Q96MM7 (H6ST2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2
Short name=HS6ST-2
EC=2.8.2.-

Gene names

Name:	HS6ST2
ORF Names:	PSEC0092

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the sulfotransferase 6 family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome glycosaminoglycan biosynthetic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sulfotransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96MM7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96MM7-2)

Also known as: HS6ST-2S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Isoform 3 (identifier: Q96MM7-3)

Also known as: HS6ST-2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.
     315-316: SR → SRWRIFQILDAASKDKRGSPNTNAGANSPSSTKTRNTSKSGK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Heparan-sulfate 6-O-sulfotransferase 2		PRO_0000190805
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 27	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 605	578	Lumenal Potential
	Region	236 – 242	7	5'-phosphosulfate-binding Potential
	Region	325 – 333	9	3'-phosphate binding Potential
Amino acid modifications
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
	Glycosylation	544	1	N-linked (GlcNAc...) Potential
	Glycosylation	556	1	N-linked (GlcNAc...) Potential
	Glycosylation	564	1	N-linked (GlcNAc...) Potential
	Glycosylation	589	1	N-linked (GlcNAc...) Potential
	Glycosylation	592	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 146	146	Missing in isoform 2 and isoform 3.		VSP_015846
	Alternative sequence	315 – 316	2	SR → SRWRIFQILDAASKDKRGSP NTNAGANSPSSTKTRNTSKS GK in isoform 3.		VSP_015847
	Natural variant	127	1	K → N. Corresponds to variant rs7053397 [ dbSNP | Ensembl ].		VAR_061828
Experimental info
	Sequence conflict	192	1	D → G in BAB71260. Ref.2
	Sequence conflict	299	1	C → Y in AAH37325. Ref.4
	Sequence conflict	426	1	K → R in BAC11597. Ref.5
	Sequence conflict	568	1	S → N in BAB71260. Ref.2
	Sequence conflict	580	1	Q → R in BAC11597. Ref.5
	Sequence conflict	585	1	E → G in BAC11597. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: 10528AC424935B13	FASTA	605	69,130
	10 20 30 40 50 60 MALPACAVRE FEPPRQPERG APVRTTCPRR HSRVEAELAA SRPGSVAASV RAGPPRGVSH 70 80 90 100 110 120 GFHTRPLLDK PRKASSSLAG AACAPLFALL SRGRRRRMHV LRRRWDLGSL CRALLTRGLA 130 140 150 160 170 180 ALGHSLKHVL GAIFSKIFGP MASVGNMDEK SNKLLLALVM LFLFAVIVLQ YVCPGTECQL 190 200 210 220 230 240 LRLQAFSSPV PDPYRSEDES SARFVPRYNF TRGDLLRKVD FDIKGDDLIV FLHIQKTGGT 250 260 270 280 290 300 TFGRHLVRNI QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV 310 320 330 340 350 360 PSVVDGKRDA RLRPSRNFHY ITILRDPVSR YLSEWRHVQR GATWKASLHV CDGRPPTSEE 370 380 390 400 410 420 LPSCYTGDDW SGCPLKEFMD CPYNLANNRQ VRMLSDLTLV GCYNLSVMPE KQRNKVLLES 430 440 450 460 470 480 AKSNLKHMAF FGLTEFQRKT QYLFEKTFNM NFISPFTQYN TTRASSVEIN EEIQKRIEGL 490 500 510 520 530 540 NFLDMELYSY AKDLFLQRYQ FMRQKEHQEA RRKRQEQRKF LKGRLLQTHF QSQGQGQSQN 550 560 570 580 590 600 PNQNQSQNPN PNANQNLTQN LMQNLTQSLS QKENRESPKQ NSGKEQNDNT SNGTNDYIGS VEKWR
	Isoform 2 (HS6ST-2S) [UniParc]. Checksum: 74B35C8D212BA566	FASTA	459	53,436
	10 20 30 40 50 60 MDEKSNKLLL ALVMLFLFAV IVLQYVCPGT ECQLLRLQAF SSPVPDPYRS EDESSARFVP 70 80 90 100 110 120 RYNFTRGDLL RKVDFDIKGD DLIVFLHIQK TGGTTFGRHL VRNIQLEQPC ECRVGQKKCT 130 140 150 160 170 180 CHRPGKRETW LFSRFSTGWS CGLHADWTEL TSCVPSVVDG KRDARLRPSR NFHYITILRD 190 200 210 220 230 240 PVSRYLSEWR HVQRGATWKA SLHVCDGRPP TSEELPSCYT GDDWSGCPLK EFMDCPYNLA 250 260 270 280 290 300 NNRQVRMLSD LTLVGCYNLS VMPEKQRNKV LLESAKSNLK HMAFFGLTEF QRKTQYLFEK 310 320 330 340 350 360 TFNMNFISPF TQYNTTRASS VEINEEIQKR IEGLNFLDME LYSYAKDLFL QRYQFMRQKE 370 380 390 400 410 420 HQEARRKRQE QRKFLKGRLL QTHFQSQGQG QSQNPNQNQS QNPNPNANQN LTQNLMQNLT 430 440 450 QSLSQKENRE SPKQNSGKEQ NDNTSNGTND YIGSVEKWR
	Isoform 3 (HS6ST-2) [UniParc]. Checksum: FE81141A7FF0B9E7	FASTA	499	57,697
	10 20 30 40 50 60 MDEKSNKLLL ALVMLFLFAV IVLQYVCPGT ECQLLRLQAF SSPVPDPYRS EDESSARFVP 70 80 90 100 110 120 RYNFTRGDLL RKVDFDIKGD DLIVFLHIQK TGGTTFGRHL VRNIQLEQPC ECRVGQKKCT 130 140 150 160 170 180 CHRPGKRETW LFSRFSTGWS CGLHADWTEL TSCVPSVVDG KRDARLRPSR WRIFQILDAA 190 200 210 220 230 240 SKDKRGSPNT NAGANSPSST KTRNTSKSGK NFHYITILRD PVSRYLSEWR HVQRGATWKA 250 260 270 280 290 300 SLHVCDGRPP TSEELPSCYT GDDWSGCPLK EFMDCPYNLA NNRQVRMLSD LTLVGCYNLS 310 320 330 340 350 360 VMPEKQRNKV LLESAKSNLK HMAFFGLTEF QRKTQYLFEK TFNMNFISPF TQYNTTRASS 370 380 390 400 410 420 VEINEEIQKR IEGLNFLDME LYSYAKDLFL QRYQFMRQKE HQEARRKRQE QRKFLKGRLL 430 440 450 460 470 480 QTHFQSQGQG QSQNPNQNQS QNPNPNANQN LTQNLMQNLT QSLSQKENRE SPKQNSGKEQ 490 NDNTSNGTND YIGSVEKWR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of heparan sulphate with diverse structures and functions: two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties." Habuchi H., Miyake G., Nogami K., Kuroiwa A., Matsuda Y., Kusche-Gullberg M., Habuchi O., Tanaka M., Kimata K. Biochem. J. 371:131-142(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-605 (ISOFORM 3). Tissue: Placenta.
[5]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-605. Tissue: Teratocarcinoma.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 465-605. Tissue: Amygdala.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB067776 mRNA. Translation: BAC07183.1. AB067777 mRNA. Translation: BAC07184.1. AK027720 mRNA. Translation: BAB55322.1. AK056706 mRNA. Translation: BAB71260.1. Z81365, Z86064 Genomic DNA. Translation: CAX30811.1. Z81365, Z86064 Genomic DNA. Translation: CAX30812.1. AL022309 Genomic DNA. No translation available. AL022159 Genomic DNA. No translation available. Z82205 Genomic DNA. No translation available. Z86064, Z81365 Genomic DNA. Translation: CAI42774.1. Z86064, Z81365 Genomic DNA. Translation: CAI42775.1. BC037325 mRNA. Translation: AAH37325.1. BC094718 mRNA. Translation: AAH94718.1. BC110620 mRNA. Translation: AAI10621.1. BC110621 mRNA. Translation: AAI10622.1. AK075402 mRNA. Translation: BAC11597.1. AL831923 mRNA. Translation: CAD38583.1.
IPI	IPI00157454. IPI00160316. IPI00937318.
RefSeq	NP_001070656.1. NM_001077188.1. NP_671704.3. NM_147175.3.
UniGene	Hs.385956.
3D structure databases
ProteinModelPortal	Q96MM7.
ModBase	Search...
PTM databases
PhosphoSite	Q96MM7.
Polymorphism databases
DMDM	77416506.
Proteomic databases
PaxDb	Q96MM7.
PRIDE	Q96MM7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370836; ENSP00000359873; ENSG00000171004. ENST00000370837; ENSP00000359874; ENSG00000171004. ENST00000461959; ENSP00000435108; ENSG00000171004.
GeneID	90161.
KEGG	hsa:90161.
UCSC	uc011mvb.1. human. uc011mvc.1. human.
Organism-specific databases
CTD	90161.
GeneCards	GC0XM131760.
HGNC	HGNC:19133. HS6ST2.
HPA	HPA034625.
MIM	300545. gene.
neXtProt	NX_Q96MM7.
PharmGKB	PA134950831.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG302961.
HOVERGEN	HBG083012.
KO	K08102.
OrthoDB	EOG4X6C8G.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	Q96MM7.
Bgee	Q96MM7.
CleanEx	HS_HS6ST2.
Genevestigator	Q96MM7.
GermOnline	ENSG00000171004. Homo sapiens.
Family and domain databases
InterPro	IPR010635. Heparan_SO4-6-sulfoTrfase. IPR005331. Sulfotransferase. [Graphical view]
PANTHER	PTHR12812. PTHR12812. 1 hit.
Pfam	PF03567. Sulfotransfer_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HS6ST2. human.
GenomeRNAi	90161.
NextBio	76561.
SOURCE	Search...

Entry information

Entry name

H6ST2_HUMAN

Accession

Primary (citable) accession number: Q96MM7
Secondary accession number(s): B9WRT4 , B9WRT5, Q2TB13, Q4VC07, Q6PIC4, Q86SM9, Q8N3T4, Q8NBN4, Q96SJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.