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Protein

Calcium/calmodulin-dependent protein kinase type 1G

Gene

CAMK1G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin. Binding of calmodulin is thought to result in a conformational change and leads to activation through phosphorylation by CAMKK1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATPPROSITE-ProRule annotation1
Active sitei143Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 37ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00231-MONOMER.
BRENDAi2.7.11.17. 2681.
SignaLinkiQ96NX5.
SIGNORiQ96NX5.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type 1G (EC:2.7.11.17)
Alternative name(s):
CaM kinase I gamma
Short name:
CaM kinase IG
Short name:
CaM-KI gamma
Short name:
CaMKI gamma
Short name:
CaMKIG
CaMK-like CREB kinase III
Short name:
CLICK III
Gene namesi
Name:CAMK1G
Synonyms:CLICK3, VWS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14585. CAMK1G.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57172.
OpenTargetsiENSG00000008118.
PharmGKBiPA26049.

Chemistry databases

ChEMBLiCHEMBL5258.
GuidetoPHARMACOLOGYi1954.

Polymorphism and mutation databases

BioMutaiCAMK1G.
DMDMi73620970.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860841 – 476Calcium/calmodulin-dependent protein kinase type 1GAdd BLAST476

Post-translational modificationi

May be prenylated on Cys-473.By similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiQ96NX5.
PeptideAtlasiQ96NX5.
PRIDEiQ96NX5.

PTM databases

iPTMnetiQ96NX5.
PhosphoSitePlusiQ96NX5.

Expressioni

Tissue specificityi

Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.1 Publication

Gene expression databases

BgeeiENSG00000008118.
CleanExiHS_CAMK1G.
ExpressionAtlasiQ96NX5. baseline and differential.
GenevisibleiQ96NX5. HS.

Organism-specific databases

HPAiCAB069890.

Interactioni

Protein-protein interaction databases

BioGridi121424. 6 interactors.
IntActiQ96NX5. 2 interactors.
STRINGi9606.ENSP00000009105.

Chemistry databases

BindingDBiQ96NX5.

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 22Combined sources4
Beta strandi23 – 31Combined sources9
Beta strandi33 – 42Combined sources10
Turni43 – 45Combined sources3
Beta strandi48 – 55Combined sources8
Helixi64 – 74Combined sources11
Beta strandi83 – 88Combined sources6
Beta strandi90 – 97Combined sources8
Helixi105 – 112Combined sources8
Helixi117 – 136Combined sources20
Helixi146 – 148Combined sources3
Beta strandi150 – 155Combined sources6
Beta strandi160 – 162Combined sources3
Helixi177 – 180Combined sources4
Turni188 – 190Combined sources3
Beta strandi191 – 193Combined sources3
Helixi198 – 214Combined sources17
Turni218 – 221Combined sources4
Helixi224 – 233Combined sources10
Turni240 – 245Combined sources6
Helixi248 – 258Combined sources11
Turni262 – 264Combined sources3
Helixi268 – 272Combined sources5
Helixi275 – 278Combined sources4
Helixi288 – 298Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JAMX-ray1.70A/B18-316[»]
ProteinModelPortaliQ96NX5.
SMRiQ96NX5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96NX5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST255

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 317Autoinhibitory domainBy similarityAdd BLAST41
Regioni297 – 318Calmodulin-bindingBy similarityAdd BLAST22

Domaini

The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ96NX5.
KOiK08794.
OMAiVGPCGCC.
OrthoDBiEOG091G0HK9.
PhylomeDBiQ96NX5.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96NX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRKEEDDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRLTGKLFA
60 70 80 90 100
LKCIKKSPAF RDSSLENEIA VLKKIKHENI VTLEDIYEST THYYLVMQLV
110 120 130 140 150
SGGELFDRIL ERGVYTEKDA SLVIQQVLSA VKYLHENGIV HRDLKPENLL
160 170 180 190 200
YLTPEENSKI MITDFGLSKM EQNGIMSTAC GTPGYVAPEV LAQKPYSKAV
210 220 230 240 250
DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES PFWDDISESA
260 270 280 290 300
KDFICHLLEK DPNERYTCEK ALSHPWIDGN TALHRDIYPS VSLQIQKNFA
310 320 330 340 350
KSKWRQAFNA AAVVHHMRKL HMNLHSPGVR PEVENRPPET QASETSRPSS
360 370 380 390 400
PEITITEAPV LDHSVALPAL TQLPCQHGRR PTAPGGRSLN CLVNGSLHIS
410 420 430 440 450
SSLVPMHQGS LAAGPCGCCS SCLNIGSKGK SSYCSEPTLL KKANKKQNFK
460 470
SEVMVPVKAS GSSHCRAGQT GVCLIM
Length:476
Mass (Da):53,087
Last modified:August 16, 2005 - v3
Checksum:iDD009D9CF2FE3CBE
GO
Isoform 2 (identifier: Q96NX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     448-476: NFKSEVMVPVKASGSSHCRAGQTGVCLIM → YVFLAKDGAPAWV

