Reviewed, UniProtKB/Swiss-Prot Q96P47 (AGAP3_HUMAN)

Names and origin

Protein names

Recommended name:
    Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
      Short name=AGAP-3
Alternative name(s):
    Centaurin-gamma-3
    MR1-interacting protein
    MRIP-1
    CRAM-associated GTPase
      Short name=CRAG

Gene names

Name:	AGAP3
Synonyms:	CENTG3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	875 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase-activating protein for the ADP ribosylation factor family Potential. GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway.
Enzyme regulation	GTPase activity is stimulated by oxidative stress. Ref.5
Subunit structure	Interacts with PML. Interacts with expanded polyglutamine proteins. Ref.5
Subcellular location	Cytoplasm. Note: In cells upon oxidative stress or in brains of Machado-Joseph disease patients, translocates to PML nuclear bodies. Ref.5
Tissue specificity	Widely expressed. Ref.5 Ref.4
Sequence similarities	Belongs to the centaurin gamma-like family. Contains 3 ANK repeats. Contains 1 Arf-GAP domain. Contains 1 PH domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Zinc-finger
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96P47-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96P47-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MNFQ → MFGGAGPG
     9-15: QSPQQQQ → GPSQ

Isoform 3 (identifier: Q96P47-3)

The sequence of this isoform differs from the canonical sequence as follows:
     341-360: SRKGADLDREKKAAECKVDS → ICATVSNFSSTKRPFQLLPN
     361-875: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 875	875	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3		PRO_0000074220
Regions
	Domain	367 – 605	239	PH
	Domain	626 – 746	121	Arf-GAP
	Repeat	748 – 777	30	ANK 1
	Repeat	785 – 814	30	ANK 2
	Repeat	818 – 847	30	ANK 3
	Nucleotide binding	98 – 105	8	GTP Potential
	Nucleotide binding	142 – 146	5	GTP Potential
	Nucleotide binding	198 – 201	4	GTP Potential
	Zinc finger	641 – 664	24	C4-type
	Region	84 – 375	292	Small GTPase-like
Amino acid modifications
	Modified residue	324	1	Phosphothreonine Ref.7
	Modified residue	326	1	Phosphothreonine Ref.7
	Modified residue	336	1	Phosphoserine Ref.7
	Modified residue	443	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 4	4	MNFQ → MFGGAGPG in isoform 2.		VSP_018534
	Alternative sequence	9 – 15	7	QSPQQQQ → GPSQ in isoform 2.		VSP_018535
	Alternative sequence	341 – 360	20	SRKGA…CKVDS → ICATVSNFSSTKRPFQLLPN in isoform 3.		VSP_018536
	Alternative sequence	361 – 875	515	Missing in isoform 3.		VSP_018537

