ID   SCLT1_HUMAN             Reviewed;         688 AA.
AC   Q96NL6; A4QN04; Q0VAH2; Q6P2M4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 2.
DT   24-JAN-2024, entry version 148.
DE   RecName: Full=Sodium channel and clathrin linker 1;
DE   AltName: Full=Sodium channel-associated protein 1;
GN   Name=SCLT1; Synonyms=SAP1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT CYS-441.
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT CYS-441.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=23348840; DOI=10.1101/gad.207043.112;
RA   Tanos B.E., Yang H.J., Soni R., Wang W.J., Macaluso F.P., Asara J.M.,
RA   Tsou M.F.;
RT   "Centriole distal appendages promote membrane docking, leading to cilia
RT   initiation.";
RL   Genes Dev. 27:163-168(2013).
CC   -!- FUNCTION: Adapter protein that links SCN10A to clathrin. Regulates
CC       SCN10A channel activity, possibly by promoting channel internalization
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with SCN10A and clathrin. Identified in a complex
CC       containing SCN10A, clathrin and SCLT1 (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q96NL6-3; P42858: HTT; NbExp=3; IntAct=EBI-25961722, EBI-466029;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome, centriole {ECO:0000269|PubMed:23348840}.
CC       Note=Localizes to the distal appendage region of the centriole, which
CC       anchors the mother centriole to the plasma membrane.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=Q96NL6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96NL6-2; Sequence=VSP_030907;
CC       Name=3;
CC         IsoId=Q96NL6-3; Sequence=VSP_030906;
CC       Name=4;
CC         IsoId=Q96NL6-4; Sequence=VSP_030904, VSP_030905;
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DR   EMBL; AK055217; BAB70876.1; -; mRNA.
DR   EMBL; AC093783; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC093826; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471056; EAX05167.1; -; Genomic_DNA.
DR   EMBL; BC064428; AAH64428.1; -; mRNA.
DR   EMBL; BC121057; AAI21058.1; -; mRNA.
DR   EMBL; BC121058; AAI21059.1; -; mRNA.
DR   EMBL; BC128051; AAI28052.1; -; mRNA.
DR   CCDS; CCDS3740.1; -. [Q96NL6-1]
DR   CCDS; CCDS77958.1; -. [Q96NL6-4]
DR   RefSeq; NP_001287826.1; NM_001300897.1. [Q96NL6-4]
DR   RefSeq; NP_001287827.1; NM_001300898.1.
DR   RefSeq; NP_653244.2; NM_144643.3. [Q96NL6-1]
DR   AlphaFoldDB; Q96NL6; -.
DR   SMR; Q96NL6; -.
DR   BioGRID; 126318; 173.
DR   IntAct; Q96NL6; 165.
DR   MINT; Q96NL6; -.
DR   STRING; 9606.ENSP00000281142; -.
DR   GlyGen; Q96NL6; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q96NL6; -.
DR   PhosphoSitePlus; Q96NL6; -.
DR   BioMuta; SCLT1; -.
DR   DMDM; 300669665; -.
DR   EPD; Q96NL6; -.
DR   jPOST; Q96NL6; -.
DR   MassIVE; Q96NL6; -.
DR   MaxQB; Q96NL6; -.
DR   PaxDb; 9606-ENSP00000281142; -.
DR   PeptideAtlas; Q96NL6; -.
DR   ProteomicsDB; 77532; -. [Q96NL6-1]
DR   ProteomicsDB; 77533; -. [Q96NL6-2]
DR   ProteomicsDB; 77534; -. [Q96NL6-3]
DR   ProteomicsDB; 77535; -. [Q96NL6-4]
DR   Pumba; Q96NL6; -.
DR   Antibodypedia; 45370; 189 antibodies from 22 providers.
DR   DNASU; 132320; -.
DR   Ensembl; ENST00000281142.10; ENSP00000281142.5; ENSG00000151466.12. [Q96NL6-1]
DR   Ensembl; ENST00000439369.6; ENSP00000395292.2; ENSG00000151466.12. [Q96NL6-3]
DR   Ensembl; ENST00000511426.5; ENSP00000420861.1; ENSG00000151466.12. [Q96NL6-4]
DR   GeneID; 132320; -.
DR   KEGG; hsa:132320; -.
DR   MANE-Select; ENST00000281142.10; ENSP00000281142.5; NM_144643.4; NP_653244.2.
DR   UCSC; uc003igp.3; human. [Q96NL6-1]
DR   AGR; HGNC:26406; -.
DR   CTD; 132320; -.
DR   DisGeNET; 132320; -.
DR   GeneCards; SCLT1; -.
DR   HGNC; HGNC:26406; SCLT1.
DR   HPA; ENSG00000151466; Low tissue specificity.
DR   MalaCards; SCLT1; -.
DR   MIM; 611399; gene.
DR   neXtProt; NX_Q96NL6; -.
DR   OpenTargets; ENSG00000151466; -.
DR   Orphanet; 110; Bardet-Biedl syndrome.
DR   PharmGKB; PA147357369; -.
DR   VEuPathDB; HostDB:ENSG00000151466; -.
DR   eggNOG; ENOG502QS6B; Eukaryota.
DR   GeneTree; ENSGT00730000111168; -.
DR   HOGENOM; CLU_025503_0_0_1; -.
DR   InParanoid; Q96NL6; -.
DR   OMA; ESPAPWI; -.
DR   OrthoDB; 2951938at2759; -.
DR   PhylomeDB; Q96NL6; -.
DR   TreeFam; TF331372; -.
DR   PathwayCommons; Q96NL6; -.
DR   Reactome; R-HSA-5620912; Anchoring of the basal body to the plasma membrane.
DR   SignaLink; Q96NL6; -.
DR   BioGRID-ORCS; 132320; 12 hits in 1143 CRISPR screens.
DR   ChiTaRS; SCLT1; human.
DR   GenomeRNAi; 132320; -.
DR   Pharos; Q96NL6; Tbio.
DR   PRO; PR:Q96NL6; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; Q96NL6; Protein.
DR   Bgee; ENSG00000151466; Expressed in buccal mucosa cell and 142 other cell types or tissues.
DR   ExpressionAtlas; Q96NL6; baseline and differential.
DR   GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR   GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR   GO; GO:0097539; C:ciliary transition fiber; IDA:MGI.
DR   GO; GO:0071439; C:clathrin complex; IEA:Ensembl.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0030276; F:clathrin binding; IEA:Ensembl.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:Ensembl.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:0045162; P:clustering of voltage-gated sodium channels; IEA:Ensembl.
DR   InterPro; IPR038911; SCLT1.
DR   PANTHER; PTHR35970; SODIUM CHANNEL AND CLATHRIN LINKER 1; 1.
DR   PANTHER; PTHR35970:SF1; SODIUM CHANNEL AND CLATHRIN LINKER 1; 1.
DR   Genevisible; Q96NL6; HS.
PE   1: Evidence at protein level;
KW   Acetylation; Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Phosphoprotein; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0007744|PubMed:22814378"
FT   CHAIN           2..688
FT                   /note="Sodium channel and clathrin linker 1"
FT                   /id="PRO_0000317127"
FT   COILED          69..673
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0007744|PubMed:22814378"
FT   MOD_RES         681
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:G5E861"
FT   VAR_SEQ         79
FT                   /note="K -> A (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030904"
FT   VAR_SEQ         80..688
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030905"
FT   VAR_SEQ         98..610
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030906"
FT   VAR_SEQ         230..610
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030907"
FT   VARIANT         441
FT                   /note="S -> C (in dbSNP:rs10028124)"
FT                   /evidence="ECO:0000269|PubMed:14702039, ECO:0000269|Ref.3"
FT                   /id="VAR_038481"
SQ   SEQUENCE   688 AA;  80910 MW;  9432F258529C47D3 CRC64;
     MAAEIDFLRE QNRRLNEDFR RYQMESFSKY SSVQKAVCQG EGDDTFENLV FDQSFLAPLV
     TEYDKHLGEL NGQLKYYQKQ VGEMKLQLEN VIKENERLHS ELKDAVEKKL EAFPLGTEVG
     TDIYADDETV RNLQEQLQLA NQEKTQAVEL WQTVSQELDR LHKLYQEHMT EAQIHVFESQ
     KQKDQLFDFQ QLTKQLHVTN ENMEVTNQQF LKTVTEQSVI IEQLRKKLRQ AKLELRVAVA
     KVEELTNVTE DLQGQMKKKE KDVVSAHGRE EASDRRLQQL QSSIKQLEIR LCVTIQEANQ
     LRTENTHLEK QTRELQAKCN ELENERYEAI VRARNSMQLL EEANLQKSQA LLEEKQKEED
     IEKMKETVSR FVQDATIRTK KEVANTKKQC NIQISRLTEE LSALQMECAE KQGQIERVIK
     EKKAVEEELE KIYREGRGNE SDYRKLEEMH QRFLVSERSK DDLQLRLTRA ENRIKQLETD
     SSEEISRYQE MIQKLQNVLE SERENCGLVS EQRLKLQQEN KQLRKETESL RKIALEAQKK
     AKVKISTMEH EFSIKERGFE VQLREMEDSN RNSIVELRHL LATQQKAANR WKEETKKLTE
     SAEIRINNLK SELSRQKLHT QELLSQLEMA NEKVAENEKL ILEHQEKANR LQRRLSQAEE
     RAASASQQLS VITVQRRKAA SLMNLENI
//