Q96MT1 (RN145_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RING finger protein 145

Gene names

Name:

RNF145

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 RING-type zinc finger.
Sequence caution	The sequence AK308394 differs from that shown. Reason: Frameshift at position 189.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MT1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96MT1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MMRNHRIASSLCGDQVFSKKKKKKKKNNM

Isoform 3 (identifier: Q96MT1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAEVVFSKKKKKKKKNNM

Note: No experimental confirmation available.

Isoform 4 (identifier: Q96MT1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVFSKKKKKKKKNNM

Note: No experimental confirmation available.

Isoform 5 (identifier: Q96MT1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MHRDRISPSNSPTWSLQVFSKKKKKKKKNNM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	RING finger protein 145		PRO_0000294024
Regions
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Transmembrane	205 – 222	18	Helical; Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Transmembrane	275 – 295	21	Helical; Potential
	Transmembrane	316 – 336	21	Helical; Potential
	Transmembrane	340 – 360	21	Helical; Potential
	Transmembrane	384 – 404	21	Helical; Potential
	Transmembrane	410 – 430	21	Helical; Potential
	Transmembrane	460 – 480	21	Helical; Potential
	Transmembrane	482 – 502	21	Helical; Potential
	Zinc finger	537 – 575	39	RING-type; atypical
Natural variations
	Alternative sequence	1	1	M → MMRNHRIASSLCGDQVFSKK KKKKKKNNM in isoform 2.		VSP_037501
	Alternative sequence	1	1	M → MAEVVFSKKKKKKKKNNM in isoform 3.		VSP_043661
	Alternative sequence	1	1	M → MVFSKKKKKKKKNNM in isoform 4.		VSP_043662
	Alternative sequence	1	1	M → MHRDRISPSNSPTWSLQVFS KKKKKKKKNNM in isoform 5.		VSP_044539
Experimental info
	Sequence conflict	9	1	A → V in AAH42684. Ref.3
	Sequence conflict	383	1	V → A in AAH42684. Ref.3
	Sequence conflict	501	1	R → W in AK308394. Ref.1
	Sequence conflict	657	1	A → V in AAH42684. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: EAE984696500ABD3	FASTA	663	75,617
	10 20 30 40 50 60 MAAKEKLEAV LNVALRVPSI MLLDVLYRWD VSSFFQQIQR SSLSNNPLFQ YKYLALNMHY 70 80 90 100 110 120 VGYILSVVLL TLPRQHLVQL YLYFLTALLL YAGHQISRDY VRSELEFAYE GPMYLEPLSM 130 140 150 160 170 180 NRFTTALIGQ LVVCTLCSCV MKTKQIWLFS AHMLPLLARL CLVPLETIVI INKFAMIFTG 190 200 210 220 230 240 LEVLYFLGSN LLVPYNLAKS AYRELVQVVE VYGLLALGMS LWNQLVVPVL FMVFWLVLFA 250 260 270 280 290 300 LQIYSYFSTR DQPASRERLL FLFLTSIAEC CSTPYSLLGL VFTVSFVALG VLTLCKFYLQ 310 320 330 340 350 360 GYRAFMNDPA MNRGMTEGVT LLILAVQTGL IELQVVHRAF LLSIILFIVV ASILQSMLEI 370 380 390 400 410 420 ADPIVLALGA SRDKSLWKHF RAVSLCLFLL VFPAYMAYMI CQFFHMDFWL LIIISSSILT 430 440 450 460 470 480 SLQVLGTLFI YVLFMVEEFR KEPVENMDDV IYYVNGTYRL LEFLVALCVV AYGVSETIFG 490 500 510 520 530 540 EWTVMGSMII FIHSYYNVWL RAQLGWKSFL LRRDAVNKIK SLPIATKEQL EKHNDICAIC 550 560 570 580 590 600 YQDMKSAVIT PCSHFFHAGC LKKWLYVQET CPLCHCHLKN SSQLPGLGTE PVLQPHAGAE 610 620 630 640 650 660 QNVMFQEGTE PPGQEHTPGT RIQEGSRDNN EYIARRPDNQ EGAFDPKEYP HSAKDEAHPV ESA
	Isoform 2 [UniParc]. Checksum: B981A095846EB21D	FASTA	691	78,905
	10 20 30 40 50 60 MMRNHRIASS LCGDQVFSKK KKKKKKNNMA AKEKLEAVLN VALRVPSIML LDVLYRWDVS 70 80 90 100 110 120 SFFQQIQRSS LSNNPLFQYK YLALNMHYVG YILSVVLLTL PRQHLVQLYL YFLTALLLYA 130 140 150 160 170 180 GHQISRDYVR SELEFAYEGP MYLEPLSMNR FTTALIGQLV VCTLCSCVMK TKQIWLFSAH 190 200 210 220 230 240 MLPLLARLCL VPLETIVIIN KFAMIFTGLE VLYFLGSNLL VPYNLAKSAY RELVQVVEVY 250 260 270 280 290 300 GLLALGMSLW NQLVVPVLFM VFWLVLFALQ IYSYFSTRDQ PASRERLLFL FLTSIAECCS 310 320 330 340 350 360 TPYSLLGLVF TVSFVALGVL TLCKFYLQGY RAFMNDPAMN RGMTEGVTLL ILAVQTGLIE 370 380 390 400 410 420 LQVVHRAFLL SIILFIVVAS ILQSMLEIAD PIVLALGASR DKSLWKHFRA VSLCLFLLVF 430 440 450 460 470 480 PAYMAYMICQ FFHMDFWLLI IISSSILTSL QVLGTLFIYV LFMVEEFRKE PVENMDDVIY 490 500 510 520 530 540 YVNGTYRLLE FLVALCVVAY GVSETIFGEW TVMGSMIIFI HSYYNVWLRA QLGWKSFLLR 550 560 570 580 590 600 RDAVNKIKSL PIATKEQLEK HNDICAICYQ DMKSAVITPC SHFFHAGCLK KWLYVQETCP 610 620 630 640 650 660 LCHCHLKNSS QLPGLGTEPV LQPHAGAEQN VMFQEGTEPP GQEHTPGTRI QEGSRDNNEY 670 680 690 IARRPDNQEG AFDPKEYPHS AKDEAHPVES A
