ID   HS12B_HUMAN             Reviewed;         686 AA.
AC   Q96MM6; D3DVX7; Q2TAK3; Q9BR52;
DT   10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2003, sequence version 2.
DT   27-MAR-2024, entry version 147.
DE   RecName: Full=Heat shock 70 kDa protein 12B;
GN   Name=HSPA12B; Synonyms=C20orf60;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11780052; DOI=10.1038/414865a;
RA   Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA   Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA   Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA   Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA   Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA   Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA   Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA   Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA   Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA   Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA   Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA   Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA   Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA   Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA   Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA   Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA   Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA   Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA   Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA   Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA   Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 20.";
RL   Nature 414:865-871(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, and Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   IDENTIFICATION, AND TISSUE SPECIFICITY.
RX   PubMed=12552099; DOI=10.1073/pnas.252764399;
RA   Han Z., Truong Q.A., Park S., Breslow J.L.;
RT   "Two Hsp70 family members expressed in atherosclerotic lesions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1256-1261(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-29 AND SER-276, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- INTERACTION:
CC       Q96MM6; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-10291310, EBI-3867333;
CC       Q96MM6; O95967: EFEMP2; NbExp=3; IntAct=EBI-10291310, EBI-743414;
CC       Q96MM6; O43301: HSPA12A; NbExp=3; IntAct=EBI-10291310, EBI-296980;
CC       Q96MM6; Q15323: KRT31; NbExp=6; IntAct=EBI-10291310, EBI-948001;
CC       Q96MM6; O76011: KRT34; NbExp=3; IntAct=EBI-10291310, EBI-1047093;
CC       Q96MM6; Q6A162: KRT40; NbExp=3; IntAct=EBI-10291310, EBI-10171697;
CC       Q96MM6; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-10291310, EBI-10171774;
CC       Q96MM6; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-10291310, EBI-945833;
CC       Q96MM6; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-10291310, EBI-22310682;
CC       Q96MM6; Q12933: TRAF2; NbExp=3; IntAct=EBI-10291310, EBI-355744;
CC   -!- TISSUE SPECIFICITY: Highest expression in muscle and heart. Lower
CC       levels in liver and kidney. {ECO:0000269|PubMed:12552099}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; AK056712; BAB71261.1; -; mRNA.
DR   EMBL; AL109804; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471133; EAX10516.1; -; Genomic_DNA.
DR   EMBL; BC110881; AAI10882.1; -; mRNA.
DR   EMBL; BC117284; AAI17285.1; -; mRNA.
DR   EMBL; BC126263; AAI26264.1; -; mRNA.
DR   CCDS; CCDS13061.1; -.
DR   RefSeq; NP_001184256.1; NM_001197327.1.
DR   RefSeq; NP_443202.3; NM_052970.4.
DR   RefSeq; XP_016883121.1; XM_017027632.1.
DR   AlphaFoldDB; Q96MM6; -.
DR   BioGRID; 125532; 73.
DR   IntAct; Q96MM6; 12.
DR   STRING; 9606.ENSP00000254963; -.
DR   iPTMnet; Q96MM6; -.
DR   PhosphoSitePlus; Q96MM6; -.
DR   BioMuta; HSPA12B; -.
DR   DMDM; 27734244; -.
DR   EPD; Q96MM6; -.
DR   jPOST; Q96MM6; -.
DR   MassIVE; Q96MM6; -.
DR   PaxDb; 9606-ENSP00000254963; -.
DR   PeptideAtlas; Q96MM6; -.
DR   ProteomicsDB; 77374; -.
DR   Antibodypedia; 2863; 142 antibodies from 24 providers.
DR   DNASU; 116835; -.
DR   Ensembl; ENST00000254963.7; ENSP00000254963.2; ENSG00000132622.11.
DR   GeneID; 116835; -.
DR   KEGG; hsa:116835; -.
DR   MANE-Select; ENST00000254963.7; ENSP00000254963.2; NM_052970.5; NP_443202.3.
DR   UCSC; uc002wjd.4; human.
DR   AGR; HGNC:16193; -.
DR   CTD; 116835; -.
DR   DisGeNET; 116835; -.
DR   GeneCards; HSPA12B; -.
DR   HGNC; HGNC:16193; HSPA12B.
DR   HPA; ENSG00000132622; Low tissue specificity.
DR   MIM; 610702; gene.
DR   neXtProt; NX_Q96MM6; -.
DR   OpenTargets; ENSG00000132622; -.
DR   PharmGKB; PA25771; -.
DR   VEuPathDB; HostDB:ENSG00000132622; -.
DR   eggNOG; KOG0101; Eukaryota.
DR   GeneTree; ENSGT00940000159032; -.
DR   InParanoid; Q96MM6; -.
DR   OMA; IYKHTME; -.
DR   OrthoDB; 1377056at2759; -.
DR   PhylomeDB; Q96MM6; -.
DR   TreeFam; TF329492; -.
DR   PathwayCommons; Q96MM6; -.
DR   Reactome; R-HSA-3371453; Regulation of HSF1-mediated heat shock response.
DR   SignaLink; Q96MM6; -.
DR   BioGRID-ORCS; 116835; 11 hits in 1143 CRISPR screens.
DR   ChiTaRS; HSPA12B; human.
DR   GenomeRNAi; 116835; -.
DR   Pharos; Q96MM6; Tbio.
DR   PRO; PR:Q96MM6; -.
DR   Proteomes; UP000005640; Chromosome 20.
DR   RNAct; Q96MM6; Protein.
DR   Bgee; ENSG00000132622; Expressed in tendon of biceps brachii and 165 other cell types or tissues.
DR   ExpressionAtlas; Q96MM6; baseline and differential.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.40; -; 2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR14187; ALPHA KINASE/ELONGATION FACTOR 2 KINASE; 1.
DR   PANTHER; PTHR14187:SF39; HEAT SHOCK 70 KDA PROTEIN 12B; 1.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR   Genevisible; Q96MM6; HS.
PE   1: Evidence at protein level;
KW   ATP-binding; Nucleotide-binding; Phosphoprotein; Reference proteome.
FT   CHAIN           1..686
FT                   /note="Heat shock 70 kDa protein 12B"
FT                   /id="PRO_0000078294"
FT   REGION          12..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        30..53
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         25
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         29
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         42
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT   MOD_RES         44
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT   MOD_RES         46
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT   MOD_RES         276
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   VARIANT         23
FT                   /note="V -> L (in dbSNP:rs34414870)"
FT                   /id="VAR_049621"
FT   VARIANT         270
FT                   /note="R -> H (in dbSNP:rs6139194)"
FT                   /id="VAR_059362"
FT   CONFLICT        300
FT                   /note="E -> D (in Ref. 1; BAB71261)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   686 AA;  75688 MW;  5F873AE97E366606 CRC64;
     MLAVPEMGLQ GLYIGSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE VRAPQQASFS
     VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH QKTPTCLLLT PEGAFHSFGY
     TARDYYHDLD PEEARDWLYF EKFKMKIHSA TDLTLKTQLE AVNGKTMPAL EVFAHALRFF
     REHALQELRE QSPSLPEKDT VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS RENAEQLLIA
     LEPEAASVYC RKLRLHQLLD LSGRAPGGGR LGERRSIDSS FRQAREQLRR SRHSRTFLVE
     SGVGELWAEM QAGDRYVVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP YGAVGVDLAF
     EQLLCRIFGE DFIATFKRQR PAAWVDLTIA FEARKRTAGP HRAGALNISL PFSFIDFYRK
     QRGHNVETAL RRSSVNFVKW SSQGMLRMSC EAMNELFQPT VSGIIQHIEA LLARPEVQGV
     KLLFLVGGFA ESAVLQHAVQ AALGARGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP
     LTYGVGVLNR FVPGRHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
     QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPADCG QDTAGAPPGR REIRAAMQFG
     DTEIKVTAVD VSTNRSVRAS IDFLSN
//