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Protein

Cytosolic 5'-nucleotidase 1B

Gene

NT5C1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels (By similarity).By similarity

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by ADP.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei467 – 4671NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

ReactomeiR-HSA-74259. Purine catabolism.
SABIO-RKQ96P26.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)
Short name:
cN1B
Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name:
cN-IB
Gene namesi
Name:NT5C1B
Synonyms:AIRP
ORF Names:FKSG85
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:17818. NT5C1B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31800.

Polymorphism and mutation databases

BioMutaiNT5C1B.
DMDMi47116569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Cytosolic 5'-nucleotidase 1BPRO_0000144796Add
BLAST

Proteomic databases

PaxDbiQ96P26.
PeptideAtlasiQ96P26.
PRIDEiQ96P26.
TopDownProteomicsiQ96P26-3. [Q96P26-3]

PTM databases

DEPODiQ96P26.
iPTMnetiQ96P26.
PhosphoSiteiQ96P26.

Expressioni

Tissue specificityi

Highly expressed in testis, placenta and pancreas. Detected at lower levels in heart, kidney, liver and lung.1 Publication

Gene expression databases

BgeeiENSG00000185013.
CleanExiHS_NT5C1B.
ExpressionAtlasiQ96P26. baseline and differential.
GenevisibleiQ96P26. HS.

Organism-specific databases

HPAiHPA056683.

Interactioni

Protein-protein interaction databases

BioGridi124995. 1 interaction.
STRINGi9606.ENSP00000352904.

Structurei

3D structure databases

ProteinModelPortaliQ96P26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi102 – 17675Ser-richAdd
BLAST
Compositional biasi157 – 20145Pro-richAdd
BLAST
Compositional biasi267 – 2715Poly-Gln

Sequence similaritiesi

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGiENOG410IE01. Eukaryota.
ENOG410XPRD. LUCA.
GeneTreeiENSGT00390000017767.
HOVERGENiHBG050426.
KOiK01081.
OMAiPKPKHAI.
OrthoDBiEOG091G0EZE.
PhylomeDBiQ96P26.
TreeFamiTF329831.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96P26-1) [UniParc]FASTAAdd to basket
Also known as: CN-Ib alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS
60 70 80 90 100
LRCCPFQGHS SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR
110 120 130 140 150
GSQESSLRKT DSRGYLVRSQ WSRISRSPST KAPSIDEPRS RNTSAKLPSS
160 170 180 190 200
STSSRTPSTS PSLHDSSPPP LSGQPSLQPP ASPQLPRSLD SRPPTPPEPD
210 220 230 240 250
PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP TEWKSSSQRR
260 270 280 290 300
GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW
310 320 330 340 350
PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG
360 370 380 390 400
PAFRFVKALQ YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY
410 420 430 440 450
GLLIDRFCLT GGKDPIGYLK AYLTNLYIAA DSEKVQEAIQ EGIASATMFD
460 470 480 490 500
GAKDMAYCDT QLRVAFDGDA VLFSDESEHF TKEHGLDKFF QYDTLCESKP
510 520 530 540 550
LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS AASSGARVLK
560 570 580 590 600
TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA
610
AYGFNKKFSS
Length:610
Mass (Da):68,804
Last modified:May 10, 2004 - v2
Checksum:iEEC0BE9E5498B3E9
GO
Isoform 2 (identifier: Q96P26-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-100: Missing.

Note: No experimental confirmation available.
Show »
Length:550
Mass (Da):61,934
Checksum:iC0C913137DFD2479
GO
Isoform 3 (identifier: Q96P26-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.

Note: No experimental confirmation available.
Show »
Length:402
Mass (Da):45,860
Checksum:iBFF1852B61C0EA5B
GO
Isoform 4 (identifier: Q96P26-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     596-610: LGSIAAYGFNKKFSS → KSLGWMS

Note: No experimental confirmation available.
Show »
Length:602
Mass (Da):68,022
Checksum:i1D2A9C840FE9ED0D
GO

