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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1

Gene

WFIKKN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei122 – 1232Reactive bondPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI17.951.

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1
Alternative name(s):
Growth and differentiation factor-associated serum protein 2
Short name:
GASP-2
Short name:
hGASP-2
WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing protein
Gene namesi
Name:WFIKKN1
Synonyms:C16orf12, GASP2, WFIKKN
ORF Names:PSEC0040
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:30912. WFIKKN1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134897686.

Polymorphism and mutation databases

BioMutaiWFIKKN1.
DMDMi74717044.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 548529WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1PRO_0000307816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 66By similarity
Disulfide bondi49 ↔ 70By similarity
Disulfide bondi53 ↔ 65By similarity
Disulfide bondi59 ↔ 75By similarity
Disulfide bondi116 ↔ 146By similarity
Disulfide bondi120 ↔ 139By similarity
Disulfide bondi128 ↔ 157By similarity
Disulfide bondi207 ↔ 263By similarity
Disulfide bondi299 ↔ 351By similarity
Disulfide bondi306 ↔ 334By similarity
Disulfide bondi326 ↔ 347By similarity
Disulfide bondi359 ↔ 409By similarity
Disulfide bondi368 ↔ 392By similarity
Disulfide bondi384 ↔ 405By similarity
Disulfide bondi417 ↔ 489By similarity
Disulfide bondi420 ↔ 491By similarity
Disulfide bondi431 ↔ 540By similarity
Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ96NZ8.
PaxDbiQ96NZ8.
PeptideAtlasiQ96NZ8.
PRIDEiQ96NZ8.
TopDownProteomicsiQ96NZ8.

PTM databases

iPTMnetiQ96NZ8.
PhosphoSiteiQ96NZ8.

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, liver, placenta, and lung.

Gene expression databases

BgeeiQ96NZ8.
CleanExiHS_WFIKKN1.
GenevisibleiQ96NZ8. HS.

Organism-specific databases

HPAiCAB025858.
HPA043362.
HPA044237.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MSTNO147934EBI-2363713,EBI-8542977

Protein-protein interaction databases

BioGridi125565. 1 interaction.
IntActiQ96NZ8. 9 interactions.
STRINGi9606.ENSP00000324763.

Structurei

Secondary structure

1
548
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi358 – 3603Combined sources
Beta strandi372 – 3787Combined sources
Helixi379 – 3813Combined sources
Beta strandi383 – 3897Combined sources
Beta strandi391 – 3933Combined sources
Beta strandi399 – 4013Combined sources
Helixi402 – 4087Combined sources
Beta strandi409 – 4113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DDINMR-A357-411[»]
2DDJNMR-A357-411[»]
ProteinModelPortaliQ96NZ8.
SMRiQ96NZ8. Positions 110-156, 166-287, 297-411.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96NZ8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 7954WAPPROSITE-ProRule annotationAdd
BLAST
Domaini108 – 15952Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini186 – 27994Ig-like C2-typeAdd
BLAST
Domaini299 – 35153BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd
BLAST
Domaini359 – 40951BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd
BLAST
Domaini409 – 540132NTRPROSITE-ProRule annotationAdd
BLAST

Domaini

The second BPTI/Kunitz inhibitor domain is able to inhibit trypsin. It has however no activity toward chymotrypsin, elastase, plasmin, pancreatic kallikrein, lung tryptase, plasma kallikrein, thrombin, urokinase or tissue plasminogen activator.1 Publication

Sequence similaritiesi

Belongs to the WFIKKN family.Curated
Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation
Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4597. Eukaryota.
ENOG41102GD. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000154855.
HOVERGENiHBG108676.
InParanoidiQ96NZ8.
OMAiQRENLIM.
OrthoDBiEOG754HSR.
PhylomeDBiQ96NZ8.
TreeFamiTF315349.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR002223. Kunitz_BPTI.
IPR001134. Netrin_domain.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96NZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPALRPLLPL LLLLRLTSGA GLLPGLGSHP GVCPNQLSPN LWVDAQSTCE
60 70 80 90 100
RECSRDQDCA AAEKCCINVC GLHSCVAARF PGSPAAPTTA ASCEGFVCPQ
110 120 130 140 150
QGSDCDIWDG QPVCRCRDRC EKEPSFTCAS DGLTYYNRCY MDAEACLRGL
160 170 180 190 200
HLHIVPCKHV LSWPPSSPGP PETTARPTPG AAPVPPALYS SPSPQAVQVG
210 220 230 240 250
GTASLHCDVS GRPPPAVTWE KQSHQRENLI MRPDQMYGNV VVTSIGQLVL
260 270 280 290 300
YNARPEDAGL YTCTARNAAG LLRADFPLSV VQREPARDAA PSIPAPAECL
310 320 330 340 350
PDVQACTGPT SPHLVLWHYD PQRGGCMTFP ARGCDGAARG FETYEACQQA
360 370 380 390 400
CARGPGDACV LPAVQGPCRG WEPRWAYSPL LQQCHPFVYG GCEGNGNNFH
410 420 430 440 450
SRESCEDACP VPRTPPCRAC RLRSKLALSL CRSDFAIVGR LTEVLEEPEA
460 470 480 490 500
AGGIARVALE DVLKDDKMGL KFLGTKYLEV TLSGMDWACP CPNMTAGDGP
510 520 530 540
LVIMGEVRDG VAVLDAGSYV RAASEKRVKK ILELLEKQAC ELLNRFQD
Length:548
Mass (Da):58,798
Last modified:December 1, 2001 - v1
Checksum:i72BB28708D3EFB1F
GO

