Reviewed, UniProtKB/Swiss-Prot Q96MU8 (KREM1_HUMAN)

Names and origin

Protein names

Recommended name:
    Kremen protein 1
Alternative name(s):
    Kringle-containing protein marking the eye and the nose
    Kringle domain-containing transmembrane protein 1
    Dickkopf receptor

Gene names

Name:	KREMEN1
Synonyms:	KREMEN, KRM1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Contains 1 CUB domain. Contains 1 kringle domain. Contains 1 WSC domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Kringle Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MU8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96MU8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     471-473: VSD → AIQDSEVTSLIWSQGQPRSI

Note: No experimental confirmation available.

Isoform 3 (identifier: Q96MU8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     373-389: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 473	454	Kremen protein 1		PRO_0000021564
Regions
	Topological domain	21 – 392	372	Extracellular Potential
	Transmembrane	393 – 413	21	Potential
	Topological domain	414 – 473	60	Cytoplasmic Potential
	Domain	31 – 114	84	Kringle
	Domain	116 – 210	95	WSC
	Domain	214 – 321	108	CUB
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 114		By similarity
	Disulfide bond	55 ↔ 95		By similarity
	Disulfide bond	84 ↔ 109		By similarity
	Disulfide bond	214 ↔ 240		By similarity
Natural variations
	Alternative sequence	31	1	E → GPE in isoform 2 and isoform 3.		VSP_034914
	Alternative sequence	373 – 389	17	Missing in isoform 3.		VSP_015698
	Alternative sequence	471 – 473	3	VSD → AIQDSEVTSLIWSQGQPRSI in isoform 2.		VSP_003900
Experimental info
	Sequence conflict	204	1	I → V in BAB71180. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: F6D30DDE708C186B	FASTA	473	51,744
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP ECFTANGADY RGTQNWTALQ GGKPCLFWNE 70 80 90 100 110 120 TFQHPYNTLK YPNGEGGLGE HNYCRNPDGD VSPWCYVAEH EDGVYWKYCE IPACQMPGNL 130 140 150 160 170 180 GCYKDHGNPP PLTGTSKTSN KLTIQTCISF CRSQRFKFAG MESGYACFCG NNPDYWKYGE 190 200 210 220 230 240 AASTECNSVC FGDHTQPCGG DGRIILFDTL VGACGGNYSA MSSVVYSPDF PDTYATGRVC 250 260 270 280 290 300 YWTIRVPGAS HIHFSFPLFD IRDSADMVEL LDGYTHRVLA RFHGRSRPPL SFNVSLDFVI 310 320 330 340 350 360 LYFFSDRINQ AQGFAVLYQA VKEELPQERP AVNQTVAEVI TEQANLSVSA ARSSKVLYVI 370 380 390 400 410 420 TTSPSHPPQT VPGSNSWAPP MGAGSHRVEG WTVYGLATLL ILTVTAIVAK ILLHVTFKSH 430 440 450 460 470 RVPASGDLRD CHQPGTSGEI WSIFYKPSTS ISIFKKKLKG QSQQDDRNPL VSD
	Isoform 2. Checksum: BCCDBC62C07AFF56	FASTA	492	53,795
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW 70 80 90 100 110 120 NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG 130 140 150 160 170 180 NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY 190 200 210 220 230 240 GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR 250 260 270 280 290 300 VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF 310 320 330 340 350 360 VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY 370 380 390 400 410 420 VITTSPSHPP QTVPGSNSWA PPMGAGSHRV EGWTVYGLAT LLILTVTAIV AKILLHVTFK 430 440 450 460 470 480 SHRVPASGDL RDCHQPGTSG EIWSIFYKPS TSISIFKKKL KGQSQQDDRN PLAIQDSEVT 490 SLIWSQGQPR SI
	Isoform 3. Checksum: 8A56506A033E1D4C	FASTA	458	50,177
	10 20 30 40 50 60 MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW 70 80 90 100 110 120 NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG 130 140 150 160 170 180 NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY 190 200 210 220 230 240 GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR 250 260 270 280 290 300 VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF 310 320 330 340 350 360 VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY 370 380 390 400 410 420 VITTSPSHPP QTVPGWTVYG LATLLILTVT AIVAKILLHV TFKSHRVPAS GDLRDCHQPG 430 440 450 TSGEIWSIFY KPSTSISIFK KKLKGQSQQD DRNPLVSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nakamura T., Nakamura T. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB059618 mRNA. Translation: BAB40969.1. AK056425 mRNA. Translation: BAB71180.1. AL021393, Z95116 Genomic DNA. Translation: CAM25378.1. AL021393, Z95116 Genomic DNA. Translation: CAQ07689.1. AL021393, Z95116 Genomic DNA. Translation: CAQ07690.1. Z95116, AL021393 Genomic DNA. Translation: CAM25375.1. Z95116, AL021393 Genomic DNA. Translation: CAQ08609.1. Z95116, AL021393 Genomic DNA. Translation: CAQ08610.1. CH471095 Genomic DNA. Translation: EAW59774.1. BC063787 mRNA. Translation: AAH63787.1.
IPI	IPI00140177. IPI00218929. IPI00651704.
RefSeq	NP_114434.3.
UniGene	Hs.229335
3D structure databases
HSSP	HSSP built from PDB template 1PML based on UniProtKB P00750.
ModBase	Search...
Proteomic databases
PRIDE	Q96MU8.
Genome annotation databases
Ensembl	ENSG00000183762. Homo sapiens. [Contig view]
GeneID	83999.
KEGG	hsa:83999.
Organism-specific databases
GeneCards	GC22P027793.
HGNC	HGNC:17550. KREMEN1.
MIM	609898. gene.
PharmGKB	PA38241.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96MU8.
HOVERGEN	Q96MU8.
OMA	Q96MU8. GAGSHRV.
Gene expression databases
ArrayExpress	Q96MU8.
Bgee	Q96MU8.
GermOnline	ENSG00000183762. Homo sapiens.
Family and domain databases
InterPro	IPR000859. CUB. IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR002889. WSC_carb_bd. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 1 hit. G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00431. CUB. 1 hit. PF00051. Kringle. 1 hit. PF01822. WSC. 1 hit. [Graphical view]
PRINTS	PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00042. CUB. 1 hit. SM00130. KR. 1 hit. [Graphical view]
PROSITE	PS01180. CUB. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS51212. WSC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	73160.
SOURCE	Search...

Entry information

Entry name

KREM1_HUMAN

Accession

Primary (citable) accession number: Q96MU8
Secondary accession number(s): B0QY46 , B0QY47, B1AJR5, Q5TIB9, Q6P3X6, Q9BY70, Q9UGS5, Q9UGU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)