Q96P26 (5NT1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic 5'-nucleotidase 1B
Short name=cN1B
EC=3.1.3.5

Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name=cN-IB

Gene names

Name:	NT5C1B
Synonyms:	AIRP
ORF Names:	FKSG85

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by ADP By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in testis, placenta and pancreas. Detected at lower levels in heart, kidney, liver and lung. Ref.8
Sequence similarities	Belongs to the 5'-nucleotidase type 3 family.
Sequence caution	The sequence AAK39108.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK83285.1 differs from that shown. Reason: Frameshift at position 320. The sequence BAC04103.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC44363.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base metabolic process Traceable author statement. Source: Reactome purine nucleotide catabolic process Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96P26-1)

Also known as: CN-Ib alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96P26-2)

The sequence of this isoform differs from the canonical sequence as follows:
     41-100: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96P26-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q96P26-4)

The sequence of this isoform differs from the canonical sequence as follows:
     596-610: LGSIAAYGFNKKFSS → KSLGWMS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	Cytosolic 5'-nucleotidase 1B		PRO_0000144796
Regions
	Compositional bias	102 – 176	75	Ser-rich
	Compositional bias	157 – 201	45	Pro-rich
	Compositional bias	267 – 271	5	Poly-Gln
Sites
	Active site	467	1	Nucleophile By similarity
Natural variations
	Alternative sequence	1 – 208	208	Missing in isoform 3.		VSP_010202
	Alternative sequence	41 – 100	60	Missing in isoform 2.		VSP_010201
	Alternative sequence	596 – 610	15	LGSIA…KKFSS → KSLGWMS in isoform 4.		VSP_010203
Experimental info
	Sequence conflict	173	1	G → R in AAL11910. Ref.2
	Sequence conflict	287	1	R → K in AAK39108. Ref.1
	Sequence conflict	297	1	S → W in AAK39108. Ref.1
	Sequence conflict	351	1	P → Q in AAL11910. Ref.2
	Sequence conflict	362	1	V → I in BAC04174. Ref.3
	Sequence conflict	528	1	L → P in CAC44363. Ref.8
	Sequence conflict	552	1	L → F in AAK39108. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CN-Ib alpha) [UniParc]. Last modified May 10, 2004. Version 2. Checksum: EEC0BE9E5498B3E9	FASTA	610	68,804
	10 20 30 40 50 60 MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS LRCCPFQGHS 70 80 90 100 110 120 SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR GSQESSLRKT DSRGYLVRSQ 130 140 150 160 170 180 WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP 190 200 210 220 230 240 ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP 250 260 270 280 290 300 TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW 310 320 330 340 350 360 PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ 370 380 390 400 410 420 YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK 430 440 450 460 470 480 AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF 490 500 510 520 530 540 TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS 550 560 570 580 590 600 AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA 610 AYGFNKKFSS
	Isoform 2 [UniParc]. Checksum: C0C913137DFD2479	FASTA	550	61,934
	10 20 30 40 50 60 MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ GSQESSLRKT DSRGYLVRSQ 70 80 90 100 110 120 WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP 130 140 150 160 170 180 ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP 190 200 210 220 230 240 TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW 250 260 270 280 290 300 PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ 310 320 330 340 350 360 YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK 370 380 390 400 410 420 AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF 430 440 450 460 470 480 TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS 490 500 510 520 530 540 AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA 550 AYGFNKKFSS
	Isoform 3 [UniParc]. Checksum: BFF1852B61C0EA5B	FASTA	402	45,860
	10 20 30 40 50 60 MQENPEAWAQ GIVREIRQTR DSQPLEYSRT SPTEWKSSSQ RRGIYPASTQ LDRNSLSEQQ 70 80 90 100 110 120 QQQREDEDDY EAAYWASMRS FYEKNPSCSR PWPPKPKNAI TIALSSCALF NMVDGRKIYE 130 140 150 160 170 180 QEGLEKYMEY QLTNENVILT PGPAFRFVKA LQYVNARLRD LYPDEQDLFD IVLMTNNHAQ 190 200 210 220 230 240 VGVRLINSVN HYGLLIDRFC LTGGKDPIGY LKAYLTNLYI AADSEKVQEA IQEGIASATM 250 260 270 280 290 300 FDGAKDMAYC DTQLRVAFDG DAVLFSDESE HFTKEHGLDK FFQYDTLCES KPLAQGPLKG 310 320 330 340 350 360 FLEDLGRLQK KFYAKNERLL CPIRTYLVTA RSAASSGARV LKTLRRWGLE IDEALFLAGA 370 380 390 400 PKSPILVKIR PHIFFDDHMF HIEGAQRLGS IAAYGFNKKF SS
	Isoform 4 [UniParc]. Checksum: 1D2A9C840FE9ED0D	FASTA	602	68,022
	10 20 30 40 50 60 MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS LRCCPFQGHS 70 80 90 100 110 120 SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR GSQESSLRKT DSRGYLVRSQ 130 140 150 160 170 180 WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP 190 200 210 220 230 240 ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP 250 260 270 280 290 300 TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW 310 320 330 340 350 360 PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ 370 380 390 400 410 420 YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK 430 440 450 460 470 480 AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF 490 500 510 520 530 540 TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS 550 560 570 580 590 600 AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRKSLGW MS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel human testis cDNA encoding a polypeptide related to autoimmune infertility." Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"Homo sapiens cytosolic nucleotidase Ib alpha mRNA." Ji Y., Mitchell B.S., Spychala J. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Testis.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	"Identification and characterization of FKSG85 which encodes a nucleotidase protein." Wang Y.-G., Gong L. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 312-610.
[8]	"Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein." Sala-Newby G.B., Newby A.C. Biochim. Biophys. Acta 1521:12-18(2001) [PubMed: 11690631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 325-601, TISSUE SPECIFICITY. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF356185 mRNA. Translation: AAK39108.1. Different initiation. AF417165 mRNA. Translation: AAL11910.1. AK093234 mRNA. Translation: BAC04103.1. Different initiation. AK093464 mRNA. Translation: BAC04174.1. AK057444 mRNA. Translation: BAB71489.1. AC079148 Genomic DNA. Translation: AAX93260.1. CH471053 Genomic DNA. Translation: EAX00853.1. BC136387 mRNA. Translation: AAI36388.1. BC171761 mRNA. Translation: AAI71761.1. AF352326 mRNA. Translation: AAK83285.1. Frameshift. AJ295254 mRNA. Translation: CAC44363.1. Different initiation.
IPI	IPI00169426. IPI00410373. IPI00410375. IPI00892609.
RefSeq	NP_001002006.1. NM_001002006.2. NP_001186015.1. NM_001199086.1. NP_001186016.1. NM_001199087.1. NP_001186017.1. NM_001199088.1. NP_001186033.1. NM_001199104.1. NP_150278.2. NM_033253.3.
UniGene	Hs.120319.
3D structure databases
ProteinModelPortal	Q96P26.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96P26.
PTM databases
PhosphoSite	Q96P26.
Polymorphism databases
DMDM	47116569.
Proteomic databases
PRIDE	Q96P26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359846; ENSP00000352904; ENSG00000185013. ENST00000403770; ENSP00000384831; ENSG00000185013.
GeneID	100526794. 93034.
KEGG	hsa:100526794. hsa:93034.
UCSC	uc002rcy.1. human. uc002rcz.1. human. uc002rda.1. human. uc010exr.1. human.
Organism-specific databases
CTD	100526794. 93034.
GeneCards	GC02M018744.
H-InvDB	HIX0001850.
HGNC	HGNC:17818. NT5C1B.
MIM	610526. gene.
neXtProt	NX_Q96P26.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18906.
GeneTree	ENSGT00390000017767.
HOVERGEN	HBG050426.
InParanoid	Q96P26.
OrthoDB	EOG44XJGN.
PhylomeDB	Q96P26.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q96P26.
Bgee	Q96P26.
CleanEx	HS_NT5C1B.
Genevestigator	Q96P26.
GermOnline	ENSG00000185013. Homo sapiens.
Family and domain databases
InterPro	IPR010394. 5-nucleotidase. [Graphical view]
KO	K01081.
Pfam	PF06189. 5-nucleotidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	77947.
SOURCE	Search...

Entry information

Entry name

5NT1B_HUMAN

Accession

Primary (citable) accession number: Q96P26
Secondary accession number(s): B5MCR0 , B7ZVX7, Q53RX2, Q8N9W3, Q8NA26, Q96DU5, Q96KE6, Q96M25, Q96SA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.