Q96P48 (ARAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1

Alternative name(s):
Centaurin-delta-2
Short name=Cnt-d2

Gene names

Name:	ARAP1
Synonyms:	CENTD2, KIAA0782

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 By similarity. Ref.1
Subunit structure	Interacts with TNFRSF10A. Ref.11
Subcellular location	Cytoplasm. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Cell membrane. Note: Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading. Ref.1 Ref.11
Tissue specificity	Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea. Ref.1
Sequence similarities	Contains 1 Arf-GAP domain. Contains 4 PH domains. Contains 1 Ras-associating domain. Contains 1 Rho-GAP domain. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence BAA34502.2 differs from that shown. Reason: Erroneous initiation. The sequence BAD92710.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament reorganization involved in cell cycle Traceable author statement Ref.1. Source: UniProtKB negative regulation of stress fiber assembly Inferred from mutant phenotype Ref.1. Source: UniProtKB positive regulation of Cdc42 GTPase activity Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of filopodium assembly Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro regulation of cell shape Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of cellular component movement Inferred from mutant phenotype Ref.1. Source: UniProtKB small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	Golgi apparatus Inferred from direct assay Ref.1. Source: UniProtKB Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ARF GTPase activator activity Inferred from direct assay Ref.1. Source: UniProtKB Rho GTPase activator activity Inferred from direct assay Ref.1. Source: UniProtKB phosphatidylinositol-3,4,5-trisphosphate binding Inferred from direct assay PubMed 11804589. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TNFRSF10A	O00220	4	EBI-710003,EBI-518861
XRCC6	P12956	2	EBI-710003,EBI-353208

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 6 (identifier: Q96P48-6)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q96P48-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.
     241-249: APARVMTKK → MTLSGSRGQ

Isoform 2 (identifier: Q96P48-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.
     241-249: APARVMTKK → MTLSGSRGQ
     1320-1330: Missing.

Isoform 3 (identifier: Q96P48-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1320-1330: Missing.

Isoform 4 (identifier: Q96P48-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q96P48-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-760: Missing.
     761-767: QDRRARE → MDASGKG
     1320-1330: Missing.

Note: No experimental confirmation available.

