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Protein

AP-4 complex accessory subunit Tepsin

Gene

TEPSIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network.2 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex accessory subunit TepsinCurated
Alternative name(s):
ENTH domain-containing protein 2Imported
Epsin for AP-4
Tetra-epsin1 Publication
Gene namesi
Name:TEPSIN1 PublicationImported
Synonyms:C17orf56Imported, ENTHD2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000167302.9.
HGNCiHGNC:26458. TEPSIN.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi467R → A: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi468D → A: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi469S → A or P: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi470L → A: Loss of interaction with AP4B1 n vitro. 1 Publication1
Mutagenesisi470L → S: Loss of interaction with AP4B1 in vitro; when associated with S-471. 1 Publication1
Mutagenesisi471F → A: Loss of interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi471F → S: Loss of interaction with AP4B1 in vitro; when associated with S-470. 1 Publication1
Mutagenesisi472A → D or P: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi473G → A: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi473G → I: Loss of interaction with AP4B1 in vitro; when associated with Q-474. 1 Publication1
Mutagenesisi474M → A: Decreased interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi474M → D: Decreased interaction with AP4B1 in vitro; when associated with A-476. 1 Publication1
Mutagenesisi474M → Q: Loss of interaction with AP4B1 in vitro; when associated with I-473. 1 Publication1
Mutagenesisi475E → A: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi476L → A: No effect on interaction with AP4B1 in vitro. Decreased interaction with AP4B1; when associated with D-474. 2 Publications1
Mutagenesisi476L → S: Decreased interaction with AP4B1 in vitro; when associated with S-477. 1 Publication1
Mutagenesisi477V → A: No effect on interaction with AP4B1 in vitro. 1 Publication1
Mutagenesisi477V → S: Decreased interaction with AP4B1 in vitro; when associated with S-476. 1 Publication1
Mutagenesisi516E → A: No effect on interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi517P → A: No effect on interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi518S → A: Loss of interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi519A → D: Loss of interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi520F → A: Loss of interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi521A → D: No effect on interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi522F → A: Loss of interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi523L → A: Loss of interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi524N → A: Decreased interaction with AP4E1 in vitro. 1 Publication1
Mutagenesisi525A → D: No effect on interaction with AP4E1 in vitro. 1 Publication1

Organism-specific databases

DisGeNETi146705.
OpenTargetsiENSG00000167302.
PharmGKBiPA142672239.

Polymorphism and mutation databases

BioMutaiENTHD2.
DMDMi74732479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002866781 – 525AP-4 complex accessory subunit TepsinAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei333PhosphoserineCombined sources1
Modified residuei356PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96N21.
MaxQBiQ96N21.
PaxDbiQ96N21.
PeptideAtlasiQ96N21.
PRIDEiQ96N21.

PTM databases

iPTMnetiQ96N21.
PhosphoSitePlusiQ96N21.

Expressioni

Gene expression databases

BgeeiENSG00000167302.
CleanExiHS_C17orf56.
ExpressionAtlasiQ96N21. baseline and differential.
GenevisibleiQ96N21. HS.

Organism-specific databases

HPAiHPA063410.

Interactioni

Subunit structurei

Interacts with AP4B1 and AP4E1; the interaction is direct and mediates the association of TEPSIN with the adapter-like complex 4 (AP-4), a heterotetramer composed of AP4B1, AP4E1, AP4M1 and AP4S1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi127002. 37 interactors.
IntActiQ96N21. 51 interactors.
STRINGi9606.ENSP00000300714.

