Q96P71 (NECA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-terminal EF-hand calcium-binding protein 3

Alternative name(s):
Amyloid beta A4 protein-binding family A member 2-binding protein
Nek2-interacting protein 1
Neuronal calcium-binding protein 3
X11L-binding protein 51

Gene names

Name:	NECAB3
Synonyms:	APBA2BP, NIP1, SYTIP2, XB51

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid. Ref.7
Subunit structure	Interacts with the N-terminal domain of APBA2. Interacts with NEK2. Ref.2 Ref.7
Subcellular location	Golgi apparatus Ref.2 Ref.7.
Tissue specificity	Strongly expressed in heart and skeletal muscle, moderately in brain and pancreas. Ref.1 Ref.7
Post-translational modification	Phosphorylated by NEK2.
Sequence similarities	Contains 1 ABM domain. Contains 1 EF-hand domain.
Sequence caution	The sequence BAB14649.1 differs from that shown. Reason: Frameshift at position 56. The sequence BAB16413.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calcium Metal-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein metabolic process Inferred from direct assay PubMed 12780348. Source: UniProtKB protein secretion Non-traceable author statement PubMed 12780348. Source: UniProtKB regulation of amyloid precursor protein biosynthetic process Inferred from direct assay Ref.7. Source: UniProtKB
Cellular_component	Golgi cis cisterna Inferred from direct assay Ref.7. Source: UniProtKB endoplasmic reticulum membrane Inferred from direct assay Ref.7. Source: UniProtKB nucleus Non-traceable author statement Ref.7. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 2 (identifier: Q96P71-1)

Also known as: XB51-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q96P71-2)

Also known as: XB51-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     242-275: Missing.

Isoform 3 (identifier: Q96P71-3)

The sequence of this isoform differs from the canonical sequence as follows:
     176-203: SDAESVEAQSRLCGSRRAGRRALRSVSR → YVRVLSTCGASAQAPIVPPFQIPTVPAS
     204-396: Missing.