Sequence cautioni

The sequence AAK39108 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAK83285 differs from that shown. Reason: Frameshift at position 320. Curated
The sequence BAC04103 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAC44363 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731G → R in AAL11910 (Ref. 2) Curated
Sequence conflicti287 – 2871R → K in AAK39108 (Ref. 1) Curated
Sequence conflicti297 – 2971S → W in AAK39108 (Ref. 1) Curated
Sequence conflicti351 – 3511P → Q in AAL11910 (Ref. 2) Curated
Sequence conflicti362 – 3621V → I in BAC04174 (PubMed:14702039).Curated
Sequence conflicti528 – 5281L → P in CAC44363 (PubMed:11690631).Curated
Sequence conflicti552 – 5521L → F in AAK39108 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 208208Missing in isoform 3. 1 PublicationVSP_010202Add
BLAST
Alternative sequencei41 – 10060Missing in isoform 2. 2 PublicationsVSP_010201Add
BLAST
Alternative sequencei596 – 61015LGSIA…KKFSS → KSLGWMS in isoform 4. 1 PublicationVSP_010203Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356185 mRNA. Translation: AAK39108.1. Different initiation.
AF417165 mRNA. Translation: AAL11910.1.
AK093234 mRNA. Translation: BAC04103.1. Different initiation.
AK093464 mRNA. Translation: BAC04174.1.
AK057444 mRNA. Translation: BAB71489.1.
AC079148 Genomic DNA. Translation: AAX93260.1.
CH471053 Genomic DNA. Translation: EAX00853.1.
BC136387 mRNA. Translation: AAI36388.1.
BC171761 mRNA. Translation: AAI71761.1.
AF352326 mRNA. Translation: AAK83285.1. Frameshift.
AJ295254 mRNA. Translation: CAC44363.1. Different initiation.
CCDSiCCDS33149.1. [Q96P26-2]
CCDS33150.1. [Q96P26-1]
RefSeqiNP_001002006.1. NM_001002006.2. [Q96P26-1]
NP_001186015.1. NM_001199086.1.
NP_001186016.1. NM_001199087.1.
NP_001186017.1. NM_001199088.1.
NP_001186033.1. NM_001199104.1. [Q96P26-4]
NP_150278.2. NM_033253.3. [Q96P26-2]
UniGeneiHs.744358.

Genome annotation databases

EnsembliENST00000304081; ENSP00000305979; ENSG00000185013. [Q96P26-2]
ENST00000359846; ENSP00000352904; ENSG00000185013. [Q96P26-1]
GeneIDi100526794.
93034.
KEGGihsa:100526794.
hsa:93034.
UCSCiuc002rcz.4. human. [Q96P26-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356185 mRNA. Translation: AAK39108.1. Different initiation.
AF417165 mRNA. Translation: AAL11910.1.
AK093234 mRNA. Translation: BAC04103.1. Different initiation.
AK093464 mRNA. Translation: BAC04174.1.
AK057444 mRNA. Translation: BAB71489.1.
AC079148 Genomic DNA. Translation: AAX93260.1.
CH471053 Genomic DNA. Translation: EAX00853.1.
BC136387 mRNA. Translation: AAI36388.1.
BC171761 mRNA. Translation: AAI71761.1.
AF352326 mRNA. Translation: AAK83285.1. Frameshift.
AJ295254 mRNA. Translation: CAC44363.1. Different initiation.
CCDSiCCDS33149.1. [Q96P26-2]
CCDS33150.1. [Q96P26-1]
RefSeqiNP_001002006.1. NM_001002006.2. [Q96P26-1]
NP_001186015.1. NM_001199086.1.
NP_001186016.1. NM_001199087.1.
NP_001186017.1. NM_001199088.1.
NP_001186033.1. NM_001199104.1. [Q96P26-4]
NP_150278.2. NM_033253.3. [Q96P26-2]
UniGeneiHs.744358.

3D structure databases

ProteinModelPortaliQ96P26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124995. 1 interaction.
STRINGi9606.ENSP00000352904.

PTM databases

DEPODiQ96P26.
iPTMnetiQ96P26.
PhosphoSiteiQ96P26.

Polymorphism and mutation databases

BioMutaiNT5C1B.
DMDMi47116569.

Proteomic databases

PaxDbiQ96P26.
PeptideAtlasiQ96P26.
PRIDEiQ96P26.
TopDownProteomicsiQ96P26-3. [Q96P26-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304081; ENSP00000305979; ENSG00000185013. [Q96P26-2]
ENST00000359846; ENSP00000352904; ENSG00000185013. [Q96P26-1]
GeneIDi100526794.
93034.
KEGGihsa:100526794.
hsa:93034.
UCSCiuc002rcz.4. human. [Q96P26-1]

Organism-specific databases

CTDi100526794.
93034.
GeneCardsiNT5C1B.
HGNCiHGNC:17818. NT5C1B.
HPAiHPA056683.
MIMi610526. gene.
neXtProtiNX_Q96P26.
PharmGKBiPA31800.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IE01. Eukaryota.
ENOG410XPRD. LUCA.
GeneTreeiENSGT00390000017767.
HOVERGENiHBG050426.
KOiK01081.
OMAiPKPKHAI.
OrthoDBiEOG091G0EZE.
PhylomeDBiQ96P26.
TreeFamiTF329831.

Enzyme and pathway databases

ReactomeiR-HSA-74259. Purine catabolism.
SABIO-RKQ96P26.

Miscellaneous databases

PROiQ96P26.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185013.
CleanExiHS_NT5C1B.
ExpressionAtlasiQ96P26. baseline and differential.
GenevisibleiQ96P26. HS.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5NT1B_HUMAN
AccessioniPrimary (citable) accession number: Q96P26
Secondary accession number(s): B5MCR0
, B7ZVX7, Q53RX2, Q8N9W3, Q8NA26, Q96DU5, Q96KE6, Q96M25, Q96SA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 7, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.