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Protein

WD repeat and FYVE domain-containing protein 2

Gene

WDFY2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (PubMed:17313651). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates (By similarity). Participates in transferrin receptor endocytosis (PubMed:16873553).By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri281 – 35272FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of fat cell differentiation Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ96P53.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and FYVE domain-containing protein 2
Alternative name(s):
Propeller-FYVE protein1 Publication
Short name:
Prof
WD40- and FYVE domain-containing protein 2
Zinc finger FYVE domain-containing protein 22
Gene namesi
Name:WDFY2
Synonyms:WDF2, ZFYVE22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20482. WDFY2.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134870231.

Polymorphism and mutation databases

BioMutaiWDFY2.
DMDMi51316902.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400WD repeat and FYVE domain-containing protein 2PRO_0000051336Add
BLAST

Proteomic databases

EPDiQ96P53.
MaxQBiQ96P53.
PaxDbiQ96P53.
PRIDEiQ96P53.

PTM databases

iPTMnetiQ96P53.
PhosphoSiteiQ96P53.

Expressioni

Gene expression databases

BgeeiQ96P53.
CleanExiHS_WDFY2.
ExpressionAtlasiQ96P53. baseline and differential.
GenevisibleiQ96P53. HS.

Interactioni

Subunit structurei

Homodimer (PubMed:16792529). Interacts (via WD repeats 1-3) with AKT1, AKT2, PRKCZ and PRKCI (PubMed:16792529). Interacts with VAMP2 (PubMed:17313651). Forms a complex with VAMP2 and PRKCZ (PubMed:17313651). Interacts with FOXO1 (By similarity). Forms a complex with AKT1 and FOXO1 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi125458. 25 interactions.
IntActiQ96P53. 1 interaction.
STRINGi9606.ENSP00000298125.

Structurei

3D structure databases

ProteinModelPortaliQ96P53.
SMRiQ96P53. Positions 12-278, 309-357, 369-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati22 – 6140WD 1Add
BLAST
Repeati66 – 10540WD 2Add
BLAST
Repeati112 – 15039WD 3Add
BLAST
Repeati153 – 19240WD 4Add
BLAST
Repeati197 – 23640WD 5Add
BLAST
Repeati240 – 27940WD 6Add
BLAST
Repeati364 – 39936WD 7Add
BLAST

Domaini

The FYVE-type zinc finger is essential for its vesicular localization.1 Publication

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri281 – 35272FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1409. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000007835.
HOVERGENiHBG054128.
InParanoidiQ96P53.
OMAiFVLEMEW.
OrthoDBiEOG7W153N.
PhylomeDBiQ96P53.
TreeFamiTF314470.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
3.30.40.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96P53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEIQPKPL TRKPILLQRM EGSQEVVNMA VIVPKEEGVI SVSEDRTVRV
60 70 80 90 100
WLKRDSGQYW PSVYHAMPSP CSCMSFNPET RRLSIGLDNG TISEFILSED
110 120 130 140 150
YNKMTPVKNY QAHQSRVTMI LFVLELEWVL STGQDKQFAW HCSESGQRLG
160 170 180 190 200
GYRTSAVASG LQFDVETRHV FIGDHSGQVT ILKLEQENCT LVTTFRGHTG
210 220 230 240 250
GVTALCWDPV QRVLFSGSSD HSVIMWDIGG RKGTAIELQG HNDRVQALSY
260 270 280 290 300
AQHTRQLISC GGDGGIVVWN MDVERQETPE WLDSDSCQKC DQPFFWNFKQ
310 320 330 340 350
MWDSKKIGLR QHHCRKCGKA VCGKCSSKRS SIPLMGFEFE VRVCDSCHEA
360 370 380 390 400
ITDEERAPTA TFHDSKHNIV HVHFDATRGW LLTSGTDKVI KLWDMTPVVS
Length:400
Mass (Da):45,154
Last modified:August 16, 2004 - v2
Checksum:i57868799A6C3C7C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101Y → C in AAL04162 (Ref. 1) Curated
Sequence conflicti197 – 1971G → E in AAL04162 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411978 mRNA. Translation: AAL04162.1.
AL136525, AL139183 Genomic DNA. Translation: CAH70585.1.
AL139183, AL136525 Genomic DNA. Translation: CAH73431.1.
CH471075 Genomic DNA. Translation: EAX08883.1.
BC014004 mRNA. Translation: AAH14004.1.
CCDSiCCDS9429.1.
RefSeqiNP_443182.1. NM_052950.3.
UniGeneiHs.208550.
Hs.46506.