Show »
Length:460
Mass (Da):51,486
Checksum:iA16971DC50344496
GO

Sequence cautioni

The sequence CAB41259 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti427S → N in AAL28100 (PubMed:10645953).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040600259E → Q.1 PublicationCorresponds to variant rs35561962dbSNPEnsembl.1
Natural variantiVAR_020530329V → I.2 PublicationsCorresponds to variant rs11119315dbSNPEnsembl.1
Natural variantiVAR_040601443A → T in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012138448 – 476NFKSE…VCLIM → YVFLAKDGAPAWV in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF428261 mRNA. Translation: AAL28100.1.
AY212935 mRNA. Translation: AAP29964.1.
AL049688 mRNA. Translation: CAB41259.1. Different initiation.
AL023754 Genomic DNA. Translation: CAI19991.1.
BC032787 mRNA. Translation: AAH32787.1.
CCDSiCCDS1486.1. [Q96NX5-1]
RefSeqiNP_065172.1. NM_020439.2. [Q96NX5-1]
XP_016857355.1. XM_017001866.1. [Q96NX5-1]
UniGeneiHs.199068.

Genome annotation databases

EnsembliENST00000009105; ENSP00000009105; ENSG00000008118. [Q96NX5-1]
ENST00000361322; ENSP00000354861; ENSG00000008118. [Q96NX5-1]
GeneIDi57172.
KEGGihsa:57172.
UCSCiuc001hhd.4. human. [Q96NX5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF428261 mRNA. Translation: AAL28100.1.
AY212935 mRNA. Translation: AAP29964.1.
AL049688 mRNA. Translation: CAB41259.1. Different initiation.
AL023754 Genomic DNA. Translation: CAI19991.1.
BC032787 mRNA. Translation: AAH32787.1.
CCDSiCCDS1486.1. [Q96NX5-1]
RefSeqiNP_065172.1. NM_020439.2. [Q96NX5-1]
XP_016857355.1. XM_017001866.1. [Q96NX5-1]
UniGeneiHs.199068.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JAMX-ray1.70A/B18-316[»]
ProteinModelPortaliQ96NX5.
SMRiQ96NX5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121424. 6 interactors.
IntActiQ96NX5. 2 interactors.
STRINGi9606.ENSP00000009105.

Chemistry databases

BindingDBiQ96NX5.
ChEMBLiCHEMBL5258.
GuidetoPHARMACOLOGYi1954.

PTM databases

iPTMnetiQ96NX5.
PhosphoSitePlusiQ96NX5.

Polymorphism and mutation databases

BioMutaiCAMK1G.
DMDMi73620970.

Proteomic databases

PaxDbiQ96NX5.
PeptideAtlasiQ96NX5.
PRIDEiQ96NX5.

Protocols and materials databases

DNASUi57172.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000009105; ENSP00000009105; ENSG00000008118. [Q96NX5-1]
ENST00000361322; ENSP00000354861; ENSG00000008118. [Q96NX5-1]
GeneIDi57172.
KEGGihsa:57172.
UCSCiuc001hhd.4. human. [Q96NX5-1]

Organism-specific databases

CTDi57172.
DisGeNETi57172.
GeneCardsiCAMK1G.
HGNCiHGNC:14585. CAMK1G.
HPAiCAB069890.
MIMi614994. gene.
neXtProtiNX_Q96NX5.
OpenTargetsiENSG00000008118.
PharmGKBiPA26049.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ96NX5.
KOiK08794.
OMAiVGPCGCC.
OrthoDBiEOG091G0HK9.
PhylomeDBiQ96NX5.
TreeFamiTF314166.

Enzyme and pathway databases

BioCyciZFISH:HS00231-MONOMER.
BRENDAi2.7.11.17. 2681.
SignaLinkiQ96NX5.
SIGNORiQ96NX5.

Miscellaneous databases

EvolutionaryTraceiQ96NX5.
GenomeRNAii57172.
PROiQ96NX5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000008118.
CleanExiHS_CAMK1G.
ExpressionAtlasiQ96NX5. baseline and differential.
GenevisibleiQ96NX5. HS.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCC1G_HUMAN
AccessioniPrimary (citable) accession number: Q96NX5
Secondary accession number(s): Q86UH5, Q9Y3J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: August 16, 2005
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.