

PRO_0000015432

Amino acid modifications

Glycosylation

83

1

N-linked (GlcNAc...)

Disulfide bond

47

Interchain (with C-137)

Disulfide bond

102 ↔ 152

Disulfide bond

107 ↔ 154

Disulfide bond

137

Interchain (with C-47)

Natural variations

Alternative sequence

1 – 10

10

Missing in isoform 2.

VSP_037500

Experimental info

Sequence conflict

161

1

H → R in AAH70124. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

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.

.

.

.

.

.

.

.

.

.

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163

Helix Strand Turn

Details...

Helix

39 – 41

3

Beta strand

51 – 62

12

Turn

63 – 65

3

Helix

74 – 77

4

Beta strand

78 – 80

3

Beta strand

82 – 88

7

Beta strand

92 – 103

12

Beta strand

105 – 108

4

Beta strand

110 – 112

3

Beta strand

118 – 135

18

Helix

136 – 138

3

Beta strand

140 – 155

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: E5287737C9E7BD46	FASTA	163	18,045
	10 20 30 40 50 60 MTVKTLHGPA MVKYLLLSIL GLAFLSEAAA RKIPKVGHTF FQKPESCPPV PGGSMKLDIG 70 80 90 100 110 120 IINENQRVSM SRNIESRSTS PWNYTVTWDP NRYPSEVVQA QCRNLGCINA QGKEDISMNS 130 140 150 160 VPIQQETLVV RRKHQGCSVS FQLEKVLVTV GCTCVTPVIH HVQ
	Isoform 2. Checksum: BB489BED883D57CE	FASTA	153	17,009
	10 20 30 40 50 60 MVKYLLLSIL GLAFLSEAAA RKIPKVGHTF FQKPESCPPV PGGSMKLDIG IINENQRVSM 70 80 90 100 110 120 SRNIESRSTS PWNYTVTWDP NRYPSEVVQA QCRNLGCINA QGKEDISMNS VPIQQETLVV 130 140 150 RRKHQGCSVS FQLEKVLVTV GCTCVTPVIH HVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production." Starnes T., Robertson M.J., Sledge G., Kelich S., Nakshatri H., Broxmeyer H.E., Hromas R. J. Immunol. 167:4137-4140(2001) [PubMed: 11591732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Blood.
[4]	"Identification of a novel cytokine, ML-1, and its expression in subjects with asthma." Kawaguchi M., Onuchic L.F., Li X.-D., Essayan D.M., Schroeder J., Xiao H.-Q., Liu M.C., Krishnaswamy G., Germino G., Huang S.-K. J. Immunol. 167:4430-4435(2001) [PubMed: 11591768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-163 (ISOFORM 2).
[5]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-45.
[6]	"IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding." Hymowitz S.G., Filvaroff E.H., Yin J.P., Lee J., Cai L., Risser P., Maruoka M., Mao W., Foster J., Kelley R.F., Pan G., Gurney A.L., de Vos A.M., Starovasnik M.A. EMBO J. 20:5332-5341(2001) [PubMed: 11574464] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS), CHARACTERIZATION.
+	Additional computationally mapped references.

Web resources

Wikipedia

Interleukin-17 entry

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF384857 mRNA. Translation: AAK83350.1.
AL034343 Genomic DNA. Translation: CAD92646.1.
BC070124 mRNA. Translation: AAH70124.1.
AF332389 mRNA. Translation: AAL14427.1. Different initiation.

IPI

IPI00791572.

UniGene

Hs.272295

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JPY	X-ray	2.85	A/B/X/Y	21-153	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q96PD4.

Genome annotation databases

Ensembl

ENSG00000112116. Homo sapiens. [Contig view]

UCSC

uc003pal.1. human.

Organism-specific databases

GeneCards

GC06M052209.

H-InvDB

HIX0032794.

HGNC

HGNC:16404. IL17F.

MIM

606496. gene.

PharmGKB

PA29800.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q96PD4.

Enzyme and pathway databases

Pathway_Interaction_DB

il23pathway. IL23-mediated signaling events.

Gene expression databases

ArrayExpress

Q96PD4.

Bgee

Q96PD4.

CleanEx

HS_IL17F.
HS_IL24.

GermOnline

ENSG00000112116. Homo sapiens.

Family and domain databases

InterPro

IPR010345. IL17.
[Graphical view]

PANTHER

PTHR21295. IL17. 1 hit.

Pfam

PF06083. IL17. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

IL17F_HUMAN

Accession

Primary (citable) accession number: Q96PD4
Secondary accession number(s): Q6NSI0 , Q7Z6P4, Q96PI8, Q9NUE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	June 16, 2009
Last modified:	July 7, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)