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Q96PC3

- AP1S3_HUMAN

UniProt

Q96PC3 - AP1S3_HUMAN

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Protein

AP-1 complex subunit sigma-3

Gene

AP1S3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

  1. protein transporter activity Source: InterPro

GO - Biological processi

  1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  2. intracellular protein transport Source: InterPro
  3. membrane organization Source: Reactome
  4. post-Golgi vesicle-mediated transport Source: Reactome
  5. regulation of defense response to virus by virus Source: Reactome
  6. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
REACT_121399. MHC class II antigen presentation.
REACT_19287. Lysosome Vesicle Biogenesis.
REACT_19400. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit sigma-3
Alternative name(s):
Adaptor protein complex AP-1 subunit sigma-1C
Adaptor-related protein complex 1 subunit sigma-1C
Clathrin assembly protein complex 1 sigma-1C small chain
Golgi adaptor HA1/AP1 adaptin sigma-1C subunit
Sigma 1C subunit of AP-1 clathrin
Sigma-adaptin 1C
Sigma1C-adaptin
Gene namesi
Name:AP1S3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:18971. AP1S3.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. coated pit Source: UniProtKB-KW
  2. cytoplasmic vesicle membrane Source: Reactome
  3. cytosol Source: Reactome
  4. Golgi membrane Source: Reactome
  5. lysosomal membrane Source: Reactome
  6. membrane coat Source: InterPro
  7. trans-Golgi network membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

Orphaneti163931. Acrodermatitis continua suppurativa of Hallopeau.
247353. Generalized pustular psoriasis.
163927. Pustulosis palmaris et plantaris.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154AP-1 complex subunit sigma-3PRO_0000193801Add
BLAST

Proteomic databases

MaxQBiQ96PC3.
PaxDbiQ96PC3.
PRIDEiQ96PC3.

PTM databases

PhosphoSiteiQ96PC3.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiQ96PC3.
CleanExiHS_AP1S3.
ExpressionAtlasiQ96PC3. baseline and differential.
GenevestigatoriQ96PC3.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Protein-protein interaction databases

BioGridi126229. 5 interactions.
IntActiQ96PC3. 4 interactions.
STRINGi9606.ENSP00000379891.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HMYX-ray7.00S1-154[»]
ProteinModelPortaliQ96PC3.
SMRiQ96PC3. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00530000062839.
HOGENOMiHOG000185227.
HOVERGENiHBG050517.
InParanoidiQ96PC3.
KOiK12395.
OMAiSDMLQEN.
OrthoDBiEOG7S7SGC.
PhylomeDBiQ96PC3.
TreeFamiTF312921.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96PC3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV
60 70 80 90 100
DWKELKLVYK RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE
110 120 130 140 150
LDIIFNFEKA YFILDEFIIG GEIQETSKKI AVKAIEDSDM LQEVSTVSQT

MGER
Length:154
Mass (Da):18,280
Last modified:December 1, 2001 - v1
Checksum:i178755E8EA4E9ABE
GO
Isoform 2 (identifier: Q96PC3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTLA

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:164
Mass (Da):19,445
Checksum:i4588CDFD04DA9DBC
GO
Isoform 4 (identifier: Q96PC3-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-154: VSTVSQTMGER → TMEEYMNKPTF

Note: No experimental confirmation available.

Show »
Length:154
Mass (Da):18,477
Checksum:iB0511E775FBED447
GO
Isoform 3 (identifier: Q96PC3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-104: VCELDII → TWPFARA
     105-154: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:104
Mass (Da):12,655
Checksum:i16193FBF026884ED
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 1047VCELDII → TWPFARA in isoform 3. 1 PublicationVSP_015942
Alternative sequencei105 – 15450Missing in isoform 3. 1 PublicationVSP_015943Add
BLAST
Alternative sequencei144 – 15411VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTL A in isoform 2. 1 PublicationVSP_015944Add
BLAST
Alternative sequencei144 – 15411VSTVSQTMGER → TMEEYMNKPTF in isoform 4. 1 PublicationVSP_040384Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393369 mRNA. Translation: AAL09586.1.
AK299026 mRNA. Translation: BAG61103.1.
AC012512 Genomic DNA. No translation available.
AC093884 Genomic DNA. No translation available.
BC009606 mRNA. Translation: AAH09606.1.
BC021898 mRNA. Translation: AAH21898.1.
CCDSiCCDS42827.1. [Q96PC3-4]
RefSeqiNP_001034658.1. NM_001039569.1. [Q96PC3-4]
UniGeneiHs.632555.

