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Q96P71 (NECA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-terminal EF-hand calcium-binding protein 3
Alternative name(s):
Amyloid beta A4 protein-binding family A member 2-binding protein
Nek2-interacting protein 1
Neuronal calcium-binding protein 3
X11L-binding protein 51
Gene names
Name:NECAB3
Synonyms:APBA2BP, NIP1, SYTIP2, XB51
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid. Ref.7

Subunit structure

Interacts with the N-terminal domain of APBA2. Interacts with NEK2. Ref.2 Ref.7

Subcellular location

Golgi apparatus Ref.2 Ref.7.

Tissue specificity

Strongly expressed in heart and skeletal muscle, moderately in brain and pancreas. Ref.1 Ref.7

Post-translational modification

Phosphorylated by NEK2.

Sequence similarities

Contains 1 ABM domain.

Contains 1 EF-hand domain.

Sequence caution

The sequence BAB14649.1 differs from that shown. Reason: Frameshift at position 56.

The sequence BAB16413.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.
Isoform 2 (identifier: Q96P71-1)

Also known as: XB51-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q96P71-2)

Also known as: XB51-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     242-275: Missing.
Isoform 3 (identifier: Q96P71-3)

The sequence of this isoform differs from the canonical sequence as follows:
     176-203: SDAESVEAQSRLCGSRRAGRRALRSVSR → YVRVLSTCGASAQAPIVPPFQIPTVPAS
     204-396: Missing.
Note: May result from the retention of an intron in the cDNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396N-terminal EF-hand calcium-binding protein 3
PRO_0000073863

Regions

Domain36 – 7136EF-hand
Domain304 – 36663ABM
Calcium binding49 – 6012 Potential

Natural variations

Alternative sequence176 – 20328SDAES…RSVSR → YVRVLSTCGASAQAPIVPPF QIPTVPAS in isoform 3.
VSP_000737
Alternative sequence204 – 396193Missing in isoform 3.
VSP_000738
Alternative sequence242 – 27534Missing in isoform 1.
VSP_000739
Natural variant2541P → L.
Corresponds to variant rs17124890 [ dbSNP | Ensembl ].
VAR_048643

Experimental info

Sequence conflict3831T → I in AAG28415. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (XB51-alpha) [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: 9B24503AA629CB45

FASTA39644,350
        10         20         30         40         50         60 
MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE 

        70         80         90        100        110        120 
FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV 

       130        140        150        160        170        180 
LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRSDAES 

       190        200        210        220        230        240 
VEAQSRLCGS RRAGRRALRS VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC 

       250        260        270        280        290        300 
KVRAVGPGPH KGGPSWYPPE PGPCWRPGPH SVPSQAPRLE PLREEDLAKG PDLHILMAQR 

       310        320        330        340        350        360 
QVQVAEEGLQ DFHRALRCYV DFTGAQSHCL HVSAQKMLDG ASFTLYEFWQ DEASWRRHQQ 

       370        380        390 
SPGSKAFQRI LIDHLRAPDT LTTVFFPASW WIMNNN 

« Hide

Isoform 1 (XB51-beta) [UniParc].

Checksum: 946EFAB4F5D97AC4
Show »

FASTA36240,796
Isoform 3 [UniParc].

Checksum: 5F7361BCCC745238
Show »

FASTA20322,251

References

« Hide 'large scale' references
[1]"NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern."
Sugita S., Ho A., Suedhof T.C.
Neuroscience 112:51-63(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi."
Yoo J.C., Chang J.R., Kim S.H., Jang S.K., Wolgemuth D.J., Kim K., Rhee K.
Exp. Cell Res. 292:393-402(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH NEK2, SUBCELLULAR LOCATION.
Tissue: Liver.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Placenta and Skeletal muscle.
[4]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"Regulation of X11L-dependent amyloid precursor protein metabolism by XB51, a novel X11L-binding protein."
Lee D.-S., Tomita S., Kirino Y., Suzuki T.
J. Biol. Chem. 275:23134-23138(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 65-396 (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH APBA2.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF193759 mRNA. Translation: AAG28415.1.
AF409141 mRNA. Translation: AAL01118.1.
AK023706 mRNA. Translation: BAB14649.1. Frameshift.
AK291895 mRNA. Translation: BAF84584.1.
AL121906 Genomic DNA. Translation: CAI22127.1.
AL121906 Genomic DNA. Translation: CAI22128.1.
AL121906 Genomic DNA. Translation: CAI22129.1.
CH471077 Genomic DNA. Translation: EAW76302.1.
CH471077 Genomic DNA. Translation: EAW76304.1.
BC047673 mRNA. Translation: AAH47673.1.
AB039947 mRNA. Translation: BAB16413.1. Different initiation.
RefSeqNP_112508.3. NM_031231.3.
NP_112509.3. NM_031232.3.
UniGeneHs.516986.

3D structure databases

ProteinModelPortalQ96P71.
SMRQ96P71. Positions 38-102, 294-380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122006. 5 interactions.
IntActQ96P71. 1 interaction.

PTM databases

PhosphoSiteQ96P71.

Polymorphism databases

DMDM41688800.

Proteomic databases

PaxDbQ96P71.
PRIDEQ96P71.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246190; ENSP00000246190; ENSG00000125967. [Q96P71-1]
ENST00000375238; ENSP00000364386; ENSG00000125967. [Q96P71-2]
GeneID63941.
KEGGhsa:63941.
UCSCuc002wzm.4. human. [Q96P71-2]
uc002wzn.4. human. [Q96P71-1]

Organism-specific databases

CTD63941.
GeneCardsGC20M032244.
HGNCHGNC:15851. NECAB3.
MIM612478. gene.
neXtProtNX_Q96P71.
PharmGKBPA24871.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG70920.
HOVERGENHBG050433.
InParanoidQ96P71.
OMAHRALCCY.
OrthoDBEOG7BS49N.
PhylomeDBQ96P71.
TreeFamTF331029.

Gene expression databases

ArrayExpressQ96P71.
BgeeQ96P71.
CleanExHS_NECAB3.
GenevestigatorQ96P71.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR007138. Antibiotic_mOase.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SUPFAMSSF54909. SSF54909. 1 hit.
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNECAB3. human.
GeneWikiAPBA2BP.
GenomeRNAi63941.
NextBio65710.
PROQ96P71.
SOURCESearch...

Entry information

Entry nameNECA3_HUMAN
AccessionPrimary (citable) accession number: Q96P71
Secondary accession number(s): A8K780 expand/collapse secondary AC list , E1P5N2, Q5JWF5, Q5JWF6, Q5JWF7, Q86VV1, Q9H433, Q9H8G8, Q9HBW7, Q9HCQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM