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Q96PC3

- AP1S3_HUMAN

UniProt

Q96PC3 - AP1S3_HUMAN

Protein

AP-1 complex subunit sigma-3

Gene

AP1S3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

    GO - Molecular functioni

    1. protein transporter activity Source: InterPro

    GO - Biological processi

    1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
    2. intracellular protein transport Source: InterPro
    3. membrane organization Source: Reactome
    4. post-Golgi vesicle-mediated transport Source: Reactome
    5. regulation of defense response to virus by virus Source: Reactome
    6. viral process Source: Reactome

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit sigma-3
    Alternative name(s):
    Adaptor protein complex AP-1 subunit sigma-1C
    Adaptor-related protein complex 1 subunit sigma-1C
    Clathrin assembly protein complex 1 sigma-1C small chain
    Golgi adaptor HA1/AP1 adaptin sigma-1C subunit
    Sigma 1C subunit of AP-1 clathrin
    Sigma-adaptin 1C
    Sigma1C-adaptin
    Gene namesi
    Name:AP1S3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:18971. AP1S3.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. coated pit Source: UniProtKB-SubCell
    2. cytoplasmic vesicle membrane Source: Reactome
    3. cytosol Source: Reactome
    4. Golgi membrane Source: Reactome
    5. lysosomal membrane Source: Reactome
    6. membrane coat Source: InterPro
    7. trans-Golgi network membrane Source: Reactome

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154AP-1 complex subunit sigma-3PRO_0000193801Add
    BLAST

    Proteomic databases

    MaxQBiQ96PC3.
    PaxDbiQ96PC3.
    PRIDEiQ96PC3.

    PTM databases

    PhosphoSiteiQ96PC3.

    Expressioni

    Tissue specificityi

    Widely expressed.

    Gene expression databases

    ArrayExpressiQ96PC3.
    BgeeiQ96PC3.
    CleanExiHS_AP1S3.
    GenevestigatoriQ96PC3.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

    Protein-protein interaction databases

    BioGridi126229. 2 interactions.
    IntActiQ96PC3. 4 interactions.
    STRINGi9606.ENSP00000379891.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HMYX-ray7.00S1-154[»]
    ProteinModelPortaliQ96PC3.
    SMRiQ96PC3. Positions 1-145.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5030.
    HOGENOMiHOG000185227.
    HOVERGENiHBG050517.
    InParanoidiQ96PC3.
    KOiK12395.
    OMAiSDMLQEN.
    OrthoDBiEOG7S7SGC.
    PhylomeDBiQ96PC3.
    TreeFamiTF312921.

    Family and domain databases

    InterProiIPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view]
    PANTHERiPTHR11753. PTHR11753. 1 hit.
    PfamiPF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96PC3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV    50
    DWKELKLVYK RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE 100
    LDIIFNFEKA YFILDEFIIG GEIQETSKKI AVKAIEDSDM LQEVSTVSQT 150
    MGER 154
    Length:154
    Mass (Da):18,280
    Last modified:December 1, 2001 - v1
    Checksum:i178755E8EA4E9ABE
    GO
    Isoform 2 (identifier: Q96PC3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         144-154: VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTLA

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:164
    Mass (Da):19,445
    Checksum:i4588CDFD04DA9DBC
    GO
    Isoform 4 (identifier: Q96PC3-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         144-154: VSTVSQTMGER → TMEEYMNKPTF

    Note: No experimental confirmation available.

    Show »
    Length:154
    Mass (Da):18,477
    Checksum:iB0511E775FBED447
    GO
    Isoform 3 (identifier: Q96PC3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         98-104: VCELDII → TWPFARA
         105-154: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:104
    Mass (Da):12,655
    Checksum:i16193FBF026884ED
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei98 – 1047VCELDII → TWPFARA in isoform 3. 1 PublicationVSP_015942
    Alternative sequencei105 – 15450Missing in isoform 3. 1 PublicationVSP_015943Add
    BLAST
    Alternative sequencei144 – 15411VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTL A in isoform 2. 1 PublicationVSP_015944Add
    BLAST
    Alternative sequencei144 – 15411VSTVSQTMGER → TMEEYMNKPTF in isoform 4. 1 PublicationVSP_040384Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF393369 mRNA. Translation: AAL09586.1.
    AK299026 mRNA. Translation: BAG61103.1.
    AC012512 Genomic DNA. No translation available.
    AC093884 Genomic DNA. No translation available.
    BC009606 mRNA. Translation: AAH09606.1.
    BC021898 mRNA. Translation: AAH21898.1.
    CCDSiCCDS42827.1. [Q96PC3-4]
    RefSeqiNP_001034658.1. NM_001039569.1. [Q96PC3-4]
    UniGeneiHs.632555.

