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Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

Gene

AGAP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family (Potential). GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway.Curated1 Publication

Enzyme regulationi

GTPase activity is stimulated by oxidative stress.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 105GTPSequence analysis8
Nucleotide bindingi142 – 146GTPSequence analysis5
Nucleotide bindingi198 – 201GTPSequence analysis4
Zinc fingeri641 – 664C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133612-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
Short name:
AGAP-3
Alternative name(s):
CRAM-associated GTPase
Short name:
CRAG
Centaurin-gamma-3
Short name:
Cnt-g3
MR1-interacting protein
Short name:
MRIP-1
Gene namesi
Name:AGAP3
Synonyms:CENTG3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:16923. AGAP3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000133612.
PharmGKBiPA26413.

Polymorphism and mutation databases

DMDMi97535922.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742201 – 875Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei324PhosphothreonineCombined sources1
Modified residuei326PhosphothreonineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei538PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96P47.
MaxQBiQ96P47.
PaxDbiQ96P47.
PeptideAtlasiQ96P47.
PRIDEiQ96P47.

PTM databases

iPTMnetiQ96P47.
PhosphoSitePlusiQ96P47.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000133612.
CleanExiHS_AGAP3.
ExpressionAtlasiQ96P47. baseline and differential.
GenevisibleiQ96P47. HS.

Organism-specific databases

HPAiHPA012883.

Interactioni

Subunit structurei

Interacts with PML. Interacts with expanded polyglutamine proteins.1 Publication

Protein-protein interaction databases

BioGridi125551. 12 interactors.
IntActiQ96P47. 3 interactors.
STRINGi9606.ENSP00000380413.

Structurei

Secondary structure

1875
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi91 – 97Combined sources7
Helixi104 – 113Combined sources10
Beta strandi125 – 133Combined sources9
Beta strandi136 – 144Combined sources9
Beta strandi146 – 148Combined sources3
Helixi151 – 156Combined sources6
Beta strandi158 – 165Combined sources8
Helixi169 – 183Combined sources15
Helixi188 – 190Combined sources3
Beta strandi193 – 198Combined sources6
Helixi212 – 221Combined sources10
Turni222 – 224Combined sources3
Beta strandi226 – 231Combined sources6
Turni232 – 235Combined sources4
Helixi238 – 252Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IHWX-ray1.92A90-255[»]
ProteinModelPortaliQ96P47.
SMRiQ96P47.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96P47.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini367 – 605PHPROSITE-ProRule annotationAdd BLAST239
Domaini626 – 746Arf-GAPPROSITE-ProRule annotationAdd BLAST121
Repeati748 – 777ANK 1Add BLAST30
Repeati785 – 814ANK 2Add BLAST30
Repeati818 – 847ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 375Small GTPase-likeAdd BLAST292