Isoform 7 (identifier: Q96P48-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.
     604-664: Missing.
     1320-1330: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1450	1450	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1		PRO_0000074214
Regions
	Domain	6 – 70	65	SAM
	Domain	327 – 419	93	PH 1
	Domain	440 – 529	90	PH 2
	Domain	535 – 660	126	Arf-GAP
	Domain	743 – 850	108	PH 3
	Domain	954 – 1139	186	Rho-GAP
	Domain	1172 – 1261	90	Ras-associating
	Domain	1274 – 1396	123	PH 4
	Zinc finger	550 – 576	27	C4-type
Amino acid modifications
	Modified residue	229	1	Phosphoserine Ref.13
	Modified residue	354	1	Phosphothreonine Ref.10
	Modified residue	428	1	Phosphoserine Ref.15
	Modified residue	431	1	Phosphotyrosine Ref.13
	Modified residue	738	1	Phosphoserine Ref.14
	Modified residue	1435	1	Phosphoserine Ref.13 Ref.14
Natural variations
	Alternative sequence	1 – 760	760	Missing in isoform 5.		VSP_014998
	Alternative sequence	1 – 245	245	Missing in isoform 4 and isoform 7.		VSP_015000
	Alternative sequence	1 – 240	240	Missing in isoform 1 and isoform 2.		VSP_036607
	Alternative sequence	241 – 249	9	APARVMTKK → MTLSGSRGQ in isoform 1 and isoform 2.		VSP_036608
	Alternative sequence	604 – 664	61	Missing in isoform 7.		VSP_043530
	Alternative sequence	761 – 767	7	QDRRARE → MDASGKG in isoform 5.		VSP_015001
	Alternative sequence	1320 – 1330	11	Missing in isoform 2, isoform 3, isoform 5 and isoform 7.		VSP_000311
	Natural variant	358	1	R → Q. Corresponds to variant rs34976830 [ dbSNP | Ensembl ].		VAR_055529
	Natural variant	1047	1	Q → E. Ref.6 Corresponds to variant rs56200889 [ dbSNP | Ensembl ].		VAR_061023
Experimental info
	Sequence conflict	1408	1	V → M in AAH56401. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 6 [UniParc]. Last modified March 24, 2009. Version 3. Checksum: A4B6CCC28EC4CD0D	FASTA	1,450	162,192
	10 20 30 40 50 60 MAEAGDAALS VAEWLRALHL EQYTGLFEQH GLVWATECQG LSDTRLMDMG MLLPGHRRRI 70 80 90 100 110 120 LAGLLRAHTS PAPAPRPTPR PVPMKRHIFR SPPVPATPPE PLPTTTEDEG LPAAPPIPPR 130 140 150 160 170 180 RSCLPPTCFT TPSTAAPDPV LPPLPAKRHL AELSVPPVPP RTGPPRLLVS LPTKEEESLL 190 200 210 220 230 240 PSLSSPPQPQ SEEPLSTLPQ GPPQPPSPPP CPPEIPPKPV RLFPEFDDSD YDEVPEEGPG 250 260 270 280 290 300 APARVMTKKE EPPPSRVPRA VRVASLLSEG EELSGDDQGD EEEDDHAYEG VPNGGWHTSS 310 320 330 340 350 360 LSLSLPSTIA APHPMDGPPG GSTPVTPVIK AGWLDKNPPQ GSYIYQKRWV RLDTDHLRYF 370 380 390 400 410 420 DSNKDAYSKR FISVACISHV AAIGDQKFEV ITNNRTFAFR AESDVERKEW MQALQQAMAE 430 440 450 460 470 480 QRARARLSSA YLLGVPGSEQ PDRAGSLELR GFKNKLYVAV VGDKVQLYKN LEEYHLGIGI 490 500 510 520 530 540 TFIDMSVGNV KEVDRRSFDL TTPYRIFSFS ADSELEKEQW LEAMQGAIAE ALSTSEVAER 550 560 570 580 590 600 IWAAAPNRFC ADCGAPQPDW ASINLCVVIC KRCAGEHRGL GAGVSKVRSL