Note: May result from the retention of an intron in the cDNA.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	N-terminal EF-hand calcium-binding protein 3		PRO_0000073863
Regions
	Domain	36 – 71	36	EF-hand
	Domain	304 – 366	63	ABM
	Calcium binding	49 – 60	12	Potential
Natural variations
	Alternative sequence	176 – 203	28	SDAES…RSVSR → YVRVLSTCGASAQAPIVPPF QIPTVPAS in isoform 3.		VSP_000737
	Alternative sequence	204 – 396	193	Missing in isoform 3.		VSP_000738
	Alternative sequence	242 – 275	34	Missing in isoform 1.		VSP_000739
	Natural variant	254	1	P → L. Corresponds to variant rs17124890 [ dbSNP | Ensembl ].		VAR_048643
Experimental info
	Sequence conflict	383	1	T → I in AAG28415. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (XB51-alpha) [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 9B24503AA629CB45	FASTA	396	44,350
	10 20 30 40 50 60 MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE 70 80 90 100 110 120 FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV 130 140 150 160 170 180 LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRSDAES 190 200 210 220 230 240 VEAQSRLCGS RRAGRRALRS VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC 250 260 270 280 290 300 KVRAVGPGPH KGGPSWYPPE PGPCWRPGPH SVPSQAPRLE PLREEDLAKG PDLHILMAQR 310 320 330 340 350 360 QVQVAEEGLQ DFHRALRCYV DFTGAQSHCL HVSAQKMLDG ASFTLYEFWQ DEASWRRHQQ 370 380 390 SPGSKAFQRI LIDHLRAPDT LTTVFFPASW WIMNNN
	Isoform 1 (XB51-beta) [UniParc]. Checksum: 946EFAB4F5D97AC4	FASTA	362	40,796
	10 20 30 40 50 60 MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE 70 80 90 100 110 120 FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV 130 140 150 160 170 180 LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRSDAES 190 200 210 220 230 240 VEAQSRLCGS RRAGRRALRS VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC 250 260 270 280 290 300 KAPRLEPLRE EDLAKGPDLH ILMAQRQVQV AEEGLQDFHR ALRCYVDFTG AQSHCLHVSA 310 320 330 340 350 360 QKMLDGASFT LYEFWQDEAS WRRHQQSPGS KAFQRILIDH LRAPDTLTTV FFPASWWIMN NN
	Isoform 3 [UniParc]. Checksum: 5F7361BCCC745238	FASTA	203	22,251
	10 20 30 40 50 60 MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE 70 80 90 100 110 120 FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV 130 140 150 160 170 180 LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRYVRVL 190 200 STCGASAQAP IVPPFQIPTV PAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern." Sugita S., Ho A., Suedhof T.C. Neuroscience 112:51-63(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi." Yoo J.C., Chang J.R., Kim S.H., Jang S.K., Wolgemuth D.J., Kim K., Rhee K. Exp. Cell Res. 292:393-402(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH NEK2, SUBCELLULAR LOCATION. Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Placenta and Skeletal muscle.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"Regulation of X11L-dependent amyloid precursor protein metabolism by XB51, a novel X11L-binding protein." Lee D.-S., Tomita S., Kirino Y., Suzuki T. J. Biol. Chem. 275:23134-23138(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 65-396 (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH APBA2. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF193759 mRNA. Translation: AAG28415.1. AF409141 mRNA. Translation: AAL01118.1. AK023706 mRNA. Translation: BAB14649.1. Frameshift. AK291895 mRNA. Translation: BAF84584.1. AL121906 Genomic DNA. Translation: CAI22127.1. AL121906 Genomic DNA. Translation: CAI22128.1. AL121906 Genomic DNA. Translation: CAI22129.1. CH471077 Genomic DNA. Translation: EAW76302.1. CH471077 Genomic DNA. Translation: EAW76304.1. BC047673 mRNA. Translation: AAH47673.1. AB039947 mRNA. Translation: BAB16413.1. Different initiation.
IPI	IPI00102440. IPI00218453. IPI00295304.
RefSeq	NP_112508.3. NM_031231.3. NP_112509.3. NM_031232.3.
UniGene	Hs.516986.
3D structure databases
ProteinModelPortal	Q96P71.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96P71. 1 interaction.
PTM databases
PhosphoSite	Q96P71.
Polymorphism databases
DMDM	41688800.
Proteomic databases
PaxDb	Q96P71.
PRIDE	Q96P71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000246190; ENSP00000246190; ENSG00000125967. ENST00000375238; ENSP00000364386; ENSG00000125967. ENST00000439478; ENSP00000392064; ENSG00000125967.
GeneID	63941.
KEGG	hsa:63941.
UCSC	uc002wzm.4. human. uc002wzn.4. human.
Organism-specific databases
CTD	63941.
GeneCards	GC20M032244.
HGNC	HGNC:15851. NECAB3.
MIM	612478. gene.
neXtProt	NX_Q96P71.
PharmGKB	PA24871.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG70920.
HOVERGEN	HBG050433.
InParanoid	Q96P71.
OMA	LTAMDTT.
PhylomeDB	Q96P71.
Gene expression databases
ArrayExpress	Q96P71.
Bgee	Q96P71.
CleanEx	HS_NECAB3.
Genevestigator	Q96P71.
GermOnline	ENSG00000125967. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF03992. ABM. 1 hit. PF13202. EF_hand_3. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NECAB3. human.
GenomeRNAi	63941.
NextBio	65710.
SOURCE	Search...

Entry information

Entry name

NECA3_HUMAN

Accession

Primary (citable) accession number: Q96P71
Secondary accession number(s): A8K780 , E1P5N2, Q5JWF5, Q5JWF6, Q5JWF7, Q86VV1, Q9H433, Q9H8G8, Q9HBW7, Q9HCQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	February 28, 2003
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.