Q96NL1 (TMM74_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protein 74

Gene names

Name:

TMEM74

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. Ref.3 Ref.4
Subcellular location	Lysosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle › autophagosome membrane; Multi-pass membrane protein Ref.3.
Tissue specificity	Expressed in heart, lung, and placenta. Ref.3
Sequence similarities	Belongs to the TMEM74 family.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Cytoplasmic vesicle Lysosome Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	autophagic vacuole membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Transmembrane protein 74		PRO_0000259600
Regions
	Transmembrane	178 – 198	21	Helical; Potential
	Transmembrane	232 – 252	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96NL1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9521A382F126B683	FASTA	305	33,338
	10 20 30 40 50 60 MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP RATEMEGSKL 70 80 90 100 110 120 SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC NCCSQELETS FTYVDKNINL 130 140 150 160 170 180 EQRNRSSPSA KGHNHPGELG WENPNEWSQE AAISLISEEE DDTSSEATSS GKSIDYGFIS 190 200 210 220 230 240 AILFLVTGIL LVIISYIVPR EVTVDPNTVA AREMERLEKE SARLGAHLDR CVIAGLCLLT 250 260 270 280 290 300 LGGVILSCLL MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNENT LELSLVEEDA LAVQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"TMEM74, a lysosome and autophagosome protein, regulates autophagy." Yu C., Wang L., Lv B., Lu Y., Zeng L., Chen Y., Ma D., Shi T., Wang L. Biochem. Biophys. Res. Commun. 369:622-629(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene." He P., Peng Z., Luo Y., Wang L., Yu P., Deng W., An Y., Shi T., Ma D. Autophagy 5:52-60(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK055230 mRNA. Translation: BAB70881.1. BC030710 mRNA. Translation: AAH30710.1.
IPI	IPI00043733.
RefSeq	NP_694560.1. NM_153015.1.
UniGene	Hs.99439.
3D structure databases
ProteinModelPortal	Q96NL1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000297459.
PTM databases
PhosphoSite	Q96NL1.
Polymorphism databases
DMDM	74732587.
Proteomic databases
PaxDb	Q96NL1.
PRIDE	Q96NL1.
Protocols and materials databases
DNASU	157753.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297459; ENSP00000297459; ENSG00000164841.
GeneID	157753.
KEGG	hsa:157753.
UCSC	uc003ymy.1. human.
Organism-specific databases
CTD	157753.
GeneCards	GC08M109619.
HGNC	HGNC:26409. TMEM74.
HPA	HPA015672.
MIM	613935. gene.
neXtProt	NX_Q96NL1.
PharmGKB	PA142670785.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47300.
HOGENOM	HOG000112021.
HOVERGEN	HBG094072.
InParanoid	Q96NL1.
OMA	NEWSHEA.
OrthoDB	EOG498V1G.
PhylomeDB	Q96NL1.
Gene expression databases
Bgee	Q96NL1.
CleanEx	HS_TMEM74.
Genevestigator	Q96NL1.
GermOnline	ENSG00000164841. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	157753.
NextBio	87542.
SOURCE	Search...

Entry information

Entry name

TMM74_HUMAN

Accession

Primary (citable) accession number: Q96NL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.