    Genome annotation databases

    EnsembliENST00000396654; ENSP00000379891; ENSG00000152056. [Q96PC3-4]
    ENST00000415298; ENSP00000401705; ENSG00000152056. [Q96PC3-3]
    ENST00000443700; ENSP00000397155; ENSG00000152056. [Q96PC3-2]
    ENST00000446015; ENSP00000388738; ENSG00000152056. [Q96PC3-1]
    GeneIDi130340.
    KEGGihsa:130340.
    UCSCiuc002vnn.3. human. [Q96PC3-4]
    uc010fwx.2. human. [Q96PC3-1]

    Polymorphism databases

    DMDMi21541959.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF393369 mRNA. Translation: AAL09586.1 .
    AK299026 mRNA. Translation: BAG61103.1 .
    AC012512 Genomic DNA. No translation available.
    AC093884 Genomic DNA. No translation available.
    BC009606 mRNA. Translation: AAH09606.1 .
    BC021898 mRNA. Translation: AAH21898.1 .
    CCDSi CCDS42827.1. [Q96PC3-4 ]
    RefSeqi NP_001034658.1. NM_001039569.1. [Q96PC3-4 ]
    UniGenei Hs.632555.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HMY X-ray 7.00 S 1-154 [» ]
    ProteinModelPortali Q96PC3.
    SMRi Q96PC3. Positions 1-145.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126229. 2 interactions.
    IntActi Q96PC3. 4 interactions.
    STRINGi 9606.ENSP00000379891.

    PTM databases

    PhosphoSitei Q96PC3.

    Polymorphism databases

    DMDMi 21541959.

    Proteomic databases

    MaxQBi Q96PC3.
    PaxDbi Q96PC3.
    PRIDEi Q96PC3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396654 ; ENSP00000379891 ; ENSG00000152056 . [Q96PC3-4 ]
    ENST00000415298 ; ENSP00000401705 ; ENSG00000152056 . [Q96PC3-3 ]
    ENST00000443700 ; ENSP00000397155 ; ENSG00000152056 . [Q96PC3-2 ]
    ENST00000446015 ; ENSP00000388738 ; ENSG00000152056 . [Q96PC3-1 ]
    GeneIDi 130340.
    KEGGi hsa:130340.
    UCSCi uc002vnn.3. human. [Q96PC3-4 ]
    uc010fwx.2. human. [Q96PC3-1 ]

    Organism-specific databases

    CTDi 130340.
    GeneCardsi GC02M224616.
    HGNCi HGNC:18971. AP1S3.
    MIMi 615781. gene.
    neXtProti NX_Q96PC3.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5030.
    HOGENOMi HOG000185227.
    HOVERGENi HBG050517.
    InParanoidi Q96PC3.
    KOi K12395.
    OMAi SDMLQEN.
    OrthoDBi EOG7S7SGC.
    PhylomeDBi Q96PC3.
    TreeFami TF312921.

    Enzyme and pathway databases

    Reactomei REACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.

    Miscellaneous databases

    GenomeRNAii 130340.
    NextBioi 82726.
    PROi Q96PC3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96PC3.
    Bgeei Q96PC3.
    CleanExi HS_AP1S3.
    Genevestigatori Q96PC3.

    Family and domain databases

    InterProi IPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view ]
    PANTHERi PTHR11753. PTHR11753. 1 hit.
    Pfami PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Tissue: Brain and Lymph.

    Entry informationi

    Entry nameiAP1S3_HUMAN
    AccessioniPrimary (citable) accession number: Q96PC3
    Secondary accession number(s): B4DQZ1, Q8WTY1, Q96DD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3