KMDRKVWTET 610 620 630 640 650 660 LIELFLQLGN GAGNRFWAAN VPPSEALQPS SSPSTRRCHL EAKYREGKYR RYHPLFGNQE 670 680 690 700 710 720 ELDKALCAAV TTTDLAETQA LLGCGAGINC FSGDPEAPTP LALAEQAGQT LQMEFLRNNR 730 740 750 760 770 780 TTEVPRLDSM KPLEKHYSVV LPTVSHSGFL YKTASAGKLL QDRRAREEFS RRWCVLGDGV 790 800 810 820 830 840 LSYFENERAV TPNGEIRASE IVCLAVPPPD THGFEHTFEV YTEGERLYLF GLESAEQAHE 850 860 870 880 890 900 WVKCIAKAFV PPLAEDLLAR DFERLGRLPY KAGLSLQRAQ EGWFSLSGSE LRAVFPEGPC 910 920 930 940 950 960 EEPLQLRKLQ ELSIQGDSEN QVLVLVERRR TLYIQGERRL DFMGWLGAIQ KAAASMGDTL 970 980 990 1000 1010 1020 SEQQLGDSDI PVIVYRCVDY ITQCGLTSEG IYRKCGQTSK TQRLLESLRQ DARSVHLKEG 1030 1040 1050 1060 1070 1080 EQHVDDVSSA LKRFLRDLPD GLFTRAQRLT WLEASEIEDE EEKVSRYREL LVRLPPVNRA 1090 1100 1110 1120 1130 1140 TVKALISHLY CVQCFSDTNQ MNVHNLAIVF GPTLFQTDGQ DYKAGRVVED LINHYVVVFS 1150 1160 1170 1180 1190 1200 VDEEELRKQR EEITAIVKMR VAGTASGTQH AGDFICTVYL EEKKAETEQH IKVPASMTAE 1210 1220 1230 1240 1250 1260 ELTLEILDRR NVGIREKDYW TCFEVNEREE AERPLHFAEK VLPILHGLGT DSHLVVKKHQ 1270 1280 1290 1300 1310 1320 AMEAMLLYLA SRVGDTKHGM MKFREDRSLL GLGLPSGGFH DRYFILNSSC LRLYKEVRSQ 1330 1340 1350 1360 1370 1380 RPWSGAPETS HRPEKEWPIK SLKVYLGVKK KLRPPTCWGF TVVHETEKHE KQQWYLCCDT 1390 1400 1410 1420 1430 1440 QMELREWFAT FLFVQHDGLV WPSEPSRVSR AVPEVRLGSV SLIPLRGSEN EMRRSVAAFT 1450 ADPLSLLRNV
	Isoform 1 [UniParc]. Checksum: 31486141B6648495	FASTA	1,210	136,264
	10 20 30 40 50 60 MTLSGSRGQE EPPPSRVPRA VRVASLLSEG EELSGDDQGD EEEDDHAYEG VPNGGWHTSS 70 80 90 100 110 120 LSLSLPSTIA APHPMDGPPG GSTPVTPVIK AGWLDKNPPQ GSYIYQKRWV RLDTDHLRYF 130 140 150 160 170 180 DSNKDAYSKR FISVACISHV AAIGDQKFEV ITNNRTFAFR AESDVERKEW MQALQQAMAE 190 200 210 220 230 240 QRARARLSSA YLLGVPGSEQ PDRAGSLELR GFKNKLYVAV VGDKVQLYKN LEEYHLGIGI 250 260 270 280 290 300 TFIDMSVGNV KEVDRRSFDL TTPYRIFSFS ADSELEKEQW LEAMQGAIAE ALSTSEVAER 310 320 330 340 350 360 IWAAAPNRFC ADCGAPQPDW ASINLCVVIC KRCAGEHRGL GAGVSKVRSL KMDRKVWTET 370 380 390 400 410 420 LIELFLQLGN GAGNRFWAAN VPPSEALQPS SSPSTRRCHL EAKYREGKYR RYHPLFGNQE 430 440 450 460 470 480 ELDKALCAAV TTTDLAETQA LLGCGAGINC FSGDPEAPTP LALAEQAGQT LQMEFLRNNR 490 500 510 520 530 540 TTEVPRLDSM KPLEKHYSVV LPTVSHSGFL YKTASAGKLL QDRRAREEFS RRWCVLGDGV 550 560 570 580 590 600 LSYFENERAV TPNGEIRASE IVCLAVPPPD THGFEHTFEV YTEGERLYLF GLESAEQAHE 610 620 630 640 650 660 WVKCIAKAFV PPLAEDLLAR DFERLGRLPY KAGLSLQRAQ EGWFSLSGSE LRAVFPEGPC 670 680 690 700 710 720 EEPLQLRKLQ ELSIQGDSEN QVLVLVERRR TLYIQGERRL DFMGWLGAIQ KAAASMGDTL 730 740 750 760 770 780 SEQQLGDSDI PVIVYRCVDY ITQCGLTSEG IYRKCGQTSK TQRLLESLRQ DARSVHLKEG 790 800 810 820 830 840 EQHVDDVSSA LKRFLRDLPD GLFTRAQRLT WLEASEIEDE EEKVSRYREL LVRLPPVNRA 850 860 870 880 890 900 TVKALISHLY CVQCFSDTNQ MNVHNLAIVF GPTLFQTDGQ DYKAGRVVED LINHYVVVFS 