Sequence cautioni

The sequence AAK61237.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAC11566.1 differs from that shown. Reason: Frameshift at position 147. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF422194 mRNA. Translation: AAL18839.1.
AK075356 mRNA. Translation: BAC11566.1. Frameshift.
AE006464 Genomic DNA. Translation: AAK61237.1. Sequence problems.
BC101602 mRNA. Translation: AAI01603.1.
BC101606 mRNA. Translation: AAI01607.1.
CCDSiCCDS10414.1.
RefSeqiNP_444514.1. NM_053284.2.
UniGeneiHs.345818.

Genome annotation databases

EnsembliENST00000319070; ENSP00000324763; ENSG00000127578.
GeneIDi117166.
KEGGihsa:117166.
UCSCiuc002cht.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF422194 mRNA. Translation: AAL18839.1.
AK075356 mRNA. Translation: BAC11566.1. Frameshift.
AE006464 Genomic DNA. Translation: AAK61237.1. Sequence problems.
BC101602 mRNA. Translation: AAI01603.1.
BC101606 mRNA. Translation: AAI01607.1.
CCDSiCCDS10414.1.
RefSeqiNP_444514.1. NM_053284.2.
UniGeneiHs.345818.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DDINMR-A357-411[»]
2DDJNMR-A357-411[»]
ProteinModelPortaliQ96NZ8.
SMRiQ96NZ8. Positions 110-156, 166-287, 297-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125565. 1 interaction.
IntActiQ96NZ8. 9 interactions.
STRINGi9606.ENSP00000324763.

Protein family/group databases

MEROPSiI17.951.

PTM databases

iPTMnetiQ96NZ8.
PhosphoSiteiQ96NZ8.

Polymorphism and mutation databases

BioMutaiWFIKKN1.
DMDMi74717044.

Proteomic databases

EPDiQ96NZ8.
PaxDbiQ96NZ8.
PeptideAtlasiQ96NZ8.
PRIDEiQ96NZ8.
TopDownProteomicsiQ96NZ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319070; ENSP00000324763; ENSG00000127578.
GeneIDi117166.
KEGGihsa:117166.
UCSCiuc002cht.2. human.

Organism-specific databases

CTDi117166.
GeneCardsiWFIKKN1.
HGNCiHGNC:30912. WFIKKN1.
HPAiCAB025858.
HPA043362.
HPA044237.
MIMi608021. gene.
neXtProtiNX_Q96NZ8.
PharmGKBiPA134897686.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4597. Eukaryota.
ENOG41102GD. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000154855.
HOVERGENiHBG108676.
InParanoidiQ96NZ8.
OMAiQRENLIM.
OrthoDBiEOG754HSR.
PhylomeDBiQ96NZ8.
TreeFamiTF315349.

Miscellaneous databases

EvolutionaryTraceiQ96NZ8.
GeneWikiiWFIKKN1.
GenomeRNAii117166.
PROiQ96NZ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ96NZ8.
CleanExiHS_WFIKKN1.
GenevisibleiQ96NZ8. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR002223. Kunitz_BPTI.
IPR001134. Netrin_domain.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human protein containing multiple types of protease-inhibitory modules."
    Trexler M., Banyai L., Patthy L.
    Proc. Natl. Acad. Sci. U.S.A. 98:3705-3709(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  3. "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
    Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.
    Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains."
    Hill J.J., Qiu Y., Hewick R.M., Wolfman N.M.
    Mol. Endocrinol. 17:1144-1154(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein."
    Nagy A., Trexler M., Patthy L.
    Eur. J. Biochem. 270:2101-2107(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  7. "Second Kunitz-type protease inhibitor domain of the human WFIKKN1 protein."
    Liepinsh E., Nagy A., Trexler M., Patthy L., Otting G.
    J. Biomol. NMR 35:73-78(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 357-411.

Entry informationi

Entry nameiWFKN1_HUMAN
AccessioniPrimary (citable) accession number: Q96NZ8
Secondary accession number(s): Q7LDW0, Q8NBQ1, Q96S20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.