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 3F0D04EE00F42E1E	FASTA	875	95,044
	10 20 30 40 50 60 MNFQAGGGQS PQQQQQLAGG PPQQFALSNS AAIRAEIQRF ESVHPNIYAI YDLIERIEDL 70 80 90 100 110 120 ALQNQIREHV ISIEDSFVNS QEWTLSRSVP ELKVGIVGNL SSGKSALVHR YLTGTYVQEE 130 140 150 160 170 180 SPEGGRFKKE IVVDGQSYLL LIRDEGGPPE LQFAAWVDAV VFVFSLEDEI SFQTVYNYFL 190 200 210 220 230 240 RLCSFRNASE VPMVLVGTQD AISAANPRVI DDSRARKLST DLKRCTYYET CATYGLNVER 250 260 270 280 290 300 VFQDVAQKVV ALRKKQQLAI GPCKSLPNSP SHSAVSAASI PAVHINQATN GGGSAFSDYS 310 320 330 340 350 360 SSVPSTPSIS QRELRIETIA ASSTPTPIRK QSKRRSNIFT SRKGADLDRE KKAAECKVDS 370 380 390 400 410 420 IGSGRAIPIK QGILLKRSGK SLNKEWKKKY VTLCDNGLLT YHPSLHDYMQ NIHGKEIDLL 430 440 450 460 470 480 RTTVKVPGKR LPRATPATAP GTSPRANGLS VERSNTQLGG GTGAPHSASS ASLHSERPLS 490 500 510 520 530 540 SSAWAGPRPE GLHQRSCSVS SADQWSEATT SLPPGMQHPA SGPAEVLSSS PKLDPPPSPH 550 560 570 580 590 600 SNRKKHRRKK STGTPRPDGP SSATEEAEES FEFVVVSLTG QTWHFEASTA EERELWVQSV 610 620 630 640 650 660 QAQILASLQG CRSAKDKTRL GNQNAALAVQ AVRTVRGNSF CIDCDAPNPD WASLNLGALM 670 680 690 700 710 720 CIECSGIHRH LGAHLSRVRS LDLDDWPPEL LAVMTAMGNA LANSVWEGAL GGYSKPGPDA 730 740 750 760 770 780 CREEKERWIR AKYEQKLFLA PLPSSDVPLG QQLLRAVVED DLRLLVMLLA HGSKEEVNET 790 800 810 820 830 840 YGDGDGRTAL HLSSAMANVV FTQLLIWYGV DVRSRDARGL TPLAYARRAG SQECADILIQ 850 860 870 HGCPGEGCGL APTPNREPAN GTNPSAELHR SPSLL
	Isoform 2. Checksum: A068C38000F6F33E	FASTA	876	94,743
	10 20 30 40 50 60 MFGGAGPGAG GGGPSQQLAG GPPQQFALSN SAAIRAEIQR FESVHPNIYA IYDLIERIED 70 80 90 100 110 120 LALQNQIREH VISIEDSFVN SQEWTLSRSV PELKVGIVGN LSSGKSALVH RYLTGTYVQE 130 140 150 160 170 180 ESPEGGRFKK EIVVDGQSYL LLIRDEGGPP ELQFAAWVDA VVFVFSLEDE ISFQTVYNYF 190 200 210 220 230 240 LRLCSFRNAS EVPMVLVGTQ DAISAANPRV IDDSRARKLS TDLKRCTYYE TCATYGLNVE 250 260 270 280 290 300 RVFQDVAQKV VALRKKQQLA IGPCKSLPNS PSHSAVSAAS IPAVHINQAT NGGGSAFSDY 310 320 330 340 350 360 SSSVPSTPSI SQRELRIETI AASSTPTPIR KQSKRRSNIF TSRKGADLDR EKKAAECKVD 370 380 390 400 410 420 SIGSGRAIPI KQGILLKRSG KSLNKEWKKK YVTLCDNGLL TYHPSLHDYM QNIHGKEIDL 430 440 450 460 470 480 LRTTVKVPGK RLPRATPATA PGTSPRANGL SVERSNTQLG GGTGAPHSAS SASLHSERPL 490 500 510 520 530 540 SSSAWAGPRP EGLHQRSCSV SSADQWSEAT TSLPPGMQHP ASGPAEVLSS SPKLDPPPSP 550 560 570 580 590 600 HSNRKKHRRK KSTGTPRPDG PSSATEEAEE SFEFVVVSLT GQTWHFEAST AEERELWVQS 610 620 630 640 650 660 VQAQILASLQ GCRSAKDKTR LGNQNAALAV QAVRTVRGNS FCIDCDAPNP DWASLNLGAL 670 680 690 700 710 720 MCIECSGIHR HLGAHLSRVR SLDLDDWPPE LLAVMTAMGN ALANSVWEGA LGGYSKPGPD 730 740 750 760 770 780 ACREEKERWI RAKYEQKLFL APLPSSDVPL GQQLLRAVVE DDLRLLVMLL AHGSKEEVNE 790 800 810 820 830 840 TYGDGDGRTA LHLSSAMANV VFTQLLIWYG VDVRSRDARG LTPLAYARRA GSQECADILI 850 860 870 QHGCPGEGCG LAPTPNREPA NGTNPSAELH RSPSLL
	Isoform 3. Checksum: D9D68897C6E2C0DD	FASTA	360	39,610
	10 20 30 40 50 60 MNFQAGGGQS PQQQQQLAGG PPQQFALSNS AAIRAEIQRF ESVHPNIYAI YDLIERIEDL 70 80 90 100 110 120 ALQNQIREHV ISIEDSFVNS QEWTLSRSVP ELKVGIVGNL SSGKSALVHR YLTGTYVQEE 130 140 150 160 170 180 SPEGGRFKKE IVVDGQSYLL LIRDEGGPPE LQFAAWVDAV VFVFSLEDEI SFQTVYNYFL 190 200 210 220 230 240 RLCSFRNASE VPMVLVGTQD AISAANPRVI DDSRARKLST DLKRCTYYET CATYGLNVER 250 260 270 280 290 300 VFQDVAQKVV ALRKKQQLAI GPCKSLPNSP SHSAVSAASI PAVHINQATN GGGSAFSDYS 310 320 330 340 350 360 SSVPSTPSIS QRELRIETIA ASSTPTPIRK QSKRRSNIFT ICATVSNFSS TKRPFQLLPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins." Xia C., Ma W., Stafford L.J., Liu C., Gong L., Martin J.F., Liu M. Mol. Cell. Biol. 23:2476-2488(2003) [PubMed: 12640130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart.
[2]	"MRIP-1 as a member (centaurin gamma3) of the centaurin ArfGAP protein family." Hong W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 45-855 (ISOFORM 3). Tissue: Brain.
[4]	"AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase." Meurer S., Pioch S., Wagner K., Mueller-Esterl W., Gross S. J. Biol. Chem. 279:49346-49354(2004) [PubMed: 15381706] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein." Qin Q., Inatome R., Hotta A., Kojima M., Yamamura H., Hirai H., Yoshizawa T., Tanaka H., Fukami K., Yanagi S. J. Cell Biol. 172:497-504(2006) [PubMed: 16461359] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PML, ENZYME REGULATION, FUNCTION.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-324; THR-326 AND SER-336, MASS SPECTROMETRY.
[8]	"Crystal structure of the Ras-like domain of CENTG3." Structural genomics consortium (SGC) Submitted (AUG-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 90-255.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF359283 mRNA. Translation: AAK48932.2.
AF413079 mRNA. Translation: AAL04173.1.
AB209781 mRNA. Translation: BAD93018.1.