910 920 930 940 950 960 VDEEELRKQR EEITAIVKMR VAGTASGTQH AGDFICTVYL EEKKAETEQH IKVPASMTAE 970 980 990 1000 1010 1020 ELTLEILDRR NVGIREKDYW TCFEVNEREE AERPLHFAEK VLPILHGLGT DSHLVVKKHQ 1030 1040 1050 1060 1070 1080 AMEAMLLYLA SRVGDTKHGM MKFREDRSLL GLGLPSGGFH DRYFILNSSC LRLYKEVRSQ 1090 1100 1110 1120 1130 1140 RPWSGAPETS HRPEKEWPIK SLKVYLGVKK KLRPPTCWGF TVVHETEKHE KQQWYLCCDT 1150 1160 1170 1180 1190 1200 QMELREWFAT FLFVQHDGLV WPSEPSRVSR AVPEVRLGSV SLIPLRGSEN EMRRSVAAFT 1210 ADPLSLLRNV
	Isoform 2 [UniParc]. Checksum: 95873FE63A8C3320	FASTA	1,199	135,066
	10 20 30 40 50 60 MTLSGSRGQE EPPPSRVPRA VRVASLLSEG EELSGDDQGD EEEDDHAYEG VPNGGWHTSS 70 80 90 100 110 120 LSLSLPSTIA APHPMDGPPG GSTPVTPVIK AGWLDKNPPQ GSYIYQKRWV RLDTDHLRYF 130 140 150 160 170 180 DSNKDAYSKR FISVACISHV AAIGDQKFEV ITNNRTFAFR AESDVERKEW MQALQQAMAE 190 200 210 220 230 240 QRARARLSSA YLLGVPGSEQ PDRAGSLELR GFKNKLYVAV VGDKVQLYKN LEEYHLGIGI 250 260 270 280 290 300 TFIDMSVGNV KEVDRRSFDL TTPYRIFSFS ADSELEKEQW LEAMQGAIAE ALSTSEVAER 310 320 330 340 350 360 IWAAAPNRFC ADCGAPQPDW ASINLCVVIC KRCAGEHRGL GAGVSKVRSL KMDRKVWTET 370 380 390 400 410 420 LIELFLQLGN GAGNRFWAAN VPPSEALQPS SSPSTRRCHL EAKYREGKYR RYHPLFGNQE 430 440 450 460 470 480 ELDKALCAAV TTTDLAETQA LLGCGAGINC FSGDPEAPTP LALAEQAGQT LQMEFLRNNR 490 500 510 520 530 540 TTEVPRLDSM KPLEKHYSVV LPTVSHSGFL YKTASAGKLL QDRRAREEFS RRWCVLGDGV 550 560 570 580 590 600 LSYFENERAV TPNGEIRASE IVCLAVPPPD THGFEHTFEV YTEGERLYLF GLESAEQAHE 610 620 630 640 650 660 WVKCIAKAFV PPLAEDLLAR DFERLGRLPY KAGLSLQRAQ EGWFSLSGSE LRAVFPEGPC 670 680 690 700 710 720 EEPLQLRKLQ ELSIQGDSEN QVLVLVERRR TLYIQGERRL DFMGWLGAIQ KAAASMGDTL 730 740 750 760 770 780 SEQQLGDSDI PVIVYRCVDY ITQCGLTSEG IYRKCGQTSK TQRLLESLRQ DARSVHLKEG 790 800 810 820 830 840 EQHVDDVSSA LKRFLRDLPD GLFTRAQRLT WLEASEIEDE EEKVSRYREL LVRLPPVNRA 850 860 870 880 890 900 TVKALISHLY CVQCFSDTNQ MNVHNLAIVF GPTLFQTDGQ DYKAGRVVED LINHYVVVFS 910 920 930 940 950 960 VDEEELRKQR EEITAIVKMR VAGTASGTQH AGDFICTVYL EEKKAETEQH IKVPASMTAE 970 980 990 1000 1010 1020 ELTLEILDRR NVGIREKDYW TCFEVNEREE AERPLHFAEK VLPILHGLGT DSHLVVKKHQ 1030 1040 1050 1060 1070 1080 AMEAMLLYLA SRVGDTKHGM MKFREDRSLL GLGLPSGGFH DRYFILNSSC LRLYKEVRSH 1090 1100 1110 1120 1130 1140 RPEKEWPIKS LKVYLGVKKK LRPPTCWGFT VVHETEKHEK QQWYLCCDTQ MELREWFATF 1150 1160 1170 1180 1190 LFVQHDGLVW PSEPSRVSRA VPEVRLGSVS LIPLRGSENE MRRSVAAFTA DPLSLLRNV
	Isoform 3 [UniParc]. Checksum: A46A522E287CC2C3	FASTA	1,439	160,994
