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Q96PC3 (AP1S3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit sigma-3
Alternative name(s):
Adapter-related protein complex 1 subunit sigma-1C
Adaptor protein complex AP-1 subunit sigma-1C
Clathrin assembly protein complex 1 sigma-1C small chain
Golgi adaptor HA1/AP1 adaptin sigma-1C subunit
Sigma 1C subunit of AP-1 clathrin
Sigma-adaptin 1C
Sigma1C-adaptin
Gene names
Name:AP1S3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Subcellular location

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes small subunit family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96PC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96PC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTLA
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 4 (identifier: Q96PC3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → TMEEYMNKPTF
Note: No experimental confirmation available.
Isoform 3 (identifier: Q96PC3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     98-104: VCELDII → TWPFARA
     105-154: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154AP-1 complex subunit sigma-3
PRO_0000193801

Natural variations

Alternative sequence98 – 1047VCELDII → TWPFARA in isoform 3.
VSP_015942
Alternative sequence105 – 15450Missing in isoform 3.
VSP_015943
Alternative sequence144 – 15411VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTL A in isoform 2.
VSP_015944
Alternative sequence144 – 15411VSTVSQTMGER → TMEEYMNKPTF in isoform 4.
VSP_040384

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 178755E8EA4E9ABE

FASTA15418,280
        10         20         30         40         50         60 
MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV DWKELKLVYK 

        70         80         90        100        110        120 
RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE LDIIFNFEKA YFILDEFIIG 

       130        140        150 
GEIQETSKKI AVKAIEDSDM LQEVSTVSQT MGER 

« Hide

Isoform 2 [UniParc].

Checksum: 4588CDFD04DA9DBC
Show »

FASTA16419,445
Isoform 4 [UniParc].

Checksum: B0511E775FBED447
Show »

FASTA15418,477
Isoform 3 [UniParc].

Checksum: 16193FBF026884ED
Show »

FASTA10412,655

References

« Hide 'large scale' references
[1]"Adaptins: the final recount."
Boehm M., Bonifacino J.S.
Mol. Biol. Cell 12:2907-2920(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Brain and Lymph.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF393369 mRNA. Translation: AAL09586.1.
AK299026 mRNA. Translation: BAG61103.1.
AC012512 Genomic DNA. No translation available.
AC093884 Genomic DNA. No translation available.
BC009606 mRNA. Translation: AAH09606.1.
BC021898 mRNA. Translation: AAH21898.1.
RefSeqNP_001034658.1. NM_001039569.1.
UniGeneHs.632555.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HMYX-ray7.00S1-154[»]
ProteinModelPortalQ96PC3.
SMRQ96PC3. Positions 1-145.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126229. 2 interactions.
IntActQ96PC3. 4 interactions.
STRING9606.ENSP00000379891.

PTM databases

PhosphoSiteQ96PC3.

Polymorphism databases

DMDM21541959.

Proteomic databases

PaxDbQ96PC3.
PRIDEQ96PC3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000396654; ENSP00000379891; ENSG00000152056. [Q96PC3-4]
ENST00000415298; ENSP00000401705; ENSG00000152056. [Q96PC3-3]
ENST00000423110; ENSP00000406686; ENSG00000152056. [Q96PC3-3]
ENST00000443700; ENSP00000397155; ENSG00000152056. [Q96PC3-2]
ENST00000446015; ENSP00000388738; ENSG00000152056. [Q96PC3-1]
GeneID130340.
KEGGhsa:130340.
UCSCuc002vnn.3. human. [Q96PC3-4]
uc010fwx.2. human. [Q96PC3-1]

Organism-specific databases

CTD130340.
GeneCardsGC02M224616.
HGNCHGNC:18971. AP1S3.
neXtProtNX_Q96PC3.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5030.
HOGENOMHOG000185227.
HOVERGENHBG050517.
InParanoidQ96PC3.
KOK12395.
OMAEYMNKPA.
OrthoDBEOG7S7SGC.
PhylomeDBQ96PC3.
TreeFamTF312921.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ96PC3.
BgeeQ96PC3.
CleanExHS_AP1S3.
GenevestigatorQ96PC3.

Family and domain databases

InterProIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERPTHR11753. PTHR11753. 1 hit.
PfamPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMSSF64356. SSF64356. 1 hit.
PROSITEPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi130340.
NextBio82726.
PROQ96PC3.

Entry information

Entry nameAP1S3_HUMAN
AccessionPrimary (citable) accession number: Q96PC3
Secondary accession number(s): B4DQZ1, Q8WTY1, Q96DD1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM