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Protein

Kremen protein 1

Gene

KREMEN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity).By similarity

GO - Biological processi

  • cell communication Source: UniProtKB
  • regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_264383. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_264594. misspliced LRP5 mutants have enhanced beta-catenin-dependent signaling.
REACT_264596. TCF dependent signaling in response to WNT.
SignaLinkiQ96MU8.

Names & Taxonomyi

Protein namesi
Recommended name:
Kremen protein 1
Alternative name(s):
Dickkopf receptor
Kringle domain-containing transmembrane protein 1
Kringle-containing protein marking the eye and the nose
Gene namesi
Name:KREMEN1
Synonyms:KREMEN, KRM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:17550. KREMEN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 392372ExtracellularSequence AnalysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 47360CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38241.

Polymorphism and mutation databases

BioMutaiKREMEN1.
DMDMi212287927.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 473454Kremen protein 1PRO_0000021564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 114By similarity
Glycosylationi45 – 451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi55 ↔ 95By similarity
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi84 ↔ 109By similarity
Disulfide bondi214 ↔ 240By similarity
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96MU8.
PRIDEiQ96MU8.

PTM databases

PhosphoSiteiQ96MU8.

Expressioni

Gene expression databases

BgeeiQ96MU8.
ExpressionAtlasiQ96MU8. baseline.
GenevisibleiQ96MU8. HS.

Organism-specific databases

HPAiHPA055946.

Interactioni

Protein-protein interaction databases

DIPiDIP-46462N.

Structurei

3D structure databases

ProteinModelPortaliQ96MU8.
SMRiQ96MU8. Positions 31-178, 214-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 11484KringlePROSITE-ProRule annotationAdd
BLAST
Domaini116 – 21095WSCPROSITE-ProRule annotationAdd
BLAST
Domaini214 – 321108CUBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 WSC domain.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262750.
GeneTreeiENSGT00730000110973.
HOVERGENiHBG052290.
InParanoidiQ96MU8.
OMAiPECFTAN.
OrthoDBiEOG7T1RB2.
PhylomeDBiQ96MU8.
TreeFamiTF331319.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR017076. Kremen.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR002889. WSC_carb-bd.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF00051. Kringle. 1 hit.
PF01822. WSC. 1 hit.
[Graphical view]
PIRSFiPIRSF036961. Kremen. 1 hit.
SMARTiSM00042. CUB. 1 hit.
SM00130. KR. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS51212. WSC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96MU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPAARLAL LSAAALTLAA RPAPSPGLGP ECFTANGADY RGTQNWTALQ
60 70 80 90 100
GGKPCLFWNE TFQHPYNTLK YPNGEGGLGE HNYCRNPDGD VSPWCYVAEH
110 120 130 140 150
EDGVYWKYCE IPACQMPGNL GCYKDHGNPP PLTGTSKTSN KLTIQTCISF
160 170 180 190 200
CRSQRFKFAG MESGYACFCG NNPDYWKYGE AASTECNSVC FGDHTQPCGG
210 220 230 240 250
DGRIILFDTL VGACGGNYSA MSSVVYSPDF PDTYATGRVC YWTIRVPGAS
260 270 280 290 300
HIHFSFPLFD IRDSADMVEL LDGYTHRVLA RFHGRSRPPL SFNVSLDFVI
310 320 330 340 350
LYFFSDRINQ AQGFAVLYQA VKEELPQERP AVNQTVAEVI TEQANLSVSA
360 370 380 390 400
ARSSKVLYVI TTSPSHPPQT VPGSNSWAPP MGAGSHRVEG WTVYGLATLL
410 420 430 440 450
ILTVTAIVAK ILLHVTFKSH RVPASGDLRD CHQPGTSGEI WSIFYKPSTS
460 470
ISIFKKKLKG QSQQDDRNPL VSD
Note: No experimental confirmation available. Exon 1 splicing donor site is not canonical.
Length:473
Mass (Da):51,744
Last modified:July 22, 2008 - v3
Checksum:iF6D30DDE708C186B
GO
Isoform 2 (identifier: Q96MU8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     471-473: VSD → AIQDSEVTSLIWSQGQPRSI

Note: No experimental confirmation available.
Show »
Length:492
Mass (Da):53,795
Checksum:iBCCDBC62C07AFF56
GO
Isoform 3 (identifier: Q96MU8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: E → GPE
     373-389: Missing.

