Q96PC3 (AP1S3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit sigma-3

Alternative name(s):
Adapter-related protein complex 1 sigma-1C subunit
Adaptor protein complex AP-1 sigma-1C subunit
Clathrin assembly protein complex 1 sigma-1C small chain
Golgi adaptor HA1/AP1 adaptin sigma-1C subunit
Sigma 1C subunit of AP-1 clathrin
Sigma-adaptin 1C
Sigma1C-adaptin

Gene names

Name:

AP1S3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).
Subcellular location	Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › clathrin-coated pit. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the adaptor complexes small subunit family.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Coated pit Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome cellular membrane organization Traceable author statement. Source: Reactome endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome regulation of defense response to virus by virus Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Traceable author statement. Source: Reactome cytosol Traceable author statement. Source: Reactome lysosomal membrane Traceable author statement. Source: Reactome membrane coat Inferred from electronic annotation. Source: InterPro trans-Golgi network membrane Traceable author statement. Source: Reactome
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96PC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96PC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTLA

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 4 (identifier: Q96PC3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → TMEEYMNKPTF

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96PC3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     98-104: VCELDII → TWPFARA
     105-154: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	AP-1 complex subunit sigma-3		PRO_0000193801
Natural variations
	Alternative sequence	98 – 104	7	VCELDII → TWPFARA in isoform 3.		VSP_015942
	Alternative sequence	105 – 154	50	Missing in isoform 3.		VSP_015943
	Alternative sequence	144 – 154	11	VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTL A in isoform 2.		VSP_015944
	Alternative sequence	144 – 154	11	VSTVSQTMGER → TMEEYMNKPTF in isoform 4.		VSP_040384

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 178755E8EA4E9ABE	FASTA	154	18,280
	10 20 30 40 50 60 MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV DWKELKLVYK 70 80 90 100 110 120 RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE LDIIFNFEKA YFILDEFIIG 130 140 150 GEIQETSKKI AVKAIEDSDM LQEVSTVSQT MGER
	Isoform 2 [UniParc]. Checksum: 4588CDFD04DA9DBC	FASTA	164	19,445
	10 20 30 40 50 60 MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV DWKELKLVYK 70 80 90 100 110 120 RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE LDIIFNFEKA YFILDEFIIG 130 140 150 160 GEIQETSKKI AVKAIEDSDM LQENRLSPRG RDCSEPRSCH CTLA
	Isoform 4 [UniParc]. Checksum: B0511E775FBED447	FASTA	154	18,477
	10 20 30 40 50 60 MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV DWKELKLVYK 70 80 90 100 110 120 RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE LDIIFNFEKA YFILDEFIIG 130 140 150 GEIQETSKKI AVKAIEDSDM LQETMEEYMN KPTF
	Isoform 3 [UniParc]. Checksum: 16193FBF026884ED	FASTA	104	12,655
	10 20 30 40 50 60 MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV DWKELKLVYK 70 80 90 100 RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNTWP FARA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adaptins: the final recount." Boehm M., Bonifacino J.S. Mol. Biol. Cell 12:2907-2920(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain and Lymph.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF393369 mRNA. Translation: AAL09586.1.
AK299026 mRNA. Translation: BAG61103.1.
AC012512 Genomic DNA. No translation available.
AC093884 Genomic DNA. No translation available.
BC009606 mRNA. Translation: AAH09606.1.
BC021898 mRNA. Translation: AAH21898.1.

IPI

IPI00060866.
IPI00654799.
IPI00915398.
IPI00937280.

RefSeq

NP_001034658.1. NM_001039569.1.

UniGene

Hs.632555.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HMY	X-ray	7.00	S	1-154	[»]

ProteinModelPortal

Q96PC3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96PC3. 1 interaction.

STRING

9606.ENSP00000379891.

PTM databases

PhosphoSite

Q96PC3.

Polymorphism databases

DMDM

21541959.

Proteomic databases

PaxDb

Q96PC3.

PRIDE

Q96PC3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000396654; ENSP00000379891; ENSG00000152056.
ENST00000415298; ENSP00000401705; ENSG00000152056.
ENST00000423110; ENSP00000406686; ENSG00000152056.
ENST00000443700; ENSP00000397155; ENSG00000152056.
ENST00000446015; ENSP00000388738; ENSG00000152056.

GeneID

130340.

KEGG

hsa:130340.

UCSC

uc002vnn.3. human.
uc010fwx.2. human.

Organism-specific databases

CTD

130340.

GeneCards

GC02M224580.

HGNC

HGNC:18971. AP1S3.

neXtProt

NX_Q96PC3.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5030.

HOGENOM

HOG000185227.

HOVERGEN

HBG050517.

InParanoid

Q96PC3.

KO

K12395.

OMA

TSKKTAV.

PhylomeDB

Q96PC3.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

Q96PC3.

Bgee

Q96PC3.

CleanEx

HS_AP1S3.

Genevestigator

Q96PC3.

GermOnline

ENSG00000152056. Homo sapiens.

Family and domain databases

InterPro

IPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]

PANTHER

PTHR11753. PTHR11753. 1 hit.

Pfam

PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]

PIRSF

PIRSF015588. AP_complex_sigma. 1 hit.

SUPFAM

SSF64356. Longin_like. 1 hit.

PROSITE

PS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

130340.

NextBio

82726.

Entry information

Entry name

AP1S3_HUMAN

Accession

Primary (citable) accession number: Q96PC3
Secondary accession number(s): B4DQZ1, Q8WTY1, Q96DD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.