Q96NI6 (LRFN5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-rich repeat and fibronectin type-III domain-containing protein 5

Gene names

Name:	LRFN5
Synonyms:	C14orf146, SALM5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca2+-independent manner. Promotes neurite outgrowth in hippocampal neurons. Ref.5 Ref.6
Subunit structure	Can form heteromeric complexes with LRFN1, LRFN2, LRFN3 and LFRN4. Able to form homomeric complexes across cell junctions, between adjacent cells. Does not interact with DLG1, DLG2, DLG3 and DLG4.
Subcellular location	Membrane By similarity; Single-pass type I membrane protein Potential.
Domain	Lacks a cytoplasmic PDZ-binding motif, which has been implicated in function of related LRFN proteins.
Sequence similarities	Belongs to the LRFN family. Contains 1 fibronectin type-III domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 7 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 719	702	Leucine-rich repeat and fibronectin type-III domain-containing protein 5		PRO_0000014845
Regions
	Topological domain	18 – 529	512	Extracellular Potential
	Transmembrane	530 – 550	21	Helical; Potential
	Topological domain	551 – 719	169	Cytoplasmic Potential
	Domain	18 – 51	34	LRRNT
	Repeat	52 – 73	22	LRR 1
	Repeat	76 – 97	22	LRR 2
	Repeat	100 – 121	22	LRR 3
	Repeat	124 – 145	22	LRR 4
	Repeat	148 – 169	22	LRR 5
	Repeat	172 – 193	22	LRR 6
	Repeat	196 – 217	22	LRR 7
	Domain	240 – 286	47	LRRCT
	Domain	287 – 373	87	Ig-like
	Domain	414 – 503	90	Fibronectin type-III
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential
	Glycosylation	406	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Disulfide bond	308 ↔ 357		By similarity
Experimental info
	Sequence conflict	544	1	F → L in BAG53340. Ref.1
	Sequence conflict	679	1	A → V in BAB70910. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96NI6 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: DD92951A9705FF4B	FASTA	719	79,445
	10 20 30 40 50 60 MEKILFYLFL IGIAVKAQIC PKRCVCQILS PNLATLCAKK GLLFVPPNID RRTVELRLAD 70 80 90 100 110 120 NFVTNIKRKD FANMTSLVDL TLSRNTISFI TPHAFADLRN LRALHLNSNR LTKITNDMFS 130 140 150 160 170 180 GLSNLHHLIL NNNQLTLISS TAFDDVFALE ELDLSYNNLE TIPWDAVEKM VSLHTLSLDH 190 200 210 220 230 240 NMIDNIPKGT FSHLHKMTRL DVTSNKLQKL PPDPLFQRAQ VLATSGIISP STFALSFGGN 250 260 270 280 290 300 PLHCNCELLW LRRLSREDDL ETCASPPLLT GRYFWSIPEE EFLCEPPLIT RHTHEMRVLE 310 320 330 340 350 360 GQRATLRCKA RGDPEPAIHW ISPEGKLISN ATRSLVYDNG TLDILITTVK DTGAFTCIAS 370 380 390 400 410 420 NPAGEATQIV DLHIIKLPHL LNSTNHIHEP DPGSSDISTS TKSGSNTSSS NGDTKLSQDK 430 440 450 460 470 480 IVVAEATSST ALLKFNFQRN IPGIRMFQIQ YNGTYDDTLV YRMIPPTSKT FLVNNLAAGT 490 500 510 520 530 540 MYDLCVLAIY DDGITSLTAT RVVGCIQFTT EQDYVRCHFM QSQFLGGTMI IIIGGIIVAS 550 560 570 580 590 600 VLVFIIILMI RYKVCNNNGQ HKVTKVSNVY SQTNGAQIQG CSVTLPQSVS KQAVGHEENA 610 620 630 640 650 660 QCCKATSDNV IQSSETCSSQ DSSTTTSALP PSWTSSTSVS QKQKRKTGTK PSTEPQNEAV 670 680 690 700 710 TNVESQNTNR NNSTALQLAS RPPDSVTEGP TSKRAHIKPN ALLTNVDQIV QETQRLELI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal brain.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses." Ko J., Kim S., Chung H.S., Kim K., Han K., Kim H., Jun H., Kaang B.-K., Kim E. Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF INTERACTION WITH DLG1; DLG2; DLG3 AND DLG4.
[5]	"The SALM family of adhesion-like molecules forms heteromeric and homomeric complexes." Seabold G.K., Wang P.Y., Chang K., Wang C.Y., Wang Y.X., Petralia R.S., Wenthold R.J. J. Biol. Chem. 283:8395-8405(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH LRFN1; LRFN2; LRFN3; LRFN4 AND LRFN5.
[6]	"Synaptic adhesion-like molecules (SALMs) promote neurite outgrowth." Wang P.Y., Seabold G.K., Wenthold R.J. Mol. Cell. Neurosci. 39:83-94(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK055365 mRNA. Translation: BAB70910.1. AK096627 mRNA. Translation: BAG53340.1. AL138498 Genomic DNA. No translation available. BC043165 mRNA. Translation: AAH43165.1.
IPI	IPI00396961.
RefSeq	NP_689660.2. NM_152447.3.
UniGene	Hs.136893.
3D structure databases
ProteinModelPortal	Q96NI6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000298119.
PTM databases
PhosphoSite	Q96NI6.
Polymorphism databases
DMDM	116242620.
Proteomic databases
PaxDb	Q96NI6.
PRIDE	Q96NI6.
Protocols and materials databases
DNASU	145581.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000298119; ENSP00000298119; ENSG00000165379.
GeneID	145581.
KEGG	hsa:145581.
UCSC	uc001wvm.3. human.
Organism-specific databases
CTD	145581.
GeneCards	GC14P042076.
HGNC	HGNC:20360. LRFN5.
HPA	HPA001177.
MIM	612811. gene.
neXtProt	NX_Q96NI6.
PharmGKB	PA134888453.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249839.
HOGENOM	HOG000237343.
HOVERGEN	HBG052352.
InParanoid	Q96NI6.
KO	K16358.
OMA	NAQCCKA.
OrthoDB	EOG4X6C81.
PhylomeDB	Q96NI6.
Gene expression databases
ArrayExpress	Q96NI6.
Bgee	Q96NI6.
CleanEx	HS_LRFN5.
Genevestigator	Q96NI6.
GermOnline	ENSG00000165379. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR003961. Fibronectin_type3. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003598. Ig_sub2. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR026879. Lrfn5. IPR000372. LRR-contain_N. IPR026906. LRR_5. [Graphical view]
PANTHER	PTHR24373:SF3. PTHR24373:SF3. 1 hit.
Pfam	PF07679. I-set. 1 hit. PF13306. LRR_5. 1 hit. [Graphical view]
SMART	SM00408. IGc2. 1 hit. SM00369. LRR_TYP. 3 hits. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 1 hit.
PROSITE	PS50853. FN3. 1 hit. PS50835. IG_LIKE. 1 hit. PS51450. LRR. 6 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	145581.
NextBio	85141.
SOURCE	Search...

Entry information

Entry name

LRFN5_HUMAN

Accession

Primary (citable) accession number: Q96NI6
Secondary accession number(s): B3KU78, Q86XL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.