Genome annotation databases

EnsembliENST00000298125; ENSP00000298125; ENSG00000139668.
GeneIDi115825.
KEGGihsa:115825.
UCSCiuc001vfp.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411978 mRNA. Translation: AAL04162.1.
AL136525, AL139183 Genomic DNA. Translation: CAH70585.1.
AL139183, AL136525 Genomic DNA. Translation: CAH73431.1.
CH471075 Genomic DNA. Translation: EAX08883.1.
BC014004 mRNA. Translation: AAH14004.1.
CCDSiCCDS9429.1.
RefSeqiNP_443182.1. NM_052950.3.
UniGeneiHs.208550.
Hs.46506.

3D structure databases

ProteinModelPortaliQ96P53.
SMRiQ96P53. Positions 12-278, 309-357, 369-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125458. 25 interactions.
IntActiQ96P53. 1 interaction.
STRINGi9606.ENSP00000298125.

PTM databases

iPTMnetiQ96P53.
PhosphoSiteiQ96P53.

Polymorphism and mutation databases

BioMutaiWDFY2.
DMDMi51316902.

Proteomic databases

EPDiQ96P53.
MaxQBiQ96P53.
PaxDbiQ96P53.
PRIDEiQ96P53.

Protocols and materials databases

DNASUi115825.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298125; ENSP00000298125; ENSG00000139668.
GeneIDi115825.
KEGGihsa:115825.
UCSCiuc001vfp.4. human.

Organism-specific databases

CTDi115825.
GeneCardsiWDFY2.
HGNCiHGNC:20482. WDFY2.
MIMi610418. gene.
neXtProtiNX_Q96P53.
PharmGKBiPA134870231.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1409. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000007835.
HOVERGENiHBG054128.
InParanoidiQ96P53.
OMAiFVLEMEW.
OrthoDBiEOG7W153N.
PhylomeDBiQ96P53.
TreeFamiTF314470.

Enzyme and pathway databases

SignaLinkiQ96P53.

Miscellaneous databases

GenomeRNAii115825.
PROiQ96P53.
SOURCEiSearch...

Gene expression databases

BgeeiQ96P53.
CleanExiHS_WDFY2.
ExpressionAtlasiQ96P53. baseline and differential.
GenevisibleiQ96P53. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
3.30.40.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "WD40- and FYVE-domain containing protein 2 (WDF2)."
    Hong W.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. Cited for: SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH AKT1; AKT2; PRKCZ AND PRKCI, DOMAIN FYVE-TYPE.
  6. "The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis."
    Hayakawa A., Leonard D., Murphy S., Hayes S., Soto M., Fogarty K., Standley C., Bellve K., Lambright D., Mello C., Corvera S.
    Proc. Natl. Acad. Sci. U.S.A. 103:11928-11933(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta."
    Fritzius T., Frey A.D., Schweneker M., Mayer D., Moelling K.
    FEBS J. 274:1552-1566(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VAMP2, COMPLEX FORMATION WITH VAMP2 AND PRKCZ, SUBCELLULAR LOCATION.
  8. "Akt- and Foxo1-interacting WD-repeat-FYVE protein promotes adipogenesis."
    Fritzius T., Moelling K.
    EMBO J. 27:1399-1410(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWDFY2_HUMAN
AccessioniPrimary (citable) accession number: Q96P53
Secondary accession number(s): B1AL86, Q96CS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.