Sequence similaritiesi

Belongs to the centaurin gamma-like family.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri641 – 664C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0705. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000007233.
HOVERGENiHBG054045.
InParanoidiQ96P47.
KOiK12491.
OMAiDQWSEAA.
OrthoDBiEOG091G123A.
PhylomeDBiQ96P47.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51421. RAS. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96P47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFQAGGGQS PQQQQQLAGG PPQQFALSNS AAIRAEIQRF ESVHPNIYAI
60 70 80 90 100
YDLIERIEDL ALQNQIREHV ISIEDSFVNS QEWTLSRSVP ELKVGIVGNL
110 120 130 140 150
SSGKSALVHR YLTGTYVQEE SPEGGRFKKE IVVDGQSYLL LIRDEGGPPE
160 170 180 190 200
LQFAAWVDAV VFVFSLEDEI SFQTVYNYFL RLCSFRNASE VPMVLVGTQD
210 220 230 240 250
AISAANPRVI DDSRARKLST DLKRCTYYET CATYGLNVER VFQDVAQKVV
260 270 280 290 300
ALRKKQQLAI GPCKSLPNSP SHSAVSAASI PAVHINQATN GGGSAFSDYS
310 320 330 340 350
SSVPSTPSIS QRELRIETIA ASSTPTPIRK QSKRRSNIFT SRKGADLDRE
360 370 380 390 400
KKAAECKVDS IGSGRAIPIK QGILLKRSGK SLNKEWKKKY VTLCDNGLLT
410 420 430 440 450
YHPSLHDYMQ NIHGKEIDLL RTTVKVPGKR LPRATPATAP GTSPRANGLS
460 470 480 490 500
VERSNTQLGG GTGAPHSASS ASLHSERPLS SSAWAGPRPE GLHQRSCSVS
510 520 530 540 550
SADQWSEATT SLPPGMQHPA SGPAEVLSSS PKLDPPPSPH SNRKKHRRKK
560 570 580 590 600
STGTPRPDGP SSATEEAEES FEFVVVSLTG QTWHFEASTA EERELWVQSV
610 620 630 640 650
QAQILASLQG CRSAKDKTRL GNQNAALAVQ AVRTVRGNSF CIDCDAPNPD
660 670 680 690 700
WASLNLGALM CIECSGIHRH LGAHLSRVRS LDLDDWPPEL LAVMTAMGNA
710 720 730 740 750
LANSVWEGAL GGYSKPGPDA CREEKERWIR AKYEQKLFLA PLPSSDVPLG
760 770 780 790 800
QQLLRAVVED DLRLLVMLLA HGSKEEVNET YGDGDGRTAL HLSSAMANVV
810 820 830 840 850
FTQLLIWYGV DVRSRDARGL TPLAYARRAG SQECADILIQ HGCPGEGCGL
860 870
APTPNREPAN GTNPSAELHR SPSLL
Length:875
Mass (Da):95,044
Last modified:May 16, 2006 - v2
Checksum:i3F0D04EE00F42E1E
GO
Isoform 2 (identifier: Q96P47-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MNFQ → MFGGAGPG
     9-15: QSPQQQQ → GPSQ

Show »
Length:876
Mass (Da):94,743
Checksum:iA068C38000F6F33E
GO
Isoform 3 (identifier: Q96P47-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     341-360: SRKGADLDREKKAAECKVDS → ICATVSNFSSTKRPFQLLPN
     361-875: Missing.

Show »
Length:360
Mass (Da):39,610
Checksum:iD9D68897C6E2C0DD
GO
Isoform 4 (identifier: Q96P47-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-15: Q → QSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGGPSQ

Show »
Length:911
Mass (Da):97,951
Checksum:i9D5B0058D20EB3DC
GO
Isoform 5 (identifier: Q96P47-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.
     463-565: Missing.

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):62,846
Checksum:i26A2BBC97484F5C6
GO
Isoform 6 (identifier: Q96P47-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-15: Q → QSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGGPSQ
     341-360: SRKGADLDREKKAAECKVDS → ICATVSNFSSTKRPFQLLPN
     361-875: Missing.

Show »
Length:396
Mass (Da):42,517
Checksum:i2AB030A89989B7C4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0548901 – 192Missing in isoform 5. 1 PublicationAdd BLAST192
Alternative sequenceiVSP_0185341 – 4MNFQ → MFGGAGPG in isoform 2. 1 Publication4
Alternative sequenceiVSP_0185359 – 15QSPQQQQ → GPSQ in isoform 2. 1 Publication7
Alternative sequenceiVSP_04037315Q → QSLAAPGGGGAAAQQLVCGG QFGGAGPGAGGGGGPSQ in isoform 4 and isoform 6. Curated1
Alternative sequenceiVSP_018536341 – 360SRKGA…CKVDS → ICATVSNFSSTKRPFQLLPN in isoform 3 and isoform 6. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_018537361 – 875Missing in isoform 3 and isoform 6. 1 PublicationAdd BLAST515
Alternative sequenceiVSP_054891463 – 565Missing in isoform 5. 1 PublicationAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF359283 mRNA. Translation: AAK48932.2.
AF413079 mRNA. Translation: AAL04173.1.
AK055393 mRNA. Translation: BAG51510.1.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54026.1.
CH471173 Genomic DNA. Translation: EAW54028.1.
AB209781 mRNA. Translation: BAD93018.1.
CCDSiCCDS43681.1. [Q96P47-4]
CCDS55185.1. [Q96P47-6]
CCDS64802.1. [Q96P47-5]
RefSeqiNP_001036000.1. NM_001042535.3. [Q96P47-6]
NP_001268229.1. NM_001281300.1. [Q96P47-5]
NP_114152.3. NM_031946.6. [Q96P47-4]
XP_016867223.1. XM_017011734.1. [Q96P47-5]
UniGeneiHs.647075.