IPI

IPI00163185.
IPI00479742.
IPI00747315.

RefSeq

NP_001036000.1.
NP_114152.3.

UniGene

Hs.647075

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IHW	X-ray	1.92	A	90-255	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q96P47.

PTM databases

PhosphoSite

Q96P47.

Proteomic databases

PRIDE

Q96P47.

Genome annotation databases

Ensembl

ENST00000335355; ENSP00000334157; ENSG00000133612; Homo sapiens. [Genome view]

GeneID

116988.

KEGG

hsa:116988.

UCSC

uc003wjf.1. human.
uc003wjg.1. human.

Organism-specific databases

CTD

116988.

GeneCards

GC07P150415.

HGNC

HGNC:16923. AGAP3.

HPA

HPA012883.

PharmGKB

PA26413.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q96P47.

HOVERGEN

Q96P47.

Enzyme and pathway databases

BioCyc

CATTLE:ENSBTAG00000008468-MON.

Gene expression databases

ArrayExpress

Q96P47.

Bgee

Q96P47.

CleanEx

HS_AGAP3.

Genevestigator

Q96P47.

GermOnline

ENSG00000133612. Homo sapiens.

Family and domain databases

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR001849. Pleckstrin_homology.
IPR020849. Ras_small_GTPase.
[Graphical view]

Gene3D

G3DSA:1.25.40.20. ANK. 1 hit.

Pfam

PF00023. Ank. 2 hits.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]

PRINTS

PR00405. REVINTRACTNG.

SMART

SM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51421. RAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

80080.

Entry information

Entry name

AGAP3_HUMAN

Accession

Primary (citable) accession number: Q96P47
Secondary accession number(s): Q59EN0, Q96RK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.