	10 20 30 40 50 60 MAEAGDAALS VAEWLRALHL EQYTGLFEQH GLVWATECQG LSDTRLMDMG MLLPGHRRRI 70 80 90 100 110 120 LAGLLRAHTS PAPAPRPTPR PVPMKRHIFR SPPVPATPPE PLPTTTEDEG LPAAPPIPPR 130 140 150 160 170 180 RSCLPPTCFT TPSTAAPDPV LPPLPAKRHL AELSVPPVPP RTGPPRLLVS LPTKEEESLL 190 200 210 220 230 240 PSLSSPPQPQ SEEPLSTLPQ GPPQPPSPPP CPPEIPPKPV RLFPEFDDSD YDEVPEEGPG 250 260 270 280 290 300 APARVMTKKE EPPPSRVPRA VRVASLLSEG EELSGDDQGD EEEDDHAYEG VPNGGWHTSS 310 320 330 340 350 360 LSLSLPSTIA APHPMDGPPG GSTPVTPVIK AGWLDKNPPQ GSYIYQKRWV RLDTDHLRYF 370 380 390 400 410 420 DSNKDAYSKR FISVACISHV AAIGDQKFEV ITNNRTFAFR AESDVERKEW MQALQQAMAE 430 440 450 460 470 480 QRARARLSSA YLLGVPGSEQ PDRAGSLELR GFKNKLYVAV VGDKVQLYKN LEEYHLGIGI 490 500 510 520 530 540 TFIDMSVGNV KEVDRRSFDL TTPYRIFSFS ADSELEKEQW LEAMQGAIAE ALSTSEVAER 550 560 570 580 590 600 IWAAAPNRFC ADCGAPQPDW ASINLCVVIC KRCAGEHRGL GAGVSKVRSL KMDRKVWTET 610 620 630 640 650 660 LIELFLQLGN GAGNRFWAAN VPPSEALQPS SSPSTRRCHL EAKYREGKYR RYHPLFGNQE 670 680 690 700 710 720 ELDKALCAAV TTTDLAETQA LLGCGAGINC FSGDPEAPTP LALAEQAGQT LQMEFLRNNR 730 740 750 760 770 780 TTEVPRLDSM KPLEKHYSVV LPTVSHSGFL YKTASAGKLL QDRRAREEFS RRWCVLGDGV 790 800 810 820 830 840 LSYFENERAV TPNGEIRASE IVCLAVPPPD THGFEHTFEV YTEGERLYLF GLESAEQAHE 850 860 870 880 890 900 WVKCIAKAFV PPLAEDLLAR DFERLGRLPY KAGLSLQRAQ EGWFSLSGSE LRAVFPEGPC 910 920 930 940 950 960 EEPLQLRKLQ ELSIQGDSEN QVLVLVERRR TLYIQGERRL DFMGWLGAIQ KAAASMGDTL 970 980 990 1000 1010 1020 SEQQLGDSDI PVIVYRCVDY ITQCGLTSEG IYRKCGQTSK TQRLLESLRQ DARSVHLKEG 1030 1040 1050 1060 1070 1080 EQHVDDVSSA LKRFLRDLPD GLFTRAQRLT WLEASEIEDE EEKVSRYREL LVRLPPVNRA 1090 1100 1110 1120 1130 1140 TVKALISHLY CVQCFSDTNQ MNVHNLAIVF GPTLFQTDGQ DYKAGRVVED LINHYVVVFS 1150 1160 1170 1180 1190 1200 VDEEELRKQR EEITAIVKMR VAGTASGTQH AGDFICTVYL EEKKAETEQH IKVPASMTAE 1210 1220 1230 1240 1250 1260 ELTLEILDRR NVGIREKDYW TCFEVNEREE AERPLHFAEK VLPILHGLGT DSHLVVKKHQ 1270 1280 1290 1300 1310 1320 AMEAMLLYLA SRVGDTKHGM MKFREDRSLL GLGLPSGGFH DRYFILNSSC LRLYKEVRSH 1330 1340 1350 1360 1370 1380 RPEKEWPIKS LKVYLGVKKK LRPPTCWGFT VVHETEKHEK QQWYLCCDTQ MELREWFATF 1390 1400 1410 1420 1430 LFVQHDGLVW PSEPSRVSRA VPEVRLGSVS LIPLRGSENE MRRSVAAFTA DPLSLLRNV
	Isoform 4 [UniParc]. Checksum: A679C6A273660197	FASTA	1,205	135,834
	10 20 30 40 50 60 MTKKEEPPPS RVPRAVRVAS LLSEGEELSG DDQGDEEEDD HAYEGVPNGG WHTSSLSLSL 70 80 90 100 110 120 PSTIAAPHPM DGPPGGSTPV TPVIKAGWLD KNPPQGSYIY QKRWVRLDTD HLRYFDSNKD 130 140 150 160 170 180 AYSKRFISVA CISHVAAIGD QKFEVITNNR TFAFRAESDV ERKEWMQALQ QAMAEQRARA 190 200 210 220 230 240 RLSSAYLLGV PGSEQPDRAG SLELRGFKNK LYVAVVGDKV QLYKNLEEYH LGIGITFIDM 250 260 270 280 290 300 SVGNVKEVDR RSFDLTTPYR IFSFSADSEL EKEQWLEAMQ GAIAEALSTS EVAERIWAAA 310 320 330 340 350 360 PNRFCADCGA PQPDWASINL CVVICKRCAG EHRGLGAGVS KVRSLKMDRK VWTETLIELF 370 380 390 400 410 420 LQLGNGAGNR FWAANVPPSE ALQPSSSPST RRCHLEAKYR EGKYRRYHPL FGNQEELDKA 430 440 450 460 470 480 LCAAVTTTDL AETQALLGCG AGINCFSGDP EAPTPLALAE QAGQTLQMEF LRNNRTTEVP 490 500 510 520 530 540 RLDSMKPLEK HYSVVLPTVS HSGFLYKTAS AGKLLQDRRA REEFSRRWCV