Genome annotation databases

EnsembliENST00000397238; ENSP00000380413; ENSG00000133612. [Q96P47-4]
ENST00000463381; ENSP00000418016; ENSG00000133612. [Q96P47-5]
ENST00000473312; ENSP00000418921; ENSG00000133612. [Q96P47-6]
ENST00000622464; ENSP00000480655; ENSG00000133612. [Q96P47-1]
GeneIDi116988.
KEGGihsa:116988.
UCSCiuc003wje.3. human. [Q96P47-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF359283 mRNA. Translation: AAK48932.2.
AF413079 mRNA. Translation: AAL04173.1.
AK055393 mRNA. Translation: BAG51510.1.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54026.1.
CH471173 Genomic DNA. Translation: EAW54028.1.
AB209781 mRNA. Translation: BAD93018.1.
CCDSiCCDS43681.1. [Q96P47-4]
CCDS55185.1. [Q96P47-6]
CCDS64802.1. [Q96P47-5]
RefSeqiNP_001036000.1. NM_001042535.3. [Q96P47-6]
NP_001268229.1. NM_001281300.1. [Q96P47-5]
NP_114152.3. NM_031946.6. [Q96P47-4]
XP_016867223.1. XM_017011734.1. [Q96P47-5]
UniGeneiHs.647075.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IHWX-ray1.92A90-255[»]
ProteinModelPortaliQ96P47.
SMRiQ96P47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125551. 12 interactors.
IntActiQ96P47. 3 interactors.
STRINGi9606.ENSP00000380413.

PTM databases

iPTMnetiQ96P47.
PhosphoSitePlusiQ96P47.

Polymorphism and mutation databases

DMDMi97535922.

Proteomic databases

EPDiQ96P47.
MaxQBiQ96P47.
PaxDbiQ96P47.
PeptideAtlasiQ96P47.
PRIDEiQ96P47.

Protocols and materials databases

DNASUi116988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397238; ENSP00000380413; ENSG00000133612. [Q96P47-4]
ENST00000463381; ENSP00000418016; ENSG00000133612. [Q96P47-5]
ENST00000473312; ENSP00000418921; ENSG00000133612. [Q96P47-6]
ENST00000622464; ENSP00000480655; ENSG00000133612. [Q96P47-1]
GeneIDi116988.
KEGGihsa:116988.
UCSCiuc003wje.3. human. [Q96P47-1]

Organism-specific databases

CTDi116988.
GeneCardsiAGAP3.
H-InvDBHIX0007225.
HGNCiHGNC:16923. AGAP3.
HPAiHPA012883.
neXtProtiNX_Q96P47.
OpenTargetsiENSG00000133612.
PharmGKBiPA26413.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0705. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000007233.
HOVERGENiHBG054045.
InParanoidiQ96P47.
KOiK12491.
OMAiDQWSEAA.
OrthoDBiEOG091G123A.
PhylomeDBiQ96P47.
TreeFamiTF317762.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133612-MONOMER.

Miscellaneous databases

ChiTaRSiAGAP3. human.
EvolutionaryTraceiQ96P47.
GeneWikiiCENTG3.
GenomeRNAii116988.
PROiQ96P47.

Gene expression databases

BgeeiENSG00000133612.
CleanExiHS_AGAP3.
ExpressionAtlasiQ96P47. baseline and differential.
GenevisibleiQ96P47. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAP3_HUMAN
AccessioniPrimary (citable) accession number: Q96P47
Secondary accession number(s): B3KNZ8
, E9PAL8, Q59EN0, Q96RK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.