	Isoform 3 [UniParc]. Checksum: C1F1EC3ECDDD18CC	FASTA	680	77,635
	10 20 30 40 50 60 MAEVVFSKKK KKKKKNNMAA KEKLEAVLNV ALRVPSIMLL DVLYRWDVSS FFQQIQRSSL 70 80 90 100 110 120 SNNPLFQYKY LALNMHYVGY ILSVVLLTLP RQHLVQLYLY FLTALLLYAG HQISRDYVRS 130 140 150 160 170 180 ELEFAYEGPM YLEPLSMNRF TTALIGQLVV CTLCSCVMKT KQIWLFSAHM LPLLARLCLV 190 200 210 220 230 240 PLETIVIINK FAMIFTGLEV LYFLGSNLLV PYNLAKSAYR ELVQVVEVYG LLALGMSLWN 250 260 270 280 290 300 QLVVPVLFMV FWLVLFALQI YSYFSTRDQP ASRERLLFLF LTSIAECCST PYSLLGLVFT 310 320 330 340 350 360 VSFVALGVLT LCKFYLQGYR AFMNDPAMNR GMTEGVTLLI LAVQTGLIEL QVVHRAFLLS 370 380 390 400 410 420 IILFIVVASI LQSMLEIADP IVLALGASRD KSLWKHFRAV SLCLFLLVFP AYMAYMICQF 430 440 450 460 470 480 FHMDFWLLII ISSSILTSLQ VLGTLFIYVL FMVEEFRKEP VENMDDVIYY VNGTYRLLEF 490 500 510 520 530 540 LVALCVVAYG VSETIFGEWT VMGSMIIFIH SYYNVWLRAQ LGWKSFLLRR DAVNKIKSLP 550 560 570 580 590 600 IATKEQLEKH NDICAICYQD MKSAVITPCS HFFHAGCLKK WLYVQETCPL CHCHLKNSSQ 610 620 630 640 650 660 LPGLGTEPVL QPHAGAEQNV MFQEGTEPPG QEHTPGTRIQ EGSRDNNEYI ARRPDNQEGA 670 680 FDPKEYPHSA KDEAHPVESA
	Isoform 4 [UniParc]. Checksum: 14F56CB45C03474E	FASTA	677	77,335
	10 20 30 40 50 60 MVFSKKKKKK KKNNMAAKEK LEAVLNVALR VPSIMLLDVL YRWDVSSFFQ QIQRSSLSNN 70 80 90 100 110 120 PLFQYKYLAL NMHYVGYILS VVLLTLPRQH LVQLYLYFLT ALLLYAGHQI SRDYVRSELE 130 140 150 160 170 180 FAYEGPMYLE PLSMNRFTTA LIGQLVVCTL CSCVMKTKQI WLFSAHMLPL LARLCLVPLE 190 200 210 220 230 240 TIVIINKFAM IFTGLEVLYF LGSNLLVPYN LAKSAYRELV QVVEVYGLLA LGMSLWNQLV 250 260 270 280 290 300 VPVLFMVFWL VLFALQIYSY FSTRDQPASR ERLLFLFLTS IAECCSTPYS LLGLVFTVSF 310 320 330 340 350 360 VALGVLTLCK FYLQGYRAFM NDPAMNRGMT EGVTLLILAV QTGLIELQVV HRAFLLSIIL 370 380 390 400 410 420 FIVVASILQS MLEIADPIVL ALGASRDKSL WKHFRAVSLC LFLLVFPAYM AYMICQFFHM 430 440 450 460 470 480 DFWLLIIISS SILTSLQVLG TLFIYVLFMV EEFRKEPVEN MDDVIYYVNG TYRLLEFLVA 490 500 510 520 530 540 LCVVAYGVSE TIFGEWTVMG SMIIFIHSYY NVWLRAQLGW KSFLLRRDAV NKIKSLPIAT 550 560 570 580 590 600 KEQLEKHNDI CAICYQDMKS AVITPCSHFF HAGCLKKWLY VQETCPLCHC HLKNSSQLPG 610 620 630 640 650 660 LGTEPVLQPH AGAEQNVMFQ EGTEPPGQEH TPGTRIQEGS RDNNEYIARR PDNQEGAFDP 670 KEYPHSAKDE AHPVESA
	Isoform 5 [UniParc]. Checksum: B31C1F06B683C778	FASTA	693	79,198
	10 20 30 40 50 60 MHRDRISPSN SPTWSLQVFS KKKKKKKKNN MAAKEKLEAV LNVALRVPSI MLLDVLYRWD 70 80 90 100 110 120 VSSFFQQIQR SSLSNNPLFQ YKYLALNMHY VGYILSVVLL TLPRQHLVQL YLYFLTALLL 130 140 150 160 170 180 YAGHQISRDY VRSELEFAYE GPMYLEPLSM NRFTTALIGQ LVVCTLCSCV MKTKQIWLFS 190 200 210 220 230 240 AHMLPLLARL CLVPLETIVI INKFAMIFTG LEVLYFLGSN LLVPYNLAKS AYRELVQVVE 250 260 270 280 290 300 VYGLLALGMS LWNQLVVPVL FMVFWLVLFA LQIYSYFSTR DQPASRERLL FLFLTSIAEC 310 320 330 340 350 360 CSTPYSLLGL VFTVSFVALG VLTLCKFYLQ GYRAFMNDPA MNRGMTEGVT LLILAVQTGL 370 380 390 400 410 420 IELQVVHRAF LLSIILFIVV ASILQSMLEI ADPIVLALGA SRDKSLWKHF RAVSLCLFLL 430 440 450 460 470 480 VFPAYMAYMI CQFFHMDFWL LIIISSSILT SLQVLGTLFI YVLFMVEEFR KEPVENMDDV 490 500 510 520 530 540 IYYVNGTYRL LEFLVALCVV AYGVSETIFG EWTVMGSMII FIHSYYNVWL RAQLGWKSFL 550 560 570 580 590 600 LRRDAVNKIK SLPIATKEQL EKHNDICAIC YQDMKSAVIT PCSHFFHAGC LKKWLYVQET 610 620 630 640 650 660 CPLCHCHLKN SSQLPGLGTE PVLQPHAGAE QNVMFQEGTE PPGQEHTPGT RIQEGSRDNN 670 680 690 EYIARRPDNQ EGAFDPKEYP HSAKDEAHPV ESA

