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Reviewed, UniProtKB/Swiss-Prot Q96NN9 (AIFM3_HUMAN)

Last modified November 25, 2008. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Apoptosis-inducing factor 3
    EC=1.-.-.-
Alternative name(s):
    Apoptosis-inducing factor-like protein
Gene names
Name: AIFM3
Synonyms: AIFL
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.

Subcellular location

Mitochondrion. Note= Does not translocate to the nucleus upon induction of apoptosis.

Tissue specificity

Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).

Domain

The Rieske domain induces apoptosis.

Sequence similarities

Belongs to the FAD-dependent oxidoreductase family.

Contains 1 Rieske domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96NN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96NN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: P → PGAALPT
     587-593: Missing.
Isoform 3 (identifier: Q96NN9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     587-593: Missing.
Notes: No experimental confirmation available.
Isoform 4 (identifier: Q96NN9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     2-383: Missing.
     384-427: FENNRVKFYM...VRADVCVVGI → MKASGRQPLE...GEWTQASVLS
     552-605: Missing.
Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Apoptosis-inducing factor 3
PRO_0000255660

Regions

Domain70 – 16596Rieske
Nucleotide binding201 – 2055FAD Potential

Sites

Metal binding1091Iron-sulfur (2Fe-2S) By similarity
Metal binding1111Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding1281Iron-sulfur (2Fe-2S) By similarity
Metal binding1311Iron-sulfur (2Fe-2S); via pros nitrogen
Binding site2351FAD Potential
Binding site2401FAD Potential
Binding site2701FAD; via amide nitrogen and carbonyl oxygen Potential
Binding site4671FAD Potential
Binding site5141FAD; via carbonyl oxygen Potential

Natural variations

Alternative sequence2 – 383382Missing in isoform 4.
VSP_021299
Alternative sequence101P → PGAALPT in isoform 2.
VSP_021300
Alternative sequence384 – 42744FENNR…CVVGI → MKASGRQPLEDQSGAGTSSA CCRVWRVTTQKGGTGEWTQA SVLS in isoform 4.
VSP_021301
Alternative sequence552 – 60554Missing in isoform 4.
VSP_021302
Alternative sequence587 – 5937Missing in isoform 2 and isoform 3.
VSP_021303

Experimental info

Sequence conflict1651V → A in BAC04434. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: C1DF20AE2AF2E262

FASTA60566,791
        10         20         30         40         50         60 
MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRAY QGNGTARHFH TEERLSTPHP 

        70         80         90        100        110        120 
YPSPQDCVEA AVCHVKDLEN GQMREVELGW GKVLLVKDNG EFHALGHKCP HYGAPLVKGV 

       130        140        150        160        170        180 
LSRGRVRCPW HGACFNISTG DLEDFPGLDS LHKFQVKIEK EKVYVRASKQ ALQLQRRTKV 

       190        200        210        220        230        240 
MAKCISPSAG YSSSTNVLIV GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRPKLSK 

       250        260        270        280        290        300 
SLDTQPEQLA LRPKEFFRAY GIEVLTEAQV VTVDVRTKKV VFKDGFKLEY SKLLLAPGSS 

       310        320        330        340        350        360 
PKTLSCKGKE VENVFTIRTP EDANRVVRLA RGRNVVVVGA GFLGMEVAAY LTEKAHSVSV 

       370        380        390        400        410        420 
VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL RGQEGKLKEV VLKSSKVVRA 

       430        440        450        460        470        480 
DVCVVGIGAV PATGFLRQSG IGLDSRGFIP VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK 

       490        500        510        520        530        540 
VNIPHWQMAH AQGRVAAQNM LAQEAEMSTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL 

       550        560        570        580        590        600 
EELKFVAFYT KGDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFVL HSKTGDMSWL 


TGKGS 

« Hide

Isoform 2 [UniParc].

Checksum: AEF75FBE911988A6
Show »

60466,477
Isoform 3 [UniParc].

Checksum: E49D7ED5BBF7F30E
Show »

59865,966
Isoform 4 [UniParc].

Checksum: 148096FF15E7C7B3
Show »

16918,384

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a human AIF-like gene with ability to induce apoptosis."
Xie Q., Lin T., Zhang Y., Zheng J., Bonanno J.A.
J. Biol. Chem. 280:19673-19681(2005) [PubMed: 15764604] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4).
Tissue: Brain and Caudate nucleus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Pancreas.

Cross-references

Sequence databases

CR456342 mRNA. Translation: CAG30228.1.
AK055035 mRNA. Translation: BAB70841.1.
AK094844 mRNA. Translation: BAC04434.1.
AK126917 mRNA. Translation: BAC86750.1.
BC032485 mRNA. Translation: AAH32485.1.
RefSeqNP_001018070.1.
NP_653305.1.
UniGeneHs.163543

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSG00000183773. Homo sapiens. [Contig view]
GeneID150209.
KEGGhsa:150209.

Organism-specific databases

HGNCHGNC:26398. AIFM3.
HPAHPA001271.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOVERGENQ96NN9.

Gene expression databases

ArrayExpressQ96NN9.
CleanExHS_AIFM3.
GermOnlineENSG00000183773. Homo sapiens.

Family and domain databases

InterProIPR000759. Adrndx_reductase.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR001327. Pyr_OxRdtase_NAD_bd.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR005805. Rieske_C.
IPR005806. Rieske_reg.
[Graphical view]
Gene3DG3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PfamPF00070. Pyr_redox. 2 hits.
PF07992. Pyr_redox_2. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSPR00419. ADXRDTASE.
PR00368. FADPNR.
PR00469. PNDRDTASEII.
ProDomPD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio86362.

Entry information

Entry nameAIFM3_HUMAN
AccessionPrimary (citable) accession number: Q96NN9
Secondary accession number(s): Q6ZT44, Q8N1V3, Q8N5E0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents