ID   TMM74_HUMAN             Reviewed;         305 AA.
AC   Q96NL1;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   24-JAN-2024, entry version 130.
DE   RecName: Full=Transmembrane protein 74;
GN   Name=TMEM74;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=18294959; DOI=10.1016/j.bbrc.2008.02.055;
RA   Yu C., Wang L., Lv B., Lu Y., Zeng L., Chen Y., Ma D., Shi T., Wang L.;
RT   "TMEM74, a lysosome and autophagosome protein, regulates autophagy.";
RL   Biochem. Biophys. Res. Commun. 369:622-629(2008).
RN   [4]
RP   FUNCTION.
RX   PubMed=19029833; DOI=10.4161/auto.5.1.7247;
RA   He P., Peng Z., Luo Y., Wang L., Yu P., Deng W., An Y., Shi T., Ma D.;
RT   "High-throughput functional screening for autophagy-related genes and
RT   identification of TM9SF1 as an autophagosome-inducing gene.";
RL   Autophagy 5:52-60(2009).
CC   -!- FUNCTION: Plays an essential role in autophagy. TMEM74-induced
CC       autophagy may involve PI3K signal transduction.
CC       {ECO:0000269|PubMed:18294959, ECO:0000269|PubMed:19029833}.
CC   -!- INTERACTION:
CC       Q96NL1; Q13520: AQP6; NbExp=3; IntAct=EBI-10292091, EBI-13059134;
CC       Q96NL1; Q969F0: FATE1; NbExp=3; IntAct=EBI-10292091, EBI-743099;
CC       Q96NL1; Q9H400: LIME1; NbExp=3; IntAct=EBI-10292091, EBI-2830566;
CC       Q96NL1; Q5J8X5: MS4A13; NbExp=3; IntAct=EBI-10292091, EBI-12070086;
CC       Q96NL1; Q69YG0: TMEM42; NbExp=3; IntAct=EBI-10292091, EBI-12038591;
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:18294959};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:18294959}. Cytoplasmic
CC       vesicle, autophagosome membrane {ECO:0000269|PubMed:18294959}; Multi-
CC       pass membrane protein {ECO:0000269|PubMed:18294959}.
CC   -!- TISSUE SPECIFICITY: Expressed in heart, lung, and placenta.
CC       {ECO:0000269|PubMed:18294959}.
CC   -!- SIMILARITY: Belongs to the TMEM74 family. {ECO:0000305}.
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DR   EMBL; AK055230; BAB70881.1; -; mRNA.
DR   EMBL; BC030710; AAH30710.1; -; mRNA.
DR   CCDS; CCDS6310.1; -.
DR   RefSeq; NP_694560.1; NM_153015.2.
DR   AlphaFoldDB; Q96NL1; -.
DR   BioGRID; 127621; 124.
DR   IntAct; Q96NL1; 5.
DR   STRING; 9606.ENSP00000297459; -.
DR   TCDB; 8.A.122.2.1; the vesicular membrane neurensin/tmem74 (neurensin/tmem74) family.
DR   PhosphoSitePlus; Q96NL1; -.
DR   BioMuta; TMEM74; -.
DR   DMDM; 74732587; -.
DR   MassIVE; Q96NL1; -.
DR   PaxDb; 9606-ENSP00000297459; -.
DR   PeptideAtlas; Q96NL1; -.
DR   ProteomicsDB; 77529; -.
DR   Antibodypedia; 3040; 105 antibodies from 22 providers.
DR   DNASU; 157753; -.
DR   Ensembl; ENST00000297459.4; ENSP00000297459.3; ENSG00000164841.5.
DR   GeneID; 157753; -.
DR   KEGG; hsa:157753; -.
DR   MANE-Select; ENST00000297459.4; ENSP00000297459.3; NM_153015.3; NP_694560.1.
DR   UCSC; uc003ymy.2; human.
DR   AGR; HGNC:26409; -.
DR   CTD; 157753; -.
DR   DisGeNET; 157753; -.
DR   GeneCards; TMEM74; -.
DR   HGNC; HGNC:26409; TMEM74.
DR   HPA; ENSG00000164841; Tissue enhanced (brain, retina).
DR   MIM; 613935; gene.
DR   neXtProt; NX_Q96NL1; -.
DR   OpenTargets; ENSG00000164841; -.
DR   PharmGKB; PA142670785; -.
DR   VEuPathDB; HostDB:ENSG00000164841; -.
DR   eggNOG; ENOG502QUYY; Eukaryota.
DR   GeneTree; ENSGT00530000063880; -.
DR   HOGENOM; CLU_079915_0_0_1; -.
DR   InParanoid; Q96NL1; -.
DR   OMA; GRKVCNC; -.
DR   OrthoDB; 5313320at2759; -.
DR   PhylomeDB; Q96NL1; -.
DR   TreeFam; TF335735; -.
DR   PathwayCommons; Q96NL1; -.
DR   SignaLink; Q96NL1; -.
DR   BioGRID-ORCS; 157753; 9 hits in 1149 CRISPR screens.
DR   ChiTaRS; TMEM74; human.
DR   GenomeRNAi; 157753; -.
DR   Pharos; Q96NL1; Tbio.
DR   PRO; PR:Q96NL1; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; Q96NL1; Protein.
DR   Bgee; ENSG00000164841; Expressed in secondary oocyte and 104 other cell types or tissues.
DR   ExpressionAtlas; Q96NL1; baseline and differential.
DR   GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR   GO; GO:0044325; F:transmembrane transporter binding; IEA:Ensembl.
DR   GO; GO:0051867; P:general adaptation syndrome, behavioral process; IEA:Ensembl.
DR   GO; GO:0016236; P:macroautophagy; IMP:HGNC.
DR   InterPro; IPR029695; TMEM74-like.
DR   PANTHER; PTHR16125; TRANSMEMBRANE PROTEIN 74; 1.
DR   PANTHER; PTHR16125:SF3; TRANSMEMBRANE PROTEIN 74; 1.
DR   Pfam; PF14927; Neurensin; 1.
DR   Genevisible; Q96NL1; HS.
PE   1: Evidence at protein level;
KW   Autophagy; Cytoplasmic vesicle; Lysosome; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..305
FT                   /note="Transmembrane protein 74"
FT                   /id="PRO_0000259600"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          52..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          123..143
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..79
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   305 AA;  33338 MW;  9521A382F126B683 CRC64;
     MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP RATEMEGSKL
     SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC NCCSQELETS FTYVDKNINL
     EQRNRSSPSA KGHNHPGELG WENPNEWSQE AAISLISEEE DDTSSEATSS GKSIDYGFIS
     AILFLVTGIL LVIISYIVPR EVTVDPNTVA AREMERLEKE SARLGAHLDR CVIAGLCLLT
     LGGVILSCLL MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNENT LELSLVEEDA
     LAVQS
//