Q96P16 (RPR1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulation of nuclear pre-mRNA domain-containing protein 1A

Alternative name(s):
Cyclin-dependent kinase inhibitor 2B-related protein
p15INK4B-related protein

Gene names

Name:	RPRD1A
Synonyms:	P15RS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle. Ref.1 Ref.7
Subunit structure	Associates with the RNA polymerase II complex. Ref.7
Induction	Up-regulated in cells overexpressing CDKN2B. Ref.1
Sequence similarities	Belongs to the UPF0400 (RTT103) family. Contains 1 CID domain.
Sequence caution	The sequence AAH10136.1 differs from that shown. Reason: Probable cloning artifact.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation of RNA polymerase II C-terminal domain Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	DNA-directed RNA polymerase II, holoenzyme Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96P16-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96P16-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: MSAFSEAALEKKLSELSNSQQSVQTLSLWLIHHRKHSRPIVTVWERELRKA → MRNSNWRFCQTGIYS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 312	311	Regulation of nuclear pre-mRNA domain-containing protein 1A		PRO_0000311344
Regions
	Domain	2 – 133	132	CID
	Coiled coil	244 – 286	43	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5
Natural variations
	Alternative sequence	1 – 51	51	MSAFS…ELRKA → MRNSNWRFCQTGIYS in isoform 2.		VSP_029531
	Natural variant	21	1	Q → H in a breast cancer sample; somatic mutation. Ref.8		VAR_037229
Experimental info
	Sequence conflict	193	1	E → D in BAA91736. Ref.2
	Sequence conflict	234	1	I → V in BAA91736. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 61785281F31CB328	FASTA	312	35,720
	10 20 30 40 50 60 MSAFSEAALE KKLSELSNSQ QSVQTLSLWL IHHRKHSRPI VTVWERELRK AKPNRKLTFL 70 80 90 100 110 120 YLANDVIQNS KRKGPEFTKD FAPVIVEAFK HVSSETDESC KKHLGRVLSI WEERSVYEND 130 140 150 160 170 180 VLEQLKQALY GDKKPRKRTY EQIKVDENEN CSSLGSPSEP PQTLDLVRAL QDLENAASGD 190 200 210 220 230 240 AAVHQRIASL PVEVQEVSLL DKITDKESGE RLSKMVEDAC MLLADYNGRL AAEIDDRKQL 250 260 270 280 290 300 TRMLADFLRC QKEALAEKEH KLEEYKRKLA RVSLVRKELR SRIQSLPDLS RLPNVTGSHM 310 HLPFAGDIYS ED
	Isoform 2 [UniParc]. Checksum: 09748C553C326E55	FASTA	276	31,625
	10 20 30 40 50 60 MRNSNWRFCQ TGIYSKPNRK LTFLYLANDV IQNSKRKGPE FTKDFAPVIV EAFKHVSSET 70 80 90 100 110 120 DESCKKHLGR VLSIWEERSV YENDVLEQLK QALYGDKKPR KRTYEQIKVD ENENCSSLGS 130 140 150 160 170 180 PSEPPQTLDL VRALQDLENA ASGDAAVHQR IASLPVEVQE VSLLDKITDK ESGERLSKMV 190 200 210 220 230 240 EDACMLLADY NGRLAAEIDD RKQLTRMLAD FLRCQKEALA EKEHKLEEYK RKLARVSLVR 250 260 270 KELRSRIQSL PDLSRLPNVT GSHMHLPFAG DIYSED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of P15RS, a novel P15(INK4b) related gene on G1/S progression." Liu J., Liu H., Zhang X., Gao P., Wang J., Hu Z. Biochem. Biophys. Res. Commun. 299:880-885(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE FUNCTION, INDUCTION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta, Teratocarcinoma and Trachea.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-263 (ISOFORM 1). Tissue: Eye and Muscle.
[5]	Bienvenut W.V., Bilsland A.E., Keith W.N. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12; 107-114; 169-186; 230-237; 243-249 AND 283-291, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Colon carcinoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B." Ni Z., Olsen J.B., Guo X., Zhong G., Ruan E.D., Marcon E., Young P., Guo H., Li J., Moffat J., Emili A., Greenblatt J.F. Transcription 2:237-242(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNA POLYMERASE II COMPLEX, FUNCTION.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF419845 mRNA. Translation: AAL15972.1. AK001518 mRNA. Translation: BAA91736.1. AK292907 mRNA. Translation: BAF85596.1. AK314569 mRNA. Translation: BAG37150.1. CH471088 Genomic DNA. Translation: EAX01365.1. CH471088 Genomic DNA. Translation: EAX01366.1. CH471088 Genomic DNA. Translation: EAX01367.1. BC000225 mRNA. Translation: AAH00225.2. BC010136 mRNA. Translation: AAH10136.1. Sequence problems.
IPI	IPI00062336. IPI00102425. IPI00385260.
RefSeq	NP_060640.2. NM_018170.3.
UniGene	Hs.464912.
3D structure databases
ProteinModelPortal	Q96P16.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96P16. 5 interactions.
STRING	9606.ENSP00000349955.
PTM databases
PhosphoSite	Q96P16.
Polymorphism databases
DMDM	74717048.
Proteomic databases
PaxDb	Q96P16.
PRIDE	Q96P16.
Protocols and materials databases
DNASU	55197.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319040; ENSP00000314602; ENSG00000141425. ENST00000337059; ENSP00000337476; ENSG00000141425. ENST00000357384; ENSP00000349955; ENSG00000141425. ENST00000399022; ENSP00000381984; ENSG00000141425. ENST00000588737; ENSP00000465444; ENSG00000141425. ENST00000589050; ENSP00000468209; ENSG00000141425. ENST00000590898; ENSP00000467991; ENSG00000141425.
GeneID	55197.
KEGG	hsa:55197.
UCSC	uc002kze.1. human. uc002kzg.3. human. uc010dmx.3. human.
Organism-specific databases
CTD	55197.
GeneCards	GC18M033569.
H-InvDB	HIX0014404.
HGNC	HGNC:25560. RPRD1A.
HPA	HPA040602.
MIM	610347. gene.
neXtProt	NX_Q96P16.
PharmGKB	PA162402042.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG291577.
HOGENOM	HOG000007456.
HOVERGEN	HBG051177.
InParanoid	Q96P16.
OMA	SDFLRCQ.
OrthoDB	EOG4F4SBP.
PhylomeDB	Q96P16.
Gene expression databases
Bgee	Q96P16.
CleanEx	HS_RPRD1A.
Genevestigator	Q96P16.
Family and domain databases
Gene3D	1.25.40.90. 1 hit.
InterPro	IPR006569. CID_dom. IPR008942. ENTH_VHS. IPR006903. RNA_pol_II-bd. [Graphical view]
Pfam	PF04818. CTD_bind. 1 hit. [Graphical view]
SMART	SM00582. RPR. 1 hit. [Graphical view]
SUPFAM	SSF48464. ENTH_VHS. 1 hit.
PROSITE	PS51391. CID. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPRD1A. human.
GenomeRNAi	55197.
NextBio	59071.
SOURCE	Search...

Entry information

Entry name

RPR1A_HUMAN

Accession

Primary (citable) accession number: Q96P16
Secondary accession number(s): A8KA42 , B2RBA3, Q7Z5G8, Q96FY9, Q9NVL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.