Q96MU8 (KREM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kremen protein 1

Alternative name(s):
Dickkopf receptor
Kringle domain-containing transmembrane protein 1
Kringle-containing protein marking the eye and the nose

Gene names

Name:	KREMEN1
Synonyms:	KREMEN, KRM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Contains 1 CUB domain. Contains 1 kringle domain. Contains 1 WSC domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Kringle Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell communication Traceable author statement PubMed 11267660. Source: UniProtKB regulation of canonical Wnt receptor signaling pathway Non-traceable author statement PubMed 17804805. Source: BHF-UCL
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Traceable author statement PubMed 11267660. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MU8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available. Exon 1 splicing donor site is not canonical.

Isoform 2 (identifier: Q96MU8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     471-473: VSD → AIQDSEVTSLIWSQGQPRSI

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96MU8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     373-389: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 473	454	Kremen protein 1		PRO_0000021564
Regions
	Topological domain	21 – 392	372	Extracellular Potential
	Transmembrane	393 – 413	21	Helical; Potential
	Topological domain	414 – 473	60	Cytoplasmic Potential
	Domain	31 – 114	84	Kringle
	Domain	116 – 210	95	WSC
	Domain	214 – 321	108	CUB
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 114		By similarity
	Disulfide bond	55 ↔ 95		By similarity
	Disulfide bond	84 ↔ 109		By similarity
	Disulfide bond	214 ↔ 240		By similarity
Natural variations
	Alternative sequence	31	1	E → GPE in isoform 2 and isoform 3.		VSP_034914
	Alternative sequence	373 – 389	17	Missing in isoform 3.		VSP_015698
	Alternative sequence	471 – 473	3	VSD → AIQDSEVTSLIWSQGQPRSI in isoform 2.		VSP_003900
Experimental info
	Sequence conflict	204	1	I → V in BAB71180. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: F6D30DDE708C186B	FASTA	473	51,744
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP ECFTANGADY RGTQNWTALQ GGKPCLFWNE 70 80 90 100 110 120 TFQHPYNTLK YPNGEGGLGE HNYCRNPDGD VSPWCYVAEH EDGVYWKYCE IPACQMPGNL 130 140 150 160 170 180 GCYKDHGNPP PLTGTSKTSN KLTIQTCISF CRSQRFKFAG MESGYACFCG NNPDYWKYGE 190 200 210 220 230 240 AASTECNSVC FGDHTQPCGG DGRIILFDTL VGACGGNYSA MSSVVYSPDF PDTYATGRVC 250 260 270 280 290 300 YWTIRVPGAS HIHFSFPLFD IRDSADMVEL LDGYTHRVLA RFHGRSRPPL SFNVSLDFVI 310 320 330 340 350 360 LYFFSDRINQ AQGFAVLYQA VKEELPQERP AVNQTVAEVI TEQANLSVSA ARSSKVLYVI 370 380 390 400 410 420 TTSPSHPPQT VPGSNSWAPP MGAGSHRVEG WTVYGLATLL ILTVTAIVAK ILLHVTFKSH 430 440 450 460 470 RVPASGDLRD CHQPGTSGEI WSIFYKPSTS ISIFKKKLKG QSQQDDRNPL VSD
	Isoform 2 [UniParc]. Checksum: BCCDBC62C07AFF56	FASTA	492	53,795
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW 70 80 90 100 110 120 NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG 130 140 150 160 170 180 NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY 190 200 210 220 230 240 GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR 250 260 270 280 290 300 VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF 310 320 330 340 350 360 VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY 370 380 390 400 410 420 VITTSPSHPP QTVPGSNSWA PPMGAGSHRV EGWTVYGLAT LLILTVTAIV AKILLHVTFK 430 440 450 460 470 480 SHRVPASGDL RDCHQPGTSG EIWSIFYKPS TSISIFKKKL KGQSQQDDRN PLAIQDSEVT 490 SLIWSQGQPR SI
	Isoform 3 [UniParc]. Checksum: 8A56506A033E1D4C	FASTA	458	50,177
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW 70 80 90 100 110 120 NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG 130 140 150 160 170 180 NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY 190 200 210 220 230 240 GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR 250 260 270 280 290 300 VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF 310 320 330 340 350 360 VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY 370 380 390 400 410 420 VITTSPSHPP QTVPGWTVYG LATLLILTVT AIVAKILLHV TFKSHRVPAS GDLRDCHQPG 430 440 450 TSGEIWSIFY KPSTSISIFK KKLKGQSQQD DRNPLVSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nakamura T., Nakamura T. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Eye.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB059618 mRNA. Translation: BAB40969.1. AK056425 mRNA. Translation: BAB71180.1. AL021393, Z95116 Genomic DNA. Translation: CAM25378.1. AL021393, Z95116 Genomic DNA. Translation: CAQ07689.1. AL021393, Z95116 Genomic DNA. Translation: CAQ07690.1. Z95116, AL021393 Genomic DNA. Translation: CAM25375.1. Z95116, AL021393 Genomic DNA. Translation: CAQ08609.1. Z95116, AL021393 Genomic DNA. Translation: CAQ08610.1. CH471095 Genomic DNA. Translation: EAW59774.1. BC063787 mRNA. Translation: AAH63787.1.
IPI	IPI00140177. IPI00218929. IPI00651704.
RefSeq	NP_001034659.2. NM_001039570.2. NP_114434.3. NM_032045.4.
UniGene	Hs.229335.
3D structure databases
ProteinModelPortal	Q96MU8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46462N.
PTM databases
PhosphoSite	Q96MU8.
Polymorphism databases
DMDM	212287927.
Proteomic databases
PaxDb	Q96MU8.
PRIDE	Q96MU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327813; ENSP00000331242; ENSG00000183762. ENST00000400335; ENSP00000383189; ENSG00000183762. ENST00000407188; ENSP00000385431; ENSG00000183762.
GeneID	83999.
KEGG	hsa:83999.
UCSC	uc003ael.3. human. uc011akm.1. human. uc011akn.2. human.
Organism-specific databases
CTD	83999.
GeneCards	GC22P029469.
HGNC	HGNC:17550. KREMEN1.
MIM	609898. gene.
neXtProt	NX_Q96MU8.
PharmGKB	PA38241.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG262750.
HOVERGEN	HBG052290.
OMA	HRVLVRF.
Gene expression databases
ArrayExpress	Q96MU8.
Bgee	Q96MU8.
Genevestigator	Q96MU8.
GermOnline	ENSG00000183762. Homo sapiens.
Family and domain databases
Gene3D	2.40.20.10. 1 hit. 2.60.120.290. 1 hit.
InterPro	IPR000859. CUB_dom. IPR017076. Kremen. IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR002889. WSC_carb-bd. [Graphical view]
Pfam	PF00431. CUB. 1 hit. PF00051. Kringle. 1 hit. PF01822. WSC. 1 hit. [Graphical view]
PIRSF	PIRSF036961. Kremen. 1 hit.
SMART	SM00042. CUB. 1 hit. SM00130. KR. 1 hit. [Graphical view]
SUPFAM	SSF49854. CUB. 1 hit. SSF57440. Kringle-like. 1 hit.
PROSITE	PS01180. CUB. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS51212. WSC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	KREMEN1. human.
GenomeRNAi	83999.
NextBio	73160.
SOURCE	Search...

Entry information

Entry name

KREM1_HUMAN

Accession

Primary (citable) accession number: Q96MU8
Secondary accession number(s): B0QY46 , B0QY47, B1AJR5, Q5TIB9, Q6P3X6, Q9BY70, Q9UGS5, Q9UGU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.