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Protein

AP-4 complex accessory subunit tepsin

Gene

ENTHD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex accessory subunit tepsin
Alternative name(s):
ENTH domain-containing protein 2
Epsin for AP-4
Tetra-epsin
Gene namesi
Name:ENTHD2
Synonyms:C17orf56
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:26458. ENTHD2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672239.

Polymorphism and mutation databases

BioMutaiENTHD2.
DMDMi74732479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525AP-4 complex accessory subunit tepsinPRO_0000286678Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei333 – 3331PhosphoserineCombined sources
Modified residuei356 – 3561PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96N21.
MaxQBiQ96N21.
PaxDbiQ96N21.
PRIDEiQ96N21.

PTM databases

iPTMnetiQ96N21.
PhosphoSiteiQ96N21.

Expressioni

Gene expression databases

BgeeiQ96N21.
CleanExiHS_C17orf56.
ExpressionAtlasiQ96N21. baseline and differential.
GenevisibleiQ96N21. HS.

Interactioni

Subunit structurei

Associates with the adapter-like complex 4 (AP-4). AP-4 is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Within this complex, may directly interact with AP4B1.

Protein-protein interaction databases

BioGridi127002. 29 interactions.
IntActiQ96N21. 29 interactions.
STRINGi9606.ENSP00000300714.

Structurei

3D structure databases

ProteinModelPortaliQ96N21.
SMRiQ96N21. Positions 24-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 141134ENTHAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi193 – 22533Ser-richAdd
BLAST
Compositional biasi365 – 43167Pro-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IEKN. Eukaryota.
ENOG410ZYSQ. LUCA.
GeneTreeiENSGT00390000015076.
HOGENOMiHOG000049246.
HOVERGENiHBG064281.
InParanoidiQ96N21.
OMAiPGSSQCL.
OrthoDBiEOG70S759.
PhylomeDBiQ96N21.
TreeFamiTF331354.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96N21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPPLRDR LSFLHRLPIL LKGTSDDDVP CPGYLFEEIA KISHESPGSS
60 70 80 90 100
QCLLEYLLSR LHSSSGHGKL KVLKILLYLC SHGSSFFLLI LKRNSAFIQE
110 120 130 140 150
AAAFAGPPDP LHGNSLYQKV RAAAQDLGST LFSDTVLPLA PSQPLGTPPA
160 170 180 190 200
TGMGSQARPH STLQGFGYSK EHGRTAVRHQ PGQAGGGWDE LDSGPSSQNS
210 220 230 240 250
SQNSDLSRVS DSGSHSGSDS HSGASREPGD LAERVEVVAL SDCQQELSLV
260 270 280 290 300
RTVTRGPRAF LSREEAQHFI KACGLLNCEA VLQLLTCHLR GTSECTQLRA
310 320 330 340 350
LCAIASLGSS DLLPQEHILL RTRPWLQELS MGSPGPVTNK ATKILRHFEA
360 370 380 390 400
SCGQLSPARG TSAEPGPTAA LPGPSDLLTD AVPLPGSQVF LQPLSSTPVS
410 420 430 440 450
SRSPAPSSGM PSSPVPTPPP DASPIPAPGD PSEAEARLAE SRRWRPERIP
460 470 480 490 500
GGTDSPKRGP SSCAWSRDSL FAGMELVACP RLVGAGAAAG ESCPDAPRAP
510 520
QTSSQRTAAK EPPGSEPSAF AFLNA
Length:525
Mass (Da):55,137
Last modified:December 1, 2001 - v1
Checksum:iB79970A37B801549
GO
Isoform 2 (identifier: Q96N21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-175: FFLLILKRNS...FGYSKEHGRT → MGSQARPHST...LLPGAIPGPR

Show »
Length:441
Mass (Da):45,755
Checksum:i29679B1DBB77B6E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q96N21-2)
Sequence conflicti22 – 221R → H in BAC87593 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8585Missing in isoform 2. 1 PublicationVSP_025141Add
BLAST
Alternative sequencei86 – 17590FFLLI…EHGRT → MGSQARPHSTLQGFGYSKEH GRTGSAGEAFLSTIQKAAEV VASAMRPGPESPSTRRLLPR GDTYQPAMMPSASHGPPTLG NLLPGAIPGPR in isoform 2. 1 PublicationVSP_025142Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056090 mRNA. Translation: BAB71091.1.
AK127221 mRNA. Translation: BAC86888.1.
AK128728 mRNA. Translation: BAC87593.1.
BC064483 mRNA. Translation: AAH64483.1.
CCDSiCCDS11779.1. [Q96N21-1]
RefSeqiNP_653280.1. NM_144679.2. [Q96N21-1]
XP_006721775.1. XM_006721712.2. [Q96N21-2]
UniGeneiHs.631761.

Genome annotation databases

EnsembliENST00000300714; ENSP00000300714; ENSG00000167302. [Q96N21-1]
GeneIDi146705.
KEGGihsa:146705.
UCSCiuc002jzu.2. human. [Q96N21-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056090 mRNA. Translation: BAB71091.1.
AK127221 mRNA. Translation: BAC86888.1.
AK128728 mRNA. Translation: BAC87593.1.
BC064483 mRNA. Translation: AAH64483.1.
CCDSiCCDS11779.1. [Q96N21-1]
RefSeqiNP_653280.1. NM_144679.2. [Q96N21-1]
XP_006721775.1. XM_006721712.2. [Q96N21-2]
UniGeneiHs.631761.

3D structure databases

ProteinModelPortaliQ96N21.
SMRiQ96N21. Positions 24-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127002. 29 interactions.
IntActiQ96N21. 29 interactions.
STRINGi9606.ENSP00000300714.

PTM databases

iPTMnetiQ96N21.
PhosphoSiteiQ96N21.

Polymorphism and mutation databases

BioMutaiENTHD2.
DMDMi74732479.

Proteomic databases

EPDiQ96N21.
MaxQBiQ96N21.
PaxDbiQ96N21.
PRIDEiQ96N21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300714; ENSP00000300714; ENSG00000167302. [Q96N21-1]
GeneIDi146705.
KEGGihsa:146705.
UCSCiuc002jzu.2. human. [Q96N21-1]

Organism-specific databases

CTDi146705.
GeneCardsiENTHD2.
H-InvDBHIX0014244.
HIX0014245.
HGNCiHGNC:26458. ENTHD2.
neXtProtiNX_Q96N21.
PharmGKBiPA142672239.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEKN. Eukaryota.
ENOG410ZYSQ. LUCA.
GeneTreeiENSGT00390000015076.
HOGENOMiHOG000049246.
HOVERGENiHBG064281.
InParanoidiQ96N21.
OMAiPGSSQCL.
OrthoDBiEOG70S759.
PhylomeDBiQ96N21.
TreeFamiTF331354.

Miscellaneous databases

GenomeRNAii146705.
PROiQ96N21.

Gene expression databases

BgeeiQ96N21.
CleanExiHS_C17orf56.
ExpressionAtlasiQ96N21. baseline and differential.
GenevisibleiQ96N21. HS.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Hippocampus, Teratocarcinoma and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333 AND SER-356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles."
    Borner G.H., Antrobus R., Hirst J., Bhumbra G.S., Kozik P., Jackson L.P., Sahlender D.A., Robinson M.S.
    J. Cell Biol. 197:141-160(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP4B1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAP4AT_HUMAN
AccessioniPrimary (citable) accession number: Q96N21
Secondary accession number(s): Q6ZQU0, Q6ZSQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.