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Q96P71

- NECA3_HUMAN

UniProt

Q96P71 - NECA3_HUMAN

Protein

N-terminal EF-hand calcium-binding protein 3

Gene

NECAB3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (28 Feb 2003)
      Previous versions | rss
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    Functioni

    Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi49 – 6012PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. protein metabolic process Source: UniProtKB
    2. protein secretion Source: UniProtKB
    3. regulation of amyloid precursor protein biosynthetic process Source: UniProtKB

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-terminal EF-hand calcium-binding protein 3
    Alternative name(s):
    Amyloid beta A4 protein-binding family A member 2-binding protein
    Nek2-interacting protein 1
    Neuronal calcium-binding protein 3
    X11L-binding protein 51
    Gene namesi
    Name:NECAB3
    Synonyms:APBA2BP, NIP1, SYTIP2, XB51
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:15851. NECAB3.

    Subcellular locationi

    Golgi apparatus 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB
    3. Golgi cis cisterna Source: UniProtKB
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24871.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396N-terminal EF-hand calcium-binding protein 3PRO_0000073863Add
    BLAST

    Post-translational modificationi

    Phosphorylated by NEK2.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ96P71.
    PRIDEiQ96P71.

    PTM databases

    PhosphoSiteiQ96P71.

    Expressioni

    Tissue specificityi

    Strongly expressed in heart and skeletal muscle, moderately in brain and pancreas.2 Publications

    Gene expression databases

    ArrayExpressiQ96P71.
    BgeeiQ96P71.
    CleanExiHS_NECAB3.
    GenevestigatoriQ96P71.

    Interactioni

    Subunit structurei

    Interacts with the N-terminal domain of APBA2. Interacts with NEK2.2 Publications

    Protein-protein interaction databases

    BioGridi122006. 5 interactions.
    IntActiQ96P71. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96P71.
    SMRiQ96P71. Positions 40-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 7136EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini296 – 38590ABMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ABM domain.Curated
    Contains 1 EF-hand domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG70920.
    HOVERGENiHBG050433.
    InParanoidiQ96P71.
    OMAiHRALCCY.
    OrthoDBiEOG7BS49N.
    PhylomeDBiQ96P71.
    TreeFamiTF331029.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF03992. ABM. 1 hit.
    PF13202. EF-hand_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF54909. SSF54909. 1 hit.
    PROSITEiPS51725. ABM. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

    Isoform 2 (identifier: Q96P71-1) [UniParc]FASTAAdd to Basket

    Also known as: XB51-alpha

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK    50
    NDDGKLSFEE FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS 100
    EHLGVYRPVL AALESLNRAV LAAMDATKLE YERASKVDQF VTRFLLRETV 150
    SQLQALQSSL EGASDTLEAQ AHGWRSDAES VEAQSRLCGS RRAGRRALRS 200
    VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC KVRAVGPGPH 250
    KGGPSWYPPE PGPCWRPGPH SVPSQAPRLE PLREEDLAKG PDLHILMAQR 300
    QVQVAEEGLQ DFHRALRCYV DFTGAQSHCL HVSAQKMLDG ASFTLYEFWQ 350
    DEASWRRHQQ SPGSKAFQRI LIDHLRAPDT LTTVFFPASW WIMNNN 396
    Length:396
    Mass (Da):44,350
    Last modified:February 28, 2003 - v2
    Checksum:i9B24503AA629CB45
    GO
    Isoform 1 (identifier: Q96P71-2) [UniParc]FASTAAdd to Basket

    Also known as: XB51-beta

    The sequence of this isoform differs from the canonical sequence as follows:
         242-275: Missing.

    Show »
    Length:362
    Mass (Da):40,796
    Checksum:i946EFAB4F5D97AC4
    GO
    Isoform 3 (identifier: Q96P71-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         176-203: SDAESVEAQSRLCGSRRAGRRALRSVSR → YVRVLSTCGASAQAPIVPPFQIPTVPAS
         204-396: Missing.

    Note: May result from the retention of an intron in the cDNA.

    Show »
    Length:203
    Mass (Da):22,251
    Checksum:i5F7361BCCC745238
    GO

    Sequence cautioni

    The sequence BAB14649.1 differs from that shown. Reason: Frameshift at position 56.
    The sequence BAB16413.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti383 – 3831T → I in AAG28415. (PubMed:12044471)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti254 – 2541P → L.
    Corresponds to variant rs17124890 [ dbSNP | Ensembl ].
    VAR_048643