Genome annotation databases

EnsembliENST00000396654; ENSP00000379891; ENSG00000152056. [Q96PC3-4]
ENST00000415298; ENSP00000401705; ENSG00000152056. [Q96PC3-3]
ENST00000443700; ENSP00000397155; ENSG00000152056. [Q96PC3-2]
ENST00000446015; ENSP00000388738; ENSG00000152056. [Q96PC3-1]
GeneIDi130340.
KEGGihsa:130340.
UCSCiuc002vnn.3. human. [Q96PC3-4]
uc010fwx.2. human. [Q96PC3-1]

Polymorphism databases

DMDMi21541959.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393369 mRNA. Translation: AAL09586.1 .
AK299026 mRNA. Translation: BAG61103.1 .
AC012512 Genomic DNA. No translation available.
AC093884 Genomic DNA. No translation available.
BC009606 mRNA. Translation: AAH09606.1 .
BC021898 mRNA. Translation: AAH21898.1 .
CCDSi CCDS42827.1. [Q96PC3-4 ]
RefSeqi NP_001034658.1. NM_001039569.1. [Q96PC3-4 ]
UniGenei Hs.632555.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HMY X-ray 7.00 S 1-154 [» ]
ProteinModelPortali Q96PC3.
SMRi Q96PC3. Positions 1-145.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126229. 5 interactions.
IntActi Q96PC3. 4 interactions.
STRINGi 9606.ENSP00000379891.

PTM databases

PhosphoSitei Q96PC3.

Polymorphism databases

DMDMi 21541959.

Proteomic databases

MaxQBi Q96PC3.
PaxDbi Q96PC3.
PRIDEi Q96PC3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000396654 ; ENSP00000379891 ; ENSG00000152056 . [Q96PC3-4 ]
ENST00000415298 ; ENSP00000401705 ; ENSG00000152056 . [Q96PC3-3 ]
ENST00000443700 ; ENSP00000397155 ; ENSG00000152056 . [Q96PC3-2 ]
ENST00000446015 ; ENSP00000388738 ; ENSG00000152056 . [Q96PC3-1 ]
GeneIDi 130340.
KEGGi hsa:130340.
UCSCi uc002vnn.3. human. [Q96PC3-4 ]
uc010fwx.2. human. [Q96PC3-1 ]

Organism-specific databases

CTDi 130340.
GeneCardsi GC02M224616.
HGNCi HGNC:18971. AP1S3.
MIMi 615781. gene.
neXtProti NX_Q96PC3.
Orphaneti 163931. Acrodermatitis continua suppurativa of Hallopeau.
247353. Generalized pustular psoriasis.
163927. Pustulosis palmaris et plantaris.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5030.
GeneTreei ENSGT00530000062839.
HOGENOMi HOG000185227.
HOVERGENi HBG050517.
InParanoidi Q96PC3.
KOi K12395.
OMAi SDMLQEN.
OrthoDBi EOG7S7SGC.
PhylomeDBi Q96PC3.
TreeFami TF312921.

Enzyme and pathway databases

Reactomei REACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
REACT_121399. MHC class II antigen presentation.
REACT_19287. Lysosome Vesicle Biogenesis.
REACT_19400. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSi AP1S3. human.
GenomeRNAii 130340.
NextBioi 82726.
PROi Q96PC3.
SOURCEi Search...

Gene expression databases

Bgeei Q96PC3.
CleanExi HS_AP1S3.
ExpressionAtlasi Q96PC3. baseline and differential.
Genevestigatori Q96PC3.

Family and domain databases

InterProi IPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view ]
PANTHERi PTHR11753. PTHR11753. 1 hit.
Pfami PF01217. Clat_adaptor_s. 1 hit.
[Graphical view ]
PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMi SSF64356. SSF64356. 1 hit.
PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Brain and Lymph.

Entry informationi

Entry nameiAP1S3_HUMAN
AccessioniPrimary (citable) accession number: Q96PC3
Secondary accession number(s): B4DQZ1, Q8WTY1, Q96DD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3