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4 AND 5). Tissue: Trachea.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056513 mRNA. Translation: BAB71200.1. AK304228 mRNA. Translation: BAH14139.1. AK304435 mRNA. Translation: BAH14185.1. AK308394 mRNA. No translation available. AC134043 Genomic DNA. No translation available. BC042684 mRNA. Translation: AAH42684.1.
IPI	IPI00043360. IPI00930398. IPI00975659. IPI00976259. IPI00979590.
RefSeq	NP_001186309.1. NM_001199380.1. NP_001186310.1. NM_001199381.1. NP_001186311.1. NM_001199382.1. NP_001186312.1. NM_001199383.1. NP_653327.1. NM_144726.2.
UniGene	Hs.349306.
3D structure databases
HSSP	HSSP built from PDB template 1X4J based on UniProtKB Q9H0F5.
ProteinModelPortal	Q96MT1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274542.
PTM databases
PhosphoSite	Q96MT1.
Polymorphism databases
DMDM	152060502.
Proteomic databases
PaxDb	Q96MT1.
PRIDE	Q96MT1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274542; ENSP00000274542; ENSG00000145860. ENST00000413445; ENSP00000445115; ENSG00000145860. ENST00000424310; ENSP00000409064; ENSG00000145860. ENST00000518802; ENSP00000430955; ENSG00000145860. ENST00000519865; ENSP00000430397; ENSG00000145860. ENST00000520638; ENSP00000429071; ENSG00000145860. ENST00000535312; ENSP00000442941; ENSG00000145860.
GeneID	153830.
KEGG	hsa:153830.
UCSC	uc003lxo.2. human. uc003lxp.3. human.
Organism-specific databases
CTD	153830.
GeneCards	GC05M158517.
HGNC	HGNC:20853. RNF145.
HPA	HPA036562.
neXtProt	NX_Q96MT1.
PharmGKB	PA134876286.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG246550.
HOGENOM	HOG000072697.
HOVERGEN	HBG054921.
InParanoid	Q96MT1.
OMA	AGAEQNV.
OrthoDB	EOG4BCDMJ.
Gene expression databases
ArrayExpress	Q96MT1.
Bgee	Q96MT1.
CleanEx	HS_RNF145.
Genevestigator	Q96MT1.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR025754. TRC8_N_dom. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF13705. TRC8_N. 1 hit. PF13639. zf-RING_2. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	153830.
NextBio	87196.

Entry information

Entry name

RN145_HUMAN

Accession

Primary (citable) accession number: Q96MT1
Secondary accession number(s): B7Z903 , B7Z949, E7EVI7, Q8IVP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.