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei176 – 20328SDAES…RSVSR → YVRVLSTCGASAQAPIVPPF QIPTVPAS in isoform 3. 1 PublicationVSP_000737Add
    BLAST
    Alternative sequencei204 – 396193Missing in isoform 3. 1 PublicationVSP_000738Add
    BLAST
    Alternative sequencei242 – 27534Missing in isoform 1. 4 PublicationsVSP_000739Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF193759 mRNA. Translation: AAG28415.1.
    AF409141 mRNA. Translation: AAL01118.1.
    AK023706 mRNA. Translation: BAB14649.1. Frameshift.
    AK291895 mRNA. Translation: BAF84584.1.
    AL121906 Genomic DNA. Translation: CAI22127.1.
    AL121906 Genomic DNA. Translation: CAI22128.1.
    AL121906 Genomic DNA. Translation: CAI22129.1.
    CH471077 Genomic DNA. Translation: EAW76302.1.
    CH471077 Genomic DNA. Translation: EAW76304.1.
    BC047673 mRNA. Translation: AAH47673.1.
    AB039947 mRNA. Translation: BAB16413.1. Different initiation.
    CCDSiCCDS42866.1. [Q96P71-1]
    CCDS42867.1. [Q96P71-2]
    RefSeqiNP_112508.3. NM_031231.3. [Q96P71-2]
    NP_112509.3. NM_031232.3. [Q96P71-1]
    UniGeneiHs.516986.

    Genome annotation databases

    EnsembliENST00000246190; ENSP00000246190; ENSG00000125967. [Q96P71-1]
    ENST00000375238; ENSP00000364386; ENSG00000125967. [Q96P71-2]
    GeneIDi63941.
    KEGGihsa:63941.
    UCSCiuc002wzm.4. human. [Q96P71-2]
    uc002wzn.4. human. [Q96P71-1]

    Polymorphism databases

    DMDMi41688800.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF193759 mRNA. Translation: AAG28415.1 .
    AF409141 mRNA. Translation: AAL01118.1 .
    AK023706 mRNA. Translation: BAB14649.1 . Frameshift.
    AK291895 mRNA. Translation: BAF84584.1 .
    AL121906 Genomic DNA. Translation: CAI22127.1 .
    AL121906 Genomic DNA. Translation: CAI22128.1 .
    AL121906 Genomic DNA. Translation: CAI22129.1 .
    CH471077 Genomic DNA. Translation: EAW76302.1 .
    CH471077 Genomic DNA. Translation: EAW76304.1 .
    BC047673 mRNA. Translation: AAH47673.1 .
    AB039947 mRNA. Translation: BAB16413.1 . Different initiation.
    CCDSi CCDS42866.1. [Q96P71-1 ]
    CCDS42867.1. [Q96P71-2 ]
    RefSeqi NP_112508.3. NM_031231.3. [Q96P71-2 ]
    NP_112509.3. NM_031232.3. [Q96P71-1 ]
    UniGenei Hs.516986.

    3D structure databases

    ProteinModelPortali Q96P71.
    SMRi Q96P71. Positions 40-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122006. 5 interactions.
    IntActi Q96P71. 1 interaction.

    PTM databases

    PhosphoSitei Q96P71.

    Polymorphism databases

    DMDMi 41688800.

    Proteomic databases

    PaxDbi Q96P71.
    PRIDEi Q96P71.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000246190 ; ENSP00000246190 ; ENSG00000125967 . [Q96P71-1 ]
    ENST00000375238 ; ENSP00000364386 ; ENSG00000125967 . [Q96P71-2 ]
    GeneIDi 63941.
    KEGGi hsa:63941.
    UCSCi uc002wzm.4. human. [Q96P71-2 ]
    uc002wzn.4. human. [Q96P71-1 ]

    Organism-specific databases

    CTDi 63941.
    GeneCardsi GC20M032244.
    HGNCi HGNC:15851. NECAB3.
    MIMi 612478. gene.
    neXtProti NX_Q96P71.
    PharmGKBi PA24871.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG70920.
    HOVERGENi HBG050433.
    InParanoidi Q96P71.
    OMAi HRALCCY.
    OrthoDBi EOG7BS49N.
    PhylomeDBi Q96P71.
    TreeFami TF331029.

    Miscellaneous databases

    ChiTaRSi NECAB3. human.
    GeneWikii APBA2BP.
    GenomeRNAii 63941.
    NextBioi 65710.
    PROi Q96P71.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96P71.
    Bgeei Q96P71.
    CleanExi HS_NECAB3.
    Genevestigatori Q96P71.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF03992. ABM. 1 hit.
    PF13202. EF-hand_5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    PROSITEi PS51725. ABM. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern."
      Sugita S., Ho A., Suedhof T.C.
      Neuroscience 112:51-63(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi."
      Yoo J.C., Chang J.R., Kim S.H., Jang S.K., Wolgemuth D.J., Kim K., Rhee K.
      Exp. Cell Res. 292:393-402(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH NEK2, SUBCELLULAR LOCATION.
      Tissue: Liver.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Placenta and Skeletal muscle.
    4. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Regulation of X11L-dependent amyloid precursor protein metabolism by XB51, a novel X11L-binding protein."
      Lee D.-S., Tomita S., Kirino Y., Suzuki T.
      J. Biol. Chem. 275:23134-23138(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 65-396 (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH APBA2.
      Tissue: Brain.

    Entry informationi

    Entry nameiNECA3_HUMAN
    AccessioniPrimary (citable) accession number: Q96P71
    Secondary accession number(s): A8K780
    , E1P5N2, Q5JWF5, Q5JWF6, Q5JWF7, Q86VV1, Q9H433, Q9H8G8, Q9HBW7, Q9HCQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: February 28, 2003
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3