Structurei

Secondary structure

1525
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 24Combined sources18
Beta strandi26 – 29Combined sources4
Helixi35 – 45Combined sources11
Helixi47 – 63Combined sources17
Helixi66 – 82Combined sources17
Helixi85 – 93Combined sources9
Helixi95 – 102Combined sources8
Turni110 – 112Combined sources3
Helixi115 – 131Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WF9X-ray1.80A1-153[»]
5WFBX-ray1.38A/B1-140[»]
ProteinModelPortaliQ96N21.
SMRiQ96N21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 141ENTHAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni467 – 477Interaction with AP4B12 PublicationsAdd BLAST11
Regioni515 – 525Interaction with AP4E11 PublicationAdd BLAST11

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi193 – 225Ser-richAdd BLAST33
Compositional biasi365 – 431Pro-richAdd BLAST67

Phylogenomic databases

eggNOGiENOG410IEKN. Eukaryota.
ENOG410ZYSQ. LUCA.
GeneTreeiENSGT00390000015076.
HOGENOMiHOG000049246.
HOVERGENiHBG064281.
InParanoidiQ96N21.
OrthoDBiEOG091G03PK.
PhylomeDBiQ96N21.
TreeFamiTF331354.

Family and domain databases

CDDicd03572. ENTH_epsin_related. 1 hit.
Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR013809. ENTH.
IPR035802. ENTH/VHS_tepsin.
IPR008942. ENTH_VHS.
PfamiView protein in Pfam
PF01417. ENTH. 1 hit.
SUPFAMiSSF48464. SSF48464. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96N21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPPLRDR LSFLHRLPIL LKGTSDDDVP CPGYLFEEIA KISHESPGSS
60 70 80 90 100
QCLLEYLLSR LHSSSGHGKL KVLKILLYLC SHGSSFFLLI LKRNSAFIQE
110 120 130 140 150
AAAFAGPPDP LHGNSLYQKV RAAAQDLGST LFSDTVLPLA PSQPLGTPPA
160 170 180 190 200
TGMGSQARPH STLQGFGYSK EHGRTAVRHQ PGQAGGGWDE LDSGPSSQNS
210 220 230 240 250
SQNSDLSRVS DSGSHSGSDS HSGASREPGD LAERVEVVAL SDCQQELSLV
260 270 280 290 300
RTVTRGPRAF LSREEAQHFI KACGLLNCEA VLQLLTCHLR GTSECTQLRA
310 320 330 340 350
LCAIASLGSS DLLPQEHILL RTRPWLQELS MGSPGPVTNK ATKILRHFEA
360 370 380 390 400
SCGQLSPARG TSAEPGPTAA LPGPSDLLTD AVPLPGSQVF LQPLSSTPVS
410 420 430 440 450
SRSPAPSSGM PSSPVPTPPP DASPIPAPGD PSEAEARLAE SRRWRPERIP
460 470 480 490 500
GGTDSPKRGP SSCAWSRDSL FAGMELVACP RLVGAGAAAG ESCPDAPRAP
510 520
QTSSQRTAAK EPPGSEPSAF AFLNA
Length:525
Mass (Da):55,137
Last modified:December 1, 2001 - v1
Checksum:iB79970A37B801549
GO
Isoform 2 (identifier: Q96N21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-175: FFLLILKRNS...FGYSKEHGRT → MGSQARPHST...LLPGAIPGPR

Show »
Length:441
Mass (Da):45,755
Checksum:i29679B1DBB77B6E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q96N21-2)
Sequence conflicti22R → H in BAC87593 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0251411 – 85Missing in isoform 2. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_02514286 – 175FFLLI…EHGRT → MGSQARPHSTLQGFGYSKEH GRTGSAGEAFLSTIQKAAEV VASAMRPGPESPSTRRLLPR GDTYQPAMMPSASHGPPTLG NLLPGAIPGPR in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056090 mRNA. Translation: BAB71091.1.
AK127221 mRNA. Translation: BAC86888.1.
AK128728 mRNA. Translation: BAC87593.1.
BC064483 mRNA. Translation: AAH64483.1.
CCDSiCCDS11779.1. [Q96N21-1]
RefSeqiNP_653280.1. NM_144679.2. [Q96N21-1]
XP_006721775.1. XM_006721712.2. [Q96N21-2]
UniGeneiHs.631761.

Genome annotation databases

EnsembliENST00000300714; ENSP00000300714; ENSG00000167302. [Q96N21-1]
GeneIDi146705.
KEGGihsa:146705.
UCSCiuc002jzu.2. human. [Q96N21-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAP4AT_HUMAN
AccessioniPrimary (citable) accession number: Q96N21
Secondary accession number(s): Q6ZQU0, Q6ZSQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: November 22, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references