LGDGVLSYFE 550 560 570 580 590 600 NERAVTPNGE IRASEIVCLA VPPPDTHGFE HTFEVYTEGE RLYLFGLESA EQAHEWVKCI 610 620 630 640 650 660 AKAFVPPLAE DLLARDFERL GRLPYKAGLS LQRAQEGWFS LSGSELRAVF PEGPCEEPLQ 670 680 690 700 710 720 LRKLQELSIQ GDSENQVLVL VERRRTLYIQ GERRLDFMGW LGAIQKAAAS MGDTLSEQQL 730 740 750 760 770 780 GDSDIPVIVY RCVDYITQCG LTSEGIYRKC GQTSKTQRLL ESLRQDARSV HLKEGEQHVD 790 800 810 820 830 840 DVSSALKRFL RDLPDGLFTR AQRLTWLEAS EIEDEEEKVS RYRELLVRLP PVNRATVKAL 850 860 870 880 890 900 ISHLYCVQCF SDTNQMNVHN LAIVFGPTLF QTDGQDYKAG RVVEDLINHY VVVFSVDEEE 910 920 930 940 950 960 LRKQREEITA IVKMRVAGTA SGTQHAGDFI CTVYLEEKKA ETEQHIKVPA SMTAEELTLE 970 980 990 1000 1010 1020 ILDRRNVGIR EKDYWTCFEV NEREEAERPL HFAEKVLPIL HGLGTDSHLV VKKHQAMEAM 1030 1040 1050 1060 1070 1080 LLYLASRVGD TKHGMMKFRE DRSLLGLGLP SGGFHDRYFI LNSSCLRLYK EVRSQRPWSG 1090 1100 1110 1120 1130 1140 APETSHRPEK EWPIKSLKVY LGVKKKLRPP TCWGFTVVHE TEKHEKQQWY LCCDTQMELR 1150 1160 1170 1180 1190 1200 EWFATFLFVQ HDGLVWPSEP SRVSRAVPEV RLGSVSLIPL RGSENEMRRS VAAFTADPLS LLRNV
	Isoform 5 [UniParc]. Checksum: 2F50EB6053978B37	FASTA	679	77,550
	10 20 30 40 50 60 MDASGKGEFS RRWCVLGDGV LSYFENERAV TPNGEIRASE IVCLAVPPPD THGFEHTFEV 70 80 90 100 110 120 YTEGERLYLF GLESAEQAHE WVKCIAKAFV PPLAEDLLAR DFERLGRLPY KAGLSLQRAQ 130 140 150 160 170 180 EGWFSLSGSE LRAVFPEGPC EEPLQLRKLQ ELSIQGDSEN QVLVLVERRR TLYIQGERRL 190 200 210 220 230 240 DFMGWLGAIQ KAAASMGDTL SEQQLGDSDI PVIVYRCVDY ITQCGLTSEG IYRKCGQTSK 250 260 270 280 290 300 TQRLLESLRQ DARSVHLKEG EQHVDDVSSA LKRFLRDLPD GLFTRAQRLT WLEASEIEDE 310 320 330 340 350 360 EEKVSRYREL LVRLPPVNRA TVKALISHLY CVQCFSDTNQ MNVHNLAIVF GPTLFQTDGQ 370 380 390 400 410 420 DYKAGRVVED LINHYVVVFS VDEEELRKQR EEITAIVKMR VAGTASGTQH AGDFICTVYL 430 440 450 460 470 480 EEKKAETEQH IKVPASMTAE ELTLEILDRR NVGIREKDYW TCFEVNEREE AERPLHFAEK 490 500 510 520 530 540 VLPILHGLGT DSHLVVKKHQ AMEAMLLYLA SRVGDTKHGM MKFREDRSLL GLGLPSGGFH 550 560 570 580 590 600 DRYFILNSSC LRLYKEVRSH RPEKEWPIKS LKVYLGVKKK LRPPTCWGFT VVHETEKHEK 610 620 630 640 650 660 QQWYLCCDTQ MELREWFATF LFVQHDGLVW PSEPSRVSRA VPEVRLGSVS LIPLRGSENE 670 MRRSVAAFTA DPLSLLRNV
	Isoform 7 [UniParc]. Checksum: 69A2D13CDDA77185	FASTA	1,133	127,665
	10 20 30 40 50 60 MTKKEEPPPS RVPRAVRVAS LLSEGEELSG DDQGDEEEDD HAYEGVPNGG WHTSSLSLSL 70 80 90 100 110 120 PSTIAAPHPM DGPPGGSTPV TPVIKAGWLD KNPPQGSYIY QKRWVRLDTD HLRYFDSNKD 130 140 150 160 170 180 AYSKRFISVA CISHVAAIGD QKFEVITNNR TFAFRAESDV ERKEWMQALQ QAMAEQRARA 190 200 210 220 230 240 RLSSAYLLGV PGSEQPDRAG SLELRGFKNK LYVAVVGDKV QLYKNLEEYH LGIGITFIDM 250 260 270 280 290 300 SVGNVKEVDR RSFDLTTPYR IFSFSADSEL EKEQWLEAMQ GAIAEALSTS EVAERIWAAA 310 320 330 340 350 360 PNRFCADCGA PQPDWASINL CVVICKRCAG EHRGLGAGVS KVRSLKMDRK VWTETLIEAL 370 380 390 400 410 420 CAAVTTTDLA