Q96PD2 (DCBD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Discoidin, CUB and LCCL domain-containing protein 2

Alternative name(s):
CUB, LCCL and coagulation factor V/VIII-homology domains protein 1
Endothelial and smooth muscle cell-derived neuropilin-like protein

Gene names

Name:	DCBLD2
Synonyms:	CLCP1, ESDN

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type I membrane protein Ref.2.
Tissue specificity	Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Ref.1 Ref.2
Induction	Increased in lung cancers during the process of tumor progression.
Sequence similarities	Contains 1 CUB domain. Contains 1 F5/8 type C domain. Contains 1 LCCL domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro intracellular receptor mediated signaling pathway Non-traceable author statement Ref.1. Source: UniProtKB negative regulation of cell growth Inferred from direct assay Ref.1. Source: UniProtKB wound healing Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	cell surface Inferred from direct assay Ref.1. Source: UniProtKB integral to plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96PD2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96PD2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-574: G → GFYLMVSLACRHNEG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 66	66	Potential
	Chain	67 – 775	709	Discoidin, CUB and LCCL domain-containing protein 2		PRO_0000021078
Regions
	Topological domain	67 – 528	462	Extracellular Potential
	Transmembrane	529 – 549	21	Helical; Potential
	Topological domain	550 – 775	226	Cytoplasmic Potential
	Domain	72 – 186	115	CUB
	Domain	187 – 285	99	LCCL
	Domain	292 – 449	158	F5/8 type C
	Compositional bias	463 – 466	4	Poly-Pro
Amino acid modifications
	Modified residue	606	1	Phosphoserine Ref.8
	Modified residue	715	1	Phosphotyrosine Ref.9
	Modified residue	732	1	Phosphotyrosine Ref.9
	Modified residue	750	1	Phosphotyrosine Ref.6 Ref.7
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	474	1	N-linked (GlcNAc...) Potential
	Glycosylation	516	1	N-linked (GlcNAc...) Potential
	Glycosylation	522	1	N-linked (GlcNAc...) Potential
	Disulfide bond	72 ↔ 99		By similarity
	Disulfide bond	126 ↔ 148		By similarity
	Disulfide bond	215 ↔ 237		By similarity
	Disulfide bond	292 ↔ 449		By similarity
Natural variations
	Alternative sequence	574	1	G → GFYLMVSLACRHNEG in isoform 2.		VSP_010715
	Natural variant	144	1	I → M. Corresponds to variant rs9838238 [ dbSNP | Ensembl ].		VAR_050944
	Natural variant	723	1	D → N. Corresponds to variant rs16840208 [ dbSNP | Ensembl ].		VAR_050945

