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Protein

Transmembrane protein 74

Gene

TMEM74

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protein 74
Gene namesi
Name:TMEM74
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:26409. TMEM74.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei178 – 19821HelicalSequence AnalysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670785.

Polymorphism and mutation databases

BioMutaiTMEM74.
DMDMi74732587.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Transmembrane protein 74PRO_0000259600Add
BLAST

Proteomic databases

PaxDbiQ96NL1.
PRIDEiQ96NL1.

PTM databases

PhosphoSiteiQ96NL1.

Expressioni

Tissue specificityi

Expressed in heart, lung, and placenta.1 Publication

Gene expression databases

BgeeiQ96NL1.
CleanExiHS_TMEM74.
ExpressionAtlasiQ96NL1. baseline and differential.
GenevisibleiQ96NL1. HS.

Organism-specific databases

HPAiHPA015672.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FATE1Q969F03EBI-10292091,EBI-743099

Protein-protein interaction databases

BioGridi127621. 1 interaction.
IntActiQ96NL1. 1 interaction.
STRINGi9606.ENSP00000297459.

Structurei

3D structure databases

ProteinModelPortaliQ96NL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TMEM74 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47300.
GeneTreeiENSGT00530000063880.
HOGENOMiHOG000112021.
HOVERGENiHBG094072.
InParanoidiQ96NL1.
OMAiNEWSHEA.
OrthoDBiEOG7HF1KD.
PhylomeDBiQ96NL1.
TreeFamiTF335735.

Family and domain databases

InterProiIPR029696. Tmem74.
[Graphical view]
PANTHERiPTHR16125:SF3. PTHR16125:SF3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96NL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP
60 70 80 90 100
RATEMEGSKL SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC
110 120 130 140 150
NCCSQELETS FTYVDKNINL EQRNRSSPSA KGHNHPGELG WENPNEWSQE
160 170 180 190 200
AAISLISEEE DDTSSEATSS GKSIDYGFIS AILFLVTGIL LVIISYIVPR
210 220 230 240 250
EVTVDPNTVA AREMERLEKE SARLGAHLDR CVIAGLCLLT LGGVILSCLL
260 270 280 290 300
MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNENT LELSLVEEDA

LAVQS
Length:305
Mass (Da):33,338
Last modified:December 1, 2001 - v1
Checksum:i9521A382F126B683
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055230 mRNA. Translation: BAB70881.1.
BC030710 mRNA. Translation: AAH30710.1.
CCDSiCCDS6310.1.
RefSeqiNP_694560.1. NM_153015.1.
UniGeneiHs.99439.

Genome annotation databases

EnsembliENST00000297459; ENSP00000297459; ENSG00000164841.
GeneIDi157753.
KEGGihsa:157753.
UCSCiuc003ymy.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055230 mRNA. Translation: BAB70881.1.
BC030710 mRNA. Translation: AAH30710.1.
CCDSiCCDS6310.1.
RefSeqiNP_694560.1. NM_153015.1.
UniGeneiHs.99439.

3D structure databases

ProteinModelPortaliQ96NL1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127621. 1 interaction.
IntActiQ96NL1. 1 interaction.
STRINGi9606.ENSP00000297459.

PTM databases

PhosphoSiteiQ96NL1.

Polymorphism and mutation databases

BioMutaiTMEM74.
DMDMi74732587.

Proteomic databases

PaxDbiQ96NL1.
PRIDEiQ96NL1.

Protocols and materials databases

DNASUi157753.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297459; ENSP00000297459; ENSG00000164841.
GeneIDi157753.
KEGGihsa:157753.
UCSCiuc003ymy.1. human.

Organism-specific databases

CTDi157753.
GeneCardsiGC08M109619.
HGNCiHGNC:26409. TMEM74.
HPAiHPA015672.
MIMi613935. gene.
neXtProtiNX_Q96NL1.
PharmGKBiPA142670785.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG47300.
GeneTreeiENSGT00530000063880.
HOGENOMiHOG000112021.
HOVERGENiHBG094072.
InParanoidiQ96NL1.
OMAiNEWSHEA.
OrthoDBiEOG7HF1KD.
PhylomeDBiQ96NL1.
TreeFamiTF335735.

Miscellaneous databases

ChiTaRSiTMEM74. human.
GenomeRNAii157753.
NextBioi87542.
PROiQ96NL1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96NL1.
CleanExiHS_TMEM74.
ExpressionAtlasiQ96NL1. baseline and differential.
GenevisibleiQ96NL1. HS.

Family and domain databases

InterProiIPR029696. Tmem74.
[Graphical view]
PANTHERiPTHR16125:SF3. PTHR16125:SF3. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "TMEM74, a lysosome and autophagosome protein, regulates autophagy."
    Yu C., Wang L., Lv B., Lu Y., Zeng L., Chen Y., Ma D., Shi T., Wang L.
    Biochem. Biophys. Res. Commun. 369:622-629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene."
    He P., Peng Z., Luo Y., Wang L., Yu P., Deng W., An Y., Shi T., Ma D.
    Autophagy 5:52-60(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTMM74_HUMAN
AccessioniPrimary (citable) accession number: Q96NL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.