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):50,177
Checksum:i8A56506A033E1D4C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2041I → V in BAB71180 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei31 – 311E → GPE in isoform 2 and isoform 3. 2 PublicationsVSP_034914
Alternative sequencei373 – 38917Missing in isoform 3. 1 PublicationVSP_015698Add
BLAST
Alternative sequencei471 – 4733VSD → AIQDSEVTSLIWSQGQPRSI in isoform 2. 1 PublicationVSP_003900

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059618 mRNA. Translation: BAB40969.1.
AK056425 mRNA. Translation: BAB71180.1.
AL021393, Z95116 Genomic DNA. Translation: CAM25378.1.
AL021393, Z95116 Genomic DNA. Translation: CAQ07689.1.
AL021393, Z95116 Genomic DNA. Translation: CAQ07690.1.
Z95116, AL021393 Genomic DNA. Translation: CAM25375.1.
Z95116, AL021393 Genomic DNA. Translation: CAQ08609.1.
Z95116, AL021393 Genomic DNA. Translation: CAQ08610.1.
CH471095 Genomic DNA. Translation: EAW59774.1.
BC063787 mRNA. Translation: AAH63787.1.
CCDSiCCDS13849.1. [Q96MU8-2]
CCDS43000.2. [Q96MU8-3]
RefSeqiNP_001034659.2. NM_001039570.2. [Q96MU8-3]
NP_114434.3. NM_032045.4. [Q96MU8-2]
UniGeneiHs.229335.

Genome annotation databases

EnsembliENST00000327813; ENSP00000331242; ENSG00000183762. [Q96MU8-2]
ENST00000400335; ENSP00000383189; ENSG00000183762. [Q96MU8-3]
ENST00000407188; ENSP00000385431; ENSG00000183762. [Q96MU8-1]
GeneIDi83999.
KEGGihsa:83999.
UCSCiuc003ael.3. human. [Q96MU8-3]
uc011akm.1. human. [Q96MU8-2]
uc011akn.2. human. [Q96MU8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059618 mRNA. Translation: BAB40969.1.
AK056425 mRNA. Translation: BAB71180.1.
AL021393, Z95116 Genomic DNA. Translation: CAM25378.1.
AL021393, Z95116 Genomic DNA. Translation: CAQ07689.1.
AL021393, Z95116 Genomic DNA. Translation: CAQ07690.1.
Z95116, AL021393 Genomic DNA. Translation: CAM25375.1.
Z95116, AL021393 Genomic DNA. Translation: CAQ08609.1.
Z95116, AL021393 Genomic DNA. Translation: CAQ08610.1.
CH471095 Genomic DNA. Translation: EAW59774.1.
BC063787 mRNA. Translation: AAH63787.1.
CCDSiCCDS13849.1. [Q96MU8-2]
CCDS43000.2. [Q96MU8-3]
RefSeqiNP_001034659.2. NM_001039570.2. [Q96MU8-3]
NP_114434.3. NM_032045.4. [Q96MU8-2]
UniGeneiHs.229335.

3D structure databases

ProteinModelPortaliQ96MU8.
SMRiQ96MU8. Positions 31-178, 214-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46462N.

PTM databases

PhosphoSiteiQ96MU8.

Polymorphism and mutation databases

BioMutaiKREMEN1.
DMDMi212287927.

Proteomic databases

PaxDbiQ96MU8.
PRIDEiQ96MU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327813; ENSP00000331242; ENSG00000183762. [Q96MU8-2]
ENST00000400335; ENSP00000383189; ENSG00000183762. [Q96MU8-3]
ENST00000407188; ENSP00000385431; ENSG00000183762. [Q96MU8-1]
GeneIDi83999.
KEGGihsa:83999.
UCSCiuc003ael.3. human. [Q96MU8-3]
uc011akm.1. human. [Q96MU8-2]
uc011akn.2. human. [Q96MU8-1]

Organism-specific databases

CTDi83999.
GeneCardsiGC22P029469.
HGNCiHGNC:17550. KREMEN1.
HPAiHPA055946.
MIMi609898. gene.
neXtProtiNX_Q96MU8.
PharmGKBiPA38241.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG262750.
GeneTreeiENSGT00730000110973.
HOVERGENiHBG052290.
InParanoidiQ96MU8.
OMAiPECFTAN.
OrthoDBiEOG7T1RB2.
PhylomeDBiQ96MU8.
TreeFamiTF331319.

Enzyme and pathway databases

ReactomeiREACT_264383. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_264594. misspliced LRP5 mutants have enhanced beta-catenin-dependent signaling.
REACT_264596. TCF dependent signaling in response to WNT.
SignaLinkiQ96MU8.

Miscellaneous databases

ChiTaRSiKREMEN1. human.
GeneWikiiKREMEN1.
GenomeRNAii83999.
NextBioi73160.
PROiQ96MU8.
SOURCEiSearch...

Gene expression databases

BgeeiQ96MU8.
ExpressionAtlasiQ96MU8. baseline.
GenevisibleiQ96MU8. HS.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR017076. Kremen.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR002889. WSC_carb-bd.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF00051. Kringle. 1 hit.
PF01822. WSC. 1 hit.
[Graphical view]
PIRSFiPIRSF036961. Kremen. 1 hit.
SMARTiSM00042. CUB. 1 hit.
SM00130. KR. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS51212. WSC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Nakamura T., Nakamura T.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Eye.

Entry informationi

Entry nameiKREM1_HUMAN
AccessioniPrimary (citable) accession number: Q96MU8
Secondary accession number(s): B0QY46
, B0QY47, B1AJR5, Q5TIB9, Q6P3X6, Q9BY70, Q9UGS5, Q9UGU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: July 22, 2008
Last modified: June 24, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.