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3D06F81EF2337010	FASTA	775	85,035
	10 20 30 40 50 60 MASRAVVRAR RCPQCPQVRA AAAAPAWAAL PLSRSLPPCS NSSSFSMPLF LLLLLVLLLL 70 80 90 100 110 120 LEDAGAQQGD GCGHTVLGPE SGTLTSINYP QTYPNSTVCE WEIRVKMGER VRIKFGDFDI 130 140 150 160 170 180 EDSDSCHFNY LRIYNGIGVS RTEIGKYCGL GLQMNHSIES KGNEITLLFM SGIHVSGRGF 190 200 210 220 230 240 LASYSVIDKQ DLITCLDTAS NFLEPEFSKY CPAGCLLPFA EISGTIPHGY RDSSPLCMAG 250 260 270 280 290 300 VHAGVVSNTL GGQISVVISK GIPYYESSLA NNVTSVVGHL STSLFTFKTS GCYGTLGMES 310 320 330 340 350 360 GVIADPQITA SSVLEWTDHT GQENSWKPKK ARLKKPGPPW AAFATDEYQW LQIDLNKEKK 370 380 390 400 410 420 ITGIITTGST MVEHNYYVSA YRILYSDDGQ KWTVYREPGV EQDKIFQGNK DYHQDVRNNF 430 440 450 460 470 480 LPPIIARFIR VNPTQWQQKI AMKMELLGCQ FIPKGRPPKL TQPPPPRNSN DLKNTTAPPK 490 500 510 520 530 540 IAKGRAPKFT QPLQPRSSNE FPAQTEQTTA SPDIRNTTVT PNVTKDVALA AVLVPVLVMV 550 560 570 580 590 600 LTTLILILVC AWHWRNRKKK TEGTYDLPYW DRAGWWKGMK QFLPAKAVDH EETPVRYSSS 610 620 630 640 650 660 EVNHLSPREV TTVLQADSAE YAQPLVGGIV GTLHQRSTFK PEEGKEAGYA DLDPYNSPGQ 670 680 690 700 710 720 EVYHAYAEPL PITGPEYATP IIMDMSGHPT TSVGQPSTST FKATGNQPPP LVGTYNTLLS 730 740 750 760 770 RTDSCSSAQA QYDTPKAGKP GLPAPDELVY QVPQSTQEVS GAGRDGECDV FKEIL
	Isoform 2 [UniParc]. Checksum: D22BB46FD86DD55E	FASTA	789	86,657
	10 20 30 40 50 60 MASRAVVRAR RCPQCPQVRA AAAAPAWAAL PLSRSLPPCS NSSSFSMPLF LLLLLVLLLL 70 80 90 100 110 120 LEDAGAQQGD GCGHTVLGPE SGTLTSINYP QTYPNSTVCE WEIRVKMGER VRIKFGDFDI 130 140 150 160 170 180 EDSDSCHFNY LRIYNGIGVS RTEIGKYCGL GLQMNHSIES KGNEITLLFM SGIHVSGRGF 190 200 210 220 230 240 LASYSVIDKQ DLITCLDTAS NFLEPEFSKY CPAGCLLPFA EISGTIPHGY RDSSPLCMAG 250 260 270 280 290 300 VHAGVVSNTL GGQISVVISK GIPYYESSLA NNVTSVVGHL STSLFTFKTS GCYGTLGMES 310 320 330 340 350 360 GVIADPQITA SSVLEWTDHT GQENSWKPKK ARLKKPGPPW AAFATDEYQW LQIDLNKEKK 370 380 390 400 410 420 ITGIITTGST MVEHNYYVSA YRILYSDDGQ KWTVYREPGV EQDKIFQGNK DYHQDVRNNF 430 440 450 460 470 480 LPPIIARFIR VNPTQWQQKI AMKMELLGCQ FIPKGRPPKL TQPPPPRNSN DLKNTTAPPK 490 500 510 520 530 540 IAKGRAPKFT QPLQPRSSNE FPAQTEQTTA SPDIRNTTVT PNVTKDVALA AVLVPVLVMV 550 560 570 580 590 600 LTTLILILVC AWHWRNRKKK TEGTYDLPYW DRAGFYLMVS LACRHNEGWW KGMKQFLPAK 610 620 630 640 650 660 AVDHEETPVR YSSSEVNHLS PREVTTVLQA DSAEYAQPLV GGIVGTLHQR STFKPEEGKE 670 680 690 700 710 720 AGYADLDPYN SPGQEVYHAY AEPLPITGPE YATPIIMDMS GHPTTSVGQP STSTFKATGN 730 740 750 760 770 780 QPPPLVGTYN TLLSRTDSCS SAQAQYDTPK AGKPGLPAPD ELVYQVPQST QEVSGAGRDG ECDVFKEIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ESDN, a novel neuropilin-like membrane protein cloned from vascular cells with the longest secretory signal sequence among eukaryotes, is up-regulated after vascular injury." Kobuke K., Furukawa Y., Sugai M., Tanigaki K., Ohashi N., Matsumori A., Sasayama S., Honjo T., Tashiro K. J. Biol. Chem. 276:34105-34114(2001) [PubMed: 11447234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Coronary artery.
[2]	"Significant up-regulation of a novel gene, CLCP1, in a highly metastatic lung cancer subline as well as in lung cancers in vivo." Koshikawa K., Osada H., Kozaki K., Konishi H., Masuda A., Tatematsu Y., Mitsudomi T., Nakao A., Takahashi T. Oncogene 21:2822-2828(2002) [PubMed: 11973641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Lung.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 47-775 (ISOFORM 2). Tissue: Prostate.
[6]	"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-750, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[7]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-750, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-606, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-715 AND TYR-732, MASS SPECTROMETRY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF387547 mRNA. Translation: AAL30178.1. AB073146 mRNA. Translation: BAB91138.1. AC106728 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW79845.1. CH471052 Genomic DNA. Translation: EAW79847.1. BC029658 mRNA. Translation: AAH29658.1.
IPI	IPI00419836. IPI00433138.
RefSeq	NP_563615.3. NM_080927.3.
UniGene	Hs.203691.
3D structure databases
ProteinModelPortal	Q96PD2.
SMR	Q96PD2. Positions 14-454.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96PD2.
PTM databases
PhosphoSite	Q96PD2.
Polymorphism databases
DMDM	57013820.
Proteomic databases
PRIDE	Q96PD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000326840; ENSP00000321573; ENSG00000057019. ENST00000326857; ENSP00000321646; ENSG00000057019.
GeneID	131566.
KEGG	hsa:131566.
UCSC	uc003dtd.1. human. uc003dte.1. human.
Organism-specific databases
CTD	131566.
GeneCards	GC03M098514.
H-InvDB	HIX0003494.
HGNC	HGNC:24627. DCBLD2.
HPA	HPA016909.
MIM	608698. gene.
neXtProt	NX_Q96PD2.
PharmGKB	PA134869307.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074267.
HOVERGEN	HBG106294.
OMA	FKTSGCY.
OrthoDB	EOG4KKZ2H.
PhylomeDB	Q96PD2.
Gene expression databases
ArrayExpress	Q96PD2.
Bgee	Q96PD2.
CleanEx	HS_DCBLD2.
Genevestigator	Q96PD2.
GermOnline	ENSG00000057019. Homo sapiens.
Family and domain databases
InterPro	IPR000421. Coagulation_fac_5/8-C_type_dom. IPR000859. CUB. IPR008979. Galactose-bd-like. IPR004043. LCCL. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 1 hit. G3DSA:2.170.130.20. LCCL. 1 hit.
Pfam	PF00431. CUB. 1 hit. PF00754. F5_F8_type_C. 1 hit. PF03815. LCCL. 1 hit. [Graphical view]
SMART	SM00042. CUB. 1 hit. SM00231. FA58C. 1 hit. SM00603. LCCL. 1 hit. [Graphical view]
SUPFAM	SSF49854. CUB. 1 hit. SSF49785. Gal_bind_like. 1 hit. SSF69848. LCCL. 1 hit.
PROSITE	PS01180. CUB. 1 hit. PS01285. FA58C_1. 1 hit. PS01286. FA58C_2. False negative. PS50022. FA58C_3. 1 hit. PS50820. LCCL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	82935.
SOURCE	Search...

Entry information

Entry name

DCBD2_HUMAN

Accession

Primary (citable) accession number: Q96PD2
Secondary accession number(s): B7WNL1 , D3DN41, Q8N6M4, Q8TDX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.