ETQALLGCGA GINCFSGDPE APTPLALAEQ AGQTLQMEFL RNNRTTEVPR 430 440 450 460 470 480 LDSMKPLEKH YSVVLPTVSH SGFLYKTASA GKLLQDRRAR EEFSRRWCVL GDGVLSYFEN 490 500 510 520 530 540 ERAVTPNGEI RASEIVCLAV PPPDTHGFEH TFEVYTEGER LYLFGLESAE QAHEWVKCIA 550 560 570 580 590 600 KAFVPPLAED LLARDFERLG RLPYKAGLSL QRAQEGWFSL SGSELRAVFP EGPCEEPLQL 610 620 630 640 650 660 RKLQELSIQG DSENQVLVLV ERRRTLYIQG ERRLDFMGWL GAIQKAAASM GDTLSEQQLG 670 680 690 700 710 720 DSDIPVIVYR CVDYITQCGL TSEGIYRKCG QTSKTQRLLE SLRQDARSVH LKEGEQHVDD 730 740 750 760 770 780 VSSALKRFLR DLPDGLFTRA QRLTWLEASE IEDEEEKVSR YRELLVRLPP VNRATVKALI 790 800 810 820 830 840 SHLYCVQCFS DTNQMNVHNL AIVFGPTLFQ TDGQDYKAGR VVEDLINHYV VVFSVDEEEL 850 860 870 880 890 900 RKQREEITAI VKMRVAGTAS GTQHAGDFIC TVYLEEKKAE TEQHIKVPAS MTAEELTLEI 910 920 930 940 950 960 LDRRNVGIRE KDYWTCFEVN EREEAERPLH FAEKVLPILH GLGTDSHLVV KKHQAMEAML 970 980 990 1000 1010 1020 LYLASRVGDT KHGMMKFRED RSLLGLGLPS GGFHDRYFIL NSSCLRLYKE VRSHRPEKEW 1030 1040 1050 1060 1070 1080 PIKSLKVYLG VKKKLRPPTC WGFTVVHETE KHEKQQWYLC CDTQMELREW FATFLFVQHD 1090 1100 1110 1120 1130 GLVWPSEPSR VSRAVPEVRL GSVSLIPLRG SENEMRRSVA AFTADPLSLL RNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ARAP1: a point of convergence for Arf and Rho signaling." Miura K., Jacques K.M., Stauffer S., Kubosaki A., Zhu K., Hirsch D.S., Resau J., Zheng Y., Randazzo P.A. Mol. Cell 9:109-119(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"ARAP1 splice variants in man and mouse." Krugmann S., Coadwell J., Stephens L.R., Hawkins P.T. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
[3]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Human ARAP1b." Randazzo P.A., Yoon H.-Y., Miura K. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT GLU-1047. Tissue: Brain.
[7]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 7), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 792-1450 (ISOFORMS 2/3/5). Tissue: Brain and Pancreas.
[9]	"KIAA0782 as a member (centaurin delta2) of the ArfGAP centaurin family." Hong W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-1450 (ISOFORM 1).
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-354, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." Simova S., Klima M., Cermak L., Sourkova V., Andera L. Apoptosis 13:423-436(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TNFRSF10A, SUBCELLULAR LOCATION.
[12]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229; TYR-431 AND SER-1435, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-738 AND SER-1435, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY049732 mRNA. Translation: AAL12169.1. AJ621557 mRNA. Translation: CAF21317.1. AB018325 mRNA. Translation: BAA34502.2. Different initiation. AY553630 mRNA. Translation: AAT36325.1. AB209473 mRNA. Translation: BAD92710.1. Different initiation. AP002381 Genomic DNA. No translation available. AP003065 Genomic DNA. No translation available. BC008315 mRNA. Translation: AAH08315.1. BC021244 mRNA. No translation available. BC056401 mRNA. Translation: AAH56401.1. BC140792 mRNA. Translation: AAI40793.1. AF411983 mRNA. Translation: AAL04167.1.
IPI	IPI00220421. IPI00479190. IPI00639996. IPI00640351. IPI00646915. IPI00828080.
PIR	C59431.
RefSeq	NP_001035207.1. NM_001040118.2. NP_001128662.1. NM_001135190.1. NP_056057.2. NM_015242.4.
UniGene	Hs.503165.
3D structure databases
ProteinModelPortal	Q96P48.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96P48. 9 interactions.
MINT	MINT-1395507.
Polymorphism databases
DMDM	226694321.
Proteomic databases
PaxDb	Q96P48.
PRIDE	Q96P48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334211; ENSP00000335506; ENSG00000186635. ENST00000359373; ENSP00000352332; ENSG00000186635. ENST00000393605; ENSP00000377230; ENSG00000186635. ENST00000393609; ENSP00000377233; ENSG00000186635. ENST00000429686; ENSP00000403127; ENSG00000186635. ENST00000455638; ENSP00000390461; ENSG00000186635.
GeneID	116985.
KEGG	hsa:116985.
UCSC	uc001osr.3. human. uc001oss.3. human. uc001osv.3. human.
Organism-specific databases
CTD	116985.
GeneCards	GC11M072396.
H-InvDB	HIX0079401. HIX0171326.
HGNC	HGNC:16925. ARAP1.
HPA	CAB020703.
MIM	606646. gene.
neXtProt	NX_Q96P48.
PharmGKB	PA164715867.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5347.
HOGENOM	HOG000246988.
HOVERGEN	HBG106625.
KO	K12490.
OMA	VKCIAKA.
OrthoDB	EOG4HMJ8F.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q96P48.
Bgee	Q96P48.
CleanEx	HS_ARAP1.
Genevestigator	Q96P48.
GermOnline	ENSG00000186635. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.50. 1 hit. 1.10.555.10. 1 hit. 2.30.29.30. 4 hits.
InterPro	IPR001164. ArfGAP. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000159. Ras-assoc. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Pfam	PF01412. ArfGap. 1 hit. PF00169. PH. 3 hits. PF00788. RA. 1 hit. PF00620. RhoGAP. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00105. ArfGap. 1 hit. SM00233. PH. 5 hits. SM00324. RhoGAP. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit. SSF47769. SAM_homology. 1 hit.
PROSITE	PS50115. ARFGAP. 1 hit. PS50003. PH_DOMAIN. 4 hits. PS50200. RA. 1 hit. PS50238. RHOGAP. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ARAP1. human.
GenomeRNAi	116985.
NextBio	80062.
SOURCE	Search...

Entry information

Entry name

ARAP1_HUMAN

Accession

Primary (citable) accession number: Q96P48
Secondary accession number(s): A3KLL7 , B2RTS2, O94879, Q4LDD5, Q59FI7, Q6PHS3, Q8WU